BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0004.p (539 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic ... 629 e-180 BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic... 79 2e-14 BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic ... 78 4e-14 BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic ... 75 3e-13 YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic... 68 4e-11 >SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 538 Score = 629 bits (1621), Expect = e-180, Method: Compositional matrix adjust. Identities = 297/488 (60%), Positives = 377/488 (77%), Gaps = 1/488 (0%) Query: 48 KVTLNFITQSSPLAPADPNDKLINKRLEEKTNVHINWKNFTKDVFVEKRNLAVASGDLPD 107 K TL F+T SSPL+P DPN+KLI +RLE++T VHI+W N+ D F EKRNL ++SGDLPD Sbjct: 46 KKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSD-FAEKRNLDISSGDLPD 104 Query: 108 AIFNADYSDYELLKLAKDGTIIPLNDLIEKNMPNFKKVLEEAPEYKSMITAPDGNIYAFP 167 AI N SD +L+ AK G IIP+ DLI+K MPN KK+L+E PEYK+++TAPDG+IY+FP Sbjct: 105 AIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHIYSFP 164 Query: 168 WIEELGNGKERIQAVDSMPWINVEWLKKLGLEMPKTTDELKKVLIAFKTQDPNGNGQADE 227 WIEELG+GKE I +V+ M WIN +WLKKLGLEMPKTTD+L KVL AFK DPNGNG+ADE Sbjct: 165 WIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNGNGEADE 224 Query: 228 IPLSFINKPGAEDLAFLFASFGLGENPDHAVVSDDGKVIFTAAEEGYKNAISFINELYKE 287 IP SFI+ G ED FLFA+FG+G+N DH VV +DGKV FTA + YK + FI +L ++ Sbjct: 225 IPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVKFIRQLQEK 284 Query: 288 GLIDIEAYTQDWSTYLAKGKEQRYGLYFSWDKANISGANDAYDVMPPLAGPDGEVNVTRT 347 GLID EA+ DW++Y+AKG +Q++G+YF+WDK N++G+N++YDV+P LAGP G+ +V RT Sbjct: 285 GLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAGPSGQKHVART 344 Query: 348 NGLGLGRGKMVVTSANKNLETTAKWVDQLYDPLQSVQDNWGTYGDETQQNIFELDEAKGM 407 NG+G R KMV+TS NKNLE TAKW+D Y PLQSVQ+NWGTYGD+ QQNIFELD+A Sbjct: 345 NGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQQNIFELDQASNS 404 Query: 408 LKHLPLEGAAPVELREKTSIGGPLAVLDSYYGKYTTMPDDAKGRMDIIKNIMAPKMKAEN 467 LKHLPL G AP ELR+KT +GGPLA+LDSYYGK TTMPDDAK R+D+IK P M N Sbjct: 405 LKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLDLIKEYYVPYMSNVN 464 Query: 468 VMPSVFNSIEELDRLTTIETDLFAYVLRMRTEWYQNGKVNEQWGEYLKELDRLGLQEWLK 527 P VF + E+LD++ IE D+ Y+ R R EW NG ++ +W +Y KEL++ GL ++L Sbjct: 465 NYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWDDYKKELEKYGLSDYLA 524 Query: 528 IKQDGYDR 535 IKQ YD+ Sbjct: 525 IKQKYYDQ 532 >BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 502 Score = 79.0 bits (193), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 96/354 (27%), Positives = 159/354 (44%), Gaps = 49/354 (13%) Query: 60 LAPADPNDKLIN-KRLEEKTNVHINWKNFTKDVFVEKRNLAVASGDLPDAIFNADYSDYE 118 L+P P+DK + K LE+ T I + +K N+ +ASG++P AI D S Sbjct: 45 LSPESPSDKSPSLKALEKYTGKEIKVTWVPDSSYNDKFNIVMASGEMPHAIVIKDKS-AG 103 Query: 119 LLKLAKDGTIIPLNDLIEKNMPNFK--KVLEEAPEYKSMITAPDGNIYAFPWIEELGNGK 176 +K K G L+ P K K L +A E ++ +G +Y +L Sbjct: 104 FIKSVKAGAFWELS-------PYLKDYKNLSQADEKILKNSSVNGEVYGIYRTRDL---- 152 Query: 177 ERIQAVDSMPWINVEWLKKLGLEMPKTTDELKKVLIAFKTQDPNGNGQADE----IP--L 230 + + I +WLK +GL+MP+T D+ +VL AFK +DP+GNG+ D +P + Sbjct: 153 -----IRACMIIRTDWLKNVGLDMPETLDDFYEVLKAFKEKDPDGNGKDDTYGMVVPKWM 207 Query: 231 SFINKPGAEDLAFLFASFGLGENPDHAVVSDDGKVI--FTAAEEGYKNAISFINELYKEG 288 N + L F + P+ V ++GK+I FT E Y +A++F +LY EG Sbjct: 208 GLGNGSPWDVLQIWFGA------PNRYGV-ENGKLIPDFTTKE--YMDALTFFKKLYDEG 258 Query: 289 LIDIEAYTQD---WSTYLAKGK--------EQRYGLYFSWDKANISGANDAYDVMPPLAG 337 LI+ + D W+ + KGK + + + ++A+ S D D++ L G Sbjct: 259 LINKDFAVMDSAKWNDPVVKGKAGVIVDTGSRASQIQSAMEEADESN-KDIIDIVGSLEG 317 Query: 338 PDGEVNVTRTNGLGLGRGKMVVTSANKNLETTAKWVDQLYDPLQSVQDNWGTYG 391 P+G+ + G+ K L+ ++D++ D + N G G Sbjct: 318 PNGKRTFPTSGYSGMITIPKSSVKTEKELKEVLSFLDKMNDKEAQILTNNGVKG 371 >BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 512 Score = 77.8 bits (190), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 89/327 (27%), Positives = 141/327 (43%), Gaps = 54/327 (16%) Query: 44 DPSGKVTLNFITQSSPLAPADPNDKLINKRLEEKTNVHINWKNFTKDVFVEKRNLAVASG 103 DP + L+++ P P D +I K +EE TN +++ V ++ N A+ASG Sbjct: 47 DPDSPLDLDWMVILYHQQP--PKDTVI-KEIEELTNTNLDLIWVPDAVKEDRVNAALASG 103 Query: 104 DLPDAIFNADYSDYELLKLAKDGTIIPLNDLIEKNMPNFKKVLEEAPEYKSMITAPDGNI 163 +LP I D+ +L + G + +++ PN ++ E E S+ DG I Sbjct: 104 NLPKVITLQDFRTSSVLNAFRSGQFWEIGPYLDE-FPNLSEMNETILENVSV----DGKI 158 Query: 164 YAFPWIEELGNGKERIQAVDSMPWINVEWLKKLGLEMPKTTDELKKVLIAFKTQDPNGNG 223 Y G +ER + + I +WL+ LGLEMP +EL +V AF DPNG+G Sbjct: 159 Y--------GIYRERPLSRQGVV-IRQDWLENLGLEMPTNLEELYEVARAFTYDDPNGSG 209 Query: 224 QADEIPLSFINKPGAEDLAFLFASF----GLGENPDHAVVSDDGKVIFTAAEEGYKNAIS 279 D + + N +F +F P+ D+GK++ + Y ++ Sbjct: 210 ADDTVGFTDRND-------LIFGAFKTLASYHGTPNEWGFDDEGKLMPEFMTDEYVETMN 262 Query: 280 FINELYKEGLIDIEAYTQDWSTYLAKGKEQRYGLYFSWDKANISGAN---------DAYD 330 ++ LY EGLI+ E + KEQ+ L+ + +A I N A D Sbjct: 263 YMKSLYDEGLINTE--------FPVTSKEQQQELFMT-GRAGIYVGNMVDARNLRDKAKD 313 Query: 331 VMPPL--------AGPDGEVNVTRTNG 349 + P + AGPDGE V G Sbjct: 314 INPEMEVAITNRIAGPDGEDRVWAQGG 340 >BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 550 Score = 75.1 bits (183), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 62/237 (26%), Positives = 114/237 (48%), Gaps = 21/237 (8%) Query: 64 DPNDKLINKRLEEKTNVHI--NWKNFTKDVFVEKRNLAVASGD-LPDAIFNADYSDYELL 120 D +D ++ + EE+ + W + F + L ++SG+ LPD +F + + + Sbjct: 60 DVHDNVVTRWAEEELGIKFVTEWTQPNDEQFNTQLRLMMSSGEKLPD-VFQVNDTAIKA- 117 Query: 121 KLAKDGTIIPLNDLIEKNM-PNFKKVLEEAPEYKSMITAPDGNIYAFPWIEELGNGKERI 179 L G ++P+++ IE P K++ E+ P+ + DG Y P GNG Sbjct: 118 DLLSSGLVMPVDEAIETYASPRLKQLFEQFPQ-AFYPSTKDGVRYGIPRFSG-GNGS--- 172 Query: 180 QAVDSMPWINVEWLKKLGLEMPKTTDELKKVLIAFKTQDPNGNGQADEIPLSF------I 233 D++ WI +WL K LE P T +EL+ ++ F +DPN G+ D I ++F Sbjct: 173 ---DTLLWIRQDWLDKFDLEPPTTLEELEHIMDVFVNEDPNETGKNDTIGITFAAGDYGF 229 Query: 234 NKPGAEDLAFLFASFGLGENPDHAVVSDDGKVIFTAAEEGYKNAISFINELYKEGLI 290 D +++F +FG P DDG +++ + + K+ ++ I+E Y++G + Sbjct: 230 RGGNIADSSWVFGAFG-DFVPGAWSKGDDGSLVYGSIQPSMKDGLAKIHEWYEKGYL 285 >YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 437 Score = 68.2 bits (165), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 72/304 (23%), Positives = 127/304 (41%), Gaps = 37/304 (12%) Query: 74 LEEKTNVHINWKNFTKDVFVEKRNLAVASGDLPDAIFNADYSDYELLKLAKDGTIIPLND 133 E+KT + I + N T D F ++ A A LPD IFN + + ++L G + P+ Sbjct: 55 FEKKTGIKIEYFNATTD-FEQRLARAAAGNALPDLIFNDAVAIGQFVQL---GIVEPIE- 109 Query: 134 LIEKNMPNFKKVLEEAPEYKSMITAPDGNIYAFPWIEELGNGKERIQAVDSMPWINVEWL 193 KN+ + + + A + I DG Y P A ++ +W Sbjct: 110 --PKNIIGGENIFDTAWKSTQYI---DGKYYGVP-----------TSAQTFALFVRKDWR 153 Query: 194 KKLGLEMPKTTDELKKVLIAFKTQDPNGNGQADEIPLSFINKPGAEDLAF---LFASFGL 250 +KLGLE PK ++ + AF DP+GNG+ D FI PG+ + +S+ Sbjct: 154 EKLGLEQPKNWQDISTLAKAFTFNDPDGNGKNDT--YGFI-LPGSTTRGYASWFISSYLW 210 Query: 251 GENPDHAVVSDDGKVIFTAAEEGYKNAISFINELYKEGLIDIEAYTQDWSTYLAKGKEQR 310 D + +GK I + E +SF+ + + + A + + + + Sbjct: 211 QAGGDFIRPAGEGKFIASLEEPAATETLSFMRGMVCDKTVQPGAINATTADAIPSFRSGQ 270 Query: 311 YGLYFS-------WDKANISGANDAYDVMPPLAGPDGEVNVTRTNGLGLGRGKMVVTSAN 363 G++FS +DK +A++V+PP GP G+ + + + + +A Sbjct: 271 SGMFFSGPYHIALFDK---DPGTEAFEVIPPPTGPKGQATLAEGTTVFMMKSSQKKAAAQ 327 Query: 364 KNLE 367 K +E Sbjct: 328 KFIE 331 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 40,789,638 Number of extensions: 1802893 Number of successful extensions: 2445 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 2439 Number of HSP's successfully gapped: 7 Length of query: 539 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 428 Effective length of database: 25,576,357 Effective search space: 10946680796 Effective search space used: 10946680796 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 118 (50.1 bits)