BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0003.p (498 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_sacC [G] COG1621 Beta-fructosidases (levanase/invertase) 478 e-134 BS_yveB [G] COG1621 Beta-fructosidases (levanase/invertase) 268 1e-71 SPCC191.11 [G] COG1621 Beta-fructosidases (levanase/invertase) 258 2e-68 CAC1773 [G] COG1621 Beta-fructosidases (levanase/invertase) 253 4e-67 SPAC8E11.01c [G] COG1621 Beta-fructosidases (levanase/invertase) 246 7e-65 >BS_sacC [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 478 bits (1230), Expect = e-134, Method: Compositional matrix adjust. Identities = 0/485 (0%), Positives = 0/485 (0%), Gaps = 14/485 (2%) Query: 7 QNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKDLVTWEE 66 + + Y + ++ G + + Sbjct: 32 AHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGP 91 Query: 67 LPVALFPDELGMIFSGSAVVDWNNTTGFFADKPG-LVAIFTHHLDMPEGQPPVQRQSLAY 125 + + + + + Sbjct: 92 SRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQ---RPT---NCFGKLG 145 Query: 126 SHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSPDLIHW 185 + + + + + ++ + Sbjct: 146 YSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQ 205 Query: 186 TFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQYFTGD 245 S + + L G + Sbjct: 206 NPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNG-AKPAGARNTLFSRFTR 264 Query: 246 FDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQTPAEEF 305 + G + +G D + +G + + Sbjct: 265 YSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHL 324 Query: 306 RGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQEVNAS-LAGLQLDAY 364 Sbjct: 325 384 Query: 365 EIAA--KLPRGMSA--GFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHERY-PGRH 419 Sbjct: 385 444 Query: 420 SAALQAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFAEHEETPFLSL 479 Sbjct: 445 504 Query: 480 EVYKL 484 Sbjct: 505 509 >BS_yveB [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 268 bits (686), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 0/490 (0%), Positives = 0/490 (0%), Gaps = 29/490 (5%) Query: 3 ASTKQNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKDLV 62 + + P E + H + S++ Sbjct: 37 DYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNND 96 Query: 63 TWEELPVAL--FPDELGMIFSGSAVVDWNNTTGFFADKPGLVAIFTHHLDMPEGQPPVQR 120 AL + S + + Sbjct: 97 KGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQ--VHWPQRPTNCF----GKLGYSWTD 150 Query: 121 QSLAYSHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSP 180 + A S + + + T+ +S Sbjct: 151 SAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSL 210 Query: 181 DLIHWTFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQ 240 + + ++GV GA T+ Sbjct: 211 LNRSFEVGLAE---VSQYEGVELLAYSCLGFGTLTGK--YLNGAKPAGARN-TLFSRFTR 264 Query: 241 YFTGDFDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEAD--GRRLFIGWMSNWMYAQ 298 Y V + R A + + + + Sbjct: 265 YSGEQTQKAVAAYVD--IARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESL 322 Query: 299 QTPAEE--FRGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQEVNASL 356 E Sbjct: 323 HLELSEDV--- 379 Query: 357 A-GLQLDAYEIAAKLPRGMSAGFKVRVGAGEETVTGID----AQTGELYVDRAASGLTGF 411 Sbjct: 380 439 Query: 412 HERYPGRHSAALQAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFAEH 471 Sbjct: 440 - 498 Query: 472 EETPFLSLEV 481 Sbjct: 499 508 >SPCC191.11 [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 258 bits (659), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 0/506 (0%), Positives = 0/506 (0%), Gaps = 44/506 (8%) Query: 10 RGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKDLVTWEELPV 69 S ++ + ++ H + + Sbjct: 83 IIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFG 142 Query: 70 ALFPDE-----LGMIFSGSAVVDWNNTTGFFAD----KPGLVAIFTHHLDMPEGQPPVQR 120 L + + G + + Sbjct: 143 KLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLH-----LADKHD 197 Query: 121 QSLAYSHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQT--VCLYH 178 + N + L + + + ++ + + Sbjct: 198 LPRIVTIQNPYSLLNRSFEVGLAEVS-QYEGVELLAYSCLGFGTLTGKYLNGAKPAGARN 256 Query: 179 SPDLIHWTFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSR 238 + ++ E + V Sbjct: 257 TLFSRFTRYSGEQTQK-AVAAYVDIARRHGLDPAQM-ALAFVRRQPFVASTLLGAT-TMD 313 Query: 239 TQYFTGDFDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQ 298 + + D Sbjct: 314 QLKTNIESLHLELSED-VLAE-- 370 Query: 299 QTPAEEFRGAMTLPRELYLESREGNIL-----LVQKPAQELEAARIPVLSLKDVTVQEVN 353 Sbjct: 371 - 429 Query: 354 ASLAGLQL---DAYEIAAKLPRGMSAGFKVRVGAGEETVTGID---AQTGELYVDRAASG 407 Sbjct: 430 489 Query: 408 LTGFHERYPGRHSAALQAPGEIHDLHIY--------VDRTSVEVFGNGGQAVIT-DLIFP 458 Sbjct: 490 549 Query: 459 KPESAGIAAFAEHEETPFLSLEVYKL 484 Sbjct: 550 575 >CAC1773 [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 253 bits (647), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 0/494 (0%), Positives = 0/494 (0%), Gaps = 36/494 (7%) Query: 3 ASTKQNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKDLV 62 + Q + + + +H + A + Sbjct: 38 YAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDK 97 Query: 63 TWEELPVAL--FPDELGMIFSGSAVVDWNNTTGFFADKPGLVAIFTHHLDMPEGQPPVQR 120 E D+ + P G Sbjct: 98 GIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV--HWPQRPTNCFG----KLGYSWTDS 151 Query: 121 QSLAYSHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSP 180 D +Y+ + + V + Y Sbjct: 152 APAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLL 211 Query: 181 DLIHWTFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQ 240 + +E T + + Sbjct: 212 NRSFEVGLAEV---SQYEGVELLAYSCLGFG--TLTGKYLNGAKPAGARNTL-FSRFTRY 265 Query: 241 YFTGDFDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADG--RRLFIGWMSNWMYAQ 298 D LD + A V + +N Sbjct: 266 SGEQTQKAVAAYV--DIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLH 323 Query: 299 QTPA--EEFRGAMTLPRELYLESREGNIL-LVQKPAQELEAARIPVLSLKDVTVQEVNAS 355 + Sbjct: 324 LELSED- 382 Query: 356 LAGLQLDAYEIAAKLP--RGMSAGFKVR--VGAGEETVTGIDAQTGELYVDRAASGLTGF 411 Sbjct: 383 - 441 Query: 412 HERYPGRHSAALQAPGEIHD----LHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAA 467 Sbjct: 442 ----- 496 Query: 468 FAEHEETPFLSLEV 481 Sbjct: 497 510 >SPAC8E11.01c [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 246 bits (628), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 20/342 (5%), Positives = 50/342 (14%), Gaps = 32/342 (9%) Query: 10 RGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKDLVTWEELPV 69 + H S + + + + G A + E Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 70 ALFP-DELGMIFSGSAVVDWNNTTGFFAD----------KPGLVAIFTHHLDMPEGQPPV 118 + GS + + L Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSL----- 118 Query: 119 QRQSLAYSHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQ--TVCL 176 ++ D + + D + + + Sbjct: 119 -KRLQTDYLDLYQVHWPQRPTNCF-GKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYI 176 Query: 177 YHSPDLIHWTFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEG 236 S + ++ + + + A Sbjct: 177 GVSNETAFGVMRYLHLAD-KHDLPRIVTIQNPYSLLNR-SFEVGLAEVSQYEGVELLAYS 234 Query: 237 SRTQYFTGDFDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMY 296 N P T+ + V + + Sbjct: 235 CLGFGTLTGKYLNGAKPAGARNTL--FSRFTRYSGEQTQKAVA--AYVDIARRHGLDPAQ 290 Query: 297 AQQTPAE-EFRGAMTLPREL-----YLESREGNILLVQKPAQ 332 + A TL ++ L + Sbjct: 291 MALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLA 332 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 42,755,975 Number of extensions: 2018951 Number of successful extensions: 4159 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 4106 Number of HSP's successfully gapped: 14 Length of query: 498 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 387 Effective length of database: 25,576,357 Effective search space: 9898050159 Effective search space used: 9898050159 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)