BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0001.p (1296 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_sacC [G] COG1621 Beta-fructosidases (levanase/invertase) 405 e-112 BS_yveB [G] COG1621 Beta-fructosidases (levanase/invertase) 218 7e-56 CAC1773 [G] COG1621 Beta-fructosidases (levanase/invertase) 203 2e-51 CAC1462 [G] COG1621 Beta-fructosidases (levanase/invertase) 188 5e-47 SPCC191.11 [G] COG1621 Beta-fructosidases (levanase/invertase) 174 8e-43 >BS_sacC [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 405 bits (1040), Expect = e-112, Method: Compositional matrix adjust. Identities = 0/501 (0%), Positives = 0/501 (0%), Gaps = 27/501 (5%) Query: 397 SYYNETFRPRYHYSPVRGSASDPNGLVYFEGEYHLFHQ--------DGGTWAHAVSKDML 448 S + + Y + + Sbjct: 28 SEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASK 87 Query: 449 NWKRLPIALPWNDYGHIWSGXXXXXXXXXSGLFTDSGGKGLIAYYTSYNPDGPNGNQRIG 508 S + G Sbjct: 88 VSGPSRNNDKGIRPDQALDRKNIREALHDSL-KRLQTDYLDLYQVHW----PQRPTNCFG 142 Query: 509 LAYSKDKGRTWEYAKDRPIVIENPGKNGEDPGGWDFRDPKVVRDDGNNRWVMVVSGGDHI 568 + + + + + ++ Sbjct: 143 KLGYSWTDSAPAVSLLDT-LDALAEYQ-------RAGKIRYIGVSNETAFGVMRYLHLAD 194 Query: 569 RFFTSTNLLDWTLTDNWGYGDYVRGGVWECPDLFPLTVQGT-SQKKWVLMISTGANPATG 627 + + V + L GA PA Sbjct: 195 KHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGA 254 Query: 628 GSDSEYFVGSLTAEGKFVNDNPAGKVLRTDFGKEYYASMSFSDMP--DGRRVMLAWMSNW 685 + + E + A + G + +L + Sbjct: 255 RNTLFSRFTRYSGE-QTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMD 313 Query: 686 DYPFAFPTSGWKGELTVPREVTLVMTPEGLRLAQSPVKEIEQLRSRLFTAADKRVNSSSP 745 Sbjct: 314 373 Query: 746 NLLKGLVAGAYEIEAELEIPDGSTATEFGFNVREGADQKTVVGYKVGESQLFVDRSASGV 805 Sbjct: 374 - 432 Query: 806 TDFSSLFSTRHEAA-MTAENKRIKLRILVDESSVEVFGNGGKAVFSEVIFPDPASRAMSF 864 Sbjct: 433 492 Query: 865 YSQGGMVKVISLKVNKLGPVW 885 Sbjct: 493 513 Score = 100 bits (249), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 0/170 (0%), Positives = 0/170 (0%), Gaps = 12/170 (7%) Query: 975 KVYGG-EFHTNLSGWTQDLSMASWIATGDGIRGKYSSDANHMAQEQAGNFTYEADMKL-- 1031 Sbjct: 511 -- 568 Query: 1032 GESGGAGSLLFRASGDGRSGYYLNLDPNMKSVRLFYKLDGRFEERQG--LAKYPAFILPG 1089 Sbjct: 569 ----- 623 Query: 1090 QTYNIKIQAEGPRIIVYMGGRQIMDLKDGTFAEGHFGLHVFGGEASFQNV 1139 Sbjct: 624 673 Score = 88.6 bits (218), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 0/161 (0%), Positives = 0/161 (0%), Gaps = 7/161 (4%) Query: 71 ITNLTGWQVKGKGEMKNTAEGLLLTSDPQQNVMAVSETAAEDFLYEADVMLKEL--KADT 128 Sbjct: 519 --- 575 Query: 129 SLIFRSDDDGKNSYMLQLAPGSGVIRLKDAAGGAGKLYEERQVSLKEGEIYHLKVKAEGT 188 Sbjct: 576 635 Query: 189 SLKVYWGNQYKPVIEIQDSTYRSGRLGLHVRDGSALFQNIT 229 Sbjct: 636 -- 674 Score = 51.6 bits (122), Expect = 9e-06, Method: Compositional matrix adjust. Identities = 0/222 (0%), Positives = 0/222 (0%), Gaps = 21/222 (9%) Query: 181 LKVKAEGTSLKVYWGNQYKPVIEIQDSTYRSGR-LGLHVRDGSALFQNITVSDLKGNLG- 238 Sbjct: 457 - 515 Query: 239 -PVLMN-------KGQWQPDLRGQKGTSANGSKAQRIYSRQAADLVYEGTIXXXXXX--- 287 Sbjct: 516 -- 573 Query: 288 XXXXXXXXXXDGVRGYEAVLAREGDQVGV-KLAKADGTVVASSEHLYPSRTGAKHHVEIK 346 Sbjct: 574 -- 631 Query: 347 AKGSRIQVFLDGYTPAAIDIQDTAYSTGHAGLVVKTGNAYFQ 388 Sbjct: 632 -- 671 >BS_yveB [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 218 bits (554), Expect = 7e-56, Method: Compositional matrix adjust. Identities = 0/501 (0%), Positives = 0/501 (0%), Gaps = 48/501 (9%) Query: 403 FRPRYHYSPVRGSASDPNGLVYFEGEYHLFHQ--------DGGTWAHAVSKDMLNWKRLP 454 + + P E + + S++ R Sbjct: 43 GINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD 102 Query: 455 IALP--WNDYGHIWSGXXXXXXXXXSGLFTDSGGKGLIAYYTSYNPDGPNGNQRIGLAYS 512 AL S A S Sbjct: 103 QALDRKNIREALHDSLKRLQTDYLDLYQ----VHWPQRPTNCF--GKLGYSWTDSAPAVS 156 Query: 513 KDKGRTWEYAKDRPIVIENPGKNGEDPGGWDFRDPKVVRDDGNNRWVMVVSGGDHIRFFT 572 R V + + I+ Sbjct: 157 LLDTLDALAEYQRA-GKIRYIGV-------SNETAFGVMRYLHLADKHDLPRIVTIQNPY 208 Query: 573 STNLLDWTLTDNWGYGDYVRGGVWECPDLFPLTV-QGTSQKKWVLMISTGANPATGGSDS 631 S + + GV GA + Sbjct: 209 SLLNRSFEVGL--AEVS-QYEGVELLAYSCLGFGTLTGK--YLNGAKPAGARNTLFSRFT 263 Query: 632 EYFVGSLTAEGKFVNDNPAGKVLRTDFGKEYYASMSFSDM----PDGRRVMLAWMSNWDY 687 Y + A + + + + + Sbjct: 264 RYSGEQTQKA-VAAYVDIARRHG--LDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIE 320 Query: 688 PFAFPT--SGWKGELTVPREVTLVMTPEGLRLAQSPVKEIEQLRSRLFTAADKRVNSSSP 745 Sbjct: 321 SLHLELSE 380 Query: 746 NLLKGLVAGAYEIEAELEIPDGSTATEFGFNVREGADQK--TVVGYKVGESQLFVDRSAS 803 Sbjct: 381 ------ 434 Query: 804 GVTDFSSLFSTRHEAAMTAENKRIKLRILVDESSVEVFGNGGKAVFSEVIFPDPASRAMS 863 Sbjct: 435 - 493 Query: 864 FYSQGGMVKVISLKVNKLGPV 884 Sbjct: 494 514 >CAC1773 [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 203 bits (516), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 0/516 (0%), Positives = 0/516 (0%), Gaps = 55/516 (10%) Query: 386 YFQETYLTDADSYYNETFRPRYHYSPVRGSASDPNGLVYFEGEYH---LFHQD-----GG 437 + D + + + Sbjct: 27 NSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIAS 86 Query: 438 TWAHAVSKDMLNWKRLPIALP--WNDYGHIWSGXXXXXXXXXSGLFTDSGGKGLIAYYTS 495 + + + + H + Sbjct: 87 KVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV-----HWPQRPTNCF 141 Query: 496 YNPDGPNGNQRIGLAYSKDKGRTWEYAKDRPIVIENPGKNGEDPGGWDFRDPKVVRDDGN 555 G + D + + G + V+R Sbjct: 142 -GKLGYSWTDSAPAVSLLDTLDALAEYQRAGKI--------RYIGVSNETAFGVMRYLHL 192 Query: 556 NRWVMVVSGGDHIRFFTSTNLLDWTLTDNWGYGDYVRG--GVWECPDLFPLTVQGTSQKK 613 + ++ N + + T + Sbjct: 193 ADKHDLPRIVTIQNPYSLLNRSFEVGL-----AEVSQYEGVELLAYSCL-GFGTLTGKYL 246 Query: 614 WVLMISTGANPATGGSDSEYFVGSLTAEGKFVNDNPAGKVLRTDFGKEYYASMSFSDMPD 673 + N + A D L D + A + Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAV-AAYVDIARRHGL--DPAQMALAFVRRQPFVA 303 Query: 674 G----RRVMLAWMSNWDYPFAFPT--SGWKGELTVPREVTLVMTPEG-LRLAQSPVKEIE 726 M +N + + Sbjct: 304 STLLGATTMDQLKTNIESLHLELSED 363 Query: 727 QLRSRLFTAADKRVNSSSPNLLKGLVAG-AYEIEAELEIPDGSTATEFGFNVREGADQK- 784 Sbjct: 364 ------- 416 Query: 785 -TVVGYKVGESQLFVDRSASGVTDFSSLFSTRHEAAMTAENKRIKLRILVDESSVEVFGN 843 Sbjct: 417 - 475 Query: 844 GGKAVFSEVIFPDPASRAMSFYSQGG--MVKVISLK 877 Sbjct: 476 511 >CAC1462 [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 188 bits (477), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 21/310 (6%), Positives = 45/310 (14%), Gaps = 34/310 (10%) Query: 376 AGLVVKTGNAYFQ-ETYLTDADSYYNETFRPRYHYSPVRGSASDPNGLVYFEGEYHLFHQ 434 + G F + DA + + + V P Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 435 DGG--------TWAHAVSKDMLNWKRLPIALPWNDYGHIWSGXXXXXXXXXSGLFTD-SG 485 A VS N + D +I + Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV 131 Query: 486 GKGLIAYYTSYNPDGPNGNQRIGLAYSKDKGRTW-EYAKDRPIVIENPGKNGEDPGGWDF 544 A S + + N + Sbjct: 132 HWPQRPTNCFGKLGY--SWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN-------ET 182 Query: 545 RDPKVVRDDGNNRWVMVVSGGDHIRFFTSTNLLDWTLTDNWGY-GDYVRGGVWECPDLFP 603 + ++ + + + L + Y G + Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 604 LTVQGTSQKKWVLMISTGA-----NPATGGSDSEYFVG-----SLTAEGKFVNDNPAGKV 653 ++ T + + Y A+ Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPF- 301 Query: 654 LRTDFGKEYY 663 Sbjct: 302 --VASTLLGA 309 Score = 150 bits (378), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 0/256 (0%), Positives = 0/256 (0%), Gaps = 21/256 (8%) Query: 645 VNDNPAGKVLRTDFGKEYYASMSFSD---------------MPDGRRVMLAWMSNWDYPF 689 Sbjct: 552 611 Query: 690 AFPTSGWKGELTVPREVTLVMTPEGLRLAQSPVKEIEQLRSRLFTAADKRVNSSSPNLLK 749 Sbjct: 612 671 Query: 750 GLVAGAYEIEAELEIPDGSTATEFGFNVREGADQKTVVGYKVGESQLFVDRSASGVTDFS 809 Sbjct: 672 -- 729 Query: 810 SLFSTRHEAAMTAENK-RIKLRILVDESSVEVFG---NGGKAVFSEVIFPDPASRAMSFY 865 Sbjct: 730 789 Query: 866 SQGGMVKVISLKVNKL 881 Sbjct: 790 805 Score = 57.0 bits (136), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 0/139 (0%), Positives = 0/139 (0%), Gaps = 17/139 (12%) Query: 1010 SDANHMAQEQAGNFTYEA------DMKLGESGGAGSLLFRASGDGRSGYYLNLDPNMKSV 1063 Sbjct: 1008 - 1066 Query: 1064 RLFYKLDGR---FEERQGLAKYPAFILPGQTYNIKIQAEGPRIIVYMGGRQIMDLKDGTF 1120 Sbjct: 1067 ------- 1119 Query: 1121 AEGHFGLHVFGGEASFQNV 1139 Sbjct: 1120 1138 >SPCC191.11 [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 174 bits (441), Expect = 8e-43, Method: Compositional matrix adjust. Identities = 0/524 (0%), Positives = 0/524 (0%), Gaps = 53/524 (10%) Query: 398 YYNETFRPRYHYSPVRGSASDPNGLVYFEGEYHLFHQDGGT--------WAHAVSKDMLN 449 E S + ++ + Sbjct: 77 GSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQR 136 Query: 450 WKRLPIALPWNDYGH-----IWSGXXXXXXXXXSGLFTDSG--GKGLIAYYTSYNPDGPN 502 L ++ + +G G + Sbjct: 137 PTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMR----YLHL 192 Query: 503 G-NQRIGLAYSKDKGRTWEYAKDRPIVIENPGKNGEDPGGWDFRDPKVVRDDGNNRWVMV 561 + + + + + + Sbjct: 193 ADKHDLPRIVTIQNPYSLLNRSFEVGL---------AEVSQYEGVELLAYSCLGFGTLTG 243 Query: 562 VSGGDH--IRFFTSTNLLDWTLTDNWGYGDYVRGGVWECPDLFPLTVQGTSQKKWVLMIS 619 + + V Q Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVA-AYVDIARRHGLDPAQMALAFVRRQPFV 302 Query: 620 TGANPATGGSDSEYFVGSLTAEGKFVNDNPAGKVLRTDFGKEYYAS-MSFSDMPDGRRVM 678 D + D + Y + Sbjct: 303 ASTLLGATTMDQLKTNIESLHL-ELSEDV--LAEIEAVHQVYTYPAP 359 Query: 679 LAWMSNWDYPFAFPTSGWKGELTVPREVTLVMTPEG-----LRLAQSPVKEIEQLRSR-L 732 Sbjct: 360 - 418 Query: 733 FTAA---DKRVNSSSPNLLKGLVAGAYEIEAELEIPDGSTA-TEFGFNVREGAD----QK 784 Sbjct: 419 478 Query: 785 TVVGYKVGESQLFVDRSASGVTDFSSLFSTRHEAAMTAENKRIKLRILVDESSVEVFGNG 844 Sbjct: 479 - 537 Query: 845 GKAVFS-EVIFPDPASRAMSFYSQGGMVKVISLKVNKLGPVWNS 887 Sbjct: 538 581 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.132 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 101,288,417 Number of extensions: 4551941 Number of successful extensions: 6415 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 6363 Number of HSP's successfully gapped: 17 Length of query: 1296 Length of database: 38,378,431 Length adjustment: 118 Effective length of query: 1178 Effective length of database: 24,769,019 Effective search space: 29177904382 Effective search space used: 29177904382 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 122 (51.6 bits)