BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0532.0008.p
         (284 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CAC0748 [C] COG0778 Nitroreductase                                     82   1e-15
MTH933 [C] COG0778 Nitroreductase                                      53   5e-07

>CAC0748 [C] COG0778 Nitroreductase
          Length = 346

 Score = 81.6 bits (200), Expect = 1e-15,   Method: Compositional matrix adjust.
 Identities = 12/250 (4%), Positives = 39/250 (15%), Gaps = 28/250 (11%)

Query: 7   LPLEETIKTRSSVRTYSRESIEQGTKEQIQWFIGTLGNPFSAEVHFKLLQTEQAA--NAR 64
           +       +   V T    ++  G +         L           L+   +      R
Sbjct: 1   MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYA--VAQGINLIDVAEMYPVPPR 58

Query: 65  KLGTYGVIKGAKDYIGASVTNGALALEALGYEFEHLVLYATSLGLGTCWMGGTFNRSEFA 124
                  +         +       L                +             +   
Sbjct: 59  P--ETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREA--L 114

Query: 125 KAMDVREGQLFPVISPIGYAAGKRRLTENIVRKVAKSDQRMPWETLFFRESFNNPLTPAE 184
                R    +  +  + +          +      S   +              L    
Sbjct: 115 HDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT---------LDALA 165

Query: 185 AGDYAYPLEM-TRLAPSASNKQPWRIIRDADGQTYHFYEYKARGYSDALGYDIQKVDLGI 243
               A  +        +A     +              +         +      ++   
Sbjct: 166 EYQRAGKIRYIGVSNETAFGVMRYLH----------LADKHDLPRIVTIQNPYSLLNRSF 215

Query: 244 AACHFQLAAE 253
                +++  
Sbjct: 216 EVGLAEVSQY 225


>MTH933 [C] COG0778 Nitroreductase
          Length = 346

 Score = 53.1 bits (126), Expect = 5e-07,   Method: Compositional matrix adjust.
 Identities = 9/190 (4%), Positives = 24/190 (12%), Gaps = 28/190 (14%)

Query: 75  AKDYIGASVTNGALALEALGYEFEHLVLYATSLGLGTCWMGGTFNRSEFAKAMDVREGQL 134
                       A             V   +             +R    +A+       
Sbjct: 63  GLTET-YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALH------ 115

Query: 135 FPVISPIGYAAGKRRLTENIVRKVAKSDQRMPWETLFFRESFNNPLTPAEAGDYAYPLEM 194
             +              +       +              +    L  +           
Sbjct: 116 DSLKRLQTDYL------DLYQVHWPQ-----------RPTNCFGKLGYSWTDSAP--AVS 156

Query: 195 TRLAPSASNKQPWRIIRDADGQTYHFYEYKARGYSDALGYDIQKVDLGIAACHFQLAAED 254
                 A  +              +   +    Y            +      + L    
Sbjct: 157 LLDTLDALAEYQRAGK-IRYIGVSNETAFGVMRYLHLADK-HDLPRIVTIQNPYSLLNRS 214

Query: 255 KNLPGAFRVL 264
             +  A    
Sbjct: 215 FEVGLAEVSQ 224


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.319    0.135    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 21,687,483
Number of extensions: 858409
Number of successful extensions: 2307
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 2305
Number of HSP's successfully gapped: 2
Length of query: 284
Length of database: 38,378,431
Length adjustment: 105
Effective length of query: 179
Effective length of database: 26,268,361
Effective search space: 4702036619
Effective search space used: 4702036619
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 115 (48.9 bits)