BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0532.0002.p (294 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA2230 [V] COG1680 Beta-lactamase class C and other penicillin b... 124 2e-28 BH0715 [V] COG1680 Beta-lactamase class C and other penicillin b... 100 2e-21 BS_pbpE [V] COG1680 Beta-lactamase class C and other penicillin ... 96 5e-20 alr0153 [V] COG1680 Beta-lactamase class C and other penicillin ... 94 3e-19 all2656 [V] COG1680 Beta-lactamase class C and other penicillin ... 88 2e-17 >SA2230 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 124 bits (310), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 15/190 (7%), Positives = 37/190 (19%), Gaps = 1/190 (0%) Query: 48 MVETIMDKTATPGVAIVSSKQGKAEF-KVYGYADVDQKKQVTEESLFELGSTTKAFTALA 106 A I SS AE + + + L + Sbjct: 46 TALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQW 105 Query: 107 IILLQEEGALAYSDYISDYLPWFAPTYKGEKTKISINQLLVHTSGIPSWSIRLIPEGSTE 166 + E S + + + + + P + Sbjct: 106 LPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAY 165 Query: 167 EMLGTTIHKISGITLDTYPGTEHQYATVNYDILAMIIEQVTGISYQDYVTQHILVPLGMT 226 + + I TY + + + A + + + M Sbjct: 166 YDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQML 225 Query: 227 DSYFSSGQEK 236 + + + Sbjct: 226 EEQYPLQENG 235 >BH0715 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 100 bits (249), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 14/182 (7%), Positives = 34/182 (18%), Gaps = 4/182 (2%) Query: 47 NMVETIMDKTATPGVAIVSSKQGKAEF-KVYGYADVDQKKQVTEESLFELGSTTKAFTAL 105 + + + + A+ + + + + AL Sbjct: 36 ADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAE-ARSALPDCQFVEADIRNWQPVQAL 94 Query: 106 AIILLQEEGALAYSDYISDYLPWFAPTYKGEKTKISINQLLVHTSGIPSWS-IRLIPEGS 164 +I Y +G + L T + Sbjct: 95 DLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDR 154 Query: 165 TEE-MLGTTIHKISGITLDTYPGTEHQYATVNYDILAMIIEQVTGISYQDYVTQHILVPL 223 E + G + II+ VT + ++ Sbjct: 155 GREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQ 214 Query: 224 GM 225 + Sbjct: 215 QL 216 >BS_pbpE [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 96.3 bits (238), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 6/191 (3%), Positives = 23/191 (12%), Gaps = 18/191 (9%) Query: 46 QNMVETIMDKTATPGVAIVSSKQGKAEFKVYGYADVDQKKQVTEESLFELGSTTKAFTAL 105 + + G + Q + G + Sbjct: 10 LHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIA 69 Query: 106 AIILLQEEGALAYSDYISDYLPWFAPTYKGEKTKISINQLLVHTSGIP--------SWSI 157 + +D + + L Sbjct: 70 EARSALPDCQFVEADIRNWQPVQAL--DLI----FANASLQWLPDHYELFPHLVSLLNPQ 123 Query: 158 RLIPEGSTEEMLGTTIHKISGITLDTYPGTEHQYATVNYDILAMIIEQVTGISYQDYVTQ 217 ++ + + + + + + Sbjct: 124 GVL--AVQMPDNWLEPTH--VLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIW 179 Query: 218 HILVPLGMTDS 228 M Sbjct: 180 RTTYYHQMPSH 190 >alr0153 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 93.6 bits (231), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 13/185 (7%), Positives = 34/185 (18%), Gaps = 15/185 (8%) Query: 54 DKTATPGVAIVSSKQGKAEFKV-YGYADVDQKKQVTEESLFELGSTTKAFTALAIILLQE 112 + A +A V + + + G + Q + G + Sbjct: 17 SRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSALP 76 Query: 113 EGALAYSDYISDYLPWFAPTYKGEKTKISINQLLVHTSGIPSW-------SIRLIPEGST 165 + +D + + Q L + L + Sbjct: 77 DCQFVEADIRNWQPVQA-LDL---IFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPD 132 Query: 166 EEMLGTTIHKISGITLDTYPGTEHQYATVNYDILAMIIEQVTGISYQDYVTQHILVPLGM 225 + T + + + + A D + Sbjct: 133 NWLEPTHVLMRE---VAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPS 189 Query: 226 TDSYF 230 + Sbjct: 190 HQAII 194 >all2656 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 0/214 (0%), Positives = 0/214 (0%), Gaps = 20/214 (9%) Query: 49 VETIMDKTA--TPG--VAIVSSKQGKAEFKVYGYADVDQKKQVTEESLFELGSTTKAFTA 104 ++ A+++ + + + D + ++L + + Sbjct: 49 LQQRWPAARITGIDSSPAMIAEARSA--LPDCQFVEADIRNWQPVQALDLIFANASLQWL 106 Query: 105 LAIILLQEEGALAYSDYISDYLPWFAPTYKGEKTKISINQLLVHTSGIPSWSIRLIPEGS 164 L + + + + + + + Sbjct: 107 PDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYY 166 Query: 165 TE----------EMLGTTIHKISGITLDTYPGTEHQYATVNYDILAMIIEQVTGISYQDY 214 + + + EQ + Sbjct: 167 DILSEAGCEVDIWRT---TYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQ 223 Query: 215 VTQHILVPLGMTDSYFSSGQEKKPDQLTQGYRVF 248 + + + Sbjct: 224 MLEEQYPLQENGQILLAF- 256 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,031,908 Number of extensions: 809161 Number of successful extensions: 1333 Number of sequences better than 1.0e-05: 54 Number of HSP's gapped: 1291 Number of HSP's successfully gapped: 54 Length of query: 294 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 188 Effective length of database: 26,153,027 Effective search space: 4916769076 Effective search space used: 4916769076 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)