BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0531.0003.p (572 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VC1878 [V] COG1132 ABC-type multidrug transport system, ATPase a... 214 4e-55 YPO1395 [V] COG1132 ABC-type multidrug transport system, ATPase ... 203 8e-52 all0640 [V] COG1132 ABC-type multidrug transport system, ATPase ... 203 8e-52 BS_ygaD [V] COG1132 ABC-type multidrug transport system, ATPase ... 201 2e-51 STM0984 [V] COG1132 ABC-type multidrug transport system, ATPase ... 197 3e-50 >VC1878 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 582 Score = 214 bits (544), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 155/571 (27%), Positives = 284/571 (49%), Gaps = 26/571 (4%) Query: 9 VREHKVLLTVTLLCSILTSAAGVLIALILQKVIDAAL-QGDRQLFREIXXXXXXXXXXXG 67 +R +K L V+ + ++ +AA + +L+ ++D + R + G Sbjct: 20 IRLYKAGLVVSTIALVINAAADTYMISLLKPLLDEGFGNAESNFLRILPFMILGLMFVRG 79 Query: 68 VFGFIYSLCSKALIRNVTISLRERVFRGVFGQKPETFSATNTADYLSALTNDIKLLEDNY 127 + GF S C + NV + +R R+F F +T LS +T D + + Sbjct: 80 LSGFASSYCLSWVSGNVVMQMRRRLFNHFMHMPVRFFDQESTGGLLSRITYDSEQVAGAT 139 Query: 128 IQPMLQVLQNVVVFAVSLGILLYLSPLVTVILIGCM-VLMFAIPSLFGKALQHKQSEVSS 186 + ++ +++ L ++ + S ++++LI V+ FAI S K + + + Sbjct: 140 SRALVSIVREGASIIGLLTLMFWNSWQLSLVLIVVAPVVAFAI-SFVSKRFRKISRNMQT 198 Query: 187 QMSAFTASLKDLLSGYEVITSYAMGKY-------AGDTFKQQNHRAAGVRFAADRLFAFN 239 M T+S + +L G++V+ SY + ++ +QQ + + AD Sbjct: 199 AMGHVTSSAEQMLKGHKVVLSYGGQEVERKRFDKVSNSMRQQTMKLVSAQSIAD------ 252 Query: 240 ESVSQTLAILTQFAVVFIAAYLIITGRLSAGSLVALVQLSGGFVGPMLAIMENLPKIQ-G 298 V Q +A L FAV+F+A+ I L+ G+ + G + P+ A+ + Q G Sbjct: 253 -PVIQMIASLALFAVLFLASVDSIRAELTPGTFTVVFSAMFGLMRPLKALTSVTSEFQRG 311 Query: 299 IKPVRERIDWLTGEKQEPDAEGLPPVFNSTLEARHLQFSYK-PGNPVIQDISLSLEKGKK 357 + + + E + + + N ++ + + F+Y+ P + +S S+ +GK Sbjct: 312 MAACQTLFGLMDLETERDNGKYEAERVNGEVDVKDVTFTYQGKEKPALSHVSFSIPQGKT 371 Query: 358 YALVGPSGCGKSTLVKLLTGYYDRFDGSIELDGTDVRRLDREKLQQMVSTIHQNVYMFDS 417 ALVG SG GKST+ L T +YD GSI LDG DVR L++ + + QNV++F+ Sbjct: 372 VALVGRSGSGKSTIANLFTRFYDVDSGSICLDGHDVRDYKLTNLRRHFALVSQNVHLFND 431 Query: 418 DIRHNICL--HEEFSEQMLDTALHTSGIHKFLQNTPDGLLSPVGENGLQLSGGQRQRIAV 475 I +NI E++ + ++ A + +F++N P GL + +GENG LSGGQRQR+A+ Sbjct: 432 TIANNIAYAAEGEYTREQIEQAARQAHAMEFIENMPQGLDTVIGENGTSLSGGQRQRVAI 491 Query: 476 ARALIRNKPILVLDEGTSAIDMQTAYEIESRL--LKINELTLITITHNMNEELLGLYDQI 533 ARAL+R+ P+L+LDE TSA+D ++ I++ L L+ N+ T++ I H ++ + D+I Sbjct: 492 ARALLRDAPVLILDEATSALDTESERAIQAALDELQKNK-TVLVIAHRLST--IEQADEI 548 Query: 534 IYMEDGQIAETGSLEELLKQGGRFRSFYTLE 564 + +++G+I E G +LL Q G + + ++ Sbjct: 549 LVVDEGEIIERGRHADLLAQDGAYAQLHRIQ 579 >YPO1395 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 582 Score = 203 bits (516), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 148/501 (29%), Positives = 257/501 (51%), Gaps = 17/501 (3%) Query: 67 GVFGFIYSLCSKALIRNVTISLRERVFRGVFGQKPETFSATNTADYLSALTNDIKLLEDN 126 GV GF+ S C + V + +R R+F + G F +T LS +T D + + + Sbjct: 79 GVTGFVSSYCISWVSGKVVMHIRRRLFSHMMGMPVSFFDQQSTGTLLSRITYDSEQVAAS 138 Query: 127 YIQPMLQVLQNVVVFAVSLGILLYLSPLVTVILIGCMVLMFAIPSLFGKALQHKQSEVSS 186 ++ V++ ++ Y S +++ILI ++ L K ++ + + Sbjct: 139 SSSALVTVVREGASIIGLFIMMFYYSWQLSLILIVIAPIVSISIRLVSKRFRNISKNMQN 198 Query: 187 QMSAFTASLKDLLSGYEVITSYAMGKYAGDTFKQQNHRAAGVRFAADRLFAFNESVSQTL 246 M T S + +L G++ + + K + F ++R +R +L + + + Sbjct: 199 TMGEVTTSAEQMLKGHKEVLIFGGQKVETERFDAVSNR---MRQQGMKLVSASSISDPII 255 Query: 247 AILTQFA---VVFIAAYLIITGRLSAGSLVALVQLSGGFVGPMLAIMENLPKIQ-GIKPV 302 ++ FA V++ A++ + L+AG++ + + P+ ++ + Q G+ Sbjct: 256 QLIASFALALVLYAASFPSVMETLTAGTITVVFSAMIALMRPLKSLTNVNTQFQRGMAAC 315 Query: 303 RERIDWLTGEKQEPDAEGLPPVFNSTLEARHLQFSYKPGN--PVIQDISLSLEKGKKYAL 360 + L E+++ + + +E RH+ F Y PG P + DI++ LE GK AL Sbjct: 316 QTLFSILDMEQEKDEGKLEVERAKGDIEFRHVTF-YYPGKDTPALNDINIHLEAGKTVAL 374 Query: 361 VGPSGCGKSTLVKLLTGYYDRFDGSIELDGTDVRRLDREKLQQMVSTIHQNVYMFDSDIR 420 VG SG GKST+ LLT +YD +GSI LDG D+R L+ V+ + QNV++F+ + Sbjct: 375 VGRSGSGKSTIANLLTRFYDVSEGSILLDGHDLRDYRLGALRNQVALVSQNVHLFNDTVA 434 Query: 421 HNICL--HEEFSEQMLDTALHTSGIHKFLQNTPDGLLSPVGENGLQLSGGQRQRIAVARA 478 +NI +E++S ++ A + F+ GL + +GENG+ LSGGQRQRIA+ARA Sbjct: 435 NNIAYARNEQYSRAEIEEAARMAYAMDFINKMEHGLDTVIGENGIMLSGGQRQRIAIARA 494 Query: 479 LIRNKPILVLDEGTSAIDMQTAYEIESRL--LKINELTLITITHNMNEELLGLYDQIIYM 536 L+RN PIL+LDE TSA+D ++ I++ L L+ N +L+ I H ++ + D+I+ + Sbjct: 495 LLRNCPILILDEATSALDTESERAIQAALDELQKNRTSLV-IAHRLST--IEKADEIVVI 551 Query: 537 EDGQIAETGSLEELLKQGGRF 557 EDG+I E G ELL Q G + Sbjct: 552 EDGRIVERGVHAELLVQQGVY 572 >all0640 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 546 Score = 203 bits (516), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 151/513 (29%), Positives = 261/513 (50%), Gaps = 30/513 (5%) Query: 68 VFGFIYSL----CSKALIRNVTISLRERVFRGVFGQKPETFSATNTADYLSALTNDI--- 120 V GF S+ SKA +R + LR++V+ + F D LT DI Sbjct: 42 VRGFFQSVQDIYMSKAALR-IAFYLRKQVYGHLQKLDLSYFETAKAGDLSYRLTEDIDRI 100 Query: 121 -----KLLEDNYIQPMLQVLQNVVVFAVSLGILLYLSPLVTVILIGCMVLMFAIPSLFGK 175 KL D +I +LQ++ + ++ + L L L+ ++ +G ++ FG+ Sbjct: 101 GEVVNKLFHD-FIPCVLQLIA-IPIYMIYLNWQLTLATVIVAPFMGILI------GWFGE 152 Query: 176 ALQHKQSEVSSQMSAFTASLKDLLSGYEVITSYAMGKYAGDTFKQQNHRAAGVRFAADRL 235 L+ +++S +A L ++ SG +I ++A Y F ++ R+ +++A+RL Sbjct: 153 RLRKYSLRSQNRVSDLSAILTEVFSGIRLIRAFAAENYEVTRFSREAERSFQAKYSAERL 212 Query: 236 FAFNESVSQTLAILTQFAVVFIAAYLIITGRLSAGSLVALVQLSGGFVGPMLAIMENLPK 295 A + L L+ +++ + A+ I L+ G + + +G + P+ + N + Sbjct: 213 KAVQIPIVGFLEALSALSLLLVGAWQIQQNNLTVGEFFSYLAAAGLLIDPIGHVTNNYNE 272 Query: 296 IQGIKPVRERIDWLTGEK----QEPDAEGLPPVFNSTLEARHLQFSYKPGNPVIQDISLS 351 + + +RI L + ++P+A LPPV +E + F+YKPG V++DI+L Sbjct: 273 FKQGEASVDRIFELMAIQPTVLEKPNAVTLPPV-KGKVEYSGVTFAYKPGETVLKDINLL 331 Query: 352 LEKGKKYALVGPSGCGKSTLVKLLTGYYDRFDGSIELDGTDVRRLDREKLQQMVSTIHQN 411 G+ A VG SG GK+T V LL +YD G I +DG D+R + + L++ + + Q Sbjct: 332 AMPGEAIAFVGASGAGKTTFVNLLPRFYDPSAGQILIDGVDIRDVRLQSLRRQIGIVPQE 391 Query: 412 VYMFDSDIRHNICLHEE-FSEQMLDTALHTSGIHKFLQNTPDGLLSPVGENGLQLSGGQR 470 MF + NI ++ F + + A + H+F+ P+G + VGE G+ LSGGQR Sbjct: 392 TIMFSGTVAQNIAFGQDIFDLEAVAAAAKIANAHQFISQLPEGYNTWVGERGVNLSGGQR 451 Query: 471 QRIAVARALIRNKPILVLDEGTSAIDMQTAYEIESRLLKI-NELTLITITHNMNEELLGL 529 QRIA+ARA++ N IL+LDE TSA+D ++ ++ L ++ T+ I H ++ + Sbjct: 452 QRIAIARAVLLNPQILILDEATSALDSESEALVQEALERLMQNRTVFIIAHRLST--VRR 509 Query: 530 YDQIIYMEDGQIAETGSLEELLKQGGRFRSFYT 562 D+I+ +E GQI E+G+ EELL R+ FYT Sbjct: 510 CDRILVLERGQIVESGTHEELLALENRYARFYT 542 >BS_ygaD [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 165 Score = 201 bits (512), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 0/580 (0%), Positives = 0/580 (0%), Gaps = 24/580 (4%) Query: 4 VMK---QAVREHKVLLTVTLLCSILTSAAGVLIALILQKVIDAALQG-----DR--QLFR 53 + +A + + + + + Sbjct: 12 QVGQALKARGATVTTAESCTGGWVAKVITDIAGSSAWFERGFVTYSNEAKAQMIGVREET 71 Query: 54 EIXXXXXXXXXXXGVFGFIYSLCSKALIRNVTISLRERVFRGVFGQKPETFSATNTADYL 113 + +++ F+ Sbjct: 72 LAQHGAVSEPVVVEMAIGALKAARADYAVSISGIAGPDGGSEEKPVGTVWFAFATARGEG 131 Query: 114 SALTNDIKLLEDNYIQPMLQVLQNVVVFAVSLGILLYLSPLVTVILIGCMVLM-FAIPSL 172 Sbjct: 132 191 Query: 173 FGKALQHKQSEVSSQMSAFTASLKDLLSGYEVITSYAMGKYAGDTFKQQNHRAAGVRFAA 232 Sbjct: 192 - 250 Query: 233 DRLFAFNESVSQTLAILTQFAVVFIAAYLIITGRLSAGSLVALVQLSGGFVGPMLAIMEN 292 Sbjct: 251 310 Query: 293 ----LPKIQGIKPVRERIDWLTGEKQEPDAEGLPPVFNSTLEARHLQFSY-KPGNPVIQD 347 Sbjct: 311 - 369 Query: 348 ISLSLEKGKKYALVGPSGCGKSTLVKLLTGYYDRFDGSIELDGTDVRRLDREKLQQMVST 407 Sbjct: 370 429 Query: 408 IHQNVYMFDSDIRHNICLHEEFS--EQMLDTALHTSGIHKFLQNTPDGLLSPVGENGLQL 465 Sbjct: 430 - 488 Query: 466 SGGQRQRIAVARALIRNKPILVLDEGTSAIDMQTAYEIESRLLKI-NELTLITITHNMNE 524 Sbjct: 489 548 Query: 525 ELLGLYDQIIYMEDGQIAETGSLEELLKQGGRFRSFYTLE 564 Sbjct: 549 -- 586 >STM0984 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 582 Score = 197 bits (501), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 148/562 (26%), Positives = 283/562 (50%), Gaps = 16/562 (2%) Query: 13 KVLLTVTLLCSILTSAAGVLIALILQKVIDAAL-QGDRQLFREIXXXXXXXXXXXGVFGF 71 K L V + IL +A+ + +L+ ++D + DR + + G+ + Sbjct: 24 KAGLIVAGIALILNAASDTFMLSLLKPLLDDGFGKTDRSVLLWMPLVVIGLMILRGITSY 83 Query: 72 IYSLCSKALIRNVTISLRERVFRGVFGQKPETFSATNTADYLSALTNDIKLLEDNYIQPM 131 I S C + V +++R R+F + G F +T LS +T D + + + + Sbjct: 84 ISSYCISWVSGKVVMTMRRRLFGHMMGMPVAFFDKQSTGTLLSRITYDSEQVASSSSGAL 143 Query: 132 LQVLQNVVVFAVSLGILLYLSPLVTVILIGCMVLMFAIPSLFGKALQHKQSEVSSQMSAF 191 + V++ ++ Y S +++IL+ ++ + K + + + M Sbjct: 144 ITVVREGASIIGLFIMMFYYSWQLSIILVVLAPIVSIAIRVVSKRFRSISKNMQNTMGQV 203 Query: 192 TASLKDLLSGYEVITSYAMGKYAGDTFKQQNH--RAAGVRFAADRLFAFNESVSQTLAIL 249 T S + +L G++ + + + F + ++ R G++ + + ++ + Q +A L Sbjct: 204 TTSAEQMLKGHKEVLIFGGQEVETKRFDKVSNKMRLQGMKMVSAS--SISDPIIQLIASL 261 Query: 250 TQFAVVFIAAYLIITGRLSAGSLVALVQLSGGFVGPMLAIMENLPKIQ-GIKPVRERIDW 308 V++ A++ + L+AG++ + + P+ ++ + Q G+ + Sbjct: 262 ALAFVLYAASFPSVMDSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFAI 321 Query: 309 LTGEKQEPDAEGLPPVFNSTLEARHLQFSYKPGN--PVIQDISLSLEKGKKYALVGPSGC 366 L E+++ + + + LE R++ F+Y PG P +++I+L + GK ALVG SG Sbjct: 322 LDSEQEKDEGKRVIDRATGDLEFRNVTFTY-PGREVPALRNINLKIPAGKTVALVGRSGS 380 Query: 367 GKSTLVKLLTGYYDRFDGSIELDGTDVRRLDREKLQQMVSTIHQNVYMFDSDIRHNICLH 426 GKST+ L+T +YD +G I +DG D+R L+ V+ + QNV++F+ + +NI Sbjct: 381 GKSTIASLITRFYDIDEGHILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYA 440 Query: 427 --EEFSEQMLDTALHTSGIHKFLQNTPDGLLSPVGENGLQLSGGQRQRIAVARALIRNKP 484 EE+S + ++ A + F+ +GL + +GENG+ LSGGQRQRIA+ARAL+R+ P Sbjct: 441 RTEEYSREQIEEAARMAYAMDFINKMDNGLDTIIGENGVLLSGGQRQRIAIARALLRDSP 500 Query: 485 ILVLDEGTSAIDMQTAYEIESRL--LKINELTLITITHNMNEELLGLYDQIIYMEDGQIA 542 IL+LDE TSA+D ++ I++ L L+ N +L+ I H ++ + D+I+ +EDG I Sbjct: 501 ILILDEATSALDTESERAIQAALDELQKNRTSLV-IAHRLST--IEQADEIVVVEDGIIV 557 Query: 543 ETGSLEELLKQGGRFRSFYTLE 564 E G+ ELL Q G + + ++ Sbjct: 558 ERGTHSELLAQHGVYAQLHKMQ 579 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,634,772 Number of extensions: 1529002 Number of successful extensions: 20411 Number of sequences better than 1.0e-05: 1940 Number of HSP's gapped: 18032 Number of HSP's successfully gapped: 2116 Length of query: 572 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 460 Effective length of database: 25,461,023 Effective search space: 11712070580 Effective search space used: 11712070580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)