BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0530.0021.p (323 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM3601 [M] COG2222 Predicted phosphosugar isomerases 264 1e-70 STM0572 [M] COG2222 Predicted phosphosugar isomerases 241 1e-63 SMc03139 [M] COG2222 Predicted phosphosugar isomerases 117 2e-26 STM4540 [M] COG2222 Predicted phosphosugar isomerases 109 7e-24 yhfN [M] COG2222 Predicted phosphosugar isomerases 86 1e-16 >STM3601 [M] COG2222 Predicted phosphosugar isomerases Length = 325 Score = 264 bits (675), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 133/324 (41%), Positives = 189/324 (58%), Gaps = 1/324 (0%) Query: 1 MEIKDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFV 60 M +K+ +S+I + E GG+KH+Y+VACGGS AA + ++KEA+ +YNS EF+ Sbjct: 2 MGMKETVSNIVTSQAEKGGVKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFI 61 Query: 61 HATPAMLDEQCIVVLCSLNA-TAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVY 119 + P L E +VV+ S T ET++A ++A + GA I +T + + + Y Y Sbjct: 62 NNPPVALGENAVVVVASHKGNTPETIKAAEIARQHGAPVIGLTWIMDSPLVAHCDYVETY 121 Query: 120 SNGEQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASR 179 + G+ + + L + E+L Q E YAHY + +K++ IV A + A Sbjct: 122 TFGDGKDIAGEKTMKGLLSAVELLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQA 181 Query: 180 FADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIP 239 FA EYKDD++ Y +ASG Y AY + C FMEMQW H+ +HSGE+FHGPFE TD N P Sbjct: 182 FAQEYKDDKVIYTVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTP 241 Query: 240 IILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERD 299 SEG TR +DERAL FL +Y +R+ V+DAKELG++ I +V +YF + V Sbjct: 242 FFFQFSEGNTRAVDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPV 301 Query: 300 IVSRMAEIRNHSMNMRRYMWKVEY 323 +AE R H + RRYMWKVEY Sbjct: 302 YNRALAEARQHPLTTRRYMWKVEY 325 >STM0572 [M] COG2222 Predicted phosphosugar isomerases Length = 328 Score = 241 bits (616), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 128/324 (39%), Positives = 181/324 (55%), Gaps = 8/324 (2%) Query: 4 KDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFVHAT 63 + IISDI K ++ ++FV CGGS + G Y + EA S+EFVHAT Sbjct: 9 RRIISDILGKQN----IERVWFVGCGGSLTGFWPGKYFLDCEASKLAVGYITSNEFVHAT 64 Query: 64 PAMLDEQCIVVLCSLNA-TAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVYSNG 122 P L + +V+L S TAETV A ++A E+GA TI + P T + Y + Y Sbjct: 65 PKALGKNSVVILASQQGNTAETVAAARVAREKGAATIGLVYQPDTPLCEYSDYIIEYQWA 124 Query: 123 ---EQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASR 179 E +Q + L ++ EIL Q E YA Y + AF + +V A+ ++ A R Sbjct: 125 RYPETVDPAQQKAAYSLWLALEILAQTEGYAQYDELVSAFGRFSDVVHGAQRQVQEDAQR 184 Query: 180 FADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIP 239 FA E+KD+++ Y+M SGP++ A+ + C +EMQW ++ +HSGEYFHGPFE T+ P Sbjct: 185 FAAEWKDEKVVYMMGSGPSFGAAHQESICILLEMQWINSASIHSGEYFHGPFEITEPGTP 244 Query: 240 IILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERD 299 IL S G TRPLD+RA+ F+ RY ++ +IDA +LGI + V EYF ++ V Sbjct: 245 FILLQSSGRTRPLDDRAIRFIERYQGKLQLIDADKLGIQDLSTDVGEYFCGLLHNCVLDV 304 Query: 300 IVSRMAEIRNHSMNMRRYMWKVEY 323 +A RNH + RRYMWKVEY Sbjct: 305 YNLALATARNHPLTTRRYMWKVEY 328 >SMc03139 [M] COG2222 Predicted phosphosugar isomerases Length = 337 Score = 117 bits (294), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 81/307 (26%), Positives = 138/307 (44%), Gaps = 5/307 (1%) Query: 19 GLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFVHATPAMLDEQCIVVLCSL 78 G ++++F+ GG+ + L+Q+ +R F I +E L E+ IVV+ SL Sbjct: 34 GAENVFFLGTGGAAILMQPAAQLLQRRSR-FPAFIDLPAELFITGSVNLTEKSIVVIPSL 92 Query: 79 NATA-ETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVV-YSNGEQQIYSQGNQSSVL 136 + T E+V + A E GA +++ G T + G + V ++ + S QS L Sbjct: 93 SGTTKESVALLAKAKEAGATVLSLVGHEETPLGKGGDHAFVNFAEDDTSCESFYLQS--L 150 Query: 137 RISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASRFADEYKDDEIFYVMASG 196 I+ I+ +Y ++ ++ + + A FA + V +G Sbjct: 151 FIALSIMRHRGEIENYDQIAGELERLPGLLLEVKRGYEDKAETFARVLAGSDYHIVTGAG 210 Query: 197 PAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIPIILFVSEGATRPLDERA 256 A+ A+ C EMQW P+H+ ++FHG E +K + +ILF E A RPL +R Sbjct: 211 NAWPEAFYYGMCILEEMQWIRTRPVHASDFFHGTLELVEKGVSVILFKGEDALRPLADRV 270 Query: 257 LTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERDIVSRMAEIRNHSMNMRR 316 F Y R+TV+D + + I V +++ V I + + +RNH + RR Sbjct: 271 QNFAPNYTDRLTVLDTADFALPGISQEVRGLVSPIVLATVLERISAHLEVMRNHPLTTRR 330 Query: 317 YMWKVEY 323 Y +V Y Sbjct: 331 YYKRVAY 337 >STM4540 [M] COG2222 Predicted phosphosugar isomerases Length = 343 Score = 109 bits (272), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 78/315 (24%), Positives = 138/315 (43%), Gaps = 20/315 (6%) Query: 18 GGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFVHATPAMLDEQCIVVLCS 77 G ++F + GGS A + + + KE T + ++E +H L++ +V+ S Sbjct: 40 AGFSSLFFASVGGSLAPMMAINEF-AKELTTLPVYVEQAAELIHKGNKRLNKDSVVITLS 98 Query: 78 LNA-TAETVQAVQLANERGAYTIAMTGFPTTEMATNGMY--PVVYSNGEQQIYSQGNQSS 134 + T E+V + +G +A+T + +A + P+ + NG + Y Sbjct: 99 KSGDTKESVAIAEWCKAQGIRVVAITKNADSPLAQAATWHIPMRHKNGVEYEYML----- 153 Query: 135 VLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASRFADEYKDDEIFYVMA 194 + + F +L + +A Y + + + A+ A A Y + + + Sbjct: 154 LYWLFFRVLSRNNEFASYDRFASQLEILPANLLKAKQKFDPQADAIASRYHNSDYMMWVG 213 Query: 195 SGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIPIILFVSEGATRPLDE 254 + Y + C EMQW+ P+ S E+FHG E +K++P+IL EG R LDE Sbjct: 214 GAEMWGEVYLFSMCILEEMQWKRTRPVSSAEFFHGALELLEKDVPLILVKGEGKCRALDE 273 Query: 255 RALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIP--VERDIVSRMA----EIR 308 R F ++ + VID K ++ IDD IM P V +V R+A + Sbjct: 274 RVERFASKITDNLVVIDPKAYALDGIDDE-----FRWIMAPCVVSTLLVDRLAAHFEKYT 328 Query: 309 NHSMNMRRYMWKVEY 323 HS+++RRY + +Y Sbjct: 329 GHSLDIRRYYRQFDY 343 >yhfN [M] COG2222 Predicted phosphosugar isomerases Length = 347 Score = 85.5 bits (210), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 73/306 (23%), Positives = 134/306 (43%), Gaps = 24/306 (7%) Query: 20 LKHIYFVACGGSQAAIFSGHYLIQK--EARTFGTSIYNSSEFVHATPAMLDEQCIVV-LC 76 + IYFVACG A + +L + + + + S + EF TP LD++C V+ + Sbjct: 51 IDRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGW---EFCDNTPYRLDDRCAVIGVS 107 Query: 77 SLNATAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVY-SNGEQQIYSQGNQSSV 135 T E ++A++L GA T A T + + + + + Y ++ +I+ S V Sbjct: 108 DYGKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV 167 Query: 136 LRISFEILHQFEAYAHYQAALEAFTKVDSIVAS-ARTYIK--RIASRFADEYKDDEIFYV 192 L E++ + A ++ + + RT+ + R A ++ + Y Sbjct: 168 L----EMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWP---MIYT 220 Query: 193 MASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIPIILFVSEGATRPL 252 +A+GP Y ME W H + SGE+ HGP E + +P + + +R Sbjct: 221 VAAGPLRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHT 280 Query: 253 DERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIM-IPVERDIVSRMAEIRNHS 311 ERA+ F+ + V VID E I + + +M +P+E + ++ ++H+ Sbjct: 281 TERAINFVKQRTDNVIVIDYAE-----ISQGLHPWLAPFLMFVPMEW-LCYYLSIYKDHN 334 Query: 312 MNMRRY 317 + RRY Sbjct: 335 PDERRY 340 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.133 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,268,004 Number of extensions: 826125 Number of successful extensions: 11415 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 11403 Number of HSP's successfully gapped: 15 Length of query: 323 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 216 Effective length of database: 26,037,693 Effective search space: 5624141688 Effective search space used: 5624141688 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)