BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0529.0028.p (277 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2476 [R] COG1161 Predicted GTPases 319 3e-87 BS_ylqF [R] COG1161 Predicted GTPases 314 1e-85 lin1311 [R] COG1161 Predicted GTPases 275 4e-74 SA1086 [R] COG1161 Predicted GTPases 268 9e-72 L128951 [R] COG1161 Predicted GTPases 256 4e-68 >BH2476 [R] COG1161 Predicted GTPases Length = 284 Score = 319 bits (818), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 154/277 (55%), Positives = 204/277 (73%), Gaps = 6/277 (2%) Query: 1 MTKARRQIEDKLKLIDVCIELLDSRLPLSSRNPMIDDILRDKPRLIILNKADLADPEATR 60 M KARR++ +KLKLIDV IELLD+R+PLSSRNPM+D+I+ KPRL++LNK DLADP T+ Sbjct: 10 MAKARREVTEKLKLIDVVIELLDARVPLSSRNPMMDEIVAHKPRLVLLNKDDLADPSKTK 69 Query: 61 KWLAFFKAEGHVAHPVDAATGTGIKEIPEQVKLLLKDKIDRQIAKGMNPRPSRALIVGIP 120 +W FF+ G P++A TG G+ I + L + I++Q AKGM PR RA+I+GIP Sbjct: 70 EWTRFFEEGGATVLPINAQTGQGVSRISPACQTLAQALIEKQRAKGMKPRAIRAMILGIP 129 Query: 121 NVGKSTLINRMAGKNIAATGDRPGVTKGQQWIKTGGNLELLDTPGILWPKFEDQEVGYRL 180 NVGKSTLINR+A K IA GDRPG+TK QQWIK G LELLDTPGILWPKF+DQ G+RL Sbjct: 130 NVGKSTLINRLASKRIAKVGDRPGITKQQQWIKVGKELELLDTPGILWPKFDDQATGFRL 189 Query: 181 AVTGAIKEEILNIEDIAFFAVKYLVKDYGASFQERFGIGQLPEDLDNPDEIVAVMEAVGR 240 A TGAIK+E+L+ +D+A F ++Y+ + Y +R+ + +LPED V + +A+G+ Sbjct: 190 AATGAIKDELLDFQDVALFVLRYMREMYPDRLMDRYKLNELPED------GVTLFDAIGK 243 Query: 241 KRGCLISGGRVDLEKASRILLHDLRAGKLGRFTLETP 277 KRG L+SGG +D +K + ++L +LRAG LGR TLE P Sbjct: 244 KRGHLLSGGYIDYDKTAEMILRELRAGTLGRITLEVP 280 >BS_ylqF [R] COG1161 Predicted GTPases Length = 282 Score = 314 bits (804), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 146/277 (52%), Positives = 206/277 (74%), Gaps = 6/277 (2%) Query: 1 MTKARRQIEDKLKLIDVCIELLDSRLPLSSRNPMIDDILRDKPRLIILNKADLADPEATR 60 M KARR++ +KLKLID+ EL+D+R+P+SSRNPMI+DIL++KPR+++LNKAD AD T+ Sbjct: 10 MAKARREVTEKLKLIDIVYELVDARIPMSSRNPMIEDILKNKPRIMLLNKADKADAAVTQ 69 Query: 61 KWLAFFKAEGHVAHPVDAATGTGIKEIPEQVKLLLKDKIDRQIAKGMNPRPSRALIVGIP 120 +W F+ +G + +++ G G+ +I K +L++K DR AKG+ PR RALI+GIP Sbjct: 70 QWKEHFENQGIRSLSINSVNGQGLNQIVPASKEILQEKFDRMRAKGVKPRAIRALIIGIP 129 Query: 121 NVGKSTLINRMAGKNIAATGDRPGVTKGQQWIKTGGNLELLDTPGILWPKFEDQEVGYRL 180 NVGKSTLINR+A KNIA TGDRPG+T QQW+K G LELLDTPGILWPKFED+ VG RL Sbjct: 130 NVGKSTLINRLAKKNIAKTGDRPGITTSQQWVKVGKELELLDTPGILWPKFEDELVGLRL 189 Query: 181 AVTGAIKEEILNIEDIAFFAVKYLVKDYGASFQERFGIGQLPEDLDNPDEIVAVMEAVGR 240 AVTGAIK+ I+N++D+A F +++L + Y +ER+G+ ++PED I + +A+G Sbjct: 190 AVTGAIKDSIINLQDVAVFGLRFLEEHYPERLKERYGLDEIPED------IAELFDAIGE 243 Query: 241 KRGCLISGGRVDLEKASRILLHDLRAGKLGRFTLETP 277 KRGCL+SGG ++ +K + +++ D+R K GR + E P Sbjct: 244 KRGCLMSGGLINYDKTTEVIIRDIRTEKFGRLSFEQP 280 >lin1311 [R] COG1161 Predicted GTPases Length = 287 Score = 275 bits (704), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 138/277 (49%), Positives = 195/277 (70%), Gaps = 6/277 (2%) Query: 1 MTKARRQIEDKLKLIDVCIELLDSRLPLSSRNPMIDDILRDKPRLIILNKADLADPEATR 60 M KARR++ +KLKL+DV EL+D+R+PLSS NPM+++I+ K R+IILNKAD AD + T+ Sbjct: 10 MAKARREVTEKLKLVDVIFELVDARIPLSSSNPMLEEIIHQKRRVIILNKADTADEKTTK 69 Query: 61 KWLAFFKAEGHVAHPVDAATGTGIKEIPEQVKLLLKDKIDRQIAKGMNPRPSRALIVGIP 120 +W+ +F +G A V+A G G+ +I + + L+ +K +R +KGM PR RA+I+GIP Sbjct: 70 EWIDYFAEKGLPAVAVNAQEGKGLFKIEQAAEKLMAEKFERLRSKGMKPRAIRAMILGIP 129 Query: 121 NVGKSTLINRMAGKNIAATGDRPGVTKGQQWIKTGGNLELLDTPGILWPKFEDQEVGYRL 180 NVGKSTLINR+A KNIA TG++PGVTK QQWIK G LELLDTPGILWPKFEDQE+GY+L Sbjct: 130 NVGKSTLINRLAKKNIARTGNKPGVTKAQQWIKVGKTLELLDTPGILWPKFEDQEIGYKL 189 Query: 181 AVTGAIKEEILNIEDIAFFAVKYLVKDYGASFQERFGIGQLPEDLDNPDEIVAVMEAVGR 240 A+TGAIK+++L +E+IA + +++L K Y Q + ++ ED P E +A + Sbjct: 190 ALTGAIKDDLLQMEEIAGYGLRFLEKHYPDRLQNWLKVEKVSED---PIETLAF---IAE 243 Query: 241 KRGCLISGGRVDLEKASRILLHDLRAGKLGRFTLETP 277 KRG L D +A+ ++ ++R KLGR + + P Sbjct: 244 KRGLLDRYNDPDYSRAAETVVREIRQQKLGRMSFDFP 280 >SA1086 [R] COG1161 Predicted GTPases Length = 294 Score = 268 bits (684), Expect = 9e-72, Method: Compositional matrix adjust. Identities = 124/275 (45%), Positives = 193/275 (70%), Gaps = 5/275 (1%) Query: 1 MTKARRQIEDKLKLIDVCIELLDSRLPLSSRNPMIDDILRDKPRLIILNKADLADPEATR 60 M KA+R++ ++LK +DV EL+D+R+P SSRNPMID+++ KPR++ILNK D+++ Sbjct: 10 MAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMIDEVINQKPRVVILNKKDMSNLNEMS 69 Query: 61 KWLAFFKAEGHVAHPVDAATGTGIKEIPEQVKLLLKDKIDRQIAKGMNPRPSRALIVGIP 120 KW FF +G+ VDA G +K++ +K +R+ AKG+ PR RA+IVGIP Sbjct: 70 KWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIKATAEKFEREKAKGLKPRAIRAMIVGIP 129 Query: 121 NVGKSTLINRMAGKNIAATGDRPGVTKGQQWIKTGGNLELLDTPGILWPKFEDQEVGYRL 180 NVGKSTLIN++A ++IA TG++PGVTK QQWIK L+LLDTPGILWPKFED+EVG +L Sbjct: 130 NVGKSTLINKLAKRSIAQTGNKPGVTKQQQWIKVSNALQLLDTPGILWPKFEDEEVGKKL 189 Query: 181 AVTGAIKEEILNIEDIAFFAVKYLVKDYGASFQERFGIGQLPEDLDNPDEIVAVMEAVGR 240 ++TGAIK+ I++++++A + + +L+++ A + + I ++PED EI+A +A+G+ Sbjct: 190 SLTGAIKDSIVHLDEVAIYGLNFLIQNDLARLKSHYNI-EVPEDA----EIIAWFDAIGK 244 Query: 241 KRGCLISGGRVDLEKASRILLHDLRAGKLGRFTLE 275 KRG + G +D E ++++D+R K+G + + Sbjct: 245 KRGLIRRGNEIDYEAVIELIIYDIRNAKIGNYCFD 279 >L128951 [R] COG1161 Predicted GTPases Length = 282 Score = 256 bits (653), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 128/275 (46%), Positives = 182/275 (66%), Gaps = 9/275 (3%) Query: 1 MTKARRQIEDKLKLIDVCIELLDSRLPLSSRNPMIDDILRDKPRLIILNKADLADPEATR 60 M+KARRQ+++ LK +D IEL+D+RLP+SSRNPM+ I+ DKPR+I LNKADLAD A Sbjct: 10 MSKARRQVQENLKYVDFVIELVDARLPISSRNPMLGQIIGDKPRIIALNKADLADSNAVA 69 Query: 61 KWLAFFKAEGHVAHPVDAATGTGIKEIPEQVKLLLKDKIDRQIAKGMNPRPSRALIVGIP 120 +W+ FF +G V +++ K + K L+ DK+ +G+ R +I+GIP Sbjct: 70 QWIEFFDEQGFVTLAINSKEEYTAKRLTVAAKKLMADKVAHDKERGIAHTTLRTMIIGIP 129 Query: 121 NVGKSTLINRMAGKNIAATGDRPGVTKGQQWIKTGGNLELLDTPGILWPKFEDQEVGYRL 180 N GKSTL+NR+AGK +A TG+RPGVTKGQQWI+T LELLDTPGILWPKFEDQ VG +L Sbjct: 130 NAGKSTLMNRLAGKKVAVTGNRPGVTKGQQWIRTNKELELLDTPGILWPKFEDQLVGLKL 189 Query: 181 AVTGAIKEEILNIEDIAFFAVKYLVKDYGASFQERFGIGQLPEDLDNPDEIVAVMEAVGR 240 A+TGAIK+++L ++++ F + Y +K+Y +R+ + + +L+ PD +ME R Sbjct: 190 ALTGAIKDDLLPMDEVTIFGLNYFLKNYRTVTMKRYRMTEEDLELEIPD---LIMELTAR 246 Query: 241 KRGCLISGGRVDLEKASRILLHDLRAGKLGRFTLE 275 G R D ++ + + D+R GKLG F L+ Sbjct: 247 ------FGFRDDYDRFYNMFVKDVRDGKLGLFCLD 275 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.140 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,044,729 Number of extensions: 984528 Number of successful extensions: 14587 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 14563 Number of HSP's successfully gapped: 23 Length of query: 277 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 172 Effective length of database: 26,268,361 Effective search space: 4518158092 Effective search space used: 4518158092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)