BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0529.0027.p (206 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1030 [U] COG0681 Signal peptidase I 121 9e-28 lin1310 [U] COG0681 Signal peptidase I 109 4e-24 alr2975 [U] COG0681 Signal peptidase I 102 3e-22 SA0826 [U] COG0681 Signal peptidase I 102 6e-22 BS_sipT [U] COG0681 Signal peptidase I 100 1e-21 >BH1030 [U] COG0681 Signal peptidase I Length = 182 Score = 121 bits (303), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 64/157 (40%), Positives = 99/157 (63%), Gaps = 9/157 (5%) Query: 49 FKPFIVDGPSMQPNFHTGERVIVNEILYDIRSPQRGEVIVFHVPSEGRDFIKRVIGVAGD 108 F + V G SM+P + GE IVN++ Y+ P+R ++IVFH +E D+IKR+IG+ GD Sbjct: 35 FVSYEVRGESMEPTAYEGEMFIVNKLSYEFSEPKRFDLIVFHA-TETDDYIKRIIGLPGD 93 Query: 109 TVKVEGDVVTVNGQPVNESYIQGAIDEAHKNNALYNNKNFPNEDFTDGTVPEGHVFVMGD 168 T+++E D++ +N +P E Y+ DE + ++F E+ +P+G+VFV+GD Sbjct: 94 TIRMEDDILYINDEPYEEPYL----DEWKEGRPGKYTQDFVVEE----PIPDGYVFVLGD 145 Query: 169 NRSDSTDSRMIGYVPLGDIVGRADLIFWPVKDISVIN 205 NR S+DSR G VPL +IVG+ + FWPV + V+N Sbjct: 146 NRPRSSDSRAFGPVPLEEIVGKVGVRFWPVTKVGVLN 182 >lin1310 [U] COG0681 Signal peptidase I Length = 180 Score = 109 bits (272), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 60/158 (37%), Positives = 96/158 (60%), Gaps = 9/158 (5%) Query: 49 FKPFIVDGPSMQPNFHTGERVIVNEILYDIRSPQRGEVIVFHVPSEGRDFIKRVIGVAGD 108 F P +VDG SM P H+ +RVI+N R +VIVF ++G+++IKRVIG+ GD Sbjct: 31 FVPILVDGISMMPTLHSDDRVIINRF----GEVDRFDVIVFR-EADGKEYIKRVIGLPGD 85 Query: 109 TVKVEGDVVTVNGQPVNESYIQGAIDEAHKNNALYNNKNFPNEDFTDG-TVPEGHVFVMG 167 TV+ + D + +NG+ +E Y+ E K+ Y ++ ++D DG +P+ FV+G Sbjct: 86 TVEYKADQLYINGKKYDEPYLD-TYKEKLKDG--YLTDDYSSKDQLDGGKIPKDTYFVLG 142 Query: 168 DNRSDSTDSRMIGYVPLGDIVGRADLIFWPVKDISVIN 205 DNR S DSR+IG +PL ++G + +WP++D +I+ Sbjct: 143 DNRRASKDSRIIGPIPLSKVLGTTPICYWPIEDAKLID 180 >alr2975 [U] COG0681 Signal peptidase I Length = 359 Score = 102 bits (255), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 7/163 (4%), Positives = 19/163 (11%), Gaps = 24/163 (14%) Query: 50 KPFIVDGPSMQPNFHTGERVIVNEILYDIRSPQRGEVIVFHVPSE--------GRDFIKR 101 + + E + + E + Sbjct: 44 ETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEE 103 Query: 102 VIGVAGDTVKVEGDVVTVNGQPVNESYIQGAIDEAHKNNALYNNKNFPNEDFTDGTVPEG 161 + + E V + + + + Sbjct: 104 QLAALEEASLDEPVTVWMKLDTGMHRLGVRP----------------EQAEAFYHRLTQC 147 Query: 162 HVFVMGDNRSDSTDSRMIGYVPLGDIVGRADLIFWPVKDISVI 204 N + F K Sbjct: 148 KNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRS 190 >SA0826 [U] COG0681 Signal peptidase I Length = 191 Score = 102 bits (253), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 55/154 (35%), Positives = 81/154 (52%), Gaps = 3/154 (1%) Query: 51 PFIVDGPSMQPNFHTGERVIVNEILYDIRSPQRGEVIVFHVPSEGRDFIKRVIGVAGDTV 110 P+ + G SM P GERV VN I Y ++G V+VFH ++ D++KRVIGV GD V Sbjct: 29 PYTIKGESMDPTLKDGERVAVNIIGYKTGGLEKGNVVVFHA-NKNDDYVKRVIGVPGDKV 87 Query: 111 KVEGDVVTVNGQPVNESYIQGAIDEAHKN--NALYNNKNFPNEDFTDGTVPEGHVFVMGD 168 + + D + VNG+ +E Y+ + + + K+ PN + +P+G V+GD Sbjct: 88 EYKNDTLYVNGKKQDEPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGD 147 Query: 169 NRSDSTDSRMIGYVPLGDIVGRADLIFWPVKDIS 202 NR S DSR G + IVG+ FWP + Sbjct: 148 NREVSKDSRAFGLIDEDQIVGKVSFRFWPFSEFK 181 >BS_sipT [U] COG0681 Signal peptidase I Length = 193 Score = 100 bits (250), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 59/185 (31%), Positives = 94/185 (50%), Gaps = 7/185 (3%) Query: 18 KNPRKPKNEVLEXXXXXXXXXXXXXXXXXXXFKPFIVDGPSMQPNFHTGERVIVNEILYD 77 K +K N LE F+P++V+G SM P H GER+ VN+ + Sbjct: 11 KTAKKKTNTYLEWGKAIVIAVLLALLIRHFLFEPYLVEGSSMYPTLHDGERLFVNKTVNY 70 Query: 78 IRSPQRGEVIVFHVPSEGRDFIKRVIGVAGDTVKVEGDVVTVNGQPVNESYIQGAIDEAH 137 I +RG++++ + + ++KR+IG G+TV+++ D + +NG+ V E Y+ EA Sbjct: 71 IGELKRGDIVIINGETSKIHYVKRLIGKPGETVQMKDDTLYINGKKVAEPYLSKNKKEAE 130 Query: 138 KNNALYNNKNFPNEDFTDGTVPEGHVFVMGDNRSDSTDSRM-IGYVPLGDIVGRADLIFW 196 K DF VP+G FVMGDNR +S DSR +G + IVG + +F+ Sbjct: 131 KLGVSLTG------DFGPVKVPKGKYFVMGDNRLNSMDSRNGLGLIAEDRIVGTSKFVFF 184 Query: 197 PVKDI 201 P ++ Sbjct: 185 PFNEM 189 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.139 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,533,624 Number of extensions: 647927 Number of successful extensions: 1139 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 1085 Number of HSP's successfully gapped: 29 Length of query: 206 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 105 Effective length of database: 26,729,697 Effective search space: 2806618185 Effective search space used: 2806618185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 113 (48.1 bits)