BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0529.0017.p (70 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ML0393 [R] COG0637 Predicted phosphatase/phosphohexomutase 97 6e-21 STM1566 [C] COG0281 Malic enzyme 80 1e-15 >ML0393 [R] COG0637 Predicted phosphatase/phosphohexomutase Length = 346 Score = 97.1 bits (240), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 5/69 (7%), Positives = 10/69 (14%) Query: 2 VSDSRSPRDGRFIEEIGYYNPIEVPAVVKIDEEKALKWLQTGAQASDTVRNLLSKAGVMK 61 ++ + V + G + L K Sbjct: 22 TFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREK 81 Query: 62 KFHELKQQK 70 K Sbjct: 82 LIIASKVSG 90 >STM1566 [C] COG0281 Malic enzyme Length = 565 Score = 79.7 bits (195), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 4/70 (5%), Positives = 16/70 (22%), Gaps = 1/70 (1%) Query: 1 VVSDSRSPRDGRFIEEIGYYNPIEVPAVVKIDEEKALKWLQTGAQASDTVRNLLSKAGVM 60 ++ + F E + + ++ E + + Q + + Sbjct: 20 LLEFPLLNKGSAFSVEERRNFNLS-GLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLR 78 Query: 61 KKFHELKQQK 70 + Sbjct: 79 NIQDTNETLF 88 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.131 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 5,095,343 Number of extensions: 185030 Number of successful extensions: 294 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 2 Length of query: 70 Length of database: 38,378,431 Length adjustment: 42 Effective length of query: 28 Effective length of database: 33,534,403 Effective search space: 938963284 Effective search space used: 938963284 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 109 (46.6 bits)