BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0529.0012.p (279 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MT2351 [E] COG1168 Bifunctional PLP-dependent enzyme with beta-c... 151 1e-36 Rv2294 [E] COG1168 Bifunctional PLP-dependent enzyme with beta-c... 150 2e-36 Rv0075 [E] COG1168 Bifunctional PLP-dependent enzyme with beta-c... 137 1e-32 MT0081 [E] COG1168 Bifunctional PLP-dependent enzyme with beta-c... 137 1e-32 aq_1969 [E] COG0436 Aspartate/tyrosine/aromatic aminotransferase 98 1e-20 >MT2351 [E] COG1168 Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities Length = 387 Score = 151 bits (381), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 103/300 (34%), Positives = 147/300 (49%), Gaps = 37/300 (12%) Query: 1 VILQSPVYYPFYDVIRMNDRKVAINPLKLEDGHYVMDYEQLEELMSGGARLL-------- 52 VI+ SPVY PFY + + R+V PL+ G +D + L+E S Sbjct: 93 VIVNSPVYAPFYAFVSHDGRRVIPAPLR---GDGRIDLDALQEAFSSARASSGSSGNVAY 149 Query: 53 LLCSPHNPGGRVWERAELLRLGELCLRYGVTVISDEIHCDLALPGHKHIPFASLSKELSD 112 LLC+PHNP G V EL + E R+GV V+SDEIH L G + P+ LS ++ Sbjct: 150 LLCNPHNPTGSVHTADELRGIAERAQRFGVRVVSDEIHAPLIPSGARFTPY--LSVPGAE 207 Query: 113 ITLTTLAATKTFNLPGLQTSYIVTSN---PELRRKFEYKLKALSLHMSAFFAPEAVEAAY 169 ++A+K +NL GL+ + + +L R E S H+ A AA+ Sbjct: 208 NAFALMSASKAWNLGGLKAALAIAGREAAADLARMPEEVGHGPS-HLGVI----AHTAAF 262 Query: 170 NEGGEWLDQLIQHISSNAEYAISYLAEHLPQVKPMRPEATYLLWVNCRELGLD------- 222 GG WLD L++ + N + + EHLP V+ P+ TYL W++CRELG D Sbjct: 263 RTGGNWLDALLRGLDHNRTLLGALVDEHLPGVQYRWPQGTYLAWLDCRELGFDDAASDEM 322 Query: 223 PEGL---------KKLMYREAKVAFNEGSIFGTEGQGHLRINLACPRSTLTEALERFARA 273 EGL + A+VA + G +FG G GH+RIN A R+ L EA+ R +R+ Sbjct: 323 TEGLAVVSDLSGPARWFLDHARVALSSGHVFGIGGAGHVRINFATSRAILIEAVSRMSRS 382 >Rv2294 [E] COG1168 Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities Length = 673 Score = 150 bits (380), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 18/300 (6%), Positives = 42/300 (14%), Gaps = 37/300 (12%) Query: 1 VILQSPVYYPFYDVIRMNDRKVAINPLKLEDGHYVMDYEQLEELMSGGARLL-------- 52 + + I+ + + S Sbjct: 113 QLALLKELTEGLIEDDKVLLQQLISTIS---NWKNDLKTPSQAAASAIGERDRIFAHCYG 169 Query: 53 LLCSPHNPGGRVWERAELLRLGELCLRYGVTVISDEIHCDLALPGHKHIPFASLSKELSD 112 L + + +L L + L S+ Sbjct: 170 LYDAHLKACNVLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTN--TSQYELV 227 Query: 113 ITLTTLAATKTFNLPGLQTSYIVTSNPELRRKFEYKLKALS---LHMSAFFAPEAVEAAY 169 L A T Q+ Y + + + + Sbjct: 228 KLLVGSRARFTVVGDDDQSIYSWRG-----ARPQNLVLLSQDFPALKVIKLEQNYRSSGR 282 Query: 170 NEGGEWLDQLIQHISSNAEYAISYLAEHLPQVKPMRPEATYLLWVNCRELGLD------- 222 + +V E V + Sbjct: 283 ILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQY 342 Query: 223 PEGL---------KKLMYREAKVAFNEGSIFGTEGQGHLRINLACPRSTLTEALERFARA 273 + + + GT I + + + Sbjct: 343 KDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAF 402 >Rv0075 [E] COG1168 Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities Length = 673 Score = 137 bits (346), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 18/275 (6%), Positives = 54/275 (19%), Gaps = 5/275 (1%) Query: 1 VILQSPVYYPFYDVIRMNDRKVAINPLKLED-GHYVMDYEQLEELMSGGARLLLLCSPHN 59 + + I+ + + ++ Sbjct: 113 QLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYD 172 Query: 60 PGGRVWERAELLRLGELCLRYGVTVISDEIHCDLALPGHKHIPFASLSKELSDITLTTLA 119 + + L L + + + + + L Sbjct: 173 AHLKACNVLDFDDLILLPTLLLQANEEVRKRWQNKI-RYLLVDEYQDTNTSQYELVKLLV 231 Query: 120 ATKTFNLPGLQTSYIVTSNPELRRKFEYKLKALSLHMSAFFAPEAVEAAYNEGGEWLDQL 179 ++ + S R + L + E + + + L Sbjct: 232 GSRARFTVVGDDDQSIYSWRGARPQNL-VLLSQDFPALKVIKLEQNYRSSGRILKAANIL 290 Query: 180 IQHISSNAEYAISYLAEHLPQVKPMRPEATYLLWVNCRELGLDPEGLKKLMYREAKVAFN 239 I + E + + ++K + + + K Y+ Sbjct: 291 IANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQYK-DYAILY 349 Query: 240 EGSIFGTE-GQGHLRINLACPRSTLTEALERFARA 273 G+ + ++ + S T R Sbjct: 350 RGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIK 384 >MT0081 [E] COG1168 Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities Length = 673 Score = 137 bits (346), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 18/275 (6%), Positives = 54/275 (19%), Gaps = 5/275 (1%) Query: 1 VILQSPVYYPFYDVIRMNDRKVAINPLKLED-GHYVMDYEQLEELMSGGARLLLLCSPHN 59 + + I+ + + ++ Sbjct: 113 QLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYD 172 Query: 60 PGGRVWERAELLRLGELCLRYGVTVISDEIHCDLALPGHKHIPFASLSKELSDITLTTLA 119 + + L L + + + + + L Sbjct: 173 AHLKACNVLDFDDLILLPTLLLQANEEVRKRWQNKI-RYLLVDEYQDTNTSQYELVKLLV 231 Query: 120 ATKTFNLPGLQTSYIVTSNPELRRKFEYKLKALSLHMSAFFAPEAVEAAYNEGGEWLDQL 179 ++ + S R + L + E + + + L Sbjct: 232 GSRARFTVVGDDDQSIYSWRGARPQNL-VLLSQDFPALKVIKLEQNYRSSGRILKAANIL 290 Query: 180 IQHISSNAEYAISYLAEHLPQVKPMRPEATYLLWVNCRELGLDPEGLKKLMYREAKVAFN 239 I + E + + ++K + + + K Y+ Sbjct: 291 IANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQYK-DYAILY 349 Query: 240 EGSIFGTE-GQGHLRINLACPRSTLTEALERFARA 273 G+ + ++ + S T R Sbjct: 350 RGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIK 384 >aq_1969 [E] COG0436 Aspartate/tyrosine/aromatic aminotransferase Length = 340 Score = 98.2 bits (243), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 0/279 (0%), Positives = 0/279 (0%), Gaps = 13/279 (4%) Query: 1 VILQSPVYYPFYDVIRMNDRKVAINPLKLEDGHYVMDYEQLEELMSGGARLLLLCSPHNP 60 S + + ++ M M + Sbjct: 119 SKSGSADFLEALGAKVELLPEKVARLIEEVGF-GFMFAPLFHPAMKRVVSPRREVGVRSI 177 Query: 61 GGRVWERAELLRLGELCLRYGVTVISDEIHCDLALPGHKHIPFASLSKELSDITLTTLAA 120 + + L D+ L G K + ++++ Sbjct: 178 FNLIGPLTNPAGVKRYLLGVFSKEYVDKFAKALKNLGVKKAFVVHGEGGIDEVSVEGETY 237 Query: 121 TKTFNLPGLQTSYIVTSNPELRRKFEYKLKALSL-HMSAFFAPEAVEAAYN-EGGEWLDQ 178 + G+++ EL K + + + +V + + Sbjct: 238 VTEVSEEGIRSFTFSP--EELGVKRKSLSEVRVNSPEESVKVALSVLRGEEGTPRDMVLL 295 Query: 179 LIQHISSNAEYAISYLAEHLPQVKPMRPEATYLLWVN----CRELGLDPEGLKKLMYREA 234 +E A K L + Sbjct: 296 NASFGILVSERAGDIKE-AFEMAKESIDSGKALEVLEKYVE- 353 Query: 235 KVAFNEGSIFGTEGQGHLRINLACPRSTLTEALERFARA 273 Sbjct: 354 -- 390 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,308,790 Number of extensions: 864971 Number of successful extensions: 2136 Number of sequences better than 1.0e-05: 130 Number of HSP's gapped: 2088 Number of HSP's successfully gapped: 130 Length of query: 279 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 174 Effective length of database: 26,268,361 Effective search space: 4570694814 Effective search space used: 4570694814 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)