BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0526.0008.p (302 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_flgL [N] COG1344 Flagellin and related hook-associated proteins 184 1e-46 TM0082 [N] COG1344 Flagellin and related hook-associated proteins 118 1e-26 BH3620 [N] COG1344 Flagellin and related hook-associated proteins 105 9e-23 CAC2211 [N] COG1344 Flagellin and related hook-associated proteins 91 2e-18 TP0868 [N] COG1344 Flagellin and related hook-associated proteins 70 4e-12 >BS_flgL [N] COG1344 Flagellin and related hook-associated proteins Length = 298 Score = 184 bits (468), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 104/301 (34%), Positives = 177/301 (58%), Gaps = 4/301 (1%) Query: 3 IRVTQGMMSMQTXXXXXXXXXXXXDLADQASTGRKINKPSDNPVGVTYSLRYRAELASNE 62 +RVTQGM+ + L Q S+G+KI+K SD+PV SL+Y +L+ + Sbjct: 1 MRVTQGMIQQNSLRYIGSSYSKLDKLQSQISSGKKISKASDDPVVAMKSLKYNTQLSQVQ 60 Query: 63 QFQTNADAAVSWLDFTDSTMQQATDVMKRLKELTVQASSSTVPKSGLDAIKLEVEQLKDQ 122 Q+++NA A +WL+ T++ + + D++ +++EL V+A + T + AI +EV+QLK+Q Sbjct: 61 QYKSNASQAFTWLENTETNITEGIDILSKVRELAVEAQNDTNGEPERQAIGVEVKQLKEQ 120 Query: 123 LGNIGNAQIRGKYIFNGQNFDKPPYELSGTVTNFAQIDTDSGAVKYAIGDQSTFQVNTTG 182 L NI N Q+ G+YIFNG N DKPP +G T +D+ V I + + +VN+ Sbjct: 121 LLNIANTQVNGRYIFNGTNSDKPPVTDNGDGTYTI---SDTADVVVNISNNMSLKVNSDP 177 Query: 183 SEFFGASTDS-DNIYKVMDDLISALGSENYTGIAAQAGKIESRSTKMQAALSEVGARTNR 241 FG +++S N+++++D AL S + G+ + I+ S M A S++GAR NR Sbjct: 178 KSAFGGTSESGQNVFEMLDSFEKALNSGSLDGMDSVLNDIDHFSDGMSAERSDLGARYNR 237 Query: 242 VELVQGRLNERDLNLTTLQAKIEDADIAEVMIKSTTAQTIYEAALKSSASILQPSLMDFM 301 +ELV RL+ ++ T + + ED ++ EV+ + Q+++ A L +A I+QP+L+DF+ Sbjct: 238 LELVNTRLSAQEETATKVLSDNEDVELEEVITEFIAQQSVHRATLAVNAQIVQPTLIDFL 297 Query: 302 R 302 + Sbjct: 298 K 298 >TM0082 [N] COG1344 Flagellin and related hook-associated proteins Length = 304 Score = 118 bits (295), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 87/312 (27%), Positives = 159/312 (50%), Gaps = 20/312 (6%) Query: 3 IRVTQGMMSMQTXXXXXXXXXXXXDLADQASTGRKINKPSDNPVGVTYS---LRYRAELA 59 +RVT M+S + L DQ S+G K+ PSD+ V + L + EL Sbjct: 1 MRVTNKMISDRVLFNIQTSLKRIAKLHDQLSSGYKVRYPSDDAVVAKRASDILTRKREL- 59 Query: 60 SNEQFQTNADAAVSWLDFTDSTMQQATDVMKRLKELTVQASSSTVPKSGLDAIKLEVEQL 119 EQFQ + D ++++ DS +Q + + +RL+EL V+A++ T+ K+ +AI E++++ Sbjct: 60 --EQFQRSVDHVQTYVNAYDSALQTVSSITQRLRELLVRAANGTLSKAEREAIAEEIDKI 117 Query: 120 KDQLGNIGNAQIRGKYIFNGQNFDKPPYELSGTVTNFAQIDTDSGAVKYAIGDQSTFQVN 179 K+ L + N +I +YIF+G + P E + + + + + + S Sbjct: 118 KENLVEVANTRIGNEYIFSGYDVFSQPVENENGIWKISTLPASNKSRSILVLGHS-IDYG 176 Query: 180 TTGSEFFGASTDSDNIYKVMDDLISALGSE-NYTGIAAQAGKI--------ESRSTKMQA 230 T SE F + N++++++++ + L S N T I+ + + E R TK Sbjct: 177 VTASEVFELDS-GKNVFQMLEEVSATLRSNLNETQISERVSNVFLKELSSFEERITK--- 232 Query: 231 ALSEVGARTNRVELVQGRLNERDLNLTTLQAKIEDADIAEVMIKSTTAQTIYEAALKSSA 290 + +VG + +++V R+ + D T +K DADI E++ Q++ EAALKS++ Sbjct: 233 TIGKVGGVSRFIDMVNSRVQDLDFFFTEYLSKERDADITELVTDLAMQQSVLEAALKSAS 292 Query: 291 SILQPSLMDFMR 302 +LQ +L+DF+R Sbjct: 293 RVLQATLVDFVR 304 >BH3620 [N] COG1344 Flagellin and related hook-associated proteins Length = 395 Score = 105 bits (262), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 53/144 (36%), Positives = 81/144 (56%) Query: 3 IRVTQGMMSMQTXXXXXXXXXXXXDLADQASTGRKINKPSDNPVGVTYSLRYRAELASNE 62 +RVTQGM++ + +L DQ STG++I + S +PV +RYR ++A E Sbjct: 1 MRVTQGMLAGNSLRHISQGYMRLGELQDQLSTGKRITRASQDPVVAMKGIRYRTQVAEVE 60 Query: 63 QFQTNADAAVSWLDFTDSTMQQATDVMKRLKELTVQASSSTVPKSGLDAIKLEVEQLKDQ 122 QF+ N +W+D D+ + +ATD + R+ EL QA++ T ++ I E+ QL + Sbjct: 61 QFKRNLREVYNWMDTADAALGEATDALARIHELATQAANDTYEETQRANIAKEIRQLYEH 120 Query: 123 LGNIGNAQIRGKYIFNGQNFDKPP 146 L +I N + GKYIFNG N PP Sbjct: 121 LQSIANTKNSGKYIFNGTNTTNPP 144 Score = 63.5 bits (153), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 28/76 (36%), Positives = 52/76 (68%) Query: 227 KMQAALSEVGARTNRVELVQGRLNERDLNLTTLQAKIEDADIAEVMIKSTTAQTIYEAAL 286 ++ A +E+GAR NRVE+++ R+ ++++ + + ED D+ +V+I T ++++ AAL Sbjct: 320 QVTAERAELGARVNRVEMLESRILQQEITAKRIMSDNEDVDLEKVIIDLTIQESVHRAAL 379 Query: 287 KSSASILQPSLMDFMR 302 A I+QP+LMDF+R Sbjct: 380 AVGARIMQPTLMDFLR 395 >CAC2211 [N] COG1344 Flagellin and related hook-associated proteins Length = 425 Score = 90.9 bits (224), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 43/147 (29%), Positives = 81/147 (55%) Query: 3 IRVTQGMMSMQTXXXXXXXXXXXXDLADQASTGRKINKPSDNPVGVTYSLRYRAELASNE 62 +R+T M++ D+ Q ++G+KI KPSDNP T S++ +++ N Sbjct: 1 MRITNRMLTDTFLTDMNTNLQNMRDIQSQLTSGKKIRKPSDNPYVATKSMQLNTDISINS 60 Query: 63 QFQTNADAAVSWLDFTDSTMQQATDVMKRLKELTVQASSSTVPKSGLDAIKLEVEQLKDQ 122 Q+ TN + + WL+ TD+ + QA ++++R+KEL + A + + D+IK E+ Q + Sbjct: 61 QYNTNINNTIYWLNQTDTALNQAGNIVQRIKELLISAGNGGYTQDQRDSIKAELNQRISE 120 Query: 123 LGNIGNAQIRGKYIFNGQNFDKPPYEL 149 N+ N+ G+Y+F G ++ P ++ Sbjct: 121 FSNVINSSFSGQYLFGGTRGNEKPLDI 147 Score = 60.5 bits (145), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 37/104 (35%), Positives = 58/104 (55%), Gaps = 2/104 (1%) Query: 198 VMDDLISALGSENYTGIAAQAGKIESRSTKMQAALSEVGARTNRVELVQGRLNERDLNLT 257 ++ DL S G N A+A +++ + A S VGA NR++ Q E + N+T Sbjct: 323 IVTDLSSNSGVSNLNTKDAEA--VDALLENILAIRSTVGASQNRMDSAQKNNQENNYNMT 380 Query: 258 TLQAKIEDADIAEVMIKSTTAQTIYEAALKSSASILQPSLMDFM 301 + + ED DI E + QT+Y A+L++SA I+QP+LMD+M Sbjct: 381 LILSNTEDTDITESTMNYAALQTVYLASLQTSAKIIQPTLMDYM 424 >TP0868 [N] COG1344 Flagellin and related hook-associated proteins Length = 286 Score = 70.5 bits (171), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 68/280 (24%), Positives = 132/280 (47%), Gaps = 28/280 (10%) Query: 30 DQASTGRKINKPSDNPVGVTYSLRYRAELASNEQFQTNADAAVSWLDFTDSTMQQATDVM 89 ++ S+G +IN+ D+ G+ S + R+++ Q TNA V+++ T++ +Q+ TD+M Sbjct: 28 EKLSSGYRINRAGDDASGLAVSEKMRSQIRGLNQASTNASNGVNFIQVTEAYLQETTDIM 87 Query: 90 KRLKELTVQASSSTVPKSGLDAIKLEVEQLKDQLGNIG-NAQIRGKYIFNGQNFDKPPYE 148 +R++EL +QA++ I++EV QL ++ I +AQ FNG N Sbjct: 88 QRIRELAIQAANGIYSAEDRMQIQVEVSQLVAEVDRIASSAQ------FNGMNL------ 135 Query: 149 LSGTVTNFAQIDTDSGAVKYAIG---DQST-FQVNTTGSEFFGASTDSDNIYKVMDDLIS 204 L+G + + G++ + IG DQ + T + G D S Sbjct: 136 LTGRFSRTEGENVIGGSMWFHIGANMDQRMRVYIGTMTAVALGVRNGVDE---------S 186 Query: 205 ALGSENYTGIAAQAGKIESRSTKMQAALSEVGARTNRVELVQGRLNERDLNLTTLQAKIE 264 + E G I++ ++ +++G NR+E L+ NL +++I Sbjct: 187 IMSIETADSANKSIGTIDAALKRINKQRADLGGYQNRMEYTVVGLDIAAENLQAAESRIR 246 Query: 265 DADIAEVMIKSTTAQTIYEA--ALKSSASILQPSLMDFMR 302 DA+IA+ M++ T Q + ++ A+ + A+ S++ +R Sbjct: 247 DANIAKQMVEYTKNQVLTQSGTAMLAQANTSAQSILSILR 286 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.311 0.125 0.331 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,753,879 Number of extensions: 687190 Number of successful extensions: 1961 Number of sequences better than 1.0e-05: 60 Number of HSP's gapped: 1953 Number of HSP's successfully gapped: 63 Length of query: 302 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 196 Effective length of database: 26,153,027 Effective search space: 5125993292 Effective search space used: 5125993292 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 115 (48.9 bits)