BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0526.0001.p (476 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0696 [M] COG0463 Glycosyltransferases involved in cell wall b... 144 3e-34 TM0757_1 [M] COG0463 Glycosyltransferases involved in cell wall ... 103 5e-22 RSp0387 [M] COG0463 Glycosyltransferases involved in cell wall b... 99 2e-20 YPO0054 [M] COG0463 Glycosyltransferases involved in cell wall b... 80 9e-15 FN0542 [M] COG0463 Glycosyltransferases involved in cell wall bi... 79 1e-14 >lin0696 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 301 Score = 144 bits (363), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 12/226 (5%), Positives = 35/226 (15%), Gaps = 5/226 (2%) Query: 4 LSVCMIVKDEEALLSRCLESVKGIADEIIIVDTGSTDRTKQIAENFGAKIYDYVWSNDFA 63 S C + + + + I + + T+ + D Sbjct: 5 KSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTP 64 Query: 64 AARNESLRHAAGKWILVLDADEYLGSNDHSKWIEFLQKEKPQAQLAYTLPVINFTGEKDY 123 E R + + L + Sbjct: 65 GLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLN---KLREGKAPVI- 120 Query: 124 QEEMTTAPVTRLFPNFKGIHFERPIHEQLTRGGKEKLLHKKISLEIYHTGYQIQRVTEKS 183 + + + I + Sbjct: 121 -LAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHH 179 Query: 184 KHERNMSIFNEMKKANKLSAYDWFTLGNQYRFGKEDEEALQCYERA 229 E ++ ++ A+++ E + Sbjct: 180 FPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNE 225 >TM0757_1 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 200 Score = 103 bits (258), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 74/197 (37%), Positives = 108/197 (54%), Gaps = 8/197 (4%) Query: 4 LSVCMIVKDEEALLSRCLESVKGIADEIIIVDTGSTDRTKQIAENFGAKIYDYVWSNDFA 63 LSV MIVKDEE + R LES+K + DEI++VDTGSTDRT +I + K+Y + W NDF+ Sbjct: 6 LSVAMIVKDEEHNIRRALESIKDVVDEIVVVDTGSTDRTPEIVREYTDKLYFHEWKNDFS 65 Query: 64 AARNESLRHAAGKWILVLDADEYLGSNDHSKWIEFLQKEKPQAQLAYTLPVINFTGEKDY 123 ARN SL+ +W+L+LDADE FL+ Y LP +++ Sbjct: 66 EARNFSLKFPTCEWVLILDADEEASEEFRQNIRSFLESLPEDVNTVY-LPTVSYLDWDFK 124 Query: 124 QEEMTTAPVTRLFPNFKGIHFERPIHEQLTRGGKEKLLHKKISLEIYHTGYQIQRVTEKS 183 + E+ + P R+F N ++++ +H Q K K++H IYH GY R +K+ Sbjct: 125 RTEIASTP--RIFRN-GTVYYKNIVHNQAV--YKPKVVHA--PFVIYHYGYIWTRKLKKN 177 Query: 184 KHERNMSIFNEMKKANK 200 K+ER ++ E K K Sbjct: 178 KYERTATLIREQLKTVK 194 >RSp0387 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 362 Score = 99.0 bits (245), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 76/262 (29%), Positives = 121/262 (46%), Gaps = 23/262 (8%) Query: 4 LSVCMIVKDEEALLSRCLESVKGIADEIIIVDTGSTDRTKQIAENFGAKIYDYVWSNDFA 63 L++ +I ++E A + RCL S + D +I++DTGSTD T IAE GA+++ + W +DF+ Sbjct: 13 LALVVIARNEAACIERCLRSARPHVDRMIVLDTGSTDATVAIAEACGAQVHHFTWVDDFS 72 Query: 64 AARNESLRHAAGKWILVLDADEYLGSNDHSKWIEFLQKEKPQA-QLAYTLPVINFTGEKD 122 AARN +L+ A W LV+DADE W+E E +A L V+ D Sbjct: 73 AARNAALQFADADWNLVMDADE---------WLESGAGELREACTFGPLLGVVCIQSLDD 123 Query: 123 Y--QEEMTTAPVTRLFPNFKGIHFERPIHEQLTRGGKEKLLHKKISLEIYHTGYQIQRVT 180 + T + RL P +G+ ++ +HEQ L ++ L H GY ++ Sbjct: 124 AGGATQRTNTWIPRLLP--RGVRYQGRVHEQPV----ASLRRVRLPLVFGHDGYVSAKL- 176 Query: 181 EKSKHERNMSIFNEMKKANKLSAYDWFTLGNQYRFGKEDEEALQCYERAAKGATAKEAWY 240 + K RN ++ + Y + LG + +D A A Y Sbjct: 177 -EKKRGRNAALLHAELADRPDDPYVLYQLGKETEINGKDPAQAADLYAQAAAGAPANAPY 235 Query: 241 SH--CLMGLISLYFKNGRLDSS 260 H C+ L+ L ++GRLD + Sbjct: 236 RHDACIRLLLCLR-QSGRLDEA 256 >YPO0054 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 260 Score = 79.7 bits (195), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 55/154 (35%), Positives = 86/154 (55%), Gaps = 4/154 (2%) Query: 3 QLSVCMIVKDEEALLSRCLESVKGIADEIIIVDTGSTDRTKQIAENFGAKIYDYVWSNDF 62 +LSV MI K+E +LL+ CL SV ADEII++D+GS D T+ +A+ FGAK+Y + + Sbjct: 6 RLSVVMITKNEASLLTDCLASV-AWADEIIVLDSGSEDETRALAQQFGAKVYSNINWPGY 64 Query: 63 AAARNESLRHAAGKWILVLDADEYLGSNDHSKWIEFLQKEKPQAQLAYTLPVIN-FTGEK 121 R + ++A+G +IL+LDADE + + + IE + P+ Y+ N F G Sbjct: 65 GKQRQLAQQYASGDYILMLDADERV-TPELKIAIESVLL-APEEGAVYSCSRRNLFLGRF 122 Query: 122 DYQEEMTTAPVTRLFPNFKGIHFERPIHEQLTRG 155 VTRL+P+ + + + +HE L G Sbjct: 123 MRHSGWYPDRVTRLYPHHQYRYNDNLVHESLDSG 156 >FN0542 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 263 Score = 79.0 bits (193), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 60/175 (34%), Positives = 88/175 (50%), Gaps = 15/175 (8%) Query: 4 LSVCMIVKDEEALLSRCLESVKGIADEIIIVDTGSTDRTKQIAENFGAKIYDYVWSNDFA 63 L+V MI +EE L R L+SV+ ADEI+IVD+GSTD+T++IA+ FGAK W + Sbjct: 7 LTVAMITLNEEKNLERTLKSVQDFADEIVIVDSGSTDKTEEIAKKFGAKFVYQQWLG-YG 65 Query: 64 AARNESLRHAAGKWILVLDADEYLGSNDHSKWIEFLQKEKPQAQLAYTLPVINFT----G 119 RN ++ + WIL +DADE + +K ++ K ++ Y + INF Sbjct: 66 PQRNRAIELSTSDWILNIDADEEISPELANK----IKGIKENSR--YKVYKINFMSVCFN 119 Query: 120 EKDYQEEMTTAPVTRLFPNFKGIHFERPIHEQLTRGGKEKLLHKKISLEIYHTGY 174 +K + RLF G + E +HE+ + LHK IYH Y Sbjct: 120 KKIKHGGWSNTYRIRLFRKNAGSYNENSVHEEFVTNQEIVKLHK----YIYHHSY 170 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,212,910 Number of extensions: 1304866 Number of successful extensions: 2432 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 2477 Number of HSP's successfully gapped: 22 Length of query: 476 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 366 Effective length of database: 25,691,691 Effective search space: 9403158906 Effective search space used: 9403158906 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)