BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0051.p (314 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAE0876 [GER] COG0697 Permeases of the drug/metabolite transport... 90 4e-18 AF1223 [GER] COG0697 Permeases of the drug/metabolite transporte... 79 8e-15 TM1787 [GER] COG0697 Permeases of the drug/metabolite transporte... 75 1e-13 STM1787 [C] COG0374 Ni,Fe-hydrogenase I large subunit 75 1e-13 APE2537 [GER] COG0697 Permeases of the drug/metabolite transport... 71 2e-12 >PAE0876 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 292 Score = 90.1 bits (222), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 73/249 (29%), Positives = 127/249 (51%), Gaps = 8/249 (3%) Query: 27 GIVAISFSAIFIKWSAAPASVQGMYRLLFTSLLMLPFARPYSGAAFALRRKDWXXXXXXX 86 GI+AIS +AI I+ + A +RL F +L++L + G R + Sbjct: 8 GILAISSAAILIRLTPADPLAITFWRLAFATLIVLLIGV-WRGLELP-RGRALAYSIASG 65 Query: 87 XXXXXHFLLWMGSLKYTSVASSTMIMALEPVFIMLGAYILYKDRSTXXXXXXXXXXXFGV 146 HFL W+ SL T+VA+ST ++ + P+F ML +R + G Sbjct: 66 LFLAVHFLSWIPSLFLTTVAASTTLVNIHPIF-MLALSRRIGERVSGTTVAGVAVAVLGS 124 Query: 147 VLIGWGDIGLSSDNLKGDLLSVGGTVTVAGHLLIGQKLVARMPSYLYSLIVFITAAVVFA 206 VLI + S G++L++ G ++ AG+L +G+ + + ++ Y + + A++V Sbjct: 125 VLITF-----SPGGFAGNILAIIGALSFAGYLALGRIIRLSVGTFGYVAVAYGVASLVSL 179 Query: 207 IYNLLAGIPFLDYPPREWGIFVLLAVVPTVFGHILFNWLLQFTSATTVSMNILGEPVGAS 266 + +L Y + +F+L+A +P + GH +FN+LL A T++++ LGEPVGA+ Sbjct: 180 VTAVLLRANLTQYDAHTFLMFLLIASIPMMLGHTVFNYLLGKHRAITIAVSTLGEPVGAT 239 Query: 267 ILAFLLLGE 275 +LA +LGE Sbjct: 240 LLAVPILGE 248 >AF1223 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 275 Score = 79.3 bits (194), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 73/254 (28%), Positives = 115/254 (45%), Gaps = 18/254 (7%) Query: 27 GIVAISFSAIFIKWSAAPASVQGMYRLLFTSLLMLPFARPYSGAAFALRRKDWXXXXXXX 86 ++A+S +AIF ++ P V +RL + +L RP + R Sbjct: 10 AVLAVSSAAIFAFLASVPGVVAAFWRLTLSIPFLLLIYRPRK---IVMSRMS----VLAG 62 Query: 87 XXXXXHFLLWMGSLKYTSVASSTMIMALEPVFIMLGAYILYKDRSTXXXXXXXXXXXFGV 146 HF W+ SL + S+A ST I+ +F L AY L+ ++ GV Sbjct: 63 LALALHFSFWIESLFHASIAVSTTIVCTHSLFSGLFAY-LWGEKPRPNQIAGVLIAMVGV 121 Query: 147 VLIGWGDIGLSSDNLKGDLLSVGGTVTVAGHLLIGQKLVAR---MPSYLYSLIVFITAAV 203 L+ D + +L G LL++ G + AG AR PSYL ++ + +AAV Sbjct: 122 YLLSGAD---ARADLYGILLALAGAI--AGGYYFASARFARSVDFPSYL--IVTYSSAAV 174 Query: 204 VFAIYNLLAGIPFLDYPPREWGIFVLLAVVPTVFGHILFNWLLQFTSATTVSMNILGEPV 263 + LL P YP W L+A++P + GH L N+LL+ V+ +++GE V Sbjct: 175 FSLLACLLTSQPIFGYPASTWIYLALMALIPMLVGHSLLNYLLRQMEVLPVTASVIGEAV 234 Query: 264 GASILAFLLLGEQL 277 GA+ LA + L + L Sbjct: 235 GATALAAIFLNQTL 248 >TM1787 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 273 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 76/252 (30%), Positives = 124/252 (49%), Gaps = 11/252 (4%) Query: 28 IVAISFSAIFIKWSAAPASVQGMYRLLFTSLLMLPFARPYSGAAFALRRKDWXXXXXXXX 87 ++ SFS I IK S+ P + YR+ SL R F+L+++ Sbjct: 11 VLVASFSGILIKLSSLPPATIAFYRVFIASLFFFLVKRRVE--LFSLKKE--VLSLATGF 66 Query: 88 XXXXHFLLWMGSLKYTSVASSTMIMALEPVFIMLGAYILYKDRSTXXXXXXXXXXXFGVV 147 HF W+ + +T+VA + + + L+PV L ++++Y +R + GVV Sbjct: 67 FLAMHFFFWVSAFNHTTVAGAVIPLTLQPVLTGLLSWLVYGERLSPFRIVTGSMVVAGVV 126 Query: 148 LIGWGDIGLSSDNLKGDLLSVGGTVTVAGHLLIGQKLVARMPSYLYSLIVFITAAVVFAI 207 L G G S + +GDLL+V G V G+L++G+ L M S +SL A+ V A Sbjct: 127 LALAGKEGFSGIS-RGDLLAVIGVVFFCGYLVLGRYLNRAMGSMNFSLKTHALASTVLAF 185 Query: 208 YNLLAGIP-FLDYPPREWGIFVLLAVVPTVFGHILFNWLLQFTSATTVSMNILGEPVGAS 266 +P F REW I + L V + G++L N L++ ++ V + ++GEPV + Sbjct: 186 L-----VPSFRVVEAREWFILIGLGVGCSFLGYLLINLSLKYLPSSVVGVVLVGEPVLSI 240 Query: 267 ILAFLLLGEQLT 278 + +FLLLGE +T Sbjct: 241 LWSFLLLGETVT 252 >STM1787 [C] COG0374 Ni,Fe-hydrogenase I large subunit Length = 597 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 11/252 (4%), Positives = 43/252 (17%), Gaps = 11/252 (4%) Query: 28 IVAISFSAIFIKWSAAPASVQGMYRLLFTSLLMLPFARPYSGAAFALRRKDWXXXXXXXX 87 V + + + + ++ + F Sbjct: 11 TVNDAGRRLIVDPITRIEGHMRCEVNIDEQNVITNAVSCGT--MFRGLEI--ILQGRDPR 66 Query: 88 XXXXHFLLWMGSLKYTSVASSTMIMALEPVFIMLGAYILYKDRSTXXXXXXXXXXXFGVV 147 G +S + + + ++ F + Sbjct: 67 DAWAFVERICGVCTGVHALASVYAIEDAIGIQVPDNANIIRNIMLATLWCHDHLVHFYQL 126 Query: 148 LIGWGDIGLSSDNLKGDLLSVGGTVTVAGHLLIGQKLVARMPSYLYSLIVFITAAVVFAI 207 L++ + +++ + + + L + + Sbjct: 127 AGMDWIDVLNALK-ADPRATSQLAQSLSAWPMSSPGYFFDVQNRLKKFVDGGQLGIFRNG 185 Query: 208 YNLLAGIP-FLDYPPREWGIFVLLAVVPTVFGHILFNWLLQFTSATTVSMNILGEPVGAS 266 Y E + + + + N + + + Sbjct: 186 Y-----WGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTIFGGKNPHPNWIVGGMPCA 240 Query: 267 ILAFLLLGEQLT 278 I Sbjct: 241 INLDQSGAVGAI 252 >APE2537 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 311 Score = 71.2 bits (173), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 65/193 (33%), Positives = 103/193 (53%), Gaps = 6/193 (3%) Query: 92 HFLLWMGSLKYTSVASSTMIMALEPVFIMLGAYILYKDRSTXXXXXXXXXXXFGVVLIG- 150 HF LWM SL + SVA S ++ P + + + L+K++ T GV I Sbjct: 82 HFDLWMASLFHMSVAMSVTVVDSYPAVLAIVGFRLFKEKYTPLQLLGAVIAMVGVAGIAF 141 Query: 151 WGDIGLSS----DNLKGDLLSVGGTVTVAGHLLIGQKLVARMPSYLYSLIVFITAAVV-F 205 + G S+ D +KG LL+ G + VA + IG+ L ++ + Y+L V+ AA+V Sbjct: 142 YSSRGASAPPGGDPIKGFLLAFAGMLAVAAYFSIGKGLRSKYSTLEYTLPVYGFAAIVSL 201 Query: 206 AIYNLLAGIPFLDYPPREWGIFVLLAVVPTVFGHILFNWLLQFTSATTVSMNILGEPVGA 265 + L+ P Y + +LLA++P + GH L N LL+ S ++ ILGEPVGA Sbjct: 202 LLTTLVVRAPLTGYTAETYLYLILLALLPMLGGHTLINLLLRRLSLLAATVPILGEPVGA 261 Query: 266 SILAFLLLGEQLT 278 + LA++LLGE+++ Sbjct: 262 AALAWILLGEKVS 274 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.139 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,819,778 Number of extensions: 575513 Number of successful extensions: 1739 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 1734 Number of HSP's successfully gapped: 15 Length of query: 314 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 208 Effective length of database: 26,153,027 Effective search space: 5439829616 Effective search space used: 5439829616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 116 (49.3 bits)