BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0050.p (159 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3465 [Q] COG1335 Amidases related to nicotinamidase 107 8e-24 SP1745 [Q] COG1335 Amidases related to nicotinamidase 94 1e-19 PA1677 [Q] COG1335 Amidases related to nicotinamidase 82 3e-16 BS_yddQ [Q] COG1335 Amidases related to nicotinamidase 77 8e-15 lin0638 [Q] COG1335 Amidases related to nicotinamidase 77 1e-14 >CAC3465 [Q] COG1335 Amidases related to nicotinamidase Length = 178 Score = 107 bits (267), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 52/150 (34%), Positives = 89/150 (59%), Gaps = 3/150 (2%) Query: 10 HDEEGVMDRIISLLHKARTAGTPVVYIQHT--EDQEYTKGTPTWEISSHVTPQEGELIIE 67 ++ + V++ I L+ AR VVY++H E E KGT W+I S + P+ GE IIE Sbjct: 21 YNHKNVIENIKKLIIMARANNKEVVYVRHDDGEGTELEKGTEGWQIYSELEPKSGEFIIE 80 Query: 68 KPTWDAFHLTRLHDELQKRGITDLVICGMQSEFCVDTTTRRAFSMGYSSVLVEDAHSTFD 127 K AFH T L + L+ + +++ G+Q+E+C+D T + AF Y ++ +D ++TFD Sbjct: 81 KKYNSAFHRTDLKEYLESKNTDTIILVGLQTEYCIDATLKSAFDNEYKVIIPKDTNTTFD 140 Query: 128 NGSLSGGEIVKYHN-SVLGGRFAKLRTAQE 156 N LSG ++ K++N + RFA++ + ++ Sbjct: 141 NEYLSGEKLYKFYNYKIWNKRFARVISMED 170 >SP1745 [Q] COG1335 Amidases related to nicotinamidase Length = 166 Score = 93.6 bits (231), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 44/137 (32%), Positives = 80/137 (58%), Gaps = 1/137 (0%) Query: 13 EGVMDRIISLLHKARTAGTPVVYIQHTEDQEYTKGTPTWEISSHVTPQEGELIIEKPTWD 72 + ++++I L ++AR+ ++Y+QH E+ E + + W++S+ + + E + +K Sbjct: 22 KSLLEKISYLQNQARSKNIEIIYVQHIENSE-AQTSEDWQLSALLNRKPAEKVFQKKYNS 80 Query: 73 AFHLTRLHDELQKRGITDLVICGMQSEFCVDTTTRRAFSMGYSSVLVEDAHSTFDNGSLS 132 F T L + L K+GI LV+CGMQ+E+CVDT+ + AF GY ++ E A +TFD + Sbjct: 81 IFKETGLKEYLDKQGIEKLVLCGMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIP 140 Query: 133 GGEIVKYHNSVLGGRFA 149 I +++ + RFA Sbjct: 141 AETINEFYEDIWEERFA 157 >PA1677 [Q] COG1335 Amidases related to nicotinamidase Length = 198 Score = 82.0 bits (201), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 52/160 (32%), Positives = 79/160 (49%), Gaps = 8/160 (5%) Query: 7 MRLHDEEGVMDRIISLLHKARTAGTPVVYIQHTEDQE--YTKGTPTWEISSHVTPQEGEL 64 +RL + I L+ AR +GTP+V+++H Q + P + + P+ GE Sbjct: 39 LRLPGLDAAAAEIGVLVQAARASGTPIVHVRHLGIQGGLFDPQGPRGQFLPELQPEAGEK 98 Query: 65 IIEKPTWDAFHLTRLHDELQKRGITDLVICGMQSEFCVDTTTRRAFSMGYSSVLVEDAHS 124 ++EK +AF T LHD LQ G DL++CG + V TT R A GY L + A + Sbjct: 99 VVEKRLPNAFSGTELHDLLQNHGRLDLIVCGFMTHSSVSTTVRAAKDYGYRCTLADSACA 158 Query: 125 TFDNGSLSGG-----EIVKYHNSVLGGRFAKLRTAQEVEF 159 T D +L+GG ++ + + LG FA + AQ E Sbjct: 159 TRDIPTLNGGVLSAADLQRAEIAALGDNFAAI-VAQAREL 197 >BS_yddQ [Q] COG1335 Amidases related to nicotinamidase Length = 180 Score = 77.4 bits (189), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 45/128 (35%), Positives = 62/128 (48%), Gaps = 4/128 (3%) Query: 2 FSYPDMRLHDEEGVMDRIISLLHKARTAGTPVVYIQHTEDQE----YTKGTPTWEISSHV 57 F M L + E LL R G PV ++QH + + T EI V Sbjct: 15 FPNGKMALTNPEKAAQNAAKLLSHFRNTGAPVFHVQHITEGNIAHFFHPNTEGVEIHESV 74 Query: 58 TPQEGELIIEKPTWDAFHLTRLHDELQKRGITDLVICGMQSEFCVDTTTRRAFSMGYSSV 117 P E E +I K ++F T L+ +LQ+ G+ +LV+CGM S C+D T R A GY Sbjct: 75 RPLEKETVIVKHMPNSFFNTDLNGKLQEEGVKELVVCGMMSHMCIDATVRSAVEHGYVCQ 134 Query: 118 LVEDAHST 125 +VEDA +T Sbjct: 135 VVEDACAT 142 >lin0638 [Q] COG1335 Amidases related to nicotinamidase Length = 175 Score = 76.6 bits (187), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 41/136 (30%), Positives = 72/136 (52%), Gaps = 2/136 (1%) Query: 8 RLHDEEGVMDRIISLLHKARTAGTPVVYIQHTE--DQEYTKGTPTWEISSHVTPQEGELI 65 L++ + ++ I L+ AR ++Y++H + D E T G ++I P + E I Sbjct: 16 NLYNFDSLVSNIERLITNARKNKIEIIYVRHDDGPDTELTNGKEGFDIYEKFQPTKDERI 75 Query: 66 IEKPTWDAFHLTRLHDELQKRGITDLVICGMQSEFCVDTTTRRAFSMGYSSVLVEDAHST 125 +K AF T L L+++G +V+ G+Q++FC+D T + F G+ ++ A+ST Sbjct: 76 FDKTVNSAFKGTGLLAYLKEKGTKKIVVAGLQTDFCIDATVKCGFEHGFQMIVPAHANST 135 Query: 126 FDNGSLSGGEIVKYHN 141 DN +SG KY+N Sbjct: 136 EDNHFMSGENSYKYYN 151 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,162,699 Number of extensions: 528369 Number of successful extensions: 859 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 844 Number of HSP's successfully gapped: 39 Length of query: 159 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 61 Effective length of database: 27,075,699 Effective search space: 1651617639 Effective search space used: 1651617639 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)