BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0034.p (613 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2013_2 [T] COG0642 Signal transduction histidine kinase 221 4e-57 ZbarA_1 [T] COG0642 Signal transduction histidine kinase 206 1e-52 barA_1 [T] COG0642 Signal transduction histidine kinase 206 1e-52 ZbarA_1 [T] COG0642 Signal transduction histidine kinase 206 1e-52 all0638_1 [T] COG0642 Signal transduction histidine kinase 186 9e-47 >MA2013_2 [T] COG0642 Signal transduction histidine kinase Length = 296 Score = 221 bits (562), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 112/252 (44%), Positives = 161/252 (63%), Gaps = 11/252 (4%) Query: 344 QQELLIAKNAAESAARMKSEFLATMSHEIRTPLNGIIGINQLLAEEITAPDHQELLKLQN 403 +++LL AK AAESA + K +FLA MSHEIRTP+N ++G+ ++L E + +E L+L + Sbjct: 4 EEQLLKAKEAAESANKAKGDFLANMSHEIRTPMNAVLGMLEMLLETSLTDEQREYLQLAH 63 Query: 404 TSSLALLSVMNDVLDISRLEAENMLLVKAPFKLPALLRECINLFAVAAGHKGLALNLEIA 463 S+ +LLS+++DVLD S++E + L + F+L +L+ INL + AG KGL L I Sbjct: 64 ASAESLLSIIDDVLDFSKIEQNKLELEQISFELESLISHIINLLSGKAGSKGLKLAFHIE 123 Query: 464 PSIPEQLIGDSARIRQILVNLIGNAVKFTHEGSITVTIE---PCSKEG--------TSLG 512 +P IGD R++Q+L N++GNA+KFT +G I +++E P + + Sbjct: 124 KDLPSTFIGDPVRLKQVLFNILGNAIKFTKKGEIALSVEVYMPSERNSGLSNSDFPEEVA 183 Query: 513 LLFRVSDTGIGIAPDKLELLFQPFSQVDASHNRKYQGTGLGLAICKKLVELMDGSIWTEK 572 LLF+V DTGIGI P+KL +F PF Q DAS R+Y GTGLGLAI +LVELM+G IW E Sbjct: 184 LLFKVKDTGIGIPPEKLSQIFDPFIQADASVTREYGGTGLGLAISSQLVELMEGRIWVES 243 Query: 573 AREGGTDFFFKI 584 G F+F + Sbjct: 244 EVGKGCTFYFTV 255 >ZbarA_1 [T] COG0642 Signal transduction histidine kinase Length = 525 Score = 206 bits (523), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 110/256 (42%), Positives = 155/256 (60%) Query: 333 IAEDITALAEQQQELLIAKNAAESAARMKSEFLATMSHEIRTPLNGIIGINQLLAEEITA 392 + E + + Q EL +AK A+ AAR+KSEFLA MSHE+RTPLNG+IG +L + Sbjct: 265 LRETLEQMEIQNVELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELT 324 Query: 393 PDHQELLKLQNTSSLALLSVMNDVLDISRLEAENMLLVKAPFKLPALLRECINLFAVAAG 452 P ++ L S+ LL+++NDVLD S+LEA ++L PF L + L E + L A ++ Sbjct: 325 PTQRDHLNTIERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSH 384 Query: 453 HKGLALNLEIAPSIPEQLIGDSARIRQILVNLIGNAVKFTHEGSITVTIEPCSKEGTSLG 512 KGL L L I +P+ +IGD R++QI+ NL+GNA+KFT G+I + +E + T + Sbjct: 385 DKGLELTLNIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQ 444 Query: 513 LLFRVSDTGIGIAPDKLELLFQPFSQVDASHNRKYQGTGLGLAICKKLVELMDGSIWTEK 572 + ++ DTGIGI LFQ F Q DAS +R++ GTGLGL I +KLV M G I Sbjct: 445 IEVQIRDTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHS 504 Query: 573 AREGGTDFFFKIPLQL 588 G+ F+F I L L Sbjct: 505 QPNRGSTFWFHINLDL 520 >barA_1 [T] COG0642 Signal transduction histidine kinase Length = 525 Score = 206 bits (523), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 110/256 (42%), Positives = 155/256 (60%) Query: 333 IAEDITALAEQQQELLIAKNAAESAARMKSEFLATMSHEIRTPLNGIIGINQLLAEEITA 392 + E + + Q EL +AK A+ AAR+KSEFLA MSHE+RTPLNG+IG +L + Sbjct: 265 LRETLEQMEIQNVELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELT 324 Query: 393 PDHQELLKLQNTSSLALLSVMNDVLDISRLEAENMLLVKAPFKLPALLRECINLFAVAAG 452 P ++ L S+ LL+++NDVLD S+LEA ++L PF L + L E + L A ++ Sbjct: 325 PTQRDHLNTIERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSH 384 Query: 453 HKGLALNLEIAPSIPEQLIGDSARIRQILVNLIGNAVKFTHEGSITVTIEPCSKEGTSLG 512 KGL L L I +P+ +IGD R++QI+ NL+GNA+KFT G+I + +E + T + Sbjct: 385 DKGLELTLNIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQ 444 Query: 513 LLFRVSDTGIGIAPDKLELLFQPFSQVDASHNRKYQGTGLGLAICKKLVELMDGSIWTEK 572 + ++ DTGIGI LFQ F Q DAS +R++ GTGLGL I +KLV M G I Sbjct: 445 IEVQIRDTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHS 504 Query: 573 AREGGTDFFFKIPLQL 588 G+ F+F I L L Sbjct: 505 QPNRGSTFWFHINLDL 520 Score = 206 bits (523), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 110/256 (42%), Positives = 155/256 (60%) Query: 333 IAEDITALAEQQQELLIAKNAAESAARMKSEFLATMSHEIRTPLNGIIGINQLLAEEITA 392 + E + + Q EL +AK A+ AAR+KSEFLA MSHE+RTPLNG+IG +L + Sbjct: 265 LRETLEQMEIQNVELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELT 324 Query: 393 PDHQELLKLQNTSSLALLSVMNDVLDISRLEAENMLLVKAPFKLPALLRECINLFAVAAG 452 P ++ L S+ LL+++NDVLD S+LEA ++L PF L + L E + L A ++ Sbjct: 325 PTQRDHLNTIERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSH 384 Query: 453 HKGLALNLEIAPSIPEQLIGDSARIRQILVNLIGNAVKFTHEGSITVTIEPCSKEGTSLG 512 KGL L L I +P+ +IGD R++QI+ NL+GNA+KFT G+I + +E + T + Sbjct: 385 DKGLELTLNIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQ 444 Query: 513 LLFRVSDTGIGIAPDKLELLFQPFSQVDASHNRKYQGTGLGLAICKKLVELMDGSIWTEK 572 + ++ DTGIGI LFQ F Q DAS +R++ GTGLGL I +KLV M G I Sbjct: 445 IEVQIRDTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHS 504 Query: 573 AREGGTDFFFKIPLQL 588 G+ F+F I L L Sbjct: 505 QPNRGSTFWFHINLDL 520 >all0638_1 [T] COG0642 Signal transduction histidine kinase Length = 615 Score = 186 bits (472), Expect = 9e-47, Method: Compositional matrix adjust. Identities = 102/270 (37%), Positives = 158/270 (58%), Gaps = 4/270 (1%) Query: 330 VYIIAEDITALAEQQQELLIAKNAAESAARMKSEFLATMSHEIRTPLNGIIGINQLLAEE 389 VY+ + + T L + + AK +A+SA KSEFLA MSHE+RTPLNGI+G Q+L Sbjct: 333 VYVQSAEQTQLKQSKALADAAKESADSANNAKSEFLANMSHELRTPLNGILGYAQILGRS 392 Query: 390 ITAPDHQ-ELLKLQNTSSLALLSVMNDVLDISRLEAENMLLVKAPFKLPALLRECINLFA 448 PD + + + + + LL+++ND+LD+S++EA + L F LP+ ++ + + Sbjct: 393 KVLPDKERQGVNIIHQCGSHLLTLINDILDLSKIEARKLELASQAFHLPSFMQGVVEICH 452 Query: 449 VAAGHKGLALNLEIAPSIPEQLIGDSARIRQILVNLIGNAVKFTHEGSITVTIEPCSKEG 508 + KG+ + E +P + D R+RQ+L+NL+GNA+KFT GS+ + ++ E Sbjct: 453 IRVQQKGIEFHYEPDADLPAGISADEKRLRQVLINLLGNAIKFTDRGSVALRVQRTGLEE 512 Query: 509 TS--LGLLFRVSDTGIGIAPDKLELLFQPFSQVDASHNRKYQGTGLGLAICKKLVELMDG 566 + L F + DTG+GIA + LFQ F QV RK +GTGLGLAI ++LV+LM G Sbjct: 513 SDPRTKLRFSIVDTGVGIASADINKLFQTFEQV-GEQKRKVEGTGLGLAISQQLVQLMGG 571 Query: 567 SIWTEKAREGGTDFFFKIPLQLMDTIPRQE 596 I + G++FFF+I L L +Q+ Sbjct: 572 QIQVKSQLGVGSEFFFEIILPLASDWSQQQ 601 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,709,801 Number of extensions: 1546581 Number of successful extensions: 3894 Number of sequences better than 1.0e-05: 228 Number of HSP's gapped: 3512 Number of HSP's successfully gapped: 229 Length of query: 613 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 501 Effective length of database: 25,461,023 Effective search space: 12755972523 Effective search space used: 12755972523 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)