BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0032.p (107 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1422 [R] COG1253 Hemolysins and related proteins containing C... 79 2e-15 BS_yrkA [R] COG1253 Hemolysins and related proteins containing C... 75 2e-14 BS_yhdP [R] COG1253 Hemolysins and related proteins containing C... 70 6e-13 BB0059 [R] COG1253 Hemolysins and related proteins containing CB... 70 7e-13 BS_yqhB [R] COG1253 Hemolysins and related proteins containing C... 65 2e-11 >CAC1422 [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 428 Score = 78.6 bits (192), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 39/95 (41%), Positives = 64/95 (67%), Gaps = 1/95 (1%) Query: 1 MEEIVGEIQDEFDEERPGVEKLGEDEFSIDGLMLIEEINDRLGLNMETDDYDTIGGWLYS 60 +EEIVGEIQDEFDEE + K + +DG +LIE+IN+ L + + ++ DTIGGW+YS Sbjct: 332 LEEIVGEIQDEFDEEMEEIIKTENGSYVVDGKVLIEDINELLDIEIYEENIDTIGGWIYS 391 Query: 61 KLEVNPPQKGQTVEFDSHLFVVEETDNKRISRIKV 95 KL+ P+ + + + ++ F++ + D +RI +I + Sbjct: 392 KLK-KYPKTNEKINYGNYQFIILKCDRRRIEKILI 425 >BS_yrkA [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 434 Score = 75.5 bits (184), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 37/98 (37%), Positives = 67/98 (68%), Gaps = 3/98 (3%) Query: 1 MEEIVGEIQDEFDE-ERPGVEKLGEDEFSIDGLMLIEEINDRLGLNMETDDYDTIGGWLY 59 +EEIVGEI+DEFD+ E P + K+ ++ + +D +LIE++ND LG + +D+ DTIGGW Sbjct: 336 LEEIVGEIRDEFDKDEVPNIRKVNDNHYILDSKVLIEDVNDLLGTTLASDEVDTIGGWFM 395 Query: 60 SKLEVNPPQKGQTVEFDSHLFVVEETDNKRISRIKVMK 97 ++ +++ G +E D ++F V ET + I+ +++++ Sbjct: 396 TQ-QIDAAV-GSVIEADGYIFKVHETVGRHINYLEIVR 431 >BS_yhdP [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 444 Score = 70.5 bits (171), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 41/101 (40%), Positives = 68/101 (67%), Gaps = 5/101 (4%) Query: 1 MEEIVGEIQDEFD-EERPGVEKLGEDEFSIDGLMLIEEINDRLGLNMETDDYDTIGGW-L 58 +EEIVGEI+DEFD +E + K+GE + +DG +LI+++ND LG+++E ++ DTIGGW L Sbjct: 334 IEEIVGEIRDEFDIDEISEIRKIGEGHYILDGKVLIDQVNDLLGIHLENEEVDTIGGWFL 393 Query: 59 YSKLEVNPPQKGQTVEFDSHLFVVEETDNKRISRIKVMKVQ 99 K +V +K ++ + F++ E D ++ I+V K+Q Sbjct: 394 TQKYDV---EKDDSIIEEGCEFIINEIDGHHVAYIEVKKLQ 431 >BB0059 [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 259 Score = 70.1 bits (170), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 36/95 (37%), Positives = 61/95 (64%), Gaps = 1/95 (1%) Query: 1 MEEIVGEIQDEFDEERPGVEKLGEDEFSIDGLMLIEEINDRLGLNMETDDYDTIGGWLYS 60 +EEIVG+IQDEFD E + +L + + D +LIE++N++L LN+ D+DT+GG++Y Sbjct: 160 LEEIVGDIQDEFDNELDEIVRLDDGSYLCDARILIEDLNEKLNLNLPNGDFDTLGGFVYD 219 Query: 61 KLEVNPPQKGQTVEFDSHLFVVEETDNKRISRIKV 95 L P K + VE+++ +F ++ + I IK+ Sbjct: 220 -LFGRIPLKNEKVEYNNLVFSIKNMHQRNIKVIKI 253 >BS_yqhB [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 294 Score = 65.1 bits (157), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 0/100 (0%), Positives = 0/100 (0%), Gaps = 3/100 (3%) Query: 1 MEEIVGEIQDEFD-EERPGVEKLGEDEFSIDGLMLIEEINDRLGLNMETDDYDTIGGWLY 59 Sbjct: 345 404 Query: 60 SKLEVNPPQKGQTVEFDSHLFVVEETDNKRISRIKVMKVQ 99 Sbjct: 405 -- 442 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.137 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 8,404,058 Number of extensions: 343086 Number of successful extensions: 591 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 585 Number of HSP's successfully gapped: 32 Length of query: 107 Length of database: 38,378,431 Length adjustment: 75 Effective length of query: 32 Effective length of database: 29,728,381 Effective search space: 951308192 Effective search space used: 951308192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 109 (46.6 bits)