BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0031.p (341 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2334 [R] COG1253 Hemolysins and related proteins containing C... 285 9e-77 CAC1422 [R] COG1253 Hemolysins and related proteins containing C... 277 2e-74 BS_yrkA [R] COG1253 Hemolysins and related proteins containing C... 274 2e-73 BS_yhdT [R] COG1253 Hemolysins and related proteins containing C... 268 9e-72 BS_yugS [R] COG1253 Hemolysins and related proteins containing C... 261 1e-69 >lin2334 [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 434 Score = 285 bits (728), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 141/310 (45%), Positives = 214/310 (69%), Gaps = 9/310 (2%) Query: 21 NGFFVSVEYAMVXXXVRSGRIDSLIEEGSKRAVAAGNIVHNLDGYLSACQLGITLASLAL 80 + FFV+ E+A+V +R R+D LI E KRAV A +I ++L+ YLSACQLGIT++SL L Sbjct: 17 SAFFVATEFAIVK--MRPSRLDQLIAEKDKRAVLARHIYNHLNAYLSACQLGITISSLGL 74 Query: 81 GWLGEPAVATIVGPIVTSLGFGETSVYVISLIIAFMFITVLHIVLGELAPKTIAVNKAEA 140 GWLGE V + P+ + L ++++ ++S IAF+FIT LH+V+GEL PKT+A++K E Sbjct: 75 GWLGESTVEAALHPLFSLLELPQSAITILSFTIAFLFITFLHVVVGELVPKTLAIDKTET 134 Query: 141 VLLLTAGAMNVFFRIMYPIIWIVKGLASGLLRIFRLAPASELATAHTEEEIRIIMQESNK 200 V L A +++F+++M+P IWI+ G A + R+F L PASE AHTE+E++II+ ES K Sbjct: 135 VALAVARPLHIFYKVMFPFIWILNGSAVFIARLFGLEPASEHEIAHTEDELKIIVGESYK 194 Query: 201 SGLIDNTEMTLVDNIFEFADTMAREIMIPRTEMICLNNHLTVEENMEVAFDGMRTRYPVC 260 SG I+ +E V+ IF+F + MA+E+MIPRTE++ ++ T+ E ++ + TRYPV Sbjct: 195 SGEINQSEFRYVNKIFDFDERMAKEVMIPRTEIVTVDTGSTIGELSDIMQNERYTRYPVI 254 Query: 261 DGDKDHILGFIHIKDLI-----RDNAPKYS--ELIRPILTVPESIQISALLKVMQRAKTQ 313 DGDKDH++G +++K+++ + P +S ++PI+ V E+I I LL MQR ++ Sbjct: 255 DGDKDHVIGVLNLKEILSAYVEHGSNPSFSIDPYVKPIIRVIETIPIKELLFRMQRERSH 314 Query: 314 IAILIDEYGG 323 IAIL+DEYGG Sbjct: 315 IAILLDEYGG 324 >CAC1422 [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 428 Score = 277 bits (708), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 138/307 (44%), Positives = 203/307 (66%), Gaps = 6/307 (1%) Query: 21 NGFFVSVEYAMVXXXVRSGRIDSLIEEGSKRAVAAGNIVHNLDGYLSACQLGITLASLAL 80 N FFV+ E++MV +R RI++L+ +G K A A +++NL+ YLSACQLGITLASL L Sbjct: 17 NAFFVAAEFSMVK--IRKSRIETLVLDGDKNAKYALGVINNLNSYLSACQLGITLASLGL 74 Query: 81 GWLGEPAVATIVGPIVTSLGFGETSVYVISLIIAFMFITVLHIVLGELAPKTIAVNKAEA 140 GW+GEPAV+ ++ P+ L + + IS+++ F+ IT HIVLGELAPK++A+ E+ Sbjct: 75 GWVGEPAVSKMLSPLFNLLKIPKQIIGSISVVVGFIIITGFHIVLGELAPKSVAILNTES 134 Query: 141 VLLLTAGAMNVFFRIMYPIIWIVKGLASGLLRIFRLAPASELATAHTEEEIRIIMQESNK 200 + TA + +F++ YPII +L++F + E AHT+EEIR++++ES K Sbjct: 135 IAKATAIPLTLFYKFTYPIIVAFNKSTDLVLKLFGIKQVDEHEEAHTDEEIRMLVEESYK 194 Query: 201 SGLIDNTEMTLVDNIFEFADTMAREIMIPRTEMICLNNHLTVEENMEVAFDGMRTRYPVC 260 GLID TE+T VDN+F+F++ +EIM+PRT+M C+ + ++ + V D TRYPVC Sbjct: 195 HGLIDKTELTFVDNVFDFSEKTVKEIMVPRTDMKCIFVEDSFDDIVGVTIDEHFTRYPVC 254 Query: 261 DGDKDHILGFIHIKDL----IRDNAPKYSELIRPILTVPESIQISALLKVMQRAKTQIAI 316 + KD+I+G IHIKDL I K +IR I VPES+ IS L ++ Q+ + Q+AI Sbjct: 255 EESKDNIIGLIHIKDLYKLKINKEDEKIESIIREIKFVPESMSISELFRMFQKERMQMAI 314 Query: 317 LIDEYGG 323 +IDEYGG Sbjct: 315 VIDEYGG 321 >BS_yrkA [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 434 Score = 274 bits (700), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 141/311 (45%), Positives = 205/311 (65%), Gaps = 11/311 (3%) Query: 22 GFFVSVEYAMVXXXVRSGRIDSLIEEGSKRAVAAGNIVHNLDGYLSACQLGITLASLALG 81 FFV+ E+A+V +RS +ID LI EG K A++A ++ +LD YLSACQLGIT+ +L +G Sbjct: 17 AFFVATEFAIVK--IRSSKIDQLILEGKKGAISAKKVITHLDEYLSACQLGITVTALGIG 74 Query: 82 WLGEPAVATIVGPIVTSLGFGETSVYVISLIIAFMFITVLHIVLGELAPKTIAVNKAEAV 141 W+GE I+ P+ ET +V+ L+IAF+ T LH+V+GELAPKT+A+ KAE + Sbjct: 75 WVGESTFEVILHPLFAHFHVSETVSHVLILVIAFVMATFLHVVVGELAPKTLAIQKAETI 134 Query: 142 LLLTAGAMNVFFRIMYPIIWIVKGLASGLLRIFRLAPASELATAHTEEEIRIIMQESNKS 201 LLTA + F+RI++P IW + G A ++ +F L PASE AH+EEE+RI++ ES KS Sbjct: 135 TLLTAKPIIWFYRILFPFIWFLNGSARFIVGLFGLKPASEHELAHSEEELRILLSESYKS 194 Query: 202 GLIDNTEMTLVDNIFEFADTMAREIMIPRTEMICLNNHLTVEENMEVAFDGMRTRYPVCD 261 G I+ E+ V+NIFEF + +A+EIMIPR E++ +++ + E +++ TRYPV + Sbjct: 195 GEINQNELKYVNNIFEFDERIAKEIMIPRREIVAISSEDSYETIVKIIKTESYTRYPVLN 254 Query: 262 GDKDHILGFIHIKDLIR---DNAPKYSE------LIRPILTVPESIQISALLKVMQRAKT 312 GDKD I+GFI+ K+ + D K E I P++ V ES+ I +L MQ+ +T Sbjct: 255 GDKDSIIGFINAKEFLSAYIDTDQKIKEDFKLENHINPVIHVIESVPIHDVLVKMQKERT 314 Query: 313 QIAILIDEYGG 323 IAIL+DEYGG Sbjct: 315 HIAILVDEYGG 325 >BS_yhdT [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 80 Score = 268 bits (685), Expect = 9e-72, Method: Compositional matrix adjust. Identities = 0/312 (0%), Positives = 0/312 (0%), Gaps = 11/312 (3%) Query: 21 NGFFVSVEYAMVXXXVRSGRIDSLIEEGSKRAVAAGNIVHNLDGYLSACQLGITLASLAL 80 + + +V + + A + L L + + L Sbjct: 17 GLTLLYLAVWLVA--AYLSGVAPGFTGFPRWFEMACILTPLLFIGLCWAMVKFIYRDIPL 74 Query: 81 GWLGEPAVATIVGPIVTSLGFGETSVYVISLIIAFMFITVLHIVLGELAPKTIAVNKAEA 140 Sbjct: 75 134 Query: 141 VLLLTAGAMNVFFRIMYPIIWIVKGLASGLLRIFRLAPASELATAHTEEEIRIIMQESNK 200 Sbjct: 135 194 Query: 201 SGLIDNTEMTLVDNIFEFADTMAREIMIPRTEMICLNNHLTVEENMEVAFDGMRTRYPVC 260 Sbjct: 195 254 Query: 261 DGDKDHILGFIHIKDLIRDNAPKYSE---------LIRPILTVPESIQISALLKVMQRAK 311 Sbjct: 255 314 Query: 312 TQIAILIDEYGG 323 Sbjct: 315 326 >BS_yugS [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 434 Score = 261 bits (667), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 133/304 (43%), Positives = 196/304 (64%), Gaps = 6/304 (1%) Query: 24 FVSVEYAMVXXXVRSGRIDSLIEEGSKRAVAAGNIVHNLDGYLSACQLGITLASLALGWL 83 FV+ E+A+V +R +I+ LIE G RA+AA I+ NLD YLSACQLGIT+ +L LGWL Sbjct: 24 FVAAEFAIVK--IRGSKINQLIESGDSRALAAHKIISNLDEYLSACQLGITITALGLGWL 81 Query: 84 GEPAVATIVGPIVTSLGFGETSVYVISLIIAFMFITVLHIVLGELAPKTIAVNKAEAVLL 143 GEP + P+ T G E ++++ ++AF+ +T LH+V+GELAPKT+++ KAEAV L Sbjct: 82 GEPTFERFLHPLFTMTGIPEPFNHIVTFVVAFIIVTFLHVVMGELAPKTVSIQKAEAVSL 141 Query: 144 LTAGAMNVFFRIMYPIIWIVKGLASGLLRIFRLAPASELATAHTEEEIRIIMQESNKSGL 203 A + F++I YP I + G AS L+++F E +EEE+R+I+ ES + G Sbjct: 142 WIAKPLIWFYKITYPFIKALNGSASFLVKLFGFHSVKEHQVVISEEELRLILSESYEKGE 201 Query: 204 IDNTEMTLVDNIFEFADTMAREIMIPRTEMICLNNHLTVEENMEVAFDGMRTRYPVCDGD 263 I+ +E V+ IFEF + +AREIMIPRTE+ ++ ++EE + + TRYPV D Sbjct: 202 INQSEFRYVNKIFEFDNRVAREIMIPRTEIAVISLEQSLEEAIHHIINERYTRYPVIKDD 261 Query: 264 KDHILGFIHIKDLIR----DNAPKYSELIRPILTVPESIQISALLKVMQRAKTQIAILID 319 KDHILG I+ KD+ + K ++++RP++ V ESI + LL MQ+ + +AIL+D Sbjct: 262 KDHILGIINSKDMFKAYFLGQPIKLNQIMRPVIRVIESIPVQQLLIRMQKERIHMAILVD 321 Query: 320 EYGG 323 EYGG Sbjct: 322 EYGG 325 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.139 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,085,770 Number of extensions: 827703 Number of successful extensions: 3045 Number of sequences better than 1.0e-05: 73 Number of HSP's gapped: 2924 Number of HSP's successfully gapped: 73 Length of query: 341 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 234 Effective length of database: 26,037,693 Effective search space: 6092820162 Effective search space used: 6092820162 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 116 (49.3 bits)