BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0024.p (259 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM0019 [IQR] COG1028 Dehydrogenases with different specificities... 166 4e-41 STM2445 [IQR] COG1028 Dehydrogenases with different specificitie... 166 4e-41 STM0325 [L] COG2801 Transposase and inactivated derivatives 159 5e-39 TM0325 [IQR] COG1028 Dehydrogenases with different specificities... 159 5e-39 SMa0059 [IQR] COG1028 Dehydrogenases with different specificitie... 153 3e-37 >TM0019 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 256 Score = 166 bits (419), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 100/252 (39%), Positives = 149/252 (59%), Gaps = 4/252 (1%) Query: 10 LTGKKAIVTGAARGIGYEVARRFAEEGAAVVMADIDAE-GNHSAQQLQRAGLAVTFCRTD 68 L GK A+VTG +GIG +A+ FAE G VV+A+ID E G + L+ GL VTF +TD Sbjct: 2 LEGKVAVVTGGGQGIGAAIAQLFAENGMKVVIAEIDEEAGVEREEMLRERGLDVTFVKTD 61 Query: 69 VASNDSVVQLVDTAMELHGGIDILVNNAAVNIPGSILELSEEIWDRTYQVNVKSMFLLSR 128 VA +SV +V +E++GG+D+LVNNAAV SI E E W+R +VN+ ++ SR Sbjct: 62 VADENSVKNMVRKTVEIYGGVDVLVNNAAVMSVKSIFERPLEEWERVIRVNLTGPYICSR 121 Query: 129 AVIPIMQRSGSGAIVNMGSANSYVAEPRLSAYVSSKGAILMLSKAMALDFAADGIRVNCI 188 M + G G I+N+ S ++ +EP Y +SKG ++ L+ ++A+ + IRV I Sbjct: 122 YCAEEMIKRGGGVIINIASTRAFQSEPDTEPYSASKGGLVALTHSLAVSLSRYHIRVVSI 181 Query: 189 CPGWVDTAFNDSHAALFGGREEILKSIHDIHPIGRTIQPVEIANTALFLVSDE-ASAITG 247 PGW++T+ + + ++ H+ HP GR P++IA+ +FL DE A ITG Sbjct: 182 SPGWIETS--EWKKKSLRKKPDLRPIDHEQHPAGRVGNPLDIAHLCVFLADDEKAGFITG 239 Query: 248 AGILVDGGYTIK 259 +VDGG T+K Sbjct: 240 TNFIVDGGMTVK 251 >STM2445 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 263 Score = 166 bits (419), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 103/253 (40%), Positives = 147/253 (58%), Gaps = 3/253 (1%) Query: 9 RLTGKKAIVTGAARGIGYEVARRFAEEGAAVVMADIDAEGNHSAQQLQRAGLAVTFCRTD 68 +LTGK A++TGA++GIG +AR FA GA +++ DI E A +L G T + D Sbjct: 3 KLTGKTALITGASQGIGEGIARVFARHGANLILLDISDEIEKLADELGGRGHRCTAVKAD 62 Query: 69 VASNDSVVQLVDTAMELHGGIDILVNNAAVNIPGSILELSEEIWDRTYQVNVKSMFLLSR 128 V SV V A E G IDILVNNA V G+ L++SEE D +N+K ++ +++ Sbjct: 63 VRDFASVQAAVARAKETEGRIDILVNNAGVCRLGNFLDMSEEDRDFHIDINIKGVWNVTK 122 Query: 129 AVIPIMQRSGSGAIVNMGSAN-SYVAEPRLSAYVSSKGAILMLSKAMALDFAADGIRVNC 187 AV+P M + G IV M S VA+P +AY SK AI+ L+K++A+++A GIRVN Sbjct: 123 AVLPEMIKRKDGRIVMMSSVTGDMVADPGETAYALSKAAIVGLTKSLAVEYAQSGIRVNA 182 Query: 188 ICPGWVDTAFNDSHAALFG--GREEILKSIHDIHPIGRTIQPVEIANTALFLVSDEASAI 245 ICPG+V T +S A E +L + P+ R P+E+ A FL SDE+S + Sbjct: 183 ICPGYVRTPMAESIARQSNPDDPESVLTEMAKAIPLRRLADPLEVGELAAFLASDESSYL 242 Query: 246 TGAGILVDGGYTI 258 TG ++DGG T+ Sbjct: 243 TGTQNVIDGGSTL 255 >STM0325 [L] COG2801 Transposase and inactivated derivatives Length = 266 Score = 159 bits (401), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 14/251 (5%), Positives = 37/251 (14%), Gaps = 3/251 (1%) Query: 8 MRLTGKKAIVTGAARGIGYEVARRFAEEGAAVVMADIDAE-GNHSAQQLQRAGLAVTFCR 66 M + R + + A + + Sbjct: 1 MLASTPPTSTGVFNRALSSLAIACICKRYVWHQRRHQINRRQRFHLVCDNVFREAFSDAK 60 Query: 67 TDVASNDSVVQLVDTAMELHGGIDILVNNAAVNIPGSILELSEEIWDRTYQVNVKSMFLL 126 + A + + + + S LL Sbjct: 61 QRYGAPRL--TDELRAQGYQFNVKTVAASLRRQGLRAKASRRFRPVSYRKHGLPVSENLL 118 Query: 127 SRAVIPIMQRSGSGAIVNMGSANSYVAEPRLSAYVSSKGAILMLSKAMALDFAADGIRVN 186 + + + + S+ I + A Sbjct: 119 KQDFYASGPNQKWVGDITYLRTGEGWLYLAVVIDLWSRSVIGWSMSSRMTAQLACDALQM 178 Query: 187 CICPGWVDTAFNDSHAALFGGREEILKSIHDIHPIGRTIQPVEIANTALFLVSDEASAIT 246 + +S+ H + ++ S + Sbjct: 179 ALWRRKCPENVIVHTDRGGQYCSTDYQSLLKRHNLRGSMSARGCCYDNACAESFFHTLKV 238 Query: 247 GAGILVDGGYT 257 D Sbjct: 239 ECIHGEDFVSR 249 >TM0325 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 251 Score = 159 bits (401), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 98/251 (39%), Positives = 137/251 (54%), Gaps = 3/251 (1%) Query: 8 MRLTGKKAIVTGAARGIGYEVARRFAEEGAAVVMADIDAE-GNHSAQQLQRAGLAVTFCR 66 M GK ++TGA GIG + A FAE GA V + DI E G + + ++ G F Sbjct: 1 MNFQGKVVLITGAGSGIGKKAAVMFAERGAKVAINDISEEKGKETVELIKSMGGEAAFIF 60 Query: 67 TDVASNDSVVQLVDTAMELHGGIDILVNNAAVNIPGSILELSEEIWDRTYQVNVKSMFLL 126 DVA + Q+V +E G +DILVNNA + G+I E SEE +D+T VNVK FLL Sbjct: 61 GDVAKDAE--QIVKKTVETFGRLDILVNNAGIVPYGNIEETSEEDFDKTMAVNVKGPFLL 118 Query: 127 SRAVIPIMQRSGSGAIVNMGSANSYVAEPRLSAYVSSKGAILMLSKAMALDFAADGIRVN 186 S+ + M++ G G IVN+ S + PR Y SK A+L L++++A+D+ GIRVN Sbjct: 119 SKYAVEQMKKQGGGVIVNVSSEAGLIGIPRRCVYSVSKAALLGLTRSLAVDYVDYGIRVN 178 Query: 187 CICPGWVDTAFNDSHAALFGGREEILKSIHDIHPIGRTIQPVEIANTALFLVSDEASAIT 246 +CPG + + EE+LK + P+ R + EIA LF DEA +T Sbjct: 179 AVCPGTTQSEGLMARVKASPNPEELLKKMTSRIPMKRLGKEEEIAFAILFAACDEAGFMT 238 Query: 247 GAGILVDGGYT 257 G+ I +DGG T Sbjct: 239 GSIINIDGGST 249 >SMa0059 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 261 Score = 153 bits (386), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 95/256 (37%), Positives = 140/256 (54%), Gaps = 11/256 (4%) Query: 10 LTGKKAIVTGAARGIGYEVARRFAEEGAAV-VMADIDAEGNHSAQQL--QRAGLAVTFCR 66 GK +VTG + G+G A RFA G V ++A+ A + + + AG Sbjct: 4 FNGKSIVVTGGSLGMGLACAHRFAAGGGKVTIVANDKASVDEAVTSIGDNAAGFV----- 58 Query: 67 TDVASNDSVVQLVDTAMELHGGIDILVNNAAVNIPGSILELSEEIWDRTYQVNVKSMFLL 126 DV S + V A+ HGG+DIL A + G++++ ++E+WD +N+K +FL Sbjct: 59 GDVRSKADMNAAVQAAVSRHGGVDILACCAGIQRYGTVVDTADEVWDDVLDINLKGIFLA 118 Query: 127 SRAVIPIMQRSGSGAIVNMGSANSYVAEPRLSAYVSSKGAILMLSKAMALDFAADGIRVN 186 S+ IP M++ G GAIV + S +Y ++ ++AY +SKGAI L +AMALD A D I VN Sbjct: 119 SKFAIPEMRKRGGGAIVAISSVQAYASQTGVAAYTASKGAINALVRAMALDHAGDNITVN 178 Query: 187 CICPGWVDTAFNDSHAALFGGR---EEILKSIHDIHPIGRTIQPVEIANTALFLVSDEAS 243 +CP +DT A L+ G E L++ HP+GR +P E+A FL S++A Sbjct: 179 AVCPASIDTPMLRWAADLWKGEGTVEATLETWGKGHPLGRVGKPSEVAELVAFLASEKAR 238 Query: 244 AITGAGILVDGGYTIK 259 ITGA I +DGG K Sbjct: 239 FITGADIKIDGGVLSK 254 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,698,382 Number of extensions: 671718 Number of successful extensions: 3226 Number of sequences better than 1.0e-05: 368 Number of HSP's gapped: 2538 Number of HSP's successfully gapped: 368 Length of query: 259 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 155 Effective length of database: 26,383,695 Effective search space: 4089472725 Effective search space used: 4089472725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)