BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0022.p (374 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yqiT [E] COG0334 Glutamate dehydrogenase/leucine dehydrogenase 575 e-164 BH2765 [E] COG0334 Glutamate dehydrogenase/leucine dehydrogenase 540 e-153 all0426 [E] COG0334 Glutamate dehydrogenase/leucine dehydrogenase 356 3e-98 BH0218 [E] COG0334 Glutamate dehydrogenase/leucine dehydrogenase 310 3e-84 PA3418 [E] COG0334 Glutamate dehydrogenase/leucine dehydrogenase 290 3e-78 >BS_yqiT [E] COG0334 Glutamate dehydrogenase/leucine dehydrogenase Length = 252 Score = 575 bits (1481), Expect = e-164, Method: Compositional matrix adjust. Identities = 0/364 (0%), Positives = 0/364 (0%) Query: 6 MNLLETMQQSDYEEVVFCQDRQSGLKAIIAIHDTTLGPALGGTRMWAYASEEAALTDVLR 65 M+ + + + + + G A + + Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 66 LSRGMTYKNAVSGLNLGGGKAVIIGNPKADKNEAMFRAFGRYIQGLNGRYITAEDVGTTE 125 + +Q L Y + + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 126 DDMNLIHEETEYVTGVSETYGSSGNPSPATAYGVYQGMKAAALEAFGTDSLEGRTVAVQG 185 + ++ + + A + S G V + Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 186 VGNVAYHLCSYLHQEGARLIVTDIDKEAVRRALEAYGAKAVSPDDIYSVACDIYAPCALG 245 + + + + + K L Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 246 ATVNDQTISQLKARVIAGAANNQLQEARHGDLLHELGIVYAPDYVLNAGGVIHIADELNG 305 Sbjct: 241 300 Query: 306 YNRERAYKKVGEIYRNIRRVFEISAEQGIPSYLAADRLAEERIAGLHASRRTFLQNEQHA 365 Sbjct: 301 360 Query: 366 LSRR 369 Sbjct: 361 364 >BH2765 [E] COG0334 Glutamate dehydrogenase/leucine dehydrogenase Length = 252 Score = 540 bits (1390), Expect = e-153, Method: Compositional matrix adjust. Identities = 0/364 (0%), Positives = 0/364 (0%) Query: 6 MNLLETMQQSDYEEVVFCQDRQSGLKAIIAIHDTTLGPALGGTRMWAYASEEAALTDVLR 65 M+ + + + + + G A + + Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 66 LSRGMTYKNAVSGLNLGGGKAVIIGNPKADKNEAMFRAFGRYIQGLNGRYITAEDVGTTE 125 + +Q L Y + + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 126 DDMNLIHEETEYVTGVSETYGSSGNPSPATAYGVYQGMKAAALEAFGTDSLEGRTVAVQG 185 + ++ + + A + S G V + Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 186 VGNVAYHLCSYLHQEGARLIVTDIDKEAVRRALEAYGAKAVSPDDIYSVACDIYAPCALG 245 + + + + + K L Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 246 ATVNDQTISQLKARVIAGAANNQLQEARHGDLLHELGIVYAPDYVLNAGGVIHIADELNG 305 Sbjct: 241 300 Query: 306 YNRERAYKKVGEIYRNIRRVFEISAEQGIPSYLAADRLAEERIAGLHASRRTFLQNEQHA 365 Sbjct: 301 360 Query: 366 LSRR 369 Sbjct: 361 364 >all0426 [E] COG0334 Glutamate dehydrogenase/leucine dehydrogenase Length = 252 Score = 356 bits (914), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 0/344 (0%), Positives = 0/344 (0%), Gaps = 5/344 (1%) Query: 6 MNLLETMQQSDYEEVVFCQDRQSGLKAIIAIHDTTLGPALGGTRMWAYASEEAALTDVLR 65 M+ + + + + + G A + + Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 66 LSRGMTYKNAVSGLNLGGGKAVIIGNPKADKNEAMFRAFGRYIQGLNGRYITAEDVGTTE 125 + +A+ F + + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNW-QPVQALDLIFANASLQWLPDHYELFPHLVSL 119 Query: 126 DDMNLIHEETEYVTGVSETYGSSGNPSPATAYGVYQGMKAAALEAFGTDSLEGRTVAVQG 185 + + + + Y L + Sbjct: 120 LNPQGVLAVQMPDNWLEP---THVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEV 176 Query: 186 VGNVAYHLCSYLHQEGARLIVTDIDKEAVRRALEAYGAKAVSPDDIYSVACDIYAPCALG 245 + + VT + L + + Sbjct: 177 DIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQ 236 Query: 246 ATVNDQTISQLKARVIAGAANNQLQEAR-HGDLLHELGIVYAPDYVLNAGGVIHIADELN 304 Sbjct: 237 296 Query: 305 GYNRERAYKKVGEIYRNIRRVFEISAEQGIPSYLAADRLAEERI 348 Sbjct: 297 340 >BH0218 [E] COG0334 Glutamate dehydrogenase/leucine dehydrogenase Length = 252 Score = 310 bits (793), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 0/347 (0%), Positives = 0/347 (0%), Gaps = 9/347 (2%) Query: 4 SAMNLLETMQQSDYEEVVFCQDRQSGLKAIIAIHDTTLGPALGGTRMWAYASEEAALTDV 63 + + E + A + L A + + + Sbjct: 10 LHFSAERSR--PAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAM 67 Query: 64 LRLSRGMTYKNAVSGLNLGGGKAVIIGNPKADKNEAMFRAFGRYIQGLNGRYITAEDVGT 123 + +R ++ + V + + + + + V Sbjct: 68 IAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLA 127 Query: 124 TEDDMNLIHEETEYVTGVSETYGSSGNPSPATAYGVYQGMKAAALEAFGTDSLEGRTVAV 183 + N + + V+ A + + Sbjct: 128 VQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQM 187 Query: 184 QGVGNVAYHLCSYLHQEGARLIVTDIDKEAVRRALEAYGAK------AVSPDDIYSVACD 237 + + + + + + ++ + + + Sbjct: 188 PSHQAIIDWVTATGLRPWLQDLTESE-QQLFLKRYHQMLEEQYPLQENGQILLAFPRLFI 246 Query: 238 IYAPCALGATVNDQTISQLKARVIAGAANNQLQEARHGDLLHELGIVYAPDYVLNAGGVI 297 Sbjct: 247 306 Query: 298 HIADELNGYNRERAYKKVGEIYRNIRRVFEISAEQGIPSYLAADRLA 344 Sbjct: 307 353 >PA3418 [E] COG0334 Glutamate dehydrogenase/leucine dehydrogenase Length = 252 Score = 290 bits (742), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 0/344 (0%), Positives = 0/344 (0%), Gaps = 6/344 (1%) Query: 8 LLETMQQSDYEEVVFCQDRQSGLKAIIAIHDTTLGPALGGTRMWAYASEEAALTDVLRLS 67 + + L A + + + LG + A + Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 68 RGMTYKNAVSGLNLGGGKAVIIGNPKADKNEAMFRAFGRYIQGLNGRYITAEDVGTTEDD 127 + + + + + + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 128 MNLIHEETEYVTGVSETYGSSGNPSPATAYGVYQGMKAAALEAFGTDSLEGRTVAVQGVG 187 + E + G + Sbjct: 121 NPQGVLAVQMPDNWLE----PTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEV 176 Query: 188 NVAYHLCSYLHQEGARLIVTDIDKEAVRRALEAYGAKAVSPDDIYSVACDIYAPCALGAT 247 ++ + +I + ++ Y + P Sbjct: 177 DIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQ 236 Query: 248 VNDQTISQLKARVIAGAANNQLQEARHGDLLHELGIVYAPDYVLNAGGVIHIADELNGYN 307 Sbjct: 237 296 Query: 308 RERAYKKVGEIYRNIRRVF-EISAEQGIPSYLAADRLAEERIAG 350 Sbjct: 297 - 339 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,459,626 Number of extensions: 1212588 Number of successful extensions: 4431 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 4357 Number of HSP's successfully gapped: 35 Length of query: 374 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 266 Effective length of database: 25,922,359 Effective search space: 6895347494 Effective search space used: 6895347494 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)