BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0021.p (442 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll1522 [GEPR] COG0477 Permeases of the major facilitator superf... 85 2e-16 >mll1522 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 297 Score = 85.1 bits (209), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 0/324 (0%), Positives = 0/324 (0%), Gaps = 8/324 (2%) Query: 106 PLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVSDRIGPVRILLCGIAATLSGSL 165 + + + L S Sbjct: 49 RVRKNESNNSHEIVLTVASLEYIWAFSNFFWVFTQEYSKSQKNNDEHFDLTGKNRLKKSD 108 Query: 166 CFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKANSKRQAAYLAALLVTAGNALG 225 L ++ + + A + L Sbjct: 109 ELLKWARKNLQTTGCESWPKKCPKPEAYLQGSEDSQVASEIFLCAIAWILHHEISHVVLQ 168 Query: 226 PVLSGILAEYTPWPTRMPFLVHLLLVVPAVIGLLLVRIR---SEPAGTASLHW-PR--VP 279 L +L + + Sbjct: 169 HPLVTTAFSTQEEREADSHATKWILGNLYES--APELKKRALGIATAVLCIQSLEVENYF 226 Query: 280 AGIRGAFYTASLTSFLVWGVVSLFMSVVPTYLGVWVDRTXXXXXXXXXXXXXXXXXXXQF 339 + ++ V + Sbjct: 227 CLQNTHPAAYERIYSNISCYPVGNEELIEALCTVMLQYLFHGKNINVNLDGESFSSILGD 286 Query: 340 TLGKLPLLRMMTXXXXXXXXXXXXXXXXXRFHSLIILFASILLVGMGHGPLYAGSLAYVN 399 Sbjct: 287 346 Query: 400 AIAPSEARGDTVSFFYTGTYIGVA 423 Sbjct: 347 370 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,773,147 Number of extensions: 991566 Number of successful extensions: 5234 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 5274 Number of HSP's successfully gapped: 14 Length of query: 442 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 333 Effective length of database: 25,807,025 Effective search space: 8593739325 Effective search space used: 8593739325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)