BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0017.p (452 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Rv2860c [E] COG0174 Glutamine synthetase 373 e-103 MT2928 [E] COG0174 Glutamine synthetase 373 e-103 BMEII0554 [E] COG0174 Glutamine synthetase 325 6e-89 AGc3883 [E] COG0174 Glutamine synthetase 323 3e-88 mll3074 [E] COG0174 Glutamine synthetase 321 2e-87 >Rv2860c [E] COG0174 Glutamine synthetase Length = 252 Score = 373 bits (958), Expect = e-103, Method: Compositional matrix adjust. Identities = 0/450 (0%), Positives = 0/450 (0%), Gaps = 6/450 (1%) Query: 4 LEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIED-AKDGIHTCALNLAWDVS 62 R + E+ + + G + ++ + + Sbjct: 9 YLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMI 68 Query: 63 LN-FGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRNML 121 A C + L S + +L + ++ + Sbjct: 69 AEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAV 128 Query: 122 KRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHW 181 + + + + Y + Y + VD Sbjct: 129 QMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMP 188 Query: 182 FLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGL 241 + D + A G Q+ L + E + + Sbjct: 189 SHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLAFPRLFIVA 248 Query: 242 MATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPEL 301 Sbjct: 249 -- 306 Query: 302 MVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGADANHYL 361 Sbjct: 307 - 365 Query: 362 VLSAMIASGLYGIENRLELPAPITGNAGK-LEIPRLPTNLYDAVKILENSKTVRELLGEE 420 Sbjct: 366 425 Query: 421 VVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 Sbjct: 426 455 >MT2928 [E] COG0174 Glutamine synthetase Length = 252 Score = 373 bits (958), Expect = e-103, Method: Compositional matrix adjust. Identities = 0/450 (0%), Positives = 0/450 (0%), Gaps = 6/450 (1%) Query: 4 LEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIED-AKDGIHTCALNLAWDVS 62 R + E+ + + G + ++ + + Sbjct: 9 YLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMI 68 Query: 63 LN-FGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRNML 121 A C + L S + +L + ++ + Sbjct: 69 AEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAV 128 Query: 122 KRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHW 181 + + + + Y + Y + VD Sbjct: 129 QMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMP 188 Query: 182 FLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGL 241 + D + A G Q+ L + E + + Sbjct: 189 SHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLAFPRLFIVA 248 Query: 242 MATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPEL 301 Sbjct: 249 -- 306 Query: 302 MVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGADANHYL 361 Sbjct: 307 - 365 Query: 362 VLSAMIASGLYGIENRLELPAPITGNAGK-LEIPRLPTNLYDAVKILENSKTVRELLGEE 420 Sbjct: 366 425 Query: 421 VVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 Sbjct: 426 455 >BMEII0554 [E] COG0174 Glutamine synthetase Length = 252 Score = 325 bits (834), Expect = 6e-89, Method: Compositional matrix adjust. Identities = 0/428 (0%), Positives = 0/428 (0%), Gaps = 4/428 (0%) Query: 26 VDMQGKLFGKRVPGHHFIEDAKDGIHTCALNLAWDVSLN-FGAFDFCNWDTGVHDMVTLP 84 + +R + + + N + + + P Sbjct: 6 PSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSP 65 Query: 85 DLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRNMLKRQIAKAAGLGLKPYAASEIEFY 144 + + + + N + + L + + Sbjct: 66 AMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGV 125 Query: 145 IFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHWFLGQLTDNLEAAGGAIEALKGEW 204 + + + E + ++ + R + + I Sbjct: 126 LAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYH 185 Query: 205 GNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGLMATFMAKQDTNDSGSSGHTHISL 264 + + + A + +++ + Sbjct: 186 QMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLAFPRLF 245 Query: 265 WDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPELMVFYAPYINSYKRIMDFGSGAPN 324 Sbjct: 246 - 304 Query: 325 TVSWGIDNRSTSFRFDGKG-KSCRFENRVPGADANHYLVLSAMIASGLYGIENRLELPAP 383 Sbjct: 305 364 Query: 384 ITGNA-GKLEIPRLPTNLYDAVKILENSKTVRELLGEEVVHHYLQAAQLELRDFFSSVTD 442 Sbjct: 365 424 Query: 443 WERKKYFE 450 Sbjct: 425 432 >AGc3883 [E] COG0174 Glutamine synthetase Length = 211 Score = 323 bits (829), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 0/454 (0%), Positives = 0/454 (0%), Gaps = 6/454 (1%) Query: 1 MAYLEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIEDAKDGIHTCALNLAWD 60 M + ++ ++ +L + + + + +A Sbjct: 1 MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANT 60 Query: 61 VSL-NFGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSD-LKSKDGTDISIAPR 118 + + + + ++ + ++ + + Sbjct: 61 PAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRK 120 Query: 119 NMLKRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTV 178 + + E+ L + + + Sbjct: 121 TANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLI 180 Query: 179 DHWFLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAAL 238 Sbjct: 181 240 Query: 239 NGLMATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELA 298 Sbjct: 241 - 299 Query: 299 PELMVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDG-KGKSCRFENRVPGADA 357 Sbjct: 300 - 358 Query: 358 NHYLVLSAMIASGLYGIENRLELPAPITGNA-GKLEIPRLPTNLYDAVKILENSKTVREL 416 Sbjct: 359 418 Query: 417 LGEEVVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 Sbjct: 419 452 >mll3074 [E] COG0174 Glutamine synthetase Length = 252 Score = 321 bits (822), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 0/451 (0%), Positives = 0/451 (0%), Gaps = 5/451 (1%) Query: 4 LEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIEDAKDGIHTCALNLAWDVSL 63 E ++ ++ + + D+ A+ + + + Sbjct: 14 AERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARS 73 Query: 64 N-FGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSD-LKSKDGTDISIAPRNML 121 + L + W P + + + + Sbjct: 74 ALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDN 133 Query: 122 KRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHW 181 + + + + + Y + Sbjct: 134 WLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAI 193 Query: 182 FLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGL 241 Sbjct: 194 253 Query: 242 MATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPEL 301 Sbjct: 254 313 Query: 302 MVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKG-KSCRFENRVPGADANHY 360 Sbjct: 314 - 372 Query: 361 LVLSAMIASGLYGIENRLELPAPITGNAGKL-EIPRLPTNLYDAVKILENSKTVRELLGE 419 Sbjct: 373 432 Query: 420 EVVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 Sbjct: 433 463 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,588,929 Number of extensions: 1479188 Number of successful extensions: 2710 Number of sequences better than 1.0e-05: 67 Number of HSP's gapped: 2453 Number of HSP's successfully gapped: 67 Length of query: 452 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 342 Effective length of database: 25,691,691 Effective search space: 8786558322 Effective search space used: 8786558322 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)