BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0016.p (253 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1921 [IQR] COG1028 Dehydrogenases with different specificitie... 186 3e-47 BS_fabG [IQR] COG1028 Dehydrogenases with different specificitie... 180 2e-45 YPO0839 [IQR] COG1028 Dehydrogenases with different specificitie... 175 6e-44 BS_gdh [IQR] COG1028 Dehydrogenases with different specificities... 171 7e-43 STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases... 169 4e-42 >lin1921 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 200 Score = 186 bits (472), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 0/257 (0%), Positives = 0/257 (0%), Gaps = 15/257 (5%) Query: 1 MRFADKVVIVTGAGSGNGRSIAMKFAEEGAKVVAADLNLAG----AEETVALIGK-GKEA 55 M G + ++ T+ L Sbjct: 1 MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLE---QAAYEMTALRAATIELGPLVRGLP 57 Query: 56 LALKSDVTLKSDVEHMVAQTIETYGTLDVLVNNAGIVAFTEFLELSEEEWDKVHNVNLKA 115 L D+ Q E E L ++ ++ + V L+ Sbjct: 58 HLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNALMQDLEEEGYLVGLEK 117 Query: 116 PFLCSQAAAKQMIASGTKGRIINITSVEAHRIVSSSGHCQPHYNSSKGGLNLLTKAVALE 175 + A + + + + + + + K + Sbjct: 118 AKFVERLAHYYCEIN-VLHPFRVGSGLAQ--RIFFEQ--LAIHAGYQLSWQGIEKEAWNQ 172 Query: 176 LAPYGITVNSVAPGVVETPFTAGGLQNPEVREWVLGRIPAGRIASPGDIANAVLFLATPE 235 G + A ++ + + Sbjct: 173 ANQSGAMGDLTALQMIFSKVVSE-- 230 Query: 236 SEYITGSTVFVDGGWTI 252 Sbjct: 231 247 >BS_fabG [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 180 bits (456), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 105/251 (41%), Positives = 156/251 (62%), Gaps = 11/251 (4%) Query: 5 DKVVIVTGAGSGNGRSIAMKFAEEGAKVVAA-DLNLAGAEETVALI-GKGKEALALKSDV 62 DK IVTGA G GRSIA+ A+ GA VV N A A E V I G++A+A+K+DV Sbjct: 4 DKTAIVTGASRGIGRSIALDLAKSGANVVVNYSGNEAKANEVVDEIKSMGRKAIAVKADV 63 Query: 63 TLKSDVEHMVAQTIETYGTLDVLVNNAGIVAFTEFLELSEEEWDKVHNVNLKAPFLCSQA 122 + DV++M+ +T+ + T+D+LVNNAGI + + E+EWD V N+NLK F C++A Sbjct: 64 SNPEDVQNMIKETLSVFSTIDILVNNAGITRDNLIMRMKEDEWDDVININLKGVFNCTKA 123 Query: 123 AAKQMIASGTKGRIINITSVEAHRIVSSSGH-CQPHYNSSKGGLNLLTKAVALELAPYGI 181 +QM+ + GRIIN++S IV SG+ Q +Y ++K G+ LTK+ A ELA I Sbjct: 124 VTRQMMKQRS-GRIINVSS-----IVGVSGNPGQANYVAAKAGVIGLTKSSAKELASRNI 177 Query: 182 TVNSVAPGVVETPFTAGGLQNPEVREWVLGRIPAGRIASPGDIANAVLFLATPESEYITG 241 TVN++APG + T T + +V++ +L +IP R P D+++ V FLA+ + Y+TG Sbjct: 178 TVNAIAPGFISTDMTDKLAK--DVQDEMLKQIPLARFGEPSDVSSVVTFLASEGARYMTG 235 Query: 242 STVFVDGGWTI 252 T+ +DGG + Sbjct: 236 QTLHIDGGMVM 246 >YPO0839 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 251 Score = 175 bits (443), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 96/246 (39%), Positives = 148/246 (60%), Gaps = 7/246 (2%) Query: 6 KVVIVTGAGSGNGRSIAMKFAEEGAKVVAADLNLAGAEETVALI-GKGKEALALKSDVTL 64 KV I+TG +G G+ +A+ A+ GA +V + +A A ET A + G++ + +D+ Sbjct: 9 KVAIITGCNTGLGQGMAIGLAKAGADIVG--VGVAEAPETQAQVEALGRKFHFITADLIQ 66 Query: 65 KSDVEHMVAQTIETYGTLDVLVNNAGIVAFTEFLELSEEEWDKVHNVNLKAPFLCSQAAA 124 + D++ +V+Q +E G +D+L+NNAGI+ + LE ++WD V N+N K F SQA A Sbjct: 67 QKDIDSIVSQAVEVMGHIDILINNAGIIRRQDLLEFGNKDWDDVININQKTVFFLSQAVA 126 Query: 125 KQMIASGTKGRIINITSVEAHRIVSSSGHCQPHYNSSKGGLNLLTKAVALELAPYGITVN 184 KQ + G G+IINI S+ + + G P Y +SK + LT+A+A EL+ Y I VN Sbjct: 127 KQFVKQGNGGKIINIASMLSFQ----GGIRVPSYTASKSAVMGLTRALATELSQYNINVN 182 Query: 185 SVAPGVVETPFTAGGLQNPEVREWVLGRIPAGRIASPGDIANAVLFLATPESEYITGSTV 244 ++APG + T TA + E +L RIPA R +P D+A +FL++ S+Y+TG T+ Sbjct: 183 AIAPGYMATDNTAALRADTARNEAILERIPASRWGTPDDLAGPAIFLSSSASDYVTGYTL 242 Query: 245 FVDGGW 250 VDGGW Sbjct: 243 AVDGGW 248 >BS_gdh [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 261 Score = 171 bits (434), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 100/254 (39%), Positives = 150/254 (59%), Gaps = 14/254 (5%) Query: 6 KVVIVTGAGSGNGRSIAMKFAEEGAKVV------AADLNLAGAEETVALIGKGKEALALK 59 KVV +TGA SG G+++A++F +E AKVV D N E +I G EA+ ++ Sbjct: 8 KVVAITGAASGLGKAMAIRFGKEQAKVVINYYSNKQDPNEVKEE----VIKAGGEAVVVQ 63 Query: 60 SDVTLKSDVEHMVAQTIETYGTLDVLVNNAGIVAFTEFLELSEEEWDKVHNVNLKAPFLC 119 DVT + DV+++V I+ +GTLD+++NNAG+ E+ ++WDKV NL FL Sbjct: 64 GDVTKEEDVKNIVQTAIKEFGTLDIMINNAGLENPVPSHEMPLKDWDKVIGTNLTGAFLG 123 Query: 120 SQAAAKQMIASGTKGRIINITSVEAHRIVSSSGHCQPHYNSSKGGLNLLTKAVALELAPY 179 S+ A K + + KG +IN++SV H ++ HY +SKGG+ L+T+ +ALE AP Sbjct: 124 SREAIKYFVENDIKGNVINMSSV--HEVIPWPLFV--HYAASKGGIKLMTETLALEYAPK 179 Query: 180 GITVNSVAPGVVETPFTAGGLQNPEVREWVLGRIPAGRIASPGDIANAVLFLATPESEYI 239 GI VN++ PG + TP A +P+ + V IP G I P +IA +LA+ E+ Y+ Sbjct: 180 GIRVNNIGPGAINTPINAEKFADPKQKADVESMIPMGYIGEPEEIAAVAAWLASKEASYV 239 Query: 240 TGSTVFVDGGWTIH 253 TG T+F DGG T + Sbjct: 240 TGITLFADGGMTQY 253 >STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases component II Length = 197 Score = 169 bits (428), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 0/258 (0%), Positives = 0/258 (0%), Gaps = 18/258 (6%) Query: 1 MRFADKVVIVTGAGSGNGRSIAMKFAEEGAKVVAADLNLAGAEETVALIGKGKEALALKS 60 M + + + L L L Sbjct: 1 MADILLLDNIDSFTWNLADQLRTNGHNVVIYRNHIPA----QTLIDRLATMKNPVLMLSP 56 Query: 61 D-----VTLKSDVEHMVAQTIETYGTLDVLVNNAGIVAFTEFLELSEEEWDKVHNVNLKA 115 + + + + E K ++ Sbjct: 57 GPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDG 116 Query: 116 PFLCSQAAAKQMIASGTKGRIINITSVEAHRIVSSSGHCQPHYNSSKGGLNLLTKAVALE 175 + + A +A + + +++ + + + + Sbjct: 117 QAMFAGLANPLPVAR-YHSLVGSNVPAGL--TINAHF--NGMVMAVRHDADRVCGFQFHP 171 Query: 176 LAPYGITVNSVAPGVVETPFTAGGLQNPE-VREWVLGRIPAGRIASPGDIANAVLFLATP 234 + + + P Sbjct: 172 ESILTTQGARLLEQTLAWAQQK---LEPT 228 Query: 235 ESEYITGSTVFVDGGWTI 252 Sbjct: 229 246 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.131 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,255,096 Number of extensions: 767720 Number of successful extensions: 3509 Number of sequences better than 1.0e-05: 342 Number of HSP's gapped: 2827 Number of HSP's successfully gapped: 343 Length of query: 253 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 149 Effective length of database: 26,383,695 Effective search space: 3931170555 Effective search space used: 3931170555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)