BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0007.p (297 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0267 [C] COG0039 Malate/lactate dehydrogenases 294 1e-79 BH3937 [C] COG0039 Malate/lactate dehydrogenases 290 1e-78 BS_lctE [C] COG0039 Malate/lactate dehydrogenases 279 3e-75 SPAC186.08c [C] COG0039 Malate/lactate dehydrogenases 279 3e-75 SA0232 [C] COG0039 Malate/lactate dehydrogenases 276 4e-74 >CAC0267 [C] COG0039 Malate/lactate dehydrogenases Length = 313 Score = 294 bits (753), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 139/283 (49%), Positives = 206/283 (72%), Gaps = 9/283 (3%) Query: 22 QELVLIDVNHQKALGEALDMNHGMPFVGGVKLWAGTYEDCREADIIIVTAGASQKPGETR 81 E+V++DVN KA GEA+D++HG FV V + +G Y+D +DI+I+TAGA+QKPGETR Sbjct: 30 SEIVIVDVNKDKAEGEAMDLSHGAAFVKPVVVKSGDYKDTEGSDIVIITAGAAQKPGETR 89 Query: 82 IDLLRKNISIFKDIIQKITKYNHHAILLIATNPVDILAYATLKISGFDRRRVIGSGTVLD 141 ++L+ KN +IFK I+ ++ KYN +AILL+ +NPVDIL Y T K+SGF + RVIGSGTVLD Sbjct: 90 LELINKNYNIFKSIVPEVVKYNPNAILLVVSNPVDILTYITYKLSGFPKSRVIGSGTVLD 149 Query: 142 SARFRYLIGLHKEIDPRSIHGQIIGEHGDSELPVWSLANVAGIDLG---------FDDAE 192 ++RFRY++ H EID R+IH I+GEHGDSE+ WSL N+AG+D+ D + Sbjct: 150 TSRFRYMLSEHFEIDVRNIHTYIMGEHGDSEIATWSLTNIAGMDVNEYCEASCKKCDGSL 209 Query: 193 RKEIFEDTKNAAYEIIDAKGSTSYAIALALDRIVVSILHNEGAVLNVSTLLNNYNGVSDV 252 + +I++D KNAAY +I+ KG+T YA+ALA+ RIV +IL +E ++L VS+LL G+ DV Sbjct: 210 KYKIYDDVKNAAYHVIEKKGATYYAVALAVKRIVEAILRDENSILTVSSLLEGQYGIKDV 269 Query: 253 FLGAPCVVDRSGVREVLDLPLSEDEKTLFQKSAEKLKSEISKL 295 ++G P +V +GV++++++PL+++EK SA+ LK + + Sbjct: 270 YMGVPSIVGINGVKDIIEVPLNDEEKNNLTDSAKTLKESLDSI 312 >BH3937 [C] COG0039 Malate/lactate dehydrogenases Length = 310 Score = 290 bits (743), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 140/270 (51%), Positives = 195/270 (72%), Gaps = 3/270 (1%) Query: 23 ELVLIDVNHQKALGEALDMNHGMPFVGG-VKLWAGTYEDCREADIIIVTAGASQKPGETR 81 EL+L+D+N +KA G+ LD+NH + + +++ G+YEDC++A ++++ AGA+QKPGETR Sbjct: 33 ELILVDLNEEKAKGDVLDLNHSIVYAPSPMEIKFGSYEDCKDAALVVICAGAAQKPGETR 92 Query: 82 IDLLRKNISIFKDIIQKITKYNHHAILLIATNPVDILAYATLKISGFDRRRVIGSGTVLD 141 +DL+ KN+ IF+ I+ I K + I L+ATNPVDILAYAT K SG + RVIGSGTVLD Sbjct: 93 LDLVHKNVGIFESIVGNIMKSGFNGIFLVATNPVDILAYATWKFSGLPKERVIGSGTVLD 152 Query: 142 SARFRYLIGLHKEIDPRSIHGQIIGEHGDSELPVWSLANVAGIDLG--FDDAERKEIFED 199 +ARFRYL+G P S+HG IIGEHGDS+LPVWS A +AG + D +++EI E+ Sbjct: 153 TARFRYLLGEEMNAAPTSVHGYIIGEHGDSQLPVWSSATIAGTPIAPRLTDEKKQEIAEN 212 Query: 200 TKNAAYEIIDAKGSTSYAIALALDRIVVSILHNEGAVLNVSTLLNNYNGVSDVFLGAPCV 259 ++AAY+II+AKG+T Y IA L RI +IL NE VL V TLL NG DV++G P + Sbjct: 213 VRDAAYKIIEAKGATYYGIATGLARITRAILKNENVVLPVGTLLEGENGHDDVYIGVPAI 272 Query: 260 VDRSGVREVLDLPLSEDEKTLFQKSAEKLK 289 ++R GVR+V++L L+++EK F +S E LK Sbjct: 273 INREGVRQVVELSLNDEEKEKFARSVETLK 302 >BS_lctE [C] COG0039 Malate/lactate dehydrogenases Length = 321 Score = 279 bits (714), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 138/277 (49%), Positives = 189/277 (68%), Gaps = 9/277 (3%) Query: 23 ELVLIDVNHQKALGEALDMNHGMPF-VGGVKLWAGTYEDCREADIIIVTAGASQKPGETR 81 ELV+IDVN +KA+G+ +D+ HG F + VK GTYEDC++ADI+ + AGA+QKPGETR Sbjct: 33 ELVVIDVNKEKAMGDVMDLPHGKAFGLQPVKTSYGTYEDCKDADIVCICAGANQKPGETR 92 Query: 82 IDLLRKNISIFKDIIQKITKYNHHAILLIATNPVDILAYATLKISGFDRRRVIGSGTVLD 141 ++L+ KN+ IFK I+ ++ I L+ATNPVDIL YAT K SG + RVIGSGT LD Sbjct: 93 LELVEKNLKIFKGIVSEVMASGFDGIFLVATNPVDILTYATWKFSGLPKERVIGSGTTLD 152 Query: 142 SARFRYLIGLHKEIDPRSIHGQIIGEHGDSELPVWSLANVAGIDL--------GFDDAER 193 SARFR+++ + P+++H IIGEHGD+ELPVWS ANV G+ + + E Sbjct: 153 SARFRFMLSEYFGAAPQNVHAHIIGEHGDTELPVWSHANVGGVPVSELVEKNDAYKQEEL 212 Query: 194 KEIFEDTKNAAYEIIDAKGSTSYAIALALDRIVVSILHNEGAVLNVSTLLNNYNGVSDVF 253 +I +D KNAAY II+ KG+T Y +A++L RI +ILHNE ++L VST L+ G DV+ Sbjct: 213 DQIVDDVKNAAYHIIEKKGATYYGVAMSLARITKAILHNENSILTVSTYLDGQYGADDVY 272 Query: 254 LGAPCVVDRSGVREVLDLPLSEDEKTLFQKSAEKLKS 290 +G P VV+R G+ + +L L+E EK F SA LK+ Sbjct: 273 IGVPAVVNRGGIAGITELNLNEKEKEQFLHSAGVLKN 309 >SPAC186.08c [C] COG0039 Malate/lactate dehydrogenases Length = 330 Score = 279 bits (714), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 135/284 (47%), Positives = 199/284 (70%), Gaps = 10/284 (3%) Query: 23 ELVLIDVNHQKALGEALDMNHGMPFVGGVKLWAGTYEDCREADIIIVTAGASQKPGETRI 82 E+V+ID+N +KA GEA+D+NH P +++ G Y+DC++A +++TAG +QKPGETR+ Sbjct: 47 EIVIIDLNKKKAEGEAMDLNHAAPLSHETRVYLGDYKDCKDATAVVITAGKNQKPGETRM 106 Query: 83 DLLRKNISIFKDIIQKITKYNHHAILLIATNPVDILAYATLKISGFDRRRVIGSGTVLDS 142 DLL+ NISIFK+I++++TKY AILL+ATNPVD+L YATLK++GF RVIGSGT++D+ Sbjct: 107 DLLKANISIFKEILREVTKYTKDAILLVATNPVDVLTYATLKLTGFPAERVIGSGTIIDT 166 Query: 143 ARFRYLIGLHKEIDPRSIHGQIIGEHGDSELPVWSLANVAGIDLG---------FDDAER 193 ARF+YLIG +DP+S++ IIGEHGDSEL VWS A++AG+ L +D+ Sbjct: 167 ARFQYLIGKLYGLDPQSVNADIIGEHGDSELAVWSHASIAGLSLADFCEESETKYDEQAL 226 Query: 194 KEIFEDTKNAAYEIIDAKGSTSYAIALALDRIVVSILHNEGAVLNVSTLLNNYNGVSDVF 253 E F++TKNAAY+II KGST Y +A L RI+ +I+ +E A+L VS L++Y+ + DV Sbjct: 227 NECFKETKNAAYDIIQRKGSTEYGVAAGLVRILAAIIRDENALLTVSG-LDSYSNIGDVC 285 Query: 254 LGAPCVVDRSGVREVLDLPLSEDEKTLFQKSAEKLKSEISKLEL 297 P +++ G +++ LS+DE +S + +K I + L Sbjct: 286 FSMPRKLNKDGAHRIINAKLSKDEDAKLVESVKSIKHAIESIGL 329 >SA0232 [C] COG0039 Malate/lactate dehydrogenases Length = 317 Score = 276 bits (705), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 136/284 (47%), Positives = 196/284 (69%), Gaps = 8/284 (2%) Query: 21 MQELVLIDVNHQKALGEALDMNHGMPFV-GGVKLWAGTYEDCREADIIIVTAGASQKPGE 79 + ELV+ID++ +K G+ +D+ H P+ V++ AG Y DC +AD++++ AGA+QKPGE Sbjct: 31 VDELVIIDLDTEKVRGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGE 90 Query: 80 TRIDLLRKNISIFKDIIQKITKYNHHAILLIATNPVDILAYATLKISGFDRRRVIGSGTV 139 TR+DL+ KN+ IFK I+ ++ I L+ATNPVDILAYAT K SG + RVIGSGT+ Sbjct: 91 TRLDLVSKNLKIFKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTI 150 Query: 140 LDSARFRYLIGLHKEIDPRSIHGQIIGEHGDSELPVWSLANVAGIDLGF-------DDAE 192 LDSARFR L+ ++ PRS+ QIIGEHGD+ELPVWS AN+AG L A+ Sbjct: 151 LDSARFRLLLSEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQ 210 Query: 193 RKEIFEDTKNAAYEIIDAKGSTSYAIALALDRIVVSILHNEGAVLNVSTLLNNYNGVSDV 252 ++IF T++AAY+II AKG+T Y +A+ L RI +I NE AVL VS LL DV Sbjct: 211 IEQIFVQTRDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDV 270 Query: 253 FLGAPCVVDRSGVREVLDLPLSEDEKTLFQKSAEKLKSEISKLE 296 ++G P V++R+G+R V+++PL+++E++ F SA+ LK +++ E Sbjct: 271 YIGVPAVINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAE 314 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,507,642 Number of extensions: 937577 Number of successful extensions: 3069 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 3029 Number of HSP's successfully gapped: 29 Length of query: 297 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 191 Effective length of database: 26,153,027 Effective search space: 4995228157 Effective search space used: 4995228157 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)