BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0004.p (426 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yecA [E] COG0531 Amino acid transporters 244 2e-64 YPO0655 [E] COG0531 Amino acid transporters 160 5e-39 VCA0847 [E] COG0531 Amino acid transporters 132 1e-30 CAC3285 [E] COG0531 Amino acid transporters 91 4e-18 VNG0174G_1 [E] COG0531 Amino acid transporters 86 2e-16 >BS_yecA [E] COG0531 Amino acid transporters Length = 221 Score = 244 bits (624), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 0/406 (0%), Positives = 0/406 (0%) Query: 3 QAIALYIGAVLGSGVLIVPGLAAEMAGPASLLAWGFMTLLILPLALSMGLLSARFPNAGG 62 + +L V L + S ++ + + Sbjct: 13 WLDDILTKYNTDHAILDVAELDGLLTAVLSSPQEIEPEQWLVAVWGGADYVPRWASEKEM 72 Query: 63 VSHFVTLAFGPKAGALVGWFFLMSVPIGGPVAALTGAGYMTAAMGWGDNARIAMAAAMLG 122 A F + + G+ R + Sbjct: 73 TRFMNLAFQHMADTAERLNEFPEQFEPLFGLREVDGSELTIVEEWCFGYMRGVALSDWST 132 Query: 123 IGLVTNWIGMQXXXXXXXXXXXXXXXXXXXXXXXXLPRMESAHFTPFAPHGWMSVGQAAA 182 + + + A A +A Sbjct: 133 LPDSLKPALEAIALHGTEENFERVEKMSPEAFEESVDAIRLAALDLHAYWMAHPQEKAVQ 192 Query: 183 ILFWCFIGWEAVSHLSEEFKDPQRAAVRGVTIAAIIVGVLYFLSALATVGTQSYLRGGAD 242 Sbjct: 193 252 Query: 243 SSLVWIISQPLGSWGGFIAGLTGLFICTATIIAYTGAASRVAFALSRQGYAPKWMGRLSS 302 Sbjct: 253 312 Query: 303 RYQTPTGAIGXXXXXXXXXXXXYGSGLLSITTLIQFPNATFILTYIGGCAAGIRLLKGNR 362 Sbjct: 313 372 Query: 363 LGVVISWISFTATAAVFPFTGWAIGYPLVITALFLLIMYTRTGRQN 408 Sbjct: 373 418 >YPO0655 [E] COG0531 Amino acid transporters Length = 416 Score = 160 bits (404), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 123/411 (29%), Positives = 193/411 (46%), Gaps = 25/411 (6%) Query: 1 MPQAIALYIGAVLGSGVLIVPGLAAEMAGPASLLAWGFMTLLILPLALSMGLLSARFPNA 60 + Q + L ++LG+GV VP LAA +AG SL AW + +L+ P+A++ L FP+A Sbjct: 10 LAQGVGLLSTSLLGTGVFAVPALAAMLAGHDSLWAWPVLIVLVFPIAIAFAALGRHFPSA 69 Query: 61 GGVSHFVTLAFGPKAGALVGWFFLMSVPIGGPVAALTGAGYMTAAMGWGDNARIAMAAAM 120 GG +HFV +AFGPK G + GW FL +P+G P A AG+ A GW D + + Sbjct: 70 GGAAHFVAIAFGPKLGKVTGWLFLSVIPVGLPAALQIAAGFWQAMFGWSDTGLLMVQL-- 127 Query: 121 LGIGLVTNWI----------GMQXXXXXXXXXXXXXXXXXXXXXXXXLPRMESAHFTPFA 170 + L+ W+ +Q +P +P Sbjct: 128 --VTLLVIWLLGTRSAGSSANLQTVIALLVIALVAAIWWQGDIRLSQIPWPALQDVSP-- 183 Query: 171 PHGWMSVGQAAAILFWCFIGWEAVSHLSEEFKDPQRAAVRGVTIAAIIVGVLYFLSALAT 230 P+ + A A++FWCF+G EA +HL+ EF+ P+R R + + ++ G +Y+ +A Sbjct: 184 PN----IFNALAVMFWCFVGLEAFAHLATEFRHPERDFPRALIVGLLVAGAVYWGCTVAV 239 Query: 231 VGTQSY-LRGGADSSLVWIISQPLGSWGGFIAGLTGLFICTATIIAYTGAASRVAFALSR 289 + Q+Y A +SL I+ Q G +IA + G C A++ YT + +R+ ++ S+ Sbjct: 240 LHFQAYGESQAAAASLPGIVVQLFGEHALWIACIIGYLACFASVNIYTQSFARMVWSQSQ 299 Query: 290 QGYAPKWMGRLSSRYQTPTGAIGXXXXXXXXXXXXYGSGLLSITTLIQFPNATFILTYIG 349 K + RLS+ QTP A+ L + LI + N F+L Y+ Sbjct: 300 VRPQSK-LARLSAG-QTPVNALTAVVGSCLVFALLIYWLALPLDLLIVYANGIFVLIYLL 357 Query: 350 GCAAGIRLLKGNRLGVVISWISFTATAAVFPFTGWAIGYPLVITALFLLIM 400 AGIRLL G V+S I + GW Y L++ AL ++M Sbjct: 358 CMLAGIRLLSGR--ARVMSVIGSLLCCVLLVMIGWKSVYALLMFALLWMLM 406 >VCA0847 [E] COG0531 Amino acid transporters Length = 440 Score = 132 bits (332), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 97/387 (25%), Positives = 175/387 (45%), Gaps = 4/387 (1%) Query: 12 VLGSGVLIVPGLAAEMAGPASLLAWGFMTLLILPLALSMGLLSARFPNAGGVSHFVTLAF 71 ++G+G+ +VP +AA +AG SL AW + + I P+AL+ L R+PNAGG ++FV AF Sbjct: 47 LMGTGLFMVPAIAAGIAGVFSLWAWLLLFVAICPIALTFAQLGKRYPNAGGTAYFVRQAF 106 Query: 72 GPKAGALVGWFFLMSVPIGGPVAALTGAGYMTAAMGWGDNARIAMAAAMLGIGLVTNWIG 131 P+ V W FL +P+G P A A ++ A + + + + + N G Sbjct: 107 NPRLERAVAWLFLSVIPVGVPAAVTLAASFLQALLPAPLANPLLTQTLTILLLVGVNLAG 166 Query: 132 MQXXXXXXXXXXXXXXXXXXXXXXXXLPRMESAHFTPFAPHGWMSVGQAAAILFWCFIGW 191 + + GW+S+G A A++FWCF+G Sbjct: 167 TKSSGRLQTLIALAIFSLVIAFWWRGEVTTQDLVMPALTQEGWVSIGAALAVMFWCFVGI 226 Query: 192 EAVSHLSEEFKDPQRAAVRGVTIAAIIVGVLYFLSALATVGTQSYLRGGADS-SLVWIIS 250 EA +H+ EEF++PQR + I + + G+ Y+ ++ + ++ DS ++ W+ Sbjct: 227 EAFAHMGEEFRNPQRDFPLAILIGSFVAGLTYWACSVVVLKFSAFGTPQFDSGAIPWLSD 286 Query: 251 QPLGSWGGFIAGLTGLFICTATIIAYTGAASRVAFALSRQGYAPKWMGRLSSRYQTPTGA 310 Q G+ + + G C A++ YT + +R+ +A +R+ + R+S R Sbjct: 287 QLFGTRWAIMISVIGFLACFASLNLYTQSLARMVWAQAREYRPESAIARVSIRGVPAHAT 346 Query: 311 IGXXXXXXXXXXXXYGSGLLSITTLIQFPNATFILTYIGGCAAGIRLLKGNRLGVVISWI 370 + + +G L + ++ N F+L Y+ A +LL G ++ + Sbjct: 347 LLVGVVLFASCFIGHLTG-LDLEFFLKLANGIFVLVYLLAMLAAFKLLTG--WSKHLAGL 403 Query: 371 SFTATAAVFPFTGWAIGYPLVITALFL 397 + A VF GW++ Y L + L + Sbjct: 404 ALVLCAGVFLCLGWSMLYALGVFVLLI 430 >CAC3285 [E] COG0531 Amino acid transporters Length = 466 Score = 90.5 bits (223), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 74/319 (23%), Positives = 147/319 (46%), Gaps = 20/319 (6%) Query: 6 ALYIGAVLGSGVLIVPGLAAEMAGPASLLAWGFMTLLILPLALSMGLLSARFPNAGGVSH 65 A+ IGAV+G+G+ + G A++AGP+ ++++ + +L+ LS+ F +G Sbjct: 32 AIGIGAVVGTGIFVSTGQGAKLAGPSVVISFLVAAVTCGLCSLTYCELSSMFSVSGSTYS 91 Query: 66 FVTLAFGPKAGALVGWFFLMSVPIGGPVAALTGAGYMTAAMG----WGDNARIAMAAAML 121 + +AFG ++GW ++ + A+ +G+ + +G +G N A+ + L Sbjct: 92 YSYIAFGEIIAWIIGWDLMLEYLVA---ASAISSGWSSTLIGIVKNYGVNVPDALTKSPL 148 Query: 122 G----------IGLVTNWI---GMQXXXXXXXXXXXXXXXXXXXXXXXXLPRMESAHFTP 168 I LV ++ G+ + ++ ++ P Sbjct: 149 SGGIVDLPAIFITLVITFLLYRGVTESAKINNVIVGVKICIIALFVFLGITHVKVTNYHP 208 Query: 169 FAPHGWMSVGQAAAILFWCFIGWEAVSHLSEEFKDPQRAAVRGVTIAAIIVGVLYFLSAL 228 F P+G + AAAI+F+ FIG++A++ +EE KDP++ +G+ I +V VLY A+ Sbjct: 209 FVPYGVNGIMSAAAIIFFSFIGFDAIATTAEETKDPKKDVPKGLLICFGVVVVLYMSVAI 268 Query: 229 ATVGTQSYLRGGADSSLVWIISQPLGSWGGFIAGLTGLFICTATIIAYTGAASRVAFALS 288 G Y + +++L +S +WG + G+ + +T++ R+ +S Sbjct: 269 ILTGIVPYTKIDINNALPGALSSIGITWGSALVGVGAVLGMISTLLVIMYGQIRIFMVMS 328 Query: 289 RQGYAPKWMGRLSSRYQTP 307 R G PK ++ ++ TP Sbjct: 329 RDGLLPKVFSSVNKKHSTP 347 >VNG0174G_1 [E] COG0531 Amino acid transporters Length = 563 Score = 85.5 bits (210), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 90/332 (27%), Positives = 145/332 (43%), Gaps = 39/332 (11%) Query: 13 LGSGVLIVPGLAAEMAGPASLLAWGFMTLLILPLALSMGLLSARFPNAGGVSHFVTLAFG 72 +G+G+ ++P +AAE AGPAS++++ ++ L ALS+ L+ P AGG ++V A G Sbjct: 2 IGAGIFVLPKIAAEQAGPASMVSFFVGGMVSLFAALSLSELATGMPKAGGSYYYVNRALG 61 Query: 73 PKAGALVGW-----------FFLMSVPIGGPVAALTGAG-YMTAAMGWGDNARIAMAAAM 120 P G++VGW F++ IG L G G Y+ GWGD A AM Sbjct: 62 PFFGSIVGWGMWAGLTFASAFYM----IGFGQYLLPGLGEYVGVLAGWGDLGITVAALAM 117 Query: 121 LGIGLVTNWIGMQXXXXXXXXXXXXXXXXXXXXXXXXLPRMESAHFTPFAPHGWMSVGQA 180 + N+ G++ L + T F P+GW +V Sbjct: 118 AALLTAVNYYGVK--ETSALQNVIVLTLVGLVLAFLGLGAVTGPTITEFNPNGWPAVAAT 175 Query: 181 AAILFWCFIGWEAVSHLSEEFKDPQRAAVRGVTIAAIIVGVLYFLSALATVGTQSY-LRG 239 ++ FIG+E ++ +EE K+P R + A + ++Y + GT + Sbjct: 176 IGTVYVTFIGFEVIATSAEEIKNPSRNLPLAMIAAVVTPTLMYVGVMFVSTGTIDIPVLA 235 Query: 240 GADSSLVWI---ISQPLG---SWGGFI------AGLTG-LFICTATIIAYTGAAS----- 281 G+D + + I QPL SW F+ AG G L + ++A +A+ Sbjct: 236 GSDVPVALVAREIFQPLAGAVSWLPFVSVQPATAGSVGALAMIVGAVLATVSSANASILS 295 Query: 282 --RVAFALSRQGYAPKWMGRLSSRYQTPTGAI 311 RV FA+ R W+ + R++TP AI Sbjct: 296 AARVNFAMGRDKILVNWLNEVHDRFRTPYRAI 327 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.140 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,123,790 Number of extensions: 1103697 Number of successful extensions: 6161 Number of sequences better than 1.0e-05: 81 Number of HSP's gapped: 6167 Number of HSP's successfully gapped: 95 Length of query: 426 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 317 Effective length of database: 25,807,025 Effective search space: 8180826925 Effective search space used: 8180826925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)