BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0521.0001.p (332 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0293 [V] COG1619 Uncharacterized proteins, homologs of microc... 103 3e-22 alr3273 [V] COG1619 Uncharacterized proteins, homologs of microc... 89 1e-17 VCA0439 [V] COG1619 Uncharacterized proteins, homologs of microc... 88 2e-17 VCA0337 [V] COG1619 Uncharacterized proteins, homologs of microc... 88 2e-17 alr2723 [V] COG1619 Uncharacterized proteins, homologs of microc... 65 1e-10 >CAC0293 [V] COG1619 Uncharacterized proteins, homologs of microcin C7 resistance protein MccF Length = 306 Score = 103 bits (257), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 82/296 (27%), Positives = 138/296 (46%), Gaps = 27/296 (9%) Query: 22 IKKGAAIGVTAASAGVGPQSVELFGQAITRMEEKGYHVLCGDTVFRHDKAKSASGLVRGK 81 +K G IG+ + S+ P+S+E +AI E +G+ + G V+ + R Sbjct: 6 LKTGDTIGIISPSSPENPKSIE---KAINFFENRGFKIKLGKHVYDKRGYLAGEDKDRAN 62 Query: 82 EFNQMMSSDEVDLILPPWGGELLIEMLEHIDFSQVKN--KWIMGFSDISLLLLVITLRTG 139 + M D VD+IL GG + L +IDF ++N K +GFSDI+ L ++G Sbjct: 63 DIMDMFKDDTVDMILCVRGGYGSMRTLPYIDFEVIRNNPKIFVGFSDITSFLNTFYSKSG 122 Query: 140 IATAHGPSLGDLRGEVIDKTT--AKWETVLSTASGESVIQRSSPTDWKTVSGEAVTLQGR 197 + T HGP DK T + + T+++ ++ SS V GEA +G Sbjct: 123 LVTFHGPM---FTSNFDDKYTIDSFFNTLMNGDVPYRILNPSSIELSCAVEGEA---KGH 176 Query: 198 LLGGCIDVIRHIIGTPYGDVRHFQENFIHQEPILWYLENCELNTADLRRSLVQMKLAGWF 257 L+GG + +I + +GTPY F+ N + ++E+ + R L ++L+G Sbjct: 177 LVGGNLSLISNTLGTPYE--IDFKNN-------ILFIEDVHEEPYAIDRMLTHIELSGKL 227 Query: 258 DNCSGVMFGRSGSNTPMENY----TAEDVYRELTEELQVPVVYDLDYGHIPPQMTL 309 CSG + G+ N + +Y T E+V+++ L P + GH P++TL Sbjct: 228 KTCSGFILGQF-KNCTLPHYERSLTLEEVFKDKIFSLNKPTLTHFMSGHDYPKLTL 282 >alr3273 [V] COG1619 Uncharacterized proteins, homologs of microcin C7 resistance protein MccF Length = 359 Score = 88.6 bits (218), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 0/323 (0%), Positives = 0/323 (0%), Gaps = 40/323 (12%) Query: 17 ITYPVIKKGAAIGVTAASAGVGPQSVELFGQAITRMEEK---GYHVLCGDTVFRHDKAKS 73 + + ++ E+ ++ Sbjct: 65 RLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEA---SLDEPVTVWM 121 Query: 74 ASGLVRGKEFNQMMSSDEVDLILPPWGGELLIEMLEHIDFSQVKN--KWIMGFSDISLLL 131 + ++ + + +I + + Sbjct: 122 KL---DTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIF 178 Query: 132 LVITLRTGIATAHGP----SLGDLRGEVIDKT--TAKWETVLSTASGESVIQRSSPTDWK 185 + L + + L S + Sbjct: 179 NTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVM--S 236 Query: 186 TVSGEAVTLQGRLLGGCIDVIRHIIGTPYGDVRHFQENFIHQEPILWYLENCELNTADLR 245 L+ +G + + Sbjct: 237 L--------TSSLIAVREHKAGEPVGYGG--TWVSERDTR-------LGVVAMGYGDGYP 279 Query: 246 RSLVQMKLAGWFDNCSGVMFGRSG--SNTPMENYTAEDVYRELTEELQVPVVYDLDYGHI 303 R+ ++ + + + + + V + Sbjct: 280 RAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERIAEMTK 339 Query: 304 PPQMTLINGAYAEVKVSGGAGTI 326 LI Sbjct: 340 VSAYELITR-- 360 >VCA0439 [V] COG1619 Uncharacterized proteins, homologs of microcin C7 resistance protein MccF Length = 334 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 79/323 (24%), Positives = 142/323 (43%), Gaps = 47/323 (14%) Query: 16 MITYPVIKKGAAIGVTAASAGVGPQSVELFGQAITRMEEKGYHVLCGDTVFRHDKAKSAS 75 M+ + G IG + S+ + F +A ++ +G+ ++ G + D +S S Sbjct: 1 MLYAKALSIGDKIGFFSPSSPATAFAPNRFQRAKAYLKAQGFELVEGSLTGKSDYYRSGS 60 Query: 76 GLVRGKEFNQMMSSDEVDLILPPWGGELLIEMLEHIDFSQVKN--KWIMGFSDISLLLLV 133 R +E NQ++ V I+P GG +L +ID+ ++N K I+G+SD++ LLL Sbjct: 61 IRERAEELNQLIRDPNVRCIMPTIGGNNSNSLLPYIDYEALRNDPKIIIGYSDVTALLLG 120 Query: 134 ITLRTGIATAHGPSLGDLRGE---VIDKTTAKWETVLSTASGESVIQRSS---------- 180 I +TG+ T +GP+L GE ++D+T + +L + + + S Sbjct: 121 IYAQTGLITFYGPALVASFGEYPPLVDETFHSFIDLLCSETNQYQYTMPSSWTDIKHDWE 180 Query: 181 ---------PTDWKTVSGEAVTLQGRLLGGCIDVIRHIIGTPY------GDVRHFQENFI 225 P +W+ + VT GR++GG ++ + I G+ Y GD+ +++ Sbjct: 181 TQHSAKPVYPNEWQFIGKGKVT--GRIIGGNLNTMAGIWGSRYMPEIKVGDILLIEDS-- 236 Query: 226 HQEPILWYLENCELNTADLRRSLVQMKLAGWFDNCSGVMFGRSG-SNTPMENYTAEDVYR 284 L +EN E RS + G F+ S ++ G+ + T DV Sbjct: 237 -----LKGIENVE-------RSFAHLAACGVFERVSAIILGKHELFDNKGTGRTPLDVLI 284 Query: 285 ELTEELQVPVVYDLDYGHIPPQM 307 E+ + VP+ Y D H P + Sbjct: 285 EVLADKNVPIFYGFDSCHTHPML 307 >VCA0337 [V] COG1619 Uncharacterized proteins, homologs of microcin C7 resistance protein MccF Length = 334 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 79/323 (24%), Positives = 142/323 (43%), Gaps = 47/323 (14%) Query: 16 MITYPVIKKGAAIGVTAASAGVGPQSVELFGQAITRMEEKGYHVLCGDTVFRHDKAKSAS 75 M+ + G IG + S+ + F +A ++ +G+ ++ G + D +S S Sbjct: 1 MLYAKALSIGDEIGFFSPSSPATAFAPNRFQRAKAYLKAQGFELVEGSLTGKSDYYRSGS 60 Query: 76 GLVRGKEFNQMMSSDEVDLILPPWGGELLIEMLEHIDFSQVKN--KWIMGFSDISLLLLV 133 R +E NQ++ V I+P GG +L +ID+ ++N K I+G+SD++ LLL Sbjct: 61 IRERAEELNQLIRDPNVRCIMPTIGGNNSNSLLPYIDYEALRNDPKIIIGYSDVTALLLG 120 Query: 134 ITLRTGIATAHGPSLGDLRGE---VIDKTTAKWETVLSTASGESVIQRSS---------- 180 I +TG+ T +GP+L GE ++D+T + +L + + + S Sbjct: 121 IYAQTGLITFYGPALVASFGEYPPLVDETFHSFIDLLCSETNQYQYTMPSSWTDIKHDWE 180 Query: 181 ---------PTDWKTVSGEAVTLQGRLLGGCIDVIRHIIGTPY------GDVRHFQENFI 225 P +W+ + VT GR++GG ++ + I G+ Y GD+ +++ Sbjct: 181 TQHSAKPVYPNEWQFIGKGKVT--GRIIGGNLNTMAGIWGSRYMPEIKVGDILLIEDS-- 236 Query: 226 HQEPILWYLENCELNTADLRRSLVQMKLAGWFDNCSGVMFGRSG-SNTPMENYTAEDVYR 284 L +EN E RS + G F+ S ++ G+ + T DV Sbjct: 237 -----LKGIENVE-------RSFAHLAACGVFERVSAIILGKHELFDNKGTGRTPLDVLI 284 Query: 285 ELTEELQVPVVYDLDYGHIPPQM 307 E+ + VP+ Y D H P + Sbjct: 285 EVLADKNVPIFYGFDSCHTHPML 307 >alr2723 [V] COG1619 Uncharacterized proteins, homologs of microcin C7 resistance protein MccF Length = 359 Score = 65.1 bits (157), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 16/285 (5%), Positives = 41/285 (14%), Gaps = 26/285 (9%) Query: 22 IKKGAAIGVTAASAGVGPQSVELFGQAITRMEEKGYHVLCGDTVFRHDKAKSASGLVRGK 81 + S + +R + Sbjct: 10 RRALRHNLQRLRELAP--ASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLR 67 Query: 82 EFNQMMSSDEVDLILPPWGGELLIEMLEHIDF-SQVKNKWIMGFSDISLLLLVITLRTGI 140 ++ + H ++ + + S + + + Sbjct: 68 AGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWM-KLDTG 126 Query: 141 ATAHGPSLGDLRGEVIDKTTAKWETVLSTASGESVIQRSSPTDWKTVSGEAVTLQGRLLG 200 G + T + V S T + L Sbjct: 127 MHRLGVRPEQAEAFY-HRLT---QCKNVRQPVNIV----SHFARADEPKCGAT--EKQLA 176 Query: 201 GCIDVIRHIIGTPYGDVRHFQENFIHQEPILWYLENCELNTADLRRSLVQMKLAGWFDNC 260 G IL + ++ L Sbjct: 177 IFNTFCEGKPGQR------SIAAS---GGILLWPQSHFDWVRPGIILYGVSPLEDRSTGA 227 Query: 261 SGVMFG---RSGSNTPMENYTAEDVYRELTEELQVPVVYDLDYGH 302 + S + + A + + Sbjct: 228 DFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAM 272 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,914,886 Number of extensions: 989005 Number of successful extensions: 1787 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1774 Number of HSP's successfully gapped: 11 Length of query: 332 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 225 Effective length of database: 26,037,693 Effective search space: 5858480925 Effective search space used: 5858480925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)