BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0057.p (397 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20993 [I] COG1960 Acyl-CoA dehydrogenases 128 1e-29 AF0845 [I] COG1960 Acyl-CoA dehydrogenases 113 4e-25 FN1424_1 [I] COG1960 Acyl-CoA dehydrogenases 110 5e-24 FN0783 [I] COG1960 Acyl-CoA dehydrogenases 105 2e-22 Cgl0988 [I] COG1960 Acyl-CoA dehydrogenases 102 8e-22 >SMb20993 [I] COG1960 Acyl-CoA dehydrogenases Length = 403 Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 118/371 (31%), Positives = 181/371 (48%), Gaps = 25/371 (6%) Query: 34 AKETDREGRYPYTGINALRVAGFTGLSVPVKDGGLGAGYRGDAVLLTAVLMELASWCSST 93 A E D E P+ ++ L +G +S+P + G+ V+L + L+ SS Sbjct: 39 ASERDLERVLPHDELDQLAQSGLLAISIPSEYEGIDVSN----VVLAEITAILSEADSSI 94 Query: 94 SQIFALHNTGVQLVHALGTAEQQQFFFKEAVEGHLFASFGSEANK-----DRTALTSVLE 148 QI H ++ + T EQ+QFFF A+ G F S SE+ D+T +T Sbjct: 95 GQIPQGHFYVLEALRQHATEEQKQFFFGRAMAGDRFGSALSESGDKIIGDDKTRITG--- 151 Query: 149 HTDGGYLLNGKKIFATGSPGAKWAFWRSVSAEASGSQDERYMMPIVQLSADGVTVSDDWD 208 GY +NG+K + TG +A W ++SA + +R + V +A+GV + DDWD Sbjct: 152 -NGAGYRINGRKQYCTG---VLFADWLAISALDAA---DRMTISFVPRAAEGVRIIDDWD 204 Query: 209 GIGQRGTGSGRVEAVNVFVPDGHVAGGPGAYSACSSFFAAQFHVNFAAQFVGMAAGAFRE 268 GQR TGSG NV V G V + ++ + + A +G+A AFRE Sbjct: 205 SFGQRVTGSGTAVLENVHVDLGSVVPHHRGFERPNTVRSVG-QIIHAGVDLGIARAAFRE 263 Query: 269 ARQYILEQARPWQGAG--RAAEDPFTLLRMGDMAVRIDSARQLVLRASRLLGACHEQP-- 324 + + A +G+G RA+ED T R+G++A+R+++A L+ A R + P Sbjct: 264 TLDLVRDAAAA-KGSGMERASEDALTTARVGEVAIRLEAATALIEHAGRKVDTAQVDPTE 322 Query: 325 ELLPEAQTAASRAKVAATDAALEVTGSVFQVMGARAASRKYGIDMYYRNARTLTLHDPVD 384 E EA + + AKV A +AALE ++F + G+ +A +D Y+RNAR L DPV Sbjct: 323 EHAVEASLSVTAAKVLAAEAALEAANALFDLAGSASAKVALNLDRYWRNARAHALQDPVR 382 Query: 385 RQREAVGRYEL 395 + VG Y L Sbjct: 383 WKHHLVGNYHL 393 >AF0845 [I] COG1960 Acyl-CoA dehydrogenases Length = 381 Score = 113 bits (283), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 99/374 (26%), Positives = 160/374 (42%), Gaps = 42/374 (11%) Query: 22 VRRLGAAVIGPSAKETDREGRYPYTGINALRVAGFTGLSVPVKDGGLGAGYRGDAVLLTA 81 VR I P KE D +G YP+ G+ +P K GG G Y G A++ Sbjct: 16 VREFAEKEIMPYGKEYDEKGEYPWEIFRKAAKLGYVAADIPEKYGGAGLDYLGCAIIAE- 74 Query: 82 VLMELASWCSSTSQIFALHNTGVQLVHALGTAEQQQFFFKEAVEGHLFASFG---SEANK 138 E SS + G +V GT EQ++ + + V+G ++ +A Sbjct: 75 ---EFTRADSSIGSAVLTASLGCPMVKYFGTEEQKERYLPKVVKGETPSAIAITEPDAGS 131 Query: 139 DRTALTSVLEHTDGGYLLNGKKIFATGSPGAKWAFWRSVSAEASGSQDERYMMPIVQLSA 198 D A+ + E DGG+ LNG K F T ++W + + +G + Sbjct: 132 DVQAIKTKAERVDGGWKLNGTKTFITNGSVSEWVVILAKTQPDAGYR-----------GI 180 Query: 199 DGVTVSDDWDG--------IGQRGTGSGRVEAVNVFVPDGHVAGGP--GAYSACSSFFAA 248 V WDG +G + + +VFVP+ H+ G G Y F + Sbjct: 181 SAFLVETGWDGYEARRIKKMGLNCHDTAEIYLKDVFVPETHIIGQENFGFYQLMRFFNES 240 Query: 249 QFHVNFAAQFVGMAAGAFREARQYILEQARPWQGAGRA-AEDPFTLLRMGDMAVRIDSAR 307 + V A Q +GMA GA+ A LE A+ + G+ E ++ DM I++A+ Sbjct: 241 RVFVG-AVQ-LGMAVGAYERA----LEYAKQRKAFGKPLIEHQAIQFKLADMWKDIEAAK 294 Query: 308 QLVLRASRLLGACHEQPELLPEAQTAASRAKVAATDAALEVTGSVFQVMGARAASRKYGI 367 L+ +A+ L+ P L +S AK+ A++ A++ T Q+ G S++Y I Sbjct: 295 LLIYKAAWLIDRGKANPAL-------SSAAKLFASETAIKATYEALQIFGGYGYSKEYDI 347 Query: 368 DMYYRNARTLTLHD 381 + YYR+AR T+++ Sbjct: 348 ERYYRDARVGTIYE 361 >FN1424_1 [I] COG1960 Acyl-CoA dehydrogenases Length = 377 Score = 110 bits (274), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 107/387 (27%), Positives = 179/387 (46%), Gaps = 27/387 (6%) Query: 12 TPAQYEVL-LRVRRLGAAVIGPSAKETDREGRYPYTGINALRVAGFTGLSVPVKDGGLGA 70 T ++E L ++VR + P A D+E ++P+ I GF GL P + GG G Sbjct: 5 TTEEHEALRMQVREFVETEVKPIAAILDKENKFPHEAIKKFGQMGFMGLPYPKEYGGAGK 64 Query: 71 GYRGDAVLLTAVLMELASWCSSTSQIFALH-NTGVQLVHALGTAEQQQFFFKEAVEGHLF 129 D + + EL+ T I + H + G + A GT EQ++ + +G Sbjct: 65 ----DILSYAIAVEELSRVDGGTGVILSAHVSLGSYPIFAFGTEEQKKKYLTPLAKGEKL 120 Query: 130 ASFG-SEANKDRTA---LTSVLEHTDGGYLLNGKKIFATGSPGAKWAFWRSVSAEASGSQ 185 +FG +E N A T+ ++ D Y+LNG+KIF T + A+ +V+ G++ Sbjct: 121 GAFGLTEPNAGSDAGGTETTAVKEGD-YYILNGEKIFITNADVAETYVVFAVTTPDIGTK 179 Query: 186 DERYMMPIVQLSADGVTVSDDWDGIGQRGTGSGRVEAVNVFVPDGHVAG--GPGAYSACS 243 IV+ +G T D +D +G R + + ++ NV VP ++ G G G A S Sbjct: 180 GISAF--IVEKGWEGFTFGDHYDKLGIRSSSTCQLLFNNVKVPKENLLGKEGDGFKIAMS 237 Query: 244 SFFAAQFHVNFAAQFVGMAAGAFREARQYILEQARPWQGAGRAAEDPFTLLRMGDMAVRI 303 + + + AAQ +G+A GAF A +Y E R G A + + ++ DMA ++ Sbjct: 238 TLDGGR--IGIAAQALGIAQGAFEHALEYAKE--REQFGKPIAFQQAIS-FKLADMATKL 292 Query: 304 DSARQLVLRASRLLGACHEQPELLPEAQTAASRAKVAATDAALEVTGSVFQVMGARAASR 363 +AR L+ A+ L H +P + A AK A+D ALEV Q+ G + Sbjct: 293 RTARFLIYSAAEL--KEHHEPYGMESAM-----AKQYASDIALEVVNDAVQIFGGSGYLK 345 Query: 364 KYGIDMYYRNARTLTLHDPVDRQREAV 390 ++ YR+A+ T+++ + + V Sbjct: 346 GMEVERAYRDAKITTIYEGTNEIQRVV 372 >FN0783 [I] COG1960 Acyl-CoA dehydrogenases Length = 381 Score = 105 bits (261), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 96/379 (25%), Positives = 175/379 (46%), Gaps = 27/379 (7%) Query: 12 TPAQYEVLLR-VRRLGAAVIGPSAKETDREGRYPYTGINALRVAGFTGLSVPVKDGGLGA 70 P +E+ + +R + P A E D R+P + + G G+ +P + GG G Sbjct: 5 VPKTHELFRQMIREFVEKEVKPIAAEVDENERFPVETVEKMAKIGIMGIPIPKQYGGAG- 63 Query: 71 GYRGDAVLLTAVLMELASWCSSTSQIFALHNT-GVQLVHALGTAEQQQFFFKEAVEGHLF 129 GD ++ + EL+ C++T I + H + G + G +Q+Q + + G Sbjct: 64 ---GDNLMYAMAVEELSKACATTGVIVSAHTSLGTWPILKFGNEKQKQKYLPKMASGEWI 120 Query: 130 ASFG-SEANKDRTA----LTSVLEHTDGGYLLNGKKIFATGSPGAKWAFWRSVSAEASGS 184 +FG +E N A +V + G ++LNG KIF T A +A V A S Sbjct: 121 GAFGLTEPNAGTDAAGQQTMAVQDPETGEWILNGAKIFITN---AGYAHVYVVFAMTDKS 177 Query: 185 QDERYMMP-IVQLSADGVTVSDDWDGIGQRGTGSGRVEAVNVFVPDGHVAGGPGAYSACS 243 + + + IV+ + G ++ +G RG+ + + N +P ++ G G + Sbjct: 178 KGLKGISAFIVEANTPGFSIGKKEMKLGIRGSATCELIFENCRIPKENLLGDKGKGFKIA 237 Query: 244 SFFAAQFHVNFAAQFVGMAAGAFREARQYILEQARPWQGAGRA-AEDPFTLLRMGDMAVR 302 + A+Q +G+AAGA EA Y A+ + GR+ A+ T ++ ++ V+ Sbjct: 238 MMTLDGGRIGIASQALGIAAGALDEAINY----AKERKQFGRSLAQFQNTQFQIANLDVK 293 Query: 303 IDSARQLVLRASRLLGACHEQPELLPEAQTAASRAKVAATDAALEVTGSVFQVMGARAAS 362 +++AR LV +A+ + LP + AA RAK+ A + A+EVT Q+ G + Sbjct: 294 VEAARLLVYKAA------WRESNNLPYSLDAA-RAKLFAAETAMEVTTKAVQIFGGYGYT 346 Query: 363 RKYGIDMYYRNARTLTLHD 381 R+Y ++ R+A+ +++ Sbjct: 347 REYPVERMMRDAKITEIYE 365 >Cgl0988 [I] COG1960 Acyl-CoA dehydrogenases Length = 399 Score = 102 bits (255), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 104/370 (28%), Positives = 159/370 (42%), Gaps = 17/370 (4%) Query: 34 AKETDREGRYPYTGINALRVAGFTGLSVPVKDGGLGAGYRGDAVLLTAVLMELASWCSST 93 A E +++ P I L GFTGL VP + GG GA + +L+E+A S+ Sbjct: 20 AVEREQQRALPVEEIKELVELGFTGLRVPEELGGAGASLES----VVELLIEIAGADSNI 75 Query: 94 SQIFALHNTGVQLVHALGTAEQQQFFFKEAVEGHLFASFGSEANK---DRTALTSVLEHT 150 +Q H V+L+ +E + + +E G L + SE D +E Sbjct: 76 AQALRGHFAFVELLLEAPESEFRTHWLREVATGRLVGNAESEKRGVYGDPQTFIDEVETE 135 Query: 151 DGG-YLLNGKKIFATGSPGAKWAFWRSVSAEASGSQDERYMMPIVQLSADGVTVSDDWDG 209 +G ++LNG K + TG+ A + W + Q+ +P V L A GV V DDW G Sbjct: 136 NGPIFVLNGTKFYTTGTYFADYT-WTTALLRNLNGQETLVSLP-VDLHAPGVDVVDDWSG 193 Query: 210 IGQRGTGSGRVEAVNVFVPDGHVAGGPGAYSACSSFFAAQFHVNFAAQFVGMAAGAFREA 269 GQ+ T SG ++ V + A + ++ ++ VG AA A E Sbjct: 194 FGQKLTASGTTTFKDLEVDPRWIIPRTDAPTLVWTYL----QLSLLTVLVGSAAAAVDEV 249 Query: 270 RQYILEQAR-PWQGAGRAAEDPFTLLRMGDMAVRIDSARQLVLRASRLL--GACHEQPEL 326 R W DP + +GD R+ R +L A+R + A PE Sbjct: 250 VARAQSSTRNAWNPGVERRSDPAATIAIGDARSRVTVIRGALLDATRHVSNAATIVTPEA 309 Query: 327 LPEAQTAASRAKVAATDAALEVTGSVFQVMGARAASRKYGIDMYYRNARTLTLHDPVDRQ 386 EA + + AL VT +VF +GA A ++ ID ++RN RT++ ++PV Sbjct: 310 FNEADAIVAALWPIVSGQALVVTSNVFDAVGASAVLGEHSIDRHWRNVRTVSSNNPVFLA 369 Query: 387 REAVGRYELG 396 + AVG Y L Sbjct: 370 KNAVGEYALN 379 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.133 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,519,388 Number of extensions: 1208576 Number of successful extensions: 2308 Number of sequences better than 1.0e-05: 82 Number of HSP's gapped: 2205 Number of HSP's successfully gapped: 82 Length of query: 397 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 288 Effective length of database: 25,807,025 Effective search space: 7432423200 Effective search space used: 7432423200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)