BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0056.p (310 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 292 5e-79 PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 290 1e-78 Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 247 1e-65 BS_ytnJ [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 177 2e-44 Cgl0697 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 170 2e-42 >PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 292 bits (747), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 0/304 (0%), Positives = 0/304 (0%), Gaps = 3/304 (0%) Query: 3 LPHDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGY 62 P D+ D E R K + + Sbjct: 152 APAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLL 211 Query: 63 HSSEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRD 122 + S ++ G +L R + R Sbjct: 212 NRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQ 271 Query: 123 PAAVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFEN 182 A + A S ++ + + S Sbjct: 272 KAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVL 331 Query: 183 IHTEMGQTHTDRFTKHSKTKK-TVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETD 241 Sbjct: 332 - 390 Query: 242 VDGFNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGT 301 Sbjct: 391 - 449 Query: 302 VFRN 305 Sbjct: 450 453 >PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 290 bits (743), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 0/304 (0%), Positives = 0/304 (0%), Gaps = 2/304 (0%) Query: 3 LPHDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGY 62 + + + Sbjct: 153 PAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLN 212 Query: 63 HSSEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRD 122 S E L A R + E + Sbjct: 213 RSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQK 272 Query: 123 PAAVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFEN 182 A V + + + + + T I+ L+ Sbjct: 273 AVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLA 332 Query: 183 IHTEMGQTHTDRFTKHSKTKKTVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETDV 242 Sbjct: 333 -- 390 Query: 243 DGFNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGTV 302 Sbjct: 391 450 Query: 303 FRNP 306 Sbjct: 451 454 >Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 247 bits (631), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 0/303 (0%), Positives = 0/303 (0%), Gaps = 3/303 (0%) Query: 3 LPHDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGY 62 L Y A + + + + ++ + + + + F++ Sbjct: 161 LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLA 220 Query: 63 HSSEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRD 122 S+ G + + R + ++ Y Sbjct: 221 EVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDI 280 Query: 123 PAAVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFEN 182 + + ++ + T ++ + + ++ + Sbjct: 281 ARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQV 340 Query: 183 IHTEMGQTHTDRFTKHS-KTKKTVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETD 241 Sbjct: 341 - 399 Query: 242 VDGFNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGT 301 Sbjct: 400 - 458 Query: 302 VFR 304 Sbjct: 459 461 >BS_ytnJ [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 177 bits (448), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 0/302 (0%), Positives = 0/302 (0%), Gaps = 15/302 (4%) Query: 3 LP-HDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTG 61 DSA L + A KI V + H L Sbjct: 142 GKLGYSWTDSAPAVSLLDTLDAL-AEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPR 200 Query: 62 YHSSEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGR 121 + + F+ G ++ + + L T Sbjct: 201 IVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFS 260 Query: 122 DPAAVKVFTGLSVVVGRTKEEAERKHTEY---LSYQSAEATLSSYANVTGIDLTKLD-PD 177 V L++ + ++S + Sbjct: 261 RFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIE 320 Query: 178 SYFENIHTEMGQTHTDRFTKHSKTKK-TVREVTEDFLKKGFRGLTVIGTPEEIADQIQYW 236 S + ++ ++ Sbjct: 321 SLHLELSEDVLAEIEAVHQVYTYPAP--- 377 Query: 237 VEETDVDGFNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPE 296 Sbjct: 378 ----- 432 Query: 297 HH 298 Sbjct: 433 434 >Cgl0697 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 170 bits (431), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 0/291 (0%), Positives = 0/291 (0%), Gaps = 12/291 (4%) Query: 5 HDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGYHS 64 DSA L + A KI + + Sbjct: 145 GYSWTDSAPAVSLLDTLDAL-AEYQRAGKIRYIGV-SNETAFGVMRYLHLADKHDLPRIV 202 Query: 65 SEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRDPA 124 + + + T + AR Sbjct: 203 TIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSR- 261 Query: 125 AVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYF---- 180 ++G + Q A A + V L D Sbjct: 262 -FTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIE 320 Query: 181 --ENIHTEMGQTHTDRFTKHSKTKKTVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVE 238 +E + + Sbjct: 321 SLHLELSEDVLAEIEAVHQVYTYP-- 378 Query: 239 ETDVDGFNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGK 289 Sbjct: 379 429 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,513,787 Number of extensions: 1001783 Number of successful extensions: 3310 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 3304 Number of HSP's successfully gapped: 15 Length of query: 310 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 204 Effective length of database: 26,153,027 Effective search space: 5335217508 Effective search space used: 5335217508 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)