BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0055.p (152 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 139 2e-33 PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 130 6e-31 Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 118 3e-27 SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene te... 80 1e-15 mll5137 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 75 3e-14 >PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 139 bits (349), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 10/134 (7%), Positives = 34/134 (25%), Gaps = 3/134 (2%) Query: 2 KQIHLGVFEVNSINHLTQGIWAHPEQNRVHYRKIGYWTRIAKILERGKFDFMFFAD---T 58 +I EV+++ T + H + + +++ + + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 59 YGYPKERTELAFREATGLPGNDPMLLIPALSMVTEHLAFTMTTSTSYEAPFANARRFSSL 118 LA + + P+ + + ++ + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 119 DHVTDGRIGWDVGT 132 D++ ++ W Sbjct: 124 DYLDLYQVHWPQRP 137 >PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 130 bits (328), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 5/134 (3%), Positives = 16/134 (11%), Gaps = 3/134 (2%) Query: 2 KQIHLGVFEVNSINHLTQGIWAHPEQNRVHYRKIGYWTRIAKILERGKFDFMFFADT--- 58 S L + + ++ Y L + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 59 YGYPKERTELAFREATGLPGNDPMLLIPALSMVTEHLAFTMTTSTSYEAPFANARRFSSL 118 + + + + + + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 119 DHVTDGRIGWDVGT 132 D Sbjct: 123 TDYLDLYQVHWPQR 136 >Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 118 bits (295), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 5/134 (3%), Positives = 19/134 (14%), Gaps = 3/134 (2%) Query: 2 KQIHLGVFEVNSINHLTQGIWAHPEQNRVHYRKIGYWTRIAKILERGKFDFMFFAD---T 58 + LG+ + ++ V + Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 59 YGYPKERTELAFREATGLPGNDPMLLIPALSMVTEHLAFTMTTSTSYEAPFANARRFSSL 118 + E + + + S + + + Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV 131 Query: 119 DHVTDGRIGWDVGT 132 + Sbjct: 132 HWPQRPTNCFGKLG 145 >SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 79.7 bits (195), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 9/133 (6%), Positives = 19/133 (14%), Gaps = 9/133 (6%) Query: 2 KQIHLGVFEVNSINHLTQGIWAHPEQNRVHYRKIGYWTRIAKILERGKFDFMFFADTYGY 61 S L EQN +A+ + M+ Sbjct: 3 YHRIPHSSLEVSTLGLG--TMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRP- 59 Query: 62 PKERTELAFREA--TGLPGNDPMLLIPALSMVTEHLAFTMTTSTSYEAPFANARRFSSLD 119 E ++ + + +L Sbjct: 60 ----ETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALH 115 Query: 120 HVTDGRIGWDVGT 132 + Sbjct: 116 DSLKRLQTDYLDL 128 >mll5137 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 75.5 bits (184), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 15/130 (11%), Positives = 22/130 (16%), Gaps = 3/130 (2%) Query: 3 QIHLGVFEVNSINHLTQGIWAHPEQNRVHYRKIGYWTRIAKILERGKFDFMFFADTYGYP 62 S L EQN +A+ + M+ Sbjct: 4 HRIPHSSLEVSTLGLG--TMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 63 KERTELAFREATGLPGNDPMLLIPALSMVTEHLAFTMTTSTSYEAPFANARRFSSLDHVT 122 + TE LI A + N R Sbjct: 62 QGLTETYVGNWLAK-HGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 123 DGRIGWDVGT 132 D+ Sbjct: 121 LQTDYLDLYQ 130 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.137 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 10,899,205 Number of extensions: 439715 Number of successful extensions: 528 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 519 Number of HSP's successfully gapped: 10 Length of query: 152 Length of database: 38,378,431 Length adjustment: 97 Effective length of query: 55 Effective length of database: 27,191,033 Effective search space: 1495506815 Effective search space used: 1495506815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 111 (47.4 bits)