BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0048.p (410 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR0339 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 394 e-109 DR1711 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 379 e-105 alr4934 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 319 6e-87 slr1653 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 309 6e-84 BH1613 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 306 6e-83 >DR0339 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 392 Score = 394 bits (1012), Expect = e-109, Method: Compositional matrix adjust. Identities = 195/374 (52%), Positives = 251/374 (67%), Gaps = 1/374 (0%) Query: 5 LAIVENWPPELQEQLVAWRRHLHLNPELSFEEHHTAAYVEKNLREIGGLEISRPTPTSVV 64 + E+ L+ QL AWRRHLH++PELSF+E TA Y+ L ++ GLE+S+ TPTSV+ Sbjct: 1 MTATEDRVSALRPQLEAWRRHLHMHPELSFQEFETARYIVGELNKLPGLEVSQLTPTSVL 60 Query: 65 ARLIGGKPGKTLALRADMDALPIHEENEFEFASAVPGVMHACGHDGHTSILLGVVSLLAG 124 A L GG+PG+T+ LRAD+DALPI EEN FEF S PGVMHACGHDGHT+ILLG LLA Sbjct: 61 AVLRGGQPGRTVLLRADIDALPIQEENSFEFVSQNPGVMHACGHDGHTAILLGTAQLLAE 120 Query: 125 QRGQLHGEIRFIFQHAEEXXXXXXXXXXXXX-XXXXXDYIVGEHLASQLPLGQIGVAYGE 183 + GEIR IFQHAEE D + G HL SQLP GQ+ V G Sbjct: 121 DAANVPGEIRMIFQHAEENGPGGAEELVMNTPLMDGVDVVTGLHLDSQLPAGQVVVKAGA 180 Query: 184 MMASPDNFTIVVKGYGGHGGFPHKSVDPIAIGAQIVSNLQHIASRNTNPLDRLVVSITQF 243 MA+PD + ++G GGHG P ++VDPIAIGAQ+V+NLQH+ SRN + D LVVSIT F Sbjct: 181 FMAAPDMIELTIRGKGGHGAHPDETVDPIAIGAQVVTNLQHVVSRNVSAQDALVVSITYF 240 Query: 244 TSGSSHNVIPDTATLKGTVRSFDPQVRKDAQQQLRRIVEGITNAHGAEYELDYLLGYDPV 303 SGS+HNVIPDTA L GTVR+FDP +R+ A Q + R+++G+ AHGA Y+L Y LGY PV Sbjct: 241 QSGSTHNVIPDTARLMGTVRTFDPDLRRKAPQLIERVIKGVCEAHGATYDLKYELGYRPV 300 Query: 304 VNDHTLTRLAEEAAVELVGRDNVVQTPPGMSGEDFSAYQQRVPGTFINIGAGNPDKGILY 363 +N + + A+ VG ++ + M GEDFSAY ++ PG + N+G+G+ + + Sbjct: 301 INTEWVAEELRQIALNTVGAEHFAEAKATMGGEDFSAYLEKAPGAYFNVGSGSDEHDSRW 360 Query: 364 PHHHPRFTFDEAAL 377 PHHHPRFT DE +L Sbjct: 361 PHHHPRFTIDELSL 374 >DR1711 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 388 Score = 379 bits (972), Expect = e-105, Method: Compositional matrix adjust. Identities = 189/363 (52%), Positives = 237/363 (65%) Query: 15 LQEQLVAWRRHLHLNPELSFEEHHTAAYVEKNLREIGGLEISRPTPTSVVARLIGGKPGK 74 L+EQ+VAWRRHLH +PEL F+EH TA YVE+ LR++ GL +SRPT TSV+A L GGKPG+ Sbjct: 9 LREQVVAWRRHLHQHPELGFQEHQTARYVEEQLRDMPGLHLSRPTETSVLAVLKGGKPGR 68 Query: 75 TLALRADMDALPIHEENEFEFASAVPGVMHACGHDGHTSILLGVVSLLAGQRGQLHGEIR 134 T+ LRADMDALPI EE + EF S PGVMHACGHDGHT++LLG L+ Q LHGE+R Sbjct: 69 TVLLRADMDALPIEEEADVEFKSQTPGVMHACGHDGHTAMLLGAAKQLSAQAADLHGEVR 128 Query: 135 FIFQHAEEXXXXXXXXXXXXXXXXXXDYIVGEHLASQLPLGQIGVAYGEMMASPDNFTIV 194 FIFQHAEE D VG HL + +P+G + + G +MA+PD F I Sbjct: 129 FIFQHAEEVFPGGAEQLVDAGVMDGVDLAVGTHLMTSVPVGTVVLRDGALMAAPDAFDIT 188 Query: 195 VKGYGGHGGFPHKSVDPIAIGAQIVSNLQHIASRNTNPLDRLVVSITQFTSGSSHNVIPD 254 ++G GGHG PH++VDP+ I AQ+V Q SR +P+D VVS+TQ GS+HNVIPD Sbjct: 189 IQGKGGHGAMPHQTVDPVVIAAQVVMAFQTAVSRLRDPIDPGVVSVTQIHGGSAHNVIPD 248 Query: 255 TATLKGTVRSFDPQVRKDAQQQLRRIVEGITNAHGAEYELDYLLGYDPVVNDHTLTRLAE 314 T TL GTVR+F ++R +L ++ GI A+GA Y Y GY V ND T Sbjct: 249 TVTLGGTVRTFSDELRAQMPGRLETLLRGICEAYGATYTFTYHGGYRSVNNDPATTERLR 308 Query: 315 EAAVELVGRDNVVQTPPGMSGEDFSAYQQRVPGTFINIGAGNPDKGILYPHHHPRFTFDE 374 + E++ V P M GEDFSAY R PGTF+ IGAGN +KG+ PHHHP+F DE Sbjct: 309 KVVREVLPEVTVSDGVPLMGGEDFSAYLTRAPGTFVLIGAGNAEKGMTAPHHHPKFMIDE 368 Query: 375 AAL 377 +AL Sbjct: 369 SAL 371 >alr4934 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 359 Score = 319 bits (817), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 0/373 (0%), Positives = 0/373 (0%), Gaps = 15/373 (4%) Query: 15 LQEQLVAWRRHLHLNPELSFEEHHTAAYVEKNLREIGGLEISRPTPTSVVARLIGGKP-G 73 L E E R + ++ + Sbjct: 23 LAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFF 82 Query: 74 KTLALRADMDALPIHEENEFEFASAVPGVMHACGHDGHTSILLGVVSLLAGQRGQLHGEI 133 L + E +A+ + G+ L Sbjct: 83 DARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYH 142 Query: 134 RFIFQHAEEXXXXXXXXXXXXXXXX-XXDYIVGEHLASQLPLGQIGVAYGEMMASPDNFT 192 R + + Sbjct: 143 RLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQ 202 Query: 193 IVVKGYGGHGGFPHKSVDPIAIGAQIVSNLQHIASRNTNPLDRLVVSITQFTSGSSHNVI 252 S ++ ++ R + G + Sbjct: 203 SHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSE 262 Query: 253 PDTATLKGTVRSFDPQVRKDAQQQLRRIVEGITNAHGAEYELDYLLGYDPVVNDHTLTRL 312 DT + D R + G + P D + Sbjct: 263 RDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPV 322 Query: 313 AEEAAVELVGRDNVVQTPPG-------MSGEDFSAYQQRVPGTFINIGAGNPDKGILYPH 365 V+ Sbjct: 323 ILWGEGLP------VERIAEMTKVSAY 376 Query: 366 HHPRFTFDEAALA 378 Sbjct: 377 389 >slr1653 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 416 Score = 309 bits (791), Expect = 6e-84, Method: Compositional matrix adjust. Identities = 162/365 (44%), Positives = 217/365 (59%), Gaps = 2/365 (0%) Query: 15 LQEQLVAWRRHLHLNPELSFEEHHTAAYVEKNLREIGGLEISRPTPTSVVARLIGGKPGK 74 L QL+ WRR H PEL F+E TAA++ + L ++ T ++A + GKPG Sbjct: 35 LHGQLIQWRRQFHQYPELGFQEQLTAAHIAETLTKLEIPHTPGIAKTGIMATVDSGKPGP 94 Query: 75 TLALRADMDALPIHEENEFEFASAVPGVMHACGHDGHTSILLGVVSLLAGQRGQLHGEIR 134 LA+RADMDALP+ EENE ++ S PG MHACGHDGHT+I LG LA R G+++ Sbjct: 95 VLAIRADMDALPVTEENEVDYRSLHPGKMHACGHDGHTAIALGTAQYLAAHRDSFRGQVK 154 Query: 135 FIFQHAEEXXXXXXXXXXX-XXXXXXXDYIVGEHLASQLPLGQIGVAYGEMMASPDNFTI 193 F FQ AEE D IVG HL + LP+G +G+ G +MA+ ++F Sbjct: 155 FFFQPAEEGPGGAKPMIEAGVLENPAVDAIVGLHLWNDLPVGTVGIKPGPVMAAVEHFEC 214 Query: 194 VVKGYGGHGGFPHKSVDPIAIGAQIVSNLQHIASRNTNPLDRLVVSITQFTSGSSHNVIP 253 + G GGHG PH++VD + I AQIV LQ I +RN NPL VV++ Q SG++ NVIP Sbjct: 215 QLFGQGGHGAMPHQTVDTLVISAQIVMALQGIVARNLNPLQSAVVTVGQLQSGTAFNVIP 274 Query: 254 DTATLKGTVRSFDPQVRKDAQQQLRRIVEGITNAHGAEYELDYLLGYDPVVNDHTLTRLA 313 D+A +GTVR FDP Q++ I++GI +HGA Y+ Y Y PVVND L L Sbjct: 275 DSAYFRGTVRYFDPSFAGYFAQRIEEIIKGICQSHGANYQFTYENIYPPVVNDRRLADLV 334 Query: 314 EEAAVELVGRDNVVQTP-PGMSGEDFSAYQQRVPGTFINIGAGNPDKGILYPHHHPRFTF 372 AA +++ D+ +Q ++GED S + Q VPG + +G+ N D G+ YPHHHPRF F Sbjct: 335 RSAAADVLLTDDHLQPDYQTLAGEDMSFFLQAVPGCYFFLGSANGDLGLAYPHHHPRFNF 394 Query: 373 DEAAL 377 DEA L Sbjct: 395 DEAVL 399 >BH1613 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 404 Score = 306 bits (783), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 160/362 (44%), Positives = 210/362 (58%) Query: 17 EQLVAWRRHLHLNPELSFEEHHTAAYVEKNLREIGGLEISRPTPTSVVARLIGGKPGKTL 76 ++LV RR LH +PELSFEE T A++ L ++G VV + GGKPG T+ Sbjct: 14 DELVDIRRTLHKHPELSFEEVKTPAFIADYLEKLGVNVRRGVGGRGVVGTIKGGKPGPTV 73 Query: 77 ALRADMDALPIHEENEFEFASAVPGVMHACGHDGHTSILLGVVSLLAGQRGQLHGEIRFI 136 ALRAD DALPI EE + S VPGVMHACGHDGHT+ LL + +L R QL G + I Sbjct: 74 ALRADFDALPIQEETGLPYQSTVPGVMHACGHDGHTATLLIIAKILMEHRQQLEGSVVLI 133 Query: 137 FQHAEEXXXXXXXXXXXXXXXXXXDYIVGEHLASQLPLGQIGVAYGEMMASPDNFTIVVK 196 Q AEE D I G HL + +P G+IG G MA+ D F + + Sbjct: 134 HQFAEELAPGGAIAMIEDGCLDGVDVIYGTHLWATMPAGEIGYRSGAFMAAADKFAVTIY 193 Query: 197 GYGGHGGFPHKSVDPIAIGAQIVSNLQHIASRNTNPLDRLVVSITQFTSGSSHNVIPDTA 256 G GGHG PH++VD + +GA +V LQ I SRN +PL+ VV++ F +G NVI D A Sbjct: 194 GKGGHGAAPHETVDAVTVGASVVQQLQQIVSRNVDPLESAVVTVASFQAGGPFNVIADKA 253 Query: 257 TLKGTVRSFDPQVRKDAQQQLRRIVEGITNAHGAEYELDYLLGYDPVVNDHTLTRLAEEA 316 L GTVR+F ++ +++ R ++G+ +A GA Y Y GY V+N T + A Sbjct: 254 NLVGTVRTFKEDIQDMIIERMERTIKGVCDAAGATYAFAYDKGYPAVINHPKETDDLKNA 313 Query: 317 AVELVGRDNVVQTPPGMSGEDFSAYQQRVPGTFINIGAGNPDKGILYPHHHPRFTFDEAA 376 AV +VG ++V + P M GEDF+ Y VPG F GAGN D GI+YPHHHP+F DE + Sbjct: 314 AVAVVGEEHVAEMAPVMGGEDFAYYLHHVPGAFFFTGAGNEDAGIIYPHHHPKFMIDERS 373 Query: 377 LA 378 LA Sbjct: 374 LA 375 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,245,198 Number of extensions: 1339004 Number of successful extensions: 3151 Number of sequences better than 1.0e-05: 68 Number of HSP's gapped: 2972 Number of HSP's successfully gapped: 68 Length of query: 410 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 301 Effective length of database: 25,807,025 Effective search space: 7767914525 Effective search space used: 7767914525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)