BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0043.p (293 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 258 1e-68 PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 226 4e-59 Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 207 2e-53 mll2278 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 148 8e-36 SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene te... 141 1e-33 >PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 258 bits (658), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 0/290 (0%), Positives = 0/290 (0%), Gaps = 2/290 (0%) Query: 1 MEVSYRLLEGSWQDDAVSADKEHRYYADADKVHIVKHQGKYYEMTASHACEPSIQRTPVL 60 + + + + + + + E + Sbjct: 166 EYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQY 225 Query: 61 FQAGSSERGRAFAAKHAEGVFLKAPTVEALRAQVQDIRSRAAAYGRLPEQIKIFTGLSAV 120 A A + + + Sbjct: 226 EGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHG 285 Query: 121 VGQTAQEALDKLESYRRYASREAALLTYENSTGIDLTAMDPDAPFIGVQTEQGRTHTERY 180 + ++ A + T I+ ++ + + E Sbjct: 286 LDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSED-VLAEIEAVHQVYTYP 344 Query: 181 TKHSGSVQTVREVMDNFTGKEFRGITVAGTAGEIADQMQYWVEESGIDGFNLERYTLPGN 240 Sbjct: 345 - 403 Query: 241 HREFVDYVVPELQARGMFRRHYEETTLRERLFGQGKQRLPDTHPGAAFRQ 290 Sbjct: 404 453 >PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 226 bits (576), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 0/292 (0%), Positives = 0/292 (0%), Gaps = 3/292 (1%) Query: 1 MEVSYRLLEGSWQDDAVSADKEHRYYADADKVHIVKHQGKYYEMTASHACEPSIQRTPVL 60 E +A RY ADK + + + + Sbjct: 165 AEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQ 224 Query: 61 FQAGSSERGRAFAAKHAEGVFLKAPTVEALRAQVQDIRSRAAAYGRLPEQIKIFTGLSAV 120 ++ G +L R + +R + Sbjct: 225 YEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRH 284 Query: 121 VGQTAQEALDKLESYRRYASREAALLTYENSTGIDLTAMDPDAPFIGVQTEQGRTHTERY 180 AQ AL + AS T + + + + + E Sbjct: 285 GLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYTYP 344 Query: 181 TKHSGS-VQTVREVMDNFTGKEFRGITVAGTAGEIADQMQYWVEESGIDGFNLERYTLPG 239 Sbjct: 345 - 403 Query: 240 NHREFVDYVVPELQARGMFRRHYEETTLRERLFGQGKQRLPDTHPGAAFRQV 291 Sbjct: 404 - 454 >Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 207 bits (526), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 0/290 (0%), Positives = 0/290 (0%), Gaps = 3/290 (1%) Query: 1 MEVSYRLLEGSWQDDAVSADKEHRYYADADKVHIVKHQGKYYEMTASHACEPSIQRTPVL 60 + + +H + + +E+ + + Sbjct: 174 RYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAY 233 Query: 61 FQAGSSERGRAFAAKHAEGVFLKAPTVEALRAQVQDIRSRAAAYGRLPEQIKIFTGLSAV 120 G + R + + AAY + + + A+ Sbjct: 234 SCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMAL 293 Query: 121 VGQTAQEALDKLESYRRYASREAALLTYENSTGIDLTAMDPDAPFIGVQTEQGRTHTERY 180 Sbjct: 294 353 Query: 181 TKHSG-SVQTVREVMDNFTGKEFRGITVAGTAGEIADQMQYWVEESGIDGFNLERYTLPG 239 Sbjct: 354 - 412 Query: 240 NHREFVDYVVPELQARGMFRRHYEETTLRERLFGQGKQRLPDTHPGAAFR 289 Sbjct: 413 - 461 >mll2278 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 148 bits (374), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 0/270 (0%), Positives = 0/270 (0%), Gaps = 5/270 (1%) Query: 6 RLLEGSWQDDAVSADKEHRYYADADKVHIVKHQGKYYEMTASHACEPSIQRTPVLFQAGS 65 L + + A ++ + S+ Sbjct: 162 DALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAE 221 Query: 66 SERGRAFAAKHAEGVFLKAPTVEALRAQVQDIRSRAAAYGRLPEQIKIFTGLSAVVGQTA 125 + + T + L + A A Sbjct: 222 VSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIA 281 Query: 126 QEALDKLESYRRYASREAALLTYENSTGIDLTAMDPDAPFIGVQTEQ----GRTHTERYT 181 + R + + + + + ++ + + Sbjct: 282 RRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVY 341 Query: 182 KHSGSVQTVREVMDNFTGKEFRGITVAGTAGEIADQMQYWVEESGIDGFNLERYTLPGNH 241 Sbjct: 342 - 400 Query: 242 REFVDYVVPELQARGMFRRHYEETTLRERL 271 Sbjct: 401 430 >SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 141 bits (356), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 0/275 (0%), Positives = 0/275 (0%), Gaps = 11/275 (4%) Query: 4 SYRLLEGSWQDDAVSADKEHRYYADADKVHIVKHQGKYYEMTASHACEPSIQRTPVLFQA 63 +Q + + K+ P Sbjct: 159 DTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG 218 Query: 64 GSSERGRAFAAKHAEGVFLKAPTVEALRAQVQDIRSRAAAYGRLPEQIKIFTGLSAVVGQ 123 + A + +R + R T + Sbjct: 219 LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYV 278 Query: 124 TAQEALDKLESYRRYASREAALLTYENSTG--IDLTAMDPDAPF-IGVQTEQGRTHTERY 180 + A G + + + + Sbjct: 279 DIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVH 338 Query: 181 TK----HSGSVQTVREVMDNFTGKEFRGITVAGTAGEIADQMQYWVEESGIDGFNLERYT 236 Sbjct: 339 QVYTYP---- 394 Query: 237 LPGNHREFVDYVVPELQARGMFRRHYEETTLRERL 271 Sbjct: 395 429 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,267,635 Number of extensions: 925300 Number of successful extensions: 2868 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 2860 Number of HSP's successfully gapped: 11 Length of query: 293 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 187 Effective length of database: 26,153,027 Effective search space: 4890616049 Effective search space used: 4890616049 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)