BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0042.p (488 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superf... 608 e-174 lin0519 [GEPR] COG0477 Permeases of the major facilitator superf... 521 e-148 lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 268 2e-71 SPy0570 [GEPR] COG0477 Permeases of the major facilitator superf... 167 3e-41 HI0897 [GEPR] COG0477 Permeases of the major facilitator superfa... 134 3e-31 >BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 479 Score = 608 bits (1567), Expect = e-174, Method: Compositional matrix adjust. Identities = 305/470 (64%), Positives = 358/470 (76%), Gaps = 1/470 (0%) Query: 13 DRKFKTMPILISLLLAGFIGMFSETALNVALSDLMKVLDITPATAQWLTTAYLLTLGILV 72 +++K MPI+ISLLLAGFIGMFSETALN+AL+DLMK L+IT AT QWLTT YLL LGILV Sbjct: 10 SQQYKVMPIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLLVLGILV 69 Query: 73 PISGLLLQWFSTRQLFIAALSFSILGTFLAAMSPNFEFLLIARVVQAMGTALLLPLMFNT 132 P+SGLLLQWF+TRQLF +L FSILGTF+AA++P+F FLL AR+VQA+GT LLLPLMFNT Sbjct: 70 PVSGLLLQWFTTRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNT 129 Query: 133 ILVIIPAEKRGAAMGLIGLVIMVAPAIGPTIAGLLIESLSWHWIFWLSLPFLVIALISGI 192 ILVI P KRGAAMG IGLVIM APAIGPT +GL++E L+WHWIFW+SLPFLV+AL+ GI Sbjct: 130 ILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVLALVFGI 189 Query: 193 FFMQNVTEVTKPKIDXXXXXXXXXXXXXXXXXXXXXXEAEG-WGSPKXXXXXXXXXXXXX 251 +MQNV+E TKPKID E G W SP Sbjct: 190 AYMQNVSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVSLIVGVVGLI 249 Query: 252 XXXXRQMTMKQPMINLRAFKFPMFVVGVLMVFICMMVILSSMLILPMYLQQGQGYSAFKA 311 RQ+TMKQPM+NLRAFK+PMF++GV+MVFICMMVILSSML+LPMYLQ G +AF + Sbjct: 250 LFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFAS 309 Query: 312 XXXXXXXXXXXXXMSPIMGRLFDKYGPKWLVIPGLIIVAVSLWFFSSITATSTVVFVIVL 371 MSP+ GRLFDKYGPKWLVIPG +IV V LWFFS++T TST V +I+L Sbjct: 310 GLVLLPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIIL 369 Query: 372 HSALMVGVSMIFMPAQTNGINQLPMELYPDGTAIMNTLQQVAGAIGTALAVSIMTAGSKS 431 H+ LM+G+SMI MPAQTNG+NQLP E YPDGTAIMNTLQQ+AGAIGTA+AVSIM AG Sbjct: 370 HTCLMIGISMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAVSIMAAGQHD 429 Query: 432 YMESVSNPSDPANLLPAFTQGVQNAFVFGMAMAIIGLVVAFFLKRVIVQH 481 YM +V NP+DPA + A T GVQ+AFVF M +AIIGL+ AFF+KRV V H Sbjct: 430 YMSTVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVKVDH 479 >lin0519 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 471 Score = 521 bits (1343), Expect = e-148, Method: Compositional matrix adjust. Identities = 260/469 (55%), Positives = 343/469 (73%), Gaps = 2/469 (0%) Query: 8 KKQTEDRKFKTMPILISLLLAGFIGMFSETALNVALSDLMKVLDITPATAQWLTTAYLLT 67 ++ T +K + +PI+ S L+AGFIG+FSETALN+ALSDL++V DI+ AT QWLTT YLLT Sbjct: 3 QEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLT 62 Query: 68 LGILVPISGLLLQWFSTRQLFIAALSFSILGTFLAAMSPNFEFLLIARVVQAMGTALLLP 127 LGILVPISGLLLQWF+TR LF A+SFSI GT +AA+SP F L+I RVVQA+GTALLLP Sbjct: 63 LGILVPISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLP 122 Query: 128 LMFNTILVIIPAEKRGAAMGLIGLVIMVAPAIGPTIAGLLIESLSWHWIFWLSLPFLVIA 187 LMFNTIL+I P KRG+AMG+IGLVIM APA+GPTI+GL++E+L+W+WIFW+SLPFL+IA Sbjct: 123 LMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILENLTWNWIFWISLPFLIIA 182 Query: 188 LISGIFFMQNVTEVTKPKIDXXXXXXXXXXXXXXXXXXXXXXEAEGWGSPKXXXXXXXXX 247 L+ G+ FMQNV+ VTKPKID E GWGS Sbjct: 183 LLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGE-NGWGSATVLVSIIVGG 241 Query: 248 XXXXXXXXRQMTMKQPMINLRAFKFPMFVVGVLMVFICMMVILSSMLILPMYLQQGQGYS 307 RQ+TM++P+++L+ FK+PMF +G+++VFI M+ILS+M++LP+YLQ + Sbjct: 242 IALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALA 301 Query: 308 AFKAXXXXXXXXXXXXXMSPIMGRLFDKYGPKWLVIPGLIIVAVSLWFFSSITATSTVVF 367 AF A MSP GRLFD YGP+ LVIPG I+ ++L+F + I ++ + Sbjct: 302 AFSAGLVLLPGGVLNGLMSPFTGRLFDAYGPRALVIPGFIVAVIALFFLTRIEVGTSALT 361 Query: 368 VIVLHSALMVGVSMIFMPAQTNGINQLPMELYPDGTAIMNTLQQVAGAIGTALAVSIMTA 427 +IVLHS LM+G+SM+ MPAQTNG+NQLP +LYPDGTAIMNTLQQV+GAIGTA+A++IM+A Sbjct: 362 IIVLHSVLMIGISMVMMPAQTNGLNQLPPKLYPDGTAIMNTLQQVSGAIGTAVAITIMSA 421 Query: 428 GSKSYMESVSNPSDPANLLPAFTQGVQNAFVFGMAMAIIGLVVAFFLKR 476 G K+YME+ P ++ + T G+QNAFVFG+ MA IGL+ + F+++ Sbjct: 422 GQKAYMETAQG-VGPEQMIASLTAGIQNAFVFGLIMACIGLLCSLFIRK 469 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 268 bits (684), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 140/467 (29%), Positives = 267/467 (57%), Gaps = 10/467 (2%) Query: 17 KTMPILISLLLAGFIGMFSETALNVALSDLMKVLDITPATAQWLTTAYLLTLGILVPISG 76 K ILI +L+ F+ + ++T +NVAL +MK IT + QWL+T ++L G+++P++ Sbjct: 9 KRNGILIVMLMGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNGVMIPMTA 68 Query: 77 LLLQWFSTRQLFIAALSFSILGTFLAAMSPNFEFLLIARVVQAMGTALLLPLMFNTILVI 136 L++ F+TRQL++ A+ +GT + + ++ L+ R+VQA+G +++PL+ +L + Sbjct: 69 FLIERFTTRQLYLFAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLLTVVVLNL 128 Query: 137 IPAEKRGAAMGLIGLVIMVAPAIGPTIAGLLIESLSWHWIFWLSLPFLVIALISGIFFMQ 196 P E+RG AMGLIGL + APAIGPT++G +++ W +F++ +PF ++ ++ IF ++ Sbjct: 129 FPMERRGRAMGLIGLAMNFAPAIGPTLSGWIVQQYDWRNLFFIIIPFAILDIVVAIFLLK 188 Query: 197 NVTEVTKPKIDXXXXXXXXXXXXXXXXXXXXXXEAEGWGSPKXXXXXXXXXXXXXXXXXR 256 NV + T PK+D + + W + K Sbjct: 189 NVGKRTFPKLDILGVIMSTVGFGSLLLGFSNAGDHD-WLTWKVAGFIILGLVVLGIFIRY 247 Query: 257 QMTMKQPMINLRAFKFPMFVVGVLMVFICMMVILSSMLILPMYLQQGQGYSAFKAXXXXX 316 Q + K P++N + FK+P F + + F +M + ML+LP++LQ +G+S ++ Sbjct: 248 QSSNKAPLLNFKVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLESGLVLL 307 Query: 317 XXXXXXXXMSPIMGRLFDKYGPKWLVIPGLIIVAVSLWFFSSITATSTVVFVIVLHSALM 376 +SP+ G +FD++G K+L + GLII+A S + F+++ ++T+ F+I++ + Sbjct: 308 PGALVTAILSPVTGVMFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTFIIIVQTIRS 367 Query: 377 VGVSMIFMPAQTNGINQLPMELYPDGTAIMNTLQQVAGAIGTALAVSIMTAGSKSYMESV 436 G++M+ MP QT +N LP++L G+A+ NT++QVAG+IGTA +++M+ + S+ + + Sbjct: 368 AGMAMVMMPLQTAALNSLPLKLASHGSAMFNTMRQVAGSIGTAALITVMSKSAASFADKL 427 Query: 437 -------SNPSDPANLLPAFTQGVQNAFVFGMAMAIIGLVVAFFLKR 476 ++ AN G++ AF+ ++++ ++A F+++ Sbjct: 428 GPADVIGKTKTEVAN--HVLIHGIETAFLVAGILSVVACILALFIQK 472 >SPy0570 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 455 Score = 167 bits (424), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 131/468 (27%), Positives = 212/468 (45%), Gaps = 25/468 (5%) Query: 5 MTGKKQTEDRKFKTMPILISLLLAGFIGMFSETALNVALSDLMKVLDITPATAQWLTTAY 64 M+ +QT ++ T+ +I++ L GF G+ SET++NV LM V + + QW+TT Y Sbjct: 1 MSHHQQTVSKQ--TIMAIIAIALIGFSGILSETSMNVTFPTLMSVYQLPLNSLQWMTTIY 58 Query: 65 LLTLGILVPISGLLLQWFSTRQLFIAALSFSILGTFLAAMSPNFEFLLIARVVQAMGTAL 124 LL + I++ S L + R LF A GT LA ++ +F +L+AR+ Q +GT L Sbjct: 59 LLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSFAIMLLARIFQGIGTGL 118 Query: 125 LLPLMFNTILVIIPAEKRGAAMGLIGLVIMVAPAIGPTIAGLLIESLSWHWIFWLSLPFL 184 ++P MFN IL +P K G MG GL+I +APA GPT G +I SW WIF LP Sbjct: 119 VMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGFMISHFSWQWIFICILPVP 178 Query: 185 VIALISGIFFMQNVTEVTKPKIDXXXXXXXXXXXXXXXXXXXXXXEAEGWGSPKXXXXXX 244 +IA I +++++ K D GS Sbjct: 179 LIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLAITSLEN----GSVN-LYYLG 233 Query: 245 XXXXXXXXXXXRQMTMKQPMINLRAFKFPMFVVGVLMVFICMMVILSSMLILPMYLQQGQ 304 + +T KQP +++R K P G++ F+ ++ L + P ++ + Sbjct: 234 LFILSFILFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQLINLGINFLTPNFIVMEK 293 Query: 305 GYSAFKAXXXXXXXXXXXXXMSPIMGRLFDKYGPKWLVIPGLIIVAVSLWFFSSITATST 364 ++ +A ++P G+L+D+ G + + G + ++SL + T Sbjct: 294 IANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLGNALFSLSLIIMTLQTRHFM 353 Query: 365 VVFVIVLHSALMVGVSMIFMPAQTNGINQLPMELYPDGTAIMNTLQQVAGAIGTALAVSI 424 ++ +L+ G +M F + I +LP E D TAI +QQ AGA+GTA+A I Sbjct: 354 LLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADATAIFQMMQQFAGALGTAMASLI 413 Query: 425 MTAGSKSYMESVSNPSDPANLLPAFTQGVQNAFVFGMAMAIIGLVVAF 472 AN FT GVQ+ ++ A++ + F Sbjct: 414 ------------------ANSQAEFTSGVQSVYLLFTIFALLDFIFFF 443 >HI0897 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 510 Score = 134 bits (337), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 102/418 (24%), Positives = 192/418 (45%), Gaps = 4/418 (0%) Query: 30 FIGMFSETALNVALSDLMKVLDITPATAQWLTTAYLLTLGILVPISGLLLQWFSTRQLFI 89 F+ + T NVA+ + L + + W+ T++ + I +PI+G L + F +LF+ Sbjct: 28 FMQVLDSTIANVAIPTIAGDLGASFSQGTWVITSFGVANAISIPITGWLAKRFGEVRLFL 87 Query: 90 AALSFSILGTFLAAMSPNFEFLLIARVVQAMGTALLLPLMFNTILVIIPAEKRGAAMGLI 149 + ++ ++L ++ + E L+I RV+Q ++PL + +L P EKRG A+ Sbjct: 88 VSTFLFVVSSWLCGIADSLEALIIFRVIQGAVAGPVIPLSQSLLLNNYPPEKRGMALAFW 147 Query: 150 GLVIMVAPAIGPTIAGLLIESLSWHWIFWLSLPF-LVIALISGIFFMQNVTEVTKPKIDX 208 + I+VAP GP + G + +++ W WIF++++P L + LIS +E+ ID Sbjct: 148 SMTIVVAPIFGPILGGWISDNIHWGWIFFINVPIGLSVVLISWKILGSRESEIVHQPIDK 207 Query: 209 XXXXXXXXXXXXXXXXXXXXXEAEGWGSPKXXXXXXXXXXXXXXXXXRQMTMKQPMINLR 268 E + + S + ++T P++++ Sbjct: 208 VGLVLLVLGVGCLQLMLDQGREQDWFNSNEIIILAVVAVVCLIALVIWELTDDNPVVDIS 267 Query: 269 AFKFPMFVVGVLMVFICMMVILSSMLILPMYLQQGQGYSAFKAXXXXXXXXXXXXXMSPI 328 F F VG L + ++ L S++++P+ LQQ Y+A A +SPI Sbjct: 268 LFHSRNFSVGCLCTSLAFLIYLGSVVLIPLLLQQVFHYTATWAGLAASPVGLFPILLSPI 327 Query: 329 MGRLFDKYGPKWLVIPGLIIVAVSLWFFSSITATSTVVFVIVLHSALMVG--VSMIFMPA 386 +GR K + LV I+ A++ ++ ++T ++ FV V L+ G VS FMP Sbjct: 328 IGRFGYKIDMRILVTISFIVYAITF-YWRAVTFEPSMTFVDVALPQLVQGLAVSCFFMPL 386 Query: 387 QTNGINQLPMELYPDGTAIMNTLQQVAGAIGTALAVSIMTAGSKSYMESVSNPSDPAN 444 T ++ LP +++ N L+ +AG++GT+L + + ++ +P N Sbjct: 387 TTITLSGLPAHKMASASSLFNFLRTLAGSVGTSLTTFMWYNREAVHHTQLTEHINPYN 444 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,621,313 Number of extensions: 1038782 Number of successful extensions: 4654 Number of sequences better than 1.0e-05: 88 Number of HSP's gapped: 4638 Number of HSP's successfully gapped: 100 Length of query: 488 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 378 Effective length of database: 25,691,691 Effective search space: 9711459198 Effective search space used: 9711459198 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 118 (50.1 bits)