BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0031.p (455 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll2278 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 439 e-123 SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene te... 384 e-106 BS_ytnJ [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 335 8e-92 lin2437 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 334 2e-91 mll5137 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 327 3e-89 >mll2278 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 439 bits (1129), Expect = e-123, Method: Compositional matrix adjust. Identities = 0/440 (0%), Positives = 0/440 (0%), Gaps = 4/440 (0%) Query: 7 QLALGAMMFFPAGEHISSWRHPGSEAAALLDFDYYKRIVQTAERGRFDMFFYADELYVWD 66 + + + + + A + Q + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 67 RFESGISHANSIRPEPFTLLSALAAVTTHIGLAATISTTYNDPYHIARKLASLDHLSGGR 126 + L + V+ + A D L + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 127 AAWNIVTSQTDEEARNFGKERHLQHELRYERAKEFVAVTQGLWDSWEEDACVFDKESGYF 186 + + + + A + L + + S Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 187 ADKDKLHNLDFEGKHFKVKGPLNITRPPQGYPVLIQAGASEAGKEVAAATAEIVFAPGGT 246 A + + + + + V + T Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 247 LAEGRALYADIKGRLAKYGRTADELKIFPALMPVIGITEEDAAEKLSVIENLTPDRLALD 306 T + + + + R Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFV 302 Query: 307 LLSHYIGTDVSSYXXXXXXXXXXXXXGFNQSKTGLLKIRSILEQENLTLLQLAKRVAVKR 366 + T + + ++ + Sbjct: 303 ASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYTYPAP- 361 Query: 367 G---IAGTPEQIADHIEEWFKSKAADGFNIAFTHLPGALDDFVNLVVPILQQRGLLRTEY 423 Sbjct: 362 421 Query: 424 EGTTLRAHLGLNTPASRYAK 443 Sbjct: 422 441 >SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 384 bits (986), Expect = e-106, Method: Compositional matrix adjust. Identities = 0/444 (0%), Positives = 0/444 (0%), Gaps = 12/444 (2%) Query: 4 VKRQLALGAMMFFPAG-EHISSWRHPGSEAAALLDFDYYKRIVQTAERGRFDMFFYADEL 62 ++ + + + + A + Q + Sbjct: 1 MQYHRIPHS--SLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPR 58 Query: 63 YVWDRFESGISHANSIRPEPFTLLSALAAVTTHIGLAATISTTYNDPYHIARKLASLDHL 122 N + +A+ + + +L Sbjct: 59 PETQGLTET-YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDS 117 Query: 123 SGGRAAWNIVTSQTDEEARNFGKERHLQHELRYERAKEFVAVTQGLWDSWEEDACVFDKE 182 + Q R L + + T ++ + Sbjct: 118 LKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIG 177 Query: 183 SGYFADKDKLHNLDFEGKHFKVKGPLNITRPPQGYPVLIQAGASEAGKEVAAATAEIVFA 242 + L KH + A + E A Sbjct: 178 VSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLG 237 Query: 243 PGGTLAEGRALYADIKGRLAKYGRTADELKIFPALMPVIGITEEDAAEKLSVIENLTPDR 302 G + R + R + L R Sbjct: 238 FGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVR 297 Query: 303 LALDLLSHYIG--TDVSSYXXXXXXXXXXXXXGFNQSKTGLLKIRSILEQENLTLLQLAK 360 + S +G T + Sbjct: 298 RQPFVASTLLGATTMDQLKTNIESLHLELSEDVL-AEIEAV-- 354 Query: 361 RVAVKRG---IAGTPEQIADHIEEWFKSKAADGFNIAFTHLPGALDDFVNLVVPILQQRG 417 Sbjct: 355 414 Query: 418 LLRTEYEGTTLRAHLGLNTPASRY 441 Sbjct: 415 438 >BS_ytnJ [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 335 bits (859), Expect = 8e-92, Method: Compositional matrix adjust. Identities = 0/431 (0%), Positives = 0/431 (0%), Gaps = 7/431 (1%) Query: 8 LALGAMMFFPAGEHISSWRHPGSEAAALLDFDYYKRIVQTAERGRFDMFFYADELYVWDR 67 + G ++ SEA A DY Sbjct: 7 PHSSLEVS-TLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG-- 63 Query: 68 FESGISHANSIRPEPFTLLSALAAVTTHIGLAATISTTYNDPYHIARKLASLDHLSGGRA 127 + N + + + A D + + H S R Sbjct: 64 -LTETYVGNW-LAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 128 AWNIVTSQTDEEARNFGKERHLQHELRYERAKEFVAVTQGLWDSWEEDACVFDKESGYFA 187 + + + + A + + + A Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 188 DKDKLHNLDFEGKHFKVKGPLNITRPPQGYPVLIQAGASEAGKEVAAATAEIVFAPGGTL 247 + + + I P + G +E + GTL Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 248 AEGRALYADIKGRLAKYGRTADELKIFPALMPVIGITEEDAAEKLSVIENLTPDRLALDL 307 A G V + L + Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPF 301 Query: 308 LSHYIGTDVSSYXXXXXXXXXXXXXGFNQSKTGLLKIRSILEQENLTLLQLAKRVAVKRG 367 Sbjct: 302 361 Query: 368 I-AGTPEQIADHIEEWFKSKAADGFNIAFTHLPGALDDFVNLVVPILQQRGLLRTEYEGT 426 Sbjct: 362 - 420 Query: 427 TLRAHLGLNTP 437 Sbjct: 421 431 >lin2437 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 334 bits (856), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 0/439 (0%), Positives = 0/439 (0%), Gaps = 7/439 (1%) Query: 5 KRQLALGAMMFFPAGEHISSWRHPGSEAAALLDFDYYKRIVQTAERGRFDMFFYADELYV 64 R + G ++ SEA A DY Sbjct: 4 HRIPHSSLEVS-TLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 65 WDRFESGISHANSIRPEPFTLLSALAAVTTHIGLAATISTTYNDPYHIARKLASLDHLSG 124 + N + + + A D + + H S Sbjct: 63 G---LTETYVGNW-LAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSL 118 Query: 125 GRAAWNIVTSQTDEEARNFGKERHLQHELRYERAKEFVAVTQGLWDSWEEDACVFDKESG 184 R + + + + A + + + A Sbjct: 119 KRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 Query: 185 YFADKDKLHNLDFEGKHFKVKGPLNITRPPQGYPVLIQAGASEAGKEVAAATAEIVFAPG 244 + + + I P + G +E + Sbjct: 179 SNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 245 GTLAEGRALYADIKGRLAKYGRTADELKIFPALMPVIGITEEDAAEKLSVIENLTPDRLA 304 GTL A G V + L + Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRR 298 Query: 305 LDLLSHYIGTDVSSYXXXXXXXXXXXXXGFNQSKTGLLKIRSILEQENLTLLQLAKRVAV 364 Sbjct: 299 358 Query: 365 -KRGIAGTPEQIADHIEEWFKSKAADGFNIAFTHLPGALDDFVNLVVPILQQRGLLRTEY 423 Sbjct: 359 - 417 Query: 424 EGTTLRAHLGLNTPASRYA 442 Sbjct: 418 436 >mll5137 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 327 bits (838), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 0/444 (0%), Positives = 0/444 (0%), Gaps = 46/444 (10%) Query: 7 QLALGAMMFFPAGEHISSWRHPGSEAAALLDFDYYKRIVQTAERGRFDMFFYADELYVWD 66 + + + + + + A + Q + Sbjct: 4 HRIPHSSLE-VSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPP----- 57 Query: 67 RFESGISHAN---------SIRPEPFTLLSALAAVTTHIGLAATISTTYNDPYHIARKLA 117 + + L+ A I R+ Sbjct: 58 ---RPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREAL 114 Query: 118 SLDHLSGGRAAWNIVTSQTDEEARNFGKERHLQHELRYERAKEFVAVTQGLWDSWEEDAC 177 ++ + N + + Sbjct: 115 HDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQ------ 168 Query: 178 VFDKESGYFADKDKLHNLDFEGKHFKVKGPLNITRPPQGYPVLIQAGASEAGKEVAAATA 237 + + + R + Sbjct: 169 ---RAGKI------------RYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNR 213 Query: 238 EIVFAPGGTLAEGRALYADIKGRLAKYGRTADELKIFPALMPVIGITEEDAAEKLSVIEN 297 PA + + Sbjct: 214 SFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKA 273 Query: 298 LTPDRLALDLLSHYIG-TDVSSYXXXXXXXXXXXXXGFNQSKTGLLKIRSILEQENLTLL 356 + ++ + E + Sbjct: 274 VAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATT-MDQLKTNIESL--HLELSEDV 330 Query: 357 QLAKRVAVKRG---IAGTPEQIADHIEEWFKSKAADGFNIAFTHLPGALDDFVNLVVPIL 413 + Sbjct: 331 LAEIEAVHQVYTYP 390 Query: 414 QQRGLLRTEYEGTTLRAHLGLNTP 437 Sbjct: 391 414 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,189,612 Number of extensions: 1437963 Number of successful extensions: 2726 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 2674 Number of HSP's successfully gapped: 33 Length of query: 455 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 345 Effective length of database: 25,691,691 Effective search space: 8863633395 Effective search space used: 8863633395 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)