BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0028.p (166 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_opuCB [E] COG1174 ABC-type proline/glycine betaine transport ... 134 4e-32 SA0678_1 [E] COG1174 ABC-type proline/glycine betaine transport ... 128 4e-30 BS_opuBB [E] COG1174 ABC-type proline/glycine betaine transport ... 127 7e-30 lin1466 [E] COG1174 ABC-type proline/glycine betaine transport s... 126 2e-29 lin1461_1 [E] COG1174 ABC-type proline/glycine betaine transport... 124 6e-29 >BS_opuCB [E] COG1174 ABC-type proline/glycine betaine transport systems, permease component Length = 217 Score = 134 bits (338), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 66/143 (46%), Positives = 100/143 (69%), Gaps = 2/143 (1%) Query: 1 MNHVWDTLVADWPMILDKTGEHMYLSLVSVCIAGLIAIPLGIYLTSRKSLANSIITIISA 60 MN + L + +L KTGEH+Y+SL++V + ++A+PLG+ LT K A ++I ++ Sbjct: 1 MNQMMTFLQTNGGELLYKTGEHLYISLIAVVLGIIVAVPLGVALTRMKKGAGAVIGFVNI 60 Query: 61 VQTIPSLALFGFMIPLVGIGTTPAXXXXALTLYALLPMLQNTYVGISGVNPALIEAGTGM 120 VQT+PSLA+ F IPL+G+G PA AL Y++LP+L+NTY GI GVN L+E+G G+ Sbjct: 61 VQTLPSLAILAFFIPLLGVGKVPA--IVALFFYSVLPILRNTYTGIKGVNKNLLESGKGI 118 Query: 121 GMTRWQMMTMIQLPIARSIIMAG 143 GMT W+ + ++++P+A IIMAG Sbjct: 119 GMTGWEQIRLVEIPLAIPIIMAG 141 >SA0678_1 [E] COG1174 ABC-type proline/glycine betaine transport systems, permease component Length = 234 Score = 128 bits (321), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 67/143 (46%), Positives = 95/143 (66%), Gaps = 2/143 (1%) Query: 1 MNHVWDTLVADWPMILDKTGEHMYLSLVSVCIAGLIAIPLGIYLTSRKSLANSIITIISA 60 M + +D L + EH+ +S +++ IA IA+PLGI LT K+++ ++ I + Sbjct: 1 MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILLTKTKTISEIVMNIAAI 60 Query: 61 VQTIPSLALFGFMIPLVGIGTTPAXXXXALTLYALLPMLQNTYVGISGVNPALIEAGTGM 120 +QTIPSLAL G MIPL GIG PA AL +YALLP+L+NTY GI V+P+LIEA G+ Sbjct: 61 LQTIPSLALLGLMIPLFGIGRVPA--IIALVVYALLPILRNTYTGIKEVDPSLIEAAKGI 118 Query: 121 GMTRWQMMTMIQLPIARSIIMAG 143 GM ++ +T ++LPIA +IMAG Sbjct: 119 GMKPFRRLTKVELPIAMPVIMAG 141 >BS_opuBB [E] COG1174 ABC-type proline/glycine betaine transport systems, permease component Length = 217 Score = 127 bits (319), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 65/143 (45%), Positives = 99/143 (69%), Gaps = 2/143 (1%) Query: 1 MNHVWDTLVADWPMILDKTGEHMYLSLVSVCIAGLIAIPLGIYLTSRKSLANSIITIISA 60 M+H+ L + +L KT EH+ +SL++V + L+A+PLG+ LT K A +II I++ Sbjct: 1 MHHIVQFLQTNGGELLYKTYEHITISLIAVILGVLVAVPLGVVLTRMKKGAGTIIGIVNI 60 Query: 61 VQTIPSLALFGFMIPLVGIGTTPAXXXXALTLYALLPMLQNTYVGISGVNPALIEAGTGM 120 +QT+PSLA+ F IPL+G+G PA AL Y++LP+L+NTY GI GVN L+E+G G+ Sbjct: 61 IQTLPSLAILAFFIPLLGVGKVPA--IVALFFYSVLPILRNTYTGIRGVNKNLLESGKGI 118 Query: 121 GMTRWQMMTMIQLPIARSIIMAG 143 GMT + + +++LP+A +IMAG Sbjct: 119 GMTPAEQVRLVELPLAAPVIMAG 141 >lin1466 [E] COG1174 ABC-type proline/glycine betaine transport systems, permease component Length = 218 Score = 126 bits (316), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 60/129 (46%), Positives = 96/129 (74%), Gaps = 2/129 (1%) Query: 15 ILDKTGEHMYLSLVSVCIAGLIAIPLGIYLTSRKSLANSIITIISAVQTIPSLALFGFMI 74 +L +T +H+++SL +V + +A+P+GI LT +AN +I ++S +QT+PSLA+ F+I Sbjct: 15 LLVQTWQHLFISLSAVILGIAVAVPVGILLTRSPKVANFVIGVVSVLQTVPSLAILAFII 74 Query: 75 PLVGIGTTPAXXXXALTLYALLPMLQNTYVGISGVNPALIEAGTGMGMTRWQMMTMIQLP 134 P +G+GT PA AL +YALLP+L+NT++G+ GV+ LIE+G GMGMT WQ++ +++P Sbjct: 75 PFLGVGTLPA--IIALFIYALLPILRNTFIGVRGVDKNLIESGRGMGMTNWQLIINVEIP 132 Query: 135 IARSIIMAG 143 + S+IMAG Sbjct: 133 NSISVIMAG 141 >lin1461_1 [E] COG1174 ABC-type proline/glycine betaine transport systems, permease component Length = 205 Score = 124 bits (311), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 74/143 (51%), Positives = 95/143 (66%), Gaps = 2/143 (1%) Query: 1 MNHVWDTLVADWPMILDKTGEHMYLSLVSVCIAGLIAIPLGIYLTSRKSLANSIITIISA 60 MN + DT + +H+ +S VS+ IA LIA+PLGIYLT K LA II + + Sbjct: 1 MNTLLDTFAVRKDELFTALVQHIQISFVSLFIAVLIALPLGIYLTRHKRLAEPIIQVAAI 60 Query: 61 VQTIPSLALFGFMIPLVGIGTTPAXXXXALTLYALLPMLQNTYVGISGVNPALIEAGTGM 120 QTIPSLAL G +IPLVGIG PA AL +YALLP+L+NTY GI V+PAL+EA M Sbjct: 61 FQTIPSLALLGLLIPLVGIGIVPA--IIALVIYALLPILRNTYTGIKEVDPALVEASRAM 118 Query: 121 GMTRWQMMTMIQLPIARSIIMAG 143 GM +W+ + +QLP+A +IMAG Sbjct: 119 GMNKWKRLYKVQLPLAMPVIMAG 141 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.139 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,124,006 Number of extensions: 433973 Number of successful extensions: 3298 Number of sequences better than 1.0e-05: 83 Number of HSP's gapped: 3271 Number of HSP's successfully gapped: 84 Length of query: 166 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 68 Effective length of database: 27,075,699 Effective search space: 1841147532 Effective search space used: 1841147532 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 112 (47.8 bits)