BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0025.p (274 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll7342 [S] COG2343 Uncharacterized protein conserved in bacteria 179 5e-45 mll2277 [S] COG2343 Uncharacterized protein conserved in bacteria 171 1e-42 Rv1056 [S] COG2343 Uncharacterized protein conserved in bacteria 139 4e-33 MT1085 [S] COG2343 Uncharacterized protein conserved in bacteria 139 4e-33 Rv0140 [S] COG2343 Uncharacterized protein conserved in bacteria 72 8e-13 >mll7342 [S] COG2343 Uncharacterized protein conserved in bacteria Length = 247 Score = 179 bits (454), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 95/238 (39%), Positives = 139/238 (58%), Gaps = 2/238 (0%) Query: 24 IAKKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRNDLLTASDRKSFFPLKGEAS 83 + K++ V+ I+A++T L+L+E LPVYYFP DVR + L S + P KG A+ Sbjct: 3 MQKRLRVQVGSMIVADTTKALVLYESDHLPVYYFPMSDVREEFLLPSRTTTESPFKGVAT 62 Query: 84 YWSLQLGDEISENAAWSYEHPIPGREDIAGYYAFYWNAVDGWXXXXXXXXXXXXXDPYKR 143 ++SL G + E+AAW Y P+ G D++GY +FYW + W DP++R Sbjct: 63 HYSLNTGITLVEDAAWRYLDPVKGSPDLSGYMSFYWPKMTHWYEEDEEIFVHAR-DPFRR 121 Query: 144 IDALRSSRHIEIYLNGVKLADSTRPVILFETGVPVRYYLPEDDLNTALLEFSDSKTACPY 203 +D L SSR +++ L+G ++ADS R V LFETG PVR+YLP D + S + CPY Sbjct: 122 VDCLPSSRRVQVILDGEQVADSRRGVFLFETGHPVRHYLPISDTRLDMFAPSRYISRCPY 181 Query: 204 KGHASYRSVRIHDELHKDLVWTYASPIAEIPQIAGLFSFYNERVE-IFVDGKRQPKQA 260 KG ++Y V + H++LVW Y P+ E +I GL F++E V+ I VDG K+A Sbjct: 182 KGISNYYHVTTPQKRHENLVWYYPEPVHEAERIKGLVCFHHELVDKILVDGVDISKEA 239 >mll2277 [S] COG2343 Uncharacterized protein conserved in bacteria Length = 257 Score = 171 bits (432), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 100/253 (39%), Positives = 140/253 (55%), Gaps = 6/253 (2%) Query: 5 ELQEVREQRPQKLNVKTDRIAKKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRN 64 LQ P+ +NV K+V V++ G IA+S +VL+L LP+Y+FP V Sbjct: 6 NLQPREATAPRLVNVLHS--PKRVRVKFGGRTIADSRNVLVLRSNHFLPIYFFPPSSVDA 63 Query: 65 DLLTASDRKSFFPLKGEASYWSLQLGDEISENAAWSYEHPI-PGREDIAGYYAFYWNAVD 123 +L S + + GE +YW + GD + NAAWS+ P +AG AF W AVD Sbjct: 64 SVLKPSLHREPHAVGGETAYWDIDSGDRRAANAAWSFTAPPDENLSPLAGRIAFTWKAVD 123 Query: 124 GWXXXXXXXXXXXXXDPYKRIDALRSSRHIEIYLNGVKLADSTRPVILFETGVPVRYYLP 183 W DPY RID L SS H++++ +G +ADS RPV+LFET +P R+YL Sbjct: 124 QWFEEEEEVFVHAR-DPYARIDVLHSSSHVQVWFDGDIIADSHRPVLLFETHLPTRFYLR 182 Query: 184 EDDLNTALLEFSDSKTACPYKGHASYRSVRIHD-ELHKDLVWTYASPIAEIPQIAGLFSF 242 +D+ L S S T CPYKG ASY S + D + D+ W+Y PI E+P+I GL +F Sbjct: 183 PEDVRLERLTASSSTTRCPYKGIASYWSGLLKDGSVRPDIAWSYRDPIDEMPKIKGLIAF 242 Query: 243 YNERVE-IFVDGK 254 Y + V+ I +DG+ Sbjct: 243 YPQAVDRIHLDGQ 255 >Rv1056 [S] COG2343 Uncharacterized protein conserved in bacteria Length = 254 Score = 139 bits (350), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 80/207 (38%), Positives = 116/207 (56%), Gaps = 6/207 (2%) Query: 53 PVYYFPAGDVRNDLLTASDRKSFFPLKGEASYWSLQLGDEISENAAWSYEHPIPGREDIA 112 P YY P DVR + L + L G + SL + +AA ++ + G +A Sbjct: 47 PQYYIPLADVRMEFLRDENHPQRVQL-GPSRLHSLVSAGQTHRSAARVFD--VDGDSPVA 103 Query: 113 GYYAFYWNAVDGWXXXXXXXXXXXXXDPYKRIDALRSSRHIEIYLNGVKLADSTRPVILF 172 G F W+ + + +PY+R DALRS RH+ + L+G+ LAD+ PV+LF Sbjct: 104 GTVRFNWDPLRWFEEDEPIYGHPR--NPYQRADALRSHRHVRVELDGIVLADTRSPVLLF 161 Query: 173 ETGVPVRYYLPEDDLNTALLEFSDSKTACPYKGHAS-YRSVRIHDELHKDLVWTYASPIA 231 ETG+P RYY+ D+ LE + ++T CPYKG S Y SVR+ D +H+DL WTY P+ Sbjct: 162 ETGIPTRYYIDPADIAFEHLEPTSTQTLCPYKGTTSGYWSVRVGDAVHRDLAWTYHYPLP 221 Query: 232 EIPQIAGLFSFYNERVEIFVDGKRQPK 258 + IAGL +FYNE+V++ VDG P+ Sbjct: 222 AVAPIAGLVAFYNEKVDLTVDGVALPR 248 Score = 65.5 bits (158), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 35/102 (34%), Positives = 54/102 (52%), Gaps = 1/102 (0%) Query: 23 RIAKKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRNDLLTASDRKSFFPLKGEA 82 R + V V G ++A++ +LL E G YY D+ + L + ++ P KG Sbjct: 137 RSHRHVRVELDGIVLADTRSPVLLFETGIPTRYYIDPADIAFEHLEPTSTQTLCPYKGTT 196 Query: 83 S-YWSLQLGDEISENAAWSYEHPIPGREDIAGYYAFYWNAVD 123 S YWS+++GD + + AW+Y +P+P IAG AFY VD Sbjct: 197 SGYWSVRVGDAVHRDLAWTYHYPLPAVAPIAGLVAFYNEKVD 238 >MT1085 [S] COG2343 Uncharacterized protein conserved in bacteria Length = 254 Score = 139 bits (350), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 80/207 (38%), Positives = 116/207 (56%), Gaps = 6/207 (2%) Query: 53 PVYYFPAGDVRNDLLTASDRKSFFPLKGEASYWSLQLGDEISENAAWSYEHPIPGREDIA 112 P YY P DVR + L + L G + SL + +AA ++ + G +A Sbjct: 47 PQYYIPLADVRMEFLRDENHPQRVQL-GPSRLHSLVSAGQTHRSAARVFD--VDGDSPVA 103 Query: 113 GYYAFYWNAVDGWXXXXXXXXXXXXXDPYKRIDALRSSRHIEIYLNGVKLADSTRPVILF 172 G F W+ + + +PY+R DALRS RH+ + L+G+ LAD+ PV+LF Sbjct: 104 GTVRFNWDPLRWFEEDEPIYGHPR--NPYQRADALRSHRHVRVELDGIVLADTRSPVLLF 161 Query: 173 ETGVPVRYYLPEDDLNTALLEFSDSKTACPYKGHAS-YRSVRIHDELHKDLVWTYASPIA 231 ETG+P RYY+ D+ LE + ++T CPYKG S Y SVR+ D +H+DL WTY P+ Sbjct: 162 ETGIPTRYYIDPADIAFEHLEPTSTQTLCPYKGTTSGYWSVRVGDAVHRDLAWTYHYPLP 221 Query: 232 EIPQIAGLFSFYNERVEIFVDGKRQPK 258 + IAGL +FYNE+V++ VDG P+ Sbjct: 222 AVAPIAGLVAFYNEKVDLTVDGVALPR 248 Score = 65.5 bits (158), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 35/102 (34%), Positives = 54/102 (52%), Gaps = 1/102 (0%) Query: 23 RIAKKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRNDLLTASDRKSFFPLKGEA 82 R + V V G ++A++ +LL E G YY D+ + L + ++ P KG Sbjct: 137 RSHRHVRVELDGIVLADTRSPVLLFETGIPTRYYIDPADIAFEHLEPTSTQTLCPYKGTT 196 Query: 83 S-YWSLQLGDEISENAAWSYEHPIPGREDIAGYYAFYWNAVD 123 S YWS+++GD + + AW+Y +P+P IAG AFY VD Sbjct: 197 SGYWSVRVGDAVHRDLAWTYHYPLPAVAPIAGLVAFYNEKVD 238 >Rv0140 [S] COG2343 Uncharacterized protein conserved in bacteria Length = 126 Score = 72.4 bits (176), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 35/94 (37%), Positives = 53/94 (56%), Gaps = 1/94 (1%) Query: 26 KKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRNDLLTASDRKSFFPLKGEASYW 85 ++V VR GE++A++ L L E V Y P DV D L ++ ++ P KGEASY+ Sbjct: 22 RRVQVRVNGEVVADTAAALCLQEASYPAVQYIPLADVVQDRLIRTETSTYCPFKGEASYY 81 Query: 86 SLQL-GDEISENAAWSYEHPIPGREDIAGYYAFY 118 S+ +I ++ W+YE+P P IAG+ A Y Sbjct: 82 SVTTDAGDIVDDVMWTYENPYPAVAAIAGHVACY 115 Score = 62.8 bits (151), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 33/104 (31%), Positives = 58/104 (55%), Gaps = 1/104 (0%) Query: 149 SSRHIEIYLNGVKLADSTRPVILFETGVPVRYYLPEDDLNTALLEFSDSKTACPYKGHAS 208 ++R +++ +NG +AD+ + L E P Y+P D+ L +++ T CP+KG AS Sbjct: 20 TNRRVQVRVNGEVVADTAAALCLQEASYPAVQYIPLADVVQDRLIRTETSTYCPFKGEAS 79 Query: 209 YRSVRIH-DELHKDLVWTYASPIAEIPQIAGLFSFYNERVEIFV 251 Y SV ++ D++WTY +P + IAG + Y ++ EI + Sbjct: 80 YYSVTTDAGDIVDDVMWTYENPYPAVAAIAGHVACYPDKAEISI 123 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,918,499 Number of extensions: 790337 Number of successful extensions: 5176 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 5160 Number of HSP's successfully gapped: 21 Length of query: 274 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 169 Effective length of database: 26,268,361 Effective search space: 4439353009 Effective search space used: 4439353009 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)