BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0024.p (375 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0797 [KG] COG1940 Transcriptional regulator/sugar kinase 130 3e-30 FN1164 [KG] COG1940 Transcriptional regulator/sugar kinase 126 6e-29 mlr7234 [KG] COG1940 Transcriptional regulator/sugar kinase 125 1e-28 BH1094 [KG] COG1940 Transcriptional regulator/sugar kinase 124 4e-28 SP0668 [KG] COG1940 Transcriptional regulator/sugar kinase 120 3e-27 >BH0797 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 293 Score = 130 bits (328), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 96/315 (30%), Positives = 150/315 (47%), Gaps = 29/315 (9%) Query: 53 IGVDLGGTNIKAALFDASFNAIGEISLSTEAAKGPAHVLSRIRLAVQQLTEDKGIPLQSI 112 +G+DLGGT IKAAL + I TEAA+GP V++++ +++T+ Q Sbjct: 3 VGIDLGGTKIKAALVSDAGEIISVQECPTEAAQGPEEVMNKMMSLTEKVTDH-----QPF 57 Query: 113 TCMGLGIPGLLDPEEGMSLFSPNFPGWEHIHIVNEMKLYYDFPIFIDNDVRVNLYGEWQY 172 +G+G PG L EG L PN PGW+HIH+V+ + + P+ +DND V E Sbjct: 58 AGIGIGAPGPLSSTEGTILSPPNLPGWDHIHLVDRFQEQFQCPVKLDNDANVAALAEALL 117 Query: 173 GAARGYKNVVXXXXXXXXXXXIVSDGKVVYGTTFSAGEIGHMNMFXXXXXXXXXXXXXXX 232 G+ +G+ +V V DG +V+G + AGEIG+M Sbjct: 118 GSGQGFTSVFYLTISTGIGGGYVLDGSIVHGASDYAGEIGNM-------------IVQPN 164 Query: 233 XYVSA---VGMVNTFTEKLAEGRPSIIQAWTNGNNGQITAQMISEAYDLEDPLAAEVMHE 289 Y A G + A GR + + G ++ Q+ +D++ L E M Sbjct: 165 GYQHANLNPGSLEGLASGTAIGRMARERFGVEGGTREVFDQIRRGDHDMQR-LVEEAMD- 222 Query: 290 TGVLLGFGLANVINLLNPEVIVVGGGMSXXXXAGERLLHSVRETVKDHALKLSGSKCSIV 349 L G+AN+ + +NP+V V+GGG+ A + +L V+E V + +IV Sbjct: 223 ---YLAIGIANIAHTINPDVFVLGGGV---MNADDLILPIVKEKVSRYLYPGLAQSTTIV 276 Query: 350 QAELGSRAGTLGAAV 364 +A+LG +G LGAA+ Sbjct: 277 KAKLGGDSGVLGAAM 291 >FN1164 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 315 Score = 126 bits (316), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 89/315 (28%), Positives = 153/315 (48%), Gaps = 5/315 (1%) Query: 48 MKTYTIGVDLGGTNIKAALFDASFNAIGEISLSTEAAKGPAHVLSRIRLAVQQLTEDKGI 107 MK Y IG+DLGGTN K + D+ N I + T + + L RI ++L +K I Sbjct: 1 MKHY-IGIDLGGTNTKIGVVDSEGNLINSKIIKTHSHQNVDKTLERIWETAKKLILEKEI 59 Query: 108 PLQSITCMGLGIPGLLDPEEGMSLFSPNFPGWEHIHIVNEMKLYYDFPIFIDNDVRVNLY 167 PL S+ +G+GIPG + + + F+ NF +++++ +M+ I+ND + Sbjct: 60 PLFSVVGIGIGIPGPVKNQSTVGFFA-NFDWEKNLNLKEKMEKLSGIETRIENDANIIAQ 118 Query: 168 GEWQYGAARGYKNVVXXXXXXXXXXXIVSDGKVVYGTTFSAGEIGHMNMFXXXXXXXXXX 227 GE +GAA+G K+ + I +G ++ G + GEIGHM + Sbjct: 119 GEAIFGAAKGKKSSITIAIGTGIGGGIFYNGNLISGMSGVGGEIGHMKVVKDGKTCGCGQ 178 Query: 228 XXXXXXYVSAVGMVNTFTEKLAEGRPSIIQAWTNGNNGQITAQMISEAYDLEDPLAAEVM 287 Y SA +V E+L +++ NG+ ++ A+ I +A D + +++ Sbjct: 179 NGCFEAYASASSLVKEAKERLKLNEDNLLFKEINGDLDELEAKNIFDAARKGDQFSKDLI 238 Query: 288 HETGVLLGFGLANVINLLNPEVIVVGGGMSXXXXAGERLLHSVRETVKDHALKLSGSKCS 347 L G+ N++N++NPE IV+ GG+S AG+ +L ++E +K + + + Sbjct: 239 EYESDYLALGIGNLLNIINPECIVISGGIS---LAGDEILLPIKEKLKKYTMPPALENLE 295 Query: 348 IVQAELGSRAGTLGA 362 I LG+ AG GA Sbjct: 296 IKIGTLGNEAGVKGA 310 >mlr7234 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 339 Score = 125 bits (314), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 97/326 (29%), Positives = 165/326 (50%), Gaps = 36/326 (11%) Query: 52 TIGVDLGGTNIKAALFDASFNAIGEISLSTEAAKGPAHVLSRIR-LAVQQLTEDKGIPLQ 110 + +DLGGT ++AAL D + ++ T+A GP V+ +I LA E G+ Sbjct: 6 ALAIDLGGTELRAALVDRDGKILAFAAVPTQAQAGPDVVIGQIEALAATVHAEAPGL--- 62 Query: 111 SITCMGLGIPGLLDPEEGMSLFSPNFPGWEHIHIVNEMKLYYDFPIFIDNDVRVNLYGEW 170 +I +G+G PG LDP G+++ P GW+ + + + ++ P+ ++ND GEW Sbjct: 63 AIVGVGVGAPGPLDPLAGIAVGPPTLAGWQDVPLADILERRLGLPVRLENDANAAALGEW 122 Query: 171 QYGAARGYKNVVXXXXXXXXXXXIVSDGKVVYGTTFSAGEIGHMNMFXXXXXXXXXXXXX 230 ++GA G +++V +V+DG++++G A EIGHM + Sbjct: 123 RFGAGHGARSLVFVTVSTGIGGGVVADGRILHGRRGLAAEIGHMTITNEGER-------- 174 Query: 231 XXXYVSAVGMVNTFTEKLAEG-----RPSIIQAWTNGN-------NGQITAQMISEAYDL 278 G+V F E +A G R + + +G+ N ++T + + EA L Sbjct: 175 -----CVCGVVGCF-EAIASGTALGRRANAATSAFDGSTLRRLSANAEVTGRHVVEAARL 228 Query: 279 EDPLAAEVMHETGVLLGFGLANVINLLNPEVIVVGGGMSXXXXAGERLLHSVRE-TVKDH 337 +D LA ++ E LG G N+++L +P+V+VVGGG++ G L+H V E T++ Sbjct: 229 QDDLALALLEEEARWLGVGFTNLLHLYSPDVLVVGGGIAN----GLDLMHPVIEATIRQR 284 Query: 338 ALKLSGSKCSIVQAELGSRAGTLGAA 363 A++ + +VQA+LG AG +GAA Sbjct: 285 AMR-AYRDVPVVQAQLGRHAGLVGAA 309 >BH1094 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 407 Score = 124 bits (310), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 83/313 (26%), Positives = 153/313 (48%), Gaps = 4/313 (1%) Query: 50 TYTIGVDLGGTNIKAALFDASFNAIGEISLSTEAAKGPAHVLSRIRLAVQQLTEDKGIPL 109 ++ IG+D+GG ++A L D + G+ ++ + ++ ++ L E + Sbjct: 81 SFVIGLDVGGYRVRAVLTDLNGEVEGQTTVELSSDLTEETLIDQLIKITVTLFEQCKMQK 140 Query: 110 QSITCMGLGIPGLLDPEEGMSLFSPNFPGWEHIHIVNEMKLYYDFPIFIDNDVRVNLYGE 169 + +G+G+ G++D + GM++F+PNF +I + + +D P+F++ND R GE Sbjct: 141 DKVIGIGIGMHGIVDHQSGMAVFAPNFQ-LTNIDLKTPFESTFDVPVFVENDARALALGE 199 Query: 170 WQYGAARGYKNVVXXXXXXXXXXXIVSDGKVVYGTTFSAGEIGHMNMFXXXXXXXXXXXX 229 +G +G +V+ IV + ++ +G AGEIGH + Sbjct: 200 TWFGNGQGIDHVICVNVGVGIGAGIVLNKRLFHGVHGIAGEIGHTTVDIHGKKCTCGNYG 259 Query: 230 XXXXYVSAVGMVNTFTEKLAEGRPSIIQAWTNGNNGQITAQMISEAYDLEDPLAAEVMHE 289 + +A GR +++ G+ +I+ ++I + DPL+ E++ E Sbjct: 260 CLQTVAGGNMLKERAIHDIASGRKTMLLEKVEGDMDRISGEIIHQCALAGDPLSIEILEE 319 Query: 290 TGVLLGFGLANVINLLNPEVIVVGGGMSXXXXAGERLLHSVRETVKDHALKLSGSKCSIV 349 G LG G+ N+IN +NP+ I+VGGG+S AG+ +L +R+ VK+ AL + +V Sbjct: 320 AGKYLGIGITNIINFINPDRIIVGGGVS---KAGDFVLEPLRKMVKERALTAKARETEVV 376 Query: 350 QAELGSRAGTLGA 362 AELG +GA Sbjct: 377 TAELGEWGTAIGA 389 >SP0668 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 320 Score = 120 bits (301), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 94/321 (29%), Positives = 155/321 (48%), Gaps = 12/321 (3%) Query: 48 MKTYTIGVDLGGTNIKAALFDASFNAIGEISLSTEAAKGPAHVLSRIRLAVQQLTEDKGI 107 M IG+DLGGT+IK A+ + G+ S+ T +H++ + ++Q + G+ Sbjct: 2 MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIVDDMIESIQHRLDLLGL 61 Query: 108 PLQSITCMGLGIPGLLDPEEGMSLFSPNFPGWEHIH-IVNEMKLYYDFPIFIDNDVRVNL 166 +G+G PG++D ++G + + N W+ + I +++ P FIDND V Sbjct: 62 AAADFQGIGMGSPGVVDRDKGTVIGAYNLN-WKTLQPIKQKIEKALGIPFFIDNDANVAA 120 Query: 167 YGEWQYGAARGYKNVVXXXXXXXXXXXIVSDGKVVYGTTFSAGEIGHMNM-FXXXXXXXX 225 GE GA +VV IV++GK+++G +AGE+GH+ + F Sbjct: 121 LGERWMGAGDNQPDVVFITLGTGVGGGIVAEGKLLHGVAGAAGELGHITVDFDQPISCTC 180 Query: 226 XXXXXXXXYVSAVGMVNTFTEKLA---EGRPSIIQAWTNGNNGQITAQMISEAYDLEDPL 282 SA G+VN T + A EG ++ + NG ++TA+ + + D L Sbjct: 181 GKKGCLETVASATGIVN-LTRRYADEYEGDAALKRLIDNGE--EVTAKTVFDLAKEGDDL 237 Query: 283 AAEVMHETGVLLGFGLANVINLLNPEVIVVGGGMSXXXXAGERLLHSVRETVKDHALKLS 342 A V LG AN+ ++LNP IV+GGG+S AGE LL V++ +++ Sbjct: 238 ALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVS---AAGEFLLQGVQKVYDENSFPQV 294 Query: 343 GSKCSIVQAELGSRAGTLGAA 363 + + A LG+ AG +GAA Sbjct: 295 RTSTKLALATLGNDAGVIGAA 315 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,669,609 Number of extensions: 936921 Number of successful extensions: 1936 Number of sequences better than 1.0e-05: 68 Number of HSP's gapped: 1847 Number of HSP's successfully gapped: 69 Length of query: 375 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 267 Effective length of database: 25,922,359 Effective search space: 6921269853 Effective search space used: 6921269853 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)