BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0020.p (260 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMB1254 [H] COG0807 GTP cyclohydrolase II 161 1e-39 NMA1425 [H] COG0807 GTP cyclohydrolase II 160 3e-39 PA4047 [H] COG0807 GTP cyclohydrolase II 154 2e-37 MT1458_2 [H] COG0807 GTP cyclohydrolase II 150 2e-36 Rv1415_2 [H] COG0807 GTP cyclohydrolase II 150 2e-36 >NMB1254 [H] COG0807 GTP cyclohydrolase II Length = 197 Score = 161 bits (407), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 80/168 (47%), Positives = 112/168 (66%), Gaps = 1/168 (0%) Query: 78 AEGQQSSVLVYGDFADSDEALIRMHSICHTGDIFGSKRCDCGFQLRQSMKMIAQHGSGAL 137 A GQ+ L G+F+D + L R+HS C TGD S++CDCG QL +M+ + G G + Sbjct: 28 ANGQEHVALTVGNFSDGNPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGII 87 Query: 138 FYLANHEGRGIGLFSKAMAYILQEEGYDTVEANLELGFADDSRDYRDAISVLQHLRSKPV 197 YL EGRGIGL +K AY LQ++G DTVEANL LG D+RD+R A S+ ++L + V Sbjct: 88 VYL-RQEGRGIGLINKIRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSV 146 Query: 198 TLITNNPKKMEALKAAGMNTGKRIPLWGDVSVFNEKYLRTKVSRSGHL 245 L+TNNP+K++ LK AG+N +RIPL ++ N++YL+TK + GHL Sbjct: 147 KLLTNNPEKIQTLKDAGINVVERIPLHVGENLENKRYLQTKADKLGHL 194 >NMA1425 [H] COG0807 GTP cyclohydrolase II Length = 477 Score = 160 bits (404), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 13/181 (7%), Positives = 35/181 (19%), Gaps = 1/181 (0%) Query: 65 TELLISRLSSMNLAEGQQSSVLVYGDFADSDEALIRMHSICHTGDIFGSKRCDCGFQLRQ 124 E++ +S + + ++ + + + + Sbjct: 15 PEVITYIVSLESQIKELTERLIALDSRLNQNSRNSSRPPSTDYFVKEKPNPKSLRKKSGK 74 Query: 125 SMKMIAQHGSGALFYLANHEGRGIGLFSKAMAYILQEEGYDTVEANLELGFADDSRDYRD 184 H L + + I VEA + D Sbjct: 75 KPGGQDGHPGTTLEMV-DEPEXEIEHSLSCCKECGHTLENVEVEAYEKRQVFDIPPVNLI 133 Query: 185 AISVLQHLRSKPVTLITNNPKKMEALKAAGMNTGKRIPLWGDVSVFNEKYLRTKVSRSGH 244 +++ P N E++K + + Sbjct: 134 VTEHKSQIKTCPHCGRLNKAAFPESVKYPVQYGPNILASAVYCKNHHFIPYERISEFFED 193 Query: 245 L 245 + Sbjct: 194 I 194 >PA4047 [H] COG0807 GTP cyclohydrolase II Length = 205 Score = 154 bits (388), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 78/166 (46%), Positives = 103/166 (62%), Gaps = 1/166 (0%) Query: 80 GQQSSVLVYGDFADSDEALIRMHSICHTGDIFGSKRCDCGFQLRQSMKMIAQHGSGALFY 139 G++ L GD AD L R+HS C TGD S RCDCGFQL ++ IA+ G G L Y Sbjct: 29 GKEHVALSMGDIADGAPVLGRLHSECLTGDALFSLRCDCGFQLEGALAAIAEEGRGVLLY 88 Query: 140 LANHEGRGIGLFSKAMAYILQEEGYDTVEANLELGFADDSRDYRDAISVLQHLRSKPVTL 199 L EGRGIGL +K AY LQ+ G DTVEANL+LGF D RDY +L HL + L Sbjct: 89 L-RQEGRGIGLLNKIRAYELQDGGADTVEANLQLGFGADQRDYAMCQPMLAHLGVSSLRL 147 Query: 200 ITNNPKKMEALKAAGMNTGKRIPLWGDVSVFNEKYLRTKVSRSGHL 245 +TNNP+K++AL++ + +R+PL ++ N +YL TK + GH+ Sbjct: 148 MTNNPRKVKALESYAITVAERVPLQKGLNKHNRRYLATKAGKLGHM 193 >MT1458_2 [H] COG0807 GTP cyclohydrolase II Length = 220 Score = 150 bits (379), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 78/169 (46%), Positives = 105/169 (62%), Gaps = 1/169 (0%) Query: 87 VYGDFADSDEALIRMHSICHTGDIFGSKRCDCGFQLRQSMKMIAQHGSGALFYLANHEGR 146 + G AD D+ L+R+HS C TGD+FGS+RCDCG QL ++ M+A+ G G + Y+ HEGR Sbjct: 41 IAGPNADGDDVLVRVHSECLTGDVFGSRRCDCGPQLDAALAMVAREGRGVVLYMRGHEGR 100 Query: 147 GIGLFSKAMAYILQEEGYDTVEANLELGFADDSRDYRDAISVLQHLRSKPVTLITNNPKK 206 GIGL K AY LQ+ G DTV+ANL+LG D+RDY +L L + + L+TNNP K Sbjct: 101 GIGLMHKLQAYQLQDAGADTVDANLKLGLPADARDYGIGAQILVDLGVRSMRLLTNNPAK 160 Query: 207 MEALKAAGMNTGKRIPLWGDVSVFNEKYLRTKVSRSGHLEAVPDADFFE 255 L G++ +R+PL + N +YL TK + GH A D DF E Sbjct: 161 RVGLDGYGLHIIERVPLPVRANAENIRYLMTKRDKLGHDLAGLD-DFHE 208 >Rv1415_2 [H] COG0807 GTP cyclohydrolase II Length = 220 Score = 150 bits (379), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 78/169 (46%), Positives = 105/169 (62%), Gaps = 1/169 (0%) Query: 87 VYGDFADSDEALIRMHSICHTGDIFGSKRCDCGFQLRQSMKMIAQHGSGALFYLANHEGR 146 + G AD D+ L+R+HS C TGD+FGS+RCDCG QL ++ M+A+ G G + Y+ HEGR Sbjct: 41 IAGPNADGDDVLVRVHSECLTGDVFGSRRCDCGPQLDAALAMVAREGRGVVLYMRGHEGR 100 Query: 147 GIGLFSKAMAYILQEEGYDTVEANLELGFADDSRDYRDAISVLQHLRSKPVTLITNNPKK 206 GIGL K AY LQ+ G DTV+ANL+LG D+RDY +L L + + L+TNNP K Sbjct: 101 GIGLMHKLQAYQLQDAGADTVDANLKLGLPADARDYGIGAQILVDLGVRSMRLLTNNPAK 160 Query: 207 MEALKAAGMNTGKRIPLWGDVSVFNEKYLRTKVSRSGHLEAVPDADFFE 255 L G++ +R+PL + N +YL TK + GH A D DF E Sbjct: 161 RVGLDGYGLHIIERVPLPVRANAENIRYLMTKRDKLGHDLAGLD-DFHE 208 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,497,590 Number of extensions: 711614 Number of successful extensions: 1300 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1290 Number of HSP's successfully gapped: 11 Length of query: 260 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 156 Effective length of database: 26,383,695 Effective search space: 4115856420 Effective search space used: 4115856420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)