BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0011.p (486 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1353_1 [G] COG1263 Phosphotransferase system IIC components, ... 359 5e-99 STM0685_1 [G] COG1263 Phosphotransferase system IIC components, ... 342 1e-93 BH0422_1 [G] COG1263 Phosphotransferase system IIC components, g... 338 9e-93 SA0183_1 [G] COG1263 Phosphotransferase system IIC components, g... 333 3e-91 BH0844_1 [G] COG1263 Phosphotransferase system IIC components, g... 332 6e-91 >CAC1353_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 409 Score = 359 bits (922), Expect = 5e-99, Method: Compositional matrix adjust. Identities = 198/390 (50%), Positives = 263/390 (67%), Gaps = 26/390 (6%) Query: 2 NGMLAFLQKLGKSLMLPVATLPAAAILQGFALINYEKDFHLGAIGRFINTYIAPFLNAGA 61 N +LA QKLGKSLM P+A LPAA +L LG +N I+ + AG Sbjct: 5 NKLLAACQKLGKSLMTPIAVLPAAGLL-----------LRLGQ-PDLLN--ISWMMAAGN 50 Query: 62 GAIFGNLALIFAIGVAIGFA--GDAVAALSALIAYQILDSILKVIPAQMPFIGDDVKLNM 119 G IF NLA+IFAIG+A+GFA + VA LSA + Y +L ++ + ++M Sbjct: 51 G-IFNNLAMIFAIGIAVGFAEENNGVAGLSAAVGYFVLTNVATSF---------NKHIDM 100 Query: 120 GVLGGIFVGAWAAFLYKKYHNIKMPDWLGFFAGKRFVPIITAVTTIVLSVLVGMIWSPIQ 179 GVLGGI VG A LY KY + ++PD+LGFF G+R VPI+T++ ++VL ++ G++W IQ Sbjct: 101 GVLGGIIVGILAGNLYNKYKSTRLPDFLGFFGGRRLVPILTSLCSLVLGLISGLVWPAIQ 160 Query: 180 DVISDFGTWVVGLGGVGAFIFGTANRLLIPFGLHHVINAIAWFQIGDFTNAAGEVVHGDL 239 +VI+ FG V G VG+FI+G NRLLIP GLHHV+N + WFQ G F +A+G++V GDL Sbjct: 161 NVINAFGNSVSHAGVVGSFIYGLLNRLLIPIGLHHVLNTLFWFQFGTFKSASGKIVTGDL 220 Query: 240 WRFFAGDKSAGMFMTGFFPIMMFALPGAALAFIHTAKPEKRKMVASIFIGSAIASFLTGI 299 RFFA DK+AG +MTGFFPIMMFALP A LA I AK E RK V+ + +G A +FLTG+ Sbjct: 221 HRFFALDKTAGTYMTGFFPIMMFALPAACLAMISAAKKENRKKVSGMLLGIAFTAFLTGV 280 Query: 300 TEPLEFSFMFIAPVLYLVHALLTGFAGFLMYVLDVKLGFGFSAGLIDYLLNMKLSTNAWV 359 TEP+EF FMF+APVLY+VHA+LTG + + L +K GF FSAG +DY++N +ST + Sbjct: 281 TEPIEFLFMFLAPVLYVVHAVLTGLSMAITSALGIKSGFTFSAGFVDYIMNFNISTKPIL 340 Query: 360 LIPVGLGFFVLYYVLFRFIITKFNLKTPGR 389 LI +G+ + ++YY LF F I KFNL TPGR Sbjct: 341 LIVIGILYAIIYYFLFLFTIKKFNLPTPGR 370 >STM0685_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 388 Score = 342 bits (876), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 191/391 (48%), Positives = 254/391 (64%), Gaps = 29/391 (7%) Query: 4 MLAFLQKLGKSLMLPVATLPAAAILQGFALINYEKDFHLGAIGRFINTYIAPFLNAGAGA 63 +L F Q+LG++L LP+A LP AA+L F + D +N PF+ G+ Sbjct: 3 ILGFFQRLGRALQLPIAVLPVAALLLRFG----QPDL--------LNM---PFIAQAGGS 47 Query: 64 IFGNLALIFAIGVAIGFAGDAVAALSALIA--YQILDSILKVIPAQMPFIGDDVKLNMGV 121 IF NLAL+FAIGVA ++ D+ A + A Y ++ + I + ++NMGV Sbjct: 48 IFDNLALVFAIGVASSWSKDSAGAAALAGAVGYFVMTKAMVTI---------NPEINMGV 98 Query: 122 LGGIFVGAWAAFLYKKYHNIKMPDWLGFFAGKRFVPIITAVTTIVLSVLVGMIWSPIQDV 181 L GI G +Y ++ IK+PD+L FF GKRFVPI T +VL+ + G +W P+Q Sbjct: 99 LAGIITGLVGGAVYNRWSGIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHG 158 Query: 182 ISDFGTWVVGLGGVGAFIFGTANRLLIPFGLHHVINAIAWFQIGDFTNAAGEVVHGDLWR 241 I G W+V G +G+ IFG NRLLIP GLH V+N IAWFQIG+FTNAAG V HGD+ R Sbjct: 159 IHAGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR 218 Query: 242 FFAGDKSAGMFMTGFFPIMMFALPGAALAFIHTAKPEKRKMVASIFIGSAIASFLTGITE 301 F+AGD +AGMFM+GFFPIMMF LPGAALA A E+R MV + + AI +FLTG+TE Sbjct: 219 FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAITAFLTGVTE 278 Query: 302 PLEFSFMFIAPVLYLVHALLTGFAGFLMYVLDVKLGFGFSAGLIDYLLNMKL---STNAW 358 PLEF FMF+AP+LYL+HA+LTG + F+ +L + GF FSAG IDY+L L S N W Sbjct: 279 PLEFLFMFLAPLLYLLHAILTGISLFVATLLGIHAGFSFSAGAIDYVLMYNLPAASNNVW 338 Query: 359 VLIPVGLGFFVLYYVLFRFIITKFNLKTPGR 389 +L+ +G+ FF++Y++LF +I FNLKTPGR Sbjct: 339 MLLVMGVVFFIIYFLLFSAVIRMFNLKTPGR 369 >BH0422_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 375 Score = 338 bits (868), Expect = 9e-93, Method: Compositional matrix adjust. Identities = 195/388 (50%), Positives = 259/388 (66%), Gaps = 31/388 (7%) Query: 4 MLAFLQKLGKSLMLPVATLPAAAILQGFALINYEKDFHLGAIGRFINTYIAPFLNAGAGA 63 ML FLQ++GKSLMLP+A LPAAAIL LG F +IA A A Sbjct: 1 MLTFLQRIGKSLMLPIAVLPAAAIL-----------LRLGQEDLFNQAFIA----AAGAA 45 Query: 64 IFGNLALIFAIGVAIGFAGDA--VAALSALIAYQILDSILKVIPAQMPFIGDDVKLNMGV 121 +F +L LIFAIGVAIGF+ D+ A L+ I Y +L + + I D ++M + Sbjct: 46 VFDHLPLIFAIGVAIGFSKDSNGTAGLAGAIGYLVLVNGTQAI---------DPNVDMAI 96 Query: 122 LGGIFVGAWAAFLYKKYHNIKMPDWLGFFAGKRFVPIITAVTTIVLSVLVGMIWSPIQDV 181 LGGI G A LY ++H++K+P WLGFF G+RFVPIITA ++ + L G +W PIQD Sbjct: 97 LGGIISGIVAGLLYNRFHDVKLPQWLGFFGGRRFVPIITATVMVIFAGLFGYVWPPIQDG 156 Query: 182 ISDFGTWVVGLGGVGAFIFGTANRLLIPFGLHHVINAIAWFQIGDFTNAAGEVVHGDLWR 241 I+ G W++G G +G IFG NRLLIP GLHHV+N++ WF G++ A G DL R Sbjct: 157 INQIGQWIIGGGALGVGIFGFLNRLLIPVGLHHVLNSLVWFVFGEYEGATG-----DLHR 211 Query: 242 FFAGDKSAGMFMTGFFPIMMFALPGAALAFIHTAKPEKRKMVASIFIGSAIASFLTGITE 301 FFAGD +AG FM GFFPIMMF LP AALA I A+PE+RK V + IG A+ +F+TG+TE Sbjct: 212 FFAGDPTAGSFMAGFFPIMMFGLPAAALAMIAAAEPERRKEVTGMLIGIAVTAFVTGVTE 271 Query: 302 PLEFSFMFIAPVLYLVHALLTGFAGFLMYVLDVKLGFGFSAGLIDYLLNMKLSTNAWVLI 361 PLEFSFMF+AP+LY+ HALLT + + ++L+++ GFGFS G ID++LN +L+T +LI Sbjct: 272 PLEFSFMFLAPILYVAHALLTASSLAIAHLLEIRHGFGFSGGAIDFVLNWRLATKPALLI 331 Query: 362 PVGLGFFVLYYVLFRFIITKFNLKTPGR 389 +GL + ++Y+VLF +I KF+LKTPGR Sbjct: 332 VMGLIYALIYFVLFYVVIKKFDLKTPGR 359 >SA0183_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 428 Score = 333 bits (855), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 194/409 (47%), Positives = 260/409 (63%), Gaps = 32/409 (7%) Query: 8 LQKLGKSLMLPVATLPAAAIL-------QGFALINYEKDFHLGAIGRFINTYIAPFLNAG 60 LQ++GK+LMLPVA LPAA +L QG AL +Y G + +A + A Sbjct: 9 LQRIGKALMLPVAILPAAGLLLAIGTAIQGEALQHYLPFIQNGGVQN-----VAKLMTAA 63 Query: 61 AGAIFGNLALIFAIGVAIGFAG-DAVAALSALIAYQILDSI----LKVIPAQM--PFIGD 113 IF NL +IFA+GVAIG AG D VAA++A + Y I++ L+V P + P G Sbjct: 64 GSIIFENLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMNKTMGDFLQVTPKNVTDPASGY 123 Query: 114 DV-----KLNMGVLGGIFVGAWAAFLYKKYHNIKMPDWLGFFAGKRFVPIITAVTTIVLS 168 L GV GGI +GA AA+ Y K++NI +P +LGFFAGKRFVPI+ A T+ +L+ Sbjct: 124 ASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPSYLGFFAGKRFVPIMMATTSFILA 183 Query: 169 VLVGMIWSPIQDVISDFGTWVVGLG-GVGAFIFGTANRLLIPFGLHHVINAIAWFQIGDF 227 + +IW IQ ++ F T ++ GV F+FG RLLIPFGLHH+ +A WF+ G + Sbjct: 184 FPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGFIKRLLIPFGLHHIFHAPFWFEFGSW 243 Query: 228 TNAAGEVVHGDLWRFFAGDK-----SAGMFMTGFFPIMMFALPGAALAFIHTAKPEKRKM 282 NAAGE++HGD F + +AG FM G FP+MMF LP AALA HTAKPE +K+ Sbjct: 244 KNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQGEFPVMMFGLPAAALAIYHTAKPENKKV 303 Query: 283 VASIFIGSAIASFLTGITEPLEFSFMFIAPVLYLVHALLTGFAGFLMYVLDVKLGFGFSA 342 VA + +A+ SFLTGITEPLEFSF+F+AP+L+ +HA+L G + +Y+LDV LG+ FS Sbjct: 304 VAGLMGSAALTSFLTGITEPLEFSFLFVAPLLFFIHAVLDGLSFLTLYLLDVHLGYTFSG 363 Query: 343 GLIDYLL--NMKLSTNAWVLIPVGLGFFVLYYVLFRFIITKFNLKTPGR 389 G IDY+L + T W++IPVGL + V+YY +FRF+I K KTPGR Sbjct: 364 GFIDYVLLGVLPNKTQWWLVIPVGLVYAVIYYFVFRFLIVKLKYKTPGR 412 >BH0844_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 424 Score = 332 bits (852), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 194/403 (48%), Positives = 254/403 (63%), Gaps = 21/403 (5%) Query: 8 LQKLGKSLMLPVATLPAAAILQGF--ALINYEKDFHLGAIGRFINTYIAPFLNAGAGAIF 65 LQ++GK+LMLPVA LPAA IL F AL N + + IA + +F Sbjct: 9 LQRVGKALMLPVALLPAAGILLAFGDALQNPDMTERFPFLTADWIVIIAELMQNAGDIVF 68 Query: 66 GNLALIFAIGVAIGFA-GDAVAALSALIAYQILDSILKVIPAQMP-FIGDDV-------- 115 NLAL+FA+GVAIG + GD VA L+ALI Y I++ + VI P +GD Sbjct: 69 ANLALLFAVGVAIGLSNGDGVAGLAALIGYLIINVTMSVIGHVTPEMVGDSPAYASVLGI 128 Query: 116 -KLNMGVLGGIFVGAWAAFLYKKYHNIKMPDWLGFFAGKRFVPIITAVTTIVLSVLVGMI 174 L GV GGI G A++YKKY NI++P +LGFFAGKRFVPI TA ++ + VL+ I Sbjct: 129 PTLQTGVFGGIIAGLLGAYMYKKYFNIELPSYLGFFAGKRFVPIATAFFSLFVGVLLHFI 188 Query: 175 WSPIQDVISDFGTWVVGLG-GVGAFIFGTANRLLIPFGLHHVINAIAWFQIGDFTNAAGE 233 W +QD ++ F ++ V AFIFG R LIPFGLHH+ + WF+ G +TNAAGE Sbjct: 189 WPTVQDGLNAFSYYMTESNRTVAAFIFGVIERALIPFGLHHIFYSPFWFEFGQYTNAAGE 248 Query: 234 VVHGDLWRFFA-----GDKSAGMFMTGFFPIMMFALPGAALAFIHTAKPEKRKMVASIFI 288 +V GD FFA + +AG FM G FP MMF LP AALA H A+PE +K+VA I Sbjct: 249 IVRGDQRIFFAQIRDGAELTAGTFMAGKFPFMMFGLPAAALAIYHCARPENKKVVAGIMA 308 Query: 289 GSAIASFLTGITEPLEFSFMFIAPVLYLVHALLTGFAGFLMYVLDVKLGFGFSAGLIDYL 348 +A+ SFLTGITEP+EFSF+F+AP+L+ VH + G + +M++LD+K+G FS G+ID++ Sbjct: 309 SAALTSFLTGITEPIEFSFLFVAPLLFAVHTIFAGLSFMIMHILDIKIGMTFSGGVIDFI 368 Query: 349 LNMKL--STNAWVLIPVGLGFFVLYYVLFRFIITKFNLKTPGR 389 L L T W++IP+GL F V+YY FRF I KFNL TPGR Sbjct: 369 LYGVLPNRTAWWLVIPIGLVFSVIYYFGFRFAIKKFNLMTPGR 411 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.145 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,098,452 Number of extensions: 1482000 Number of successful extensions: 4618 Number of sequences better than 1.0e-05: 96 Number of HSP's gapped: 4433 Number of HSP's successfully gapped: 103 Length of query: 486 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 376 Effective length of database: 25,691,691 Effective search space: 9660075816 Effective search space used: 9660075816 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 118 (50.1 bits)