BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0010.p (171 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP0577_3 [G] COG2190 Phosphotransferase system IIA components 135 4e-32 BH0296_3 [G] COG2190 Phosphotransferase system IIA components 129 2e-30 CAC1407_3 [G] COG2190 Phosphotransferase system IIA components 126 1e-29 Zcrr [G] COG2190 Phosphotransferase system IIA components 126 2e-29 ECs3289 [G] COG2190 Phosphotransferase system IIA components 126 2e-29 >SP0577_3 [G] COG2190 Phosphotransferase system IIA components Length = 166 Score = 135 bits (339), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 61/124 (49%), Positives = 87/124 (70%) Query: 22 ILAPVSGQAVSLTGVPDETFSGGHMGQGIAIEPSEGKLIAPFDGKIAHVVKSSHAVIIEH 81 I +P+SG+ + L+ V D+TFS +G G+AI PSEGK+ APFDGKI ++ + HA+ ++ Sbjct: 20 IFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFDGKITNIFPTKHAIGLKS 79 Query: 82 SSGLQLLLHIGMDTVSLKGSGFISHIASGDEVKAGQTLIEFDIASIQAAGYQTITPVIVT 141 G++LL+HIG+DTV LKG GFISH+ GD V Q + E D+ I+ GY+T+TPVIVT Sbjct: 80 DEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEMDLNLIKTKGYETVTPVIVT 139 Query: 142 RNGE 145 + Sbjct: 140 NTND 143 >BH0296_3 [G] COG2190 Phosphotransferase system IIA components Length = 161 Score = 129 bits (324), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 62/138 (44%), Positives = 88/138 (63%), Gaps = 4/138 (2%) Query: 5 KWKSKKEAQPSKITTLEILAPVSGQAVSLTGVPDETFSGGHMGQGIAIEPSEGKLIAPFD 64 + KKE S+ EI +P+ G+ V LT V D FS G MG+G+A+ P EG+L+AP + Sbjct: 2 REDDKKEPSASE----EIKSPLKGEVVPLTEVQDHVFSSGAMGKGVAVRPKEGRLVAPIN 57 Query: 65 GKIAHVVKSSHAVIIEHSSGLQLLLHIGMDTVSLKGSGFISHIASGDEVKAGQTLIEFDI 124 G + + ++ HA+ I +G ++ +H+G+DTV LKG F S I GDEV AG L+EFD+ Sbjct: 58 GTVTSLFETKHAIGITSDNGTEIFIHVGIDTVQLKGEHFTSFIEQGDEVAAGDVLLEFDV 117 Query: 125 ASIQAAGYQTITPVIVTR 142 I AAGY ITPV++T Sbjct: 118 ERITAAGYDVITPVLITN 135 >CAC1407_3 [G] COG2190 Phosphotransferase system IIA components Length = 155 Score = 126 bits (317), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 67/146 (45%), Positives = 96/146 (65%), Gaps = 1/146 (0%) Query: 22 ILAPVSGQAVSLTGVPDETFSGGHMGQGIAIEPSEGKLIAPFDGKIAHVVKSSHAVIIEH 81 +++P+ G+ +L+ V DE FS G +G+GIAIEP EGKL++P DG +A + + HAV I Sbjct: 9 LVSPLKGKIKTLSEVKDEAFSTGSLGKGIAIEPEEGKLVSPVDGVLATLFPTGHAVGIIS 68 Query: 82 SSGLQLLLHIGMDTVSLKGSGFISHIASGDEVKAGQTLIEFDIASIQAAGYQTITPVIVT 141 G ++L+H+GMDTV L+G F + + GD VKAG T++EFDI I+ AGY TPV+VT Sbjct: 69 DKGTEILIHVGMDTVQLEGKYFKTILKQGDHVKAGDTILEFDIPKIKKAGYTLTTPVVVT 128 Query: 142 RNGEADPEVVCRYGSVTAGKDIILTV 167 N E+ +V+ KD +LTV Sbjct: 129 -NSESYLDVIETDKIKVERKDQLLTV 153 >Zcrr [G] COG2190 Phosphotransferase system IIA components Length = 169 Score = 126 bits (317), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 65/160 (40%), Positives = 97/160 (60%), Gaps = 1/160 (0%) Query: 2 MFSKWKSKKEAQPSKITTLEILAPVSGQAVSLTGVPDETFSGGHMGQGIAIEPSEGKLIA 61 +F K KS T+EI+AP+SG+ V++ VPD F+ +G GIAI+P+ K++A Sbjct: 3 LFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPTGNKMVA 62 Query: 62 PFDGKIAHVVKSSHAVIIEHSSGLQLLLHIGMDTVSLKGSGFISHIASGDEVKAGQTLIE 121 P DG I + +++HA IE SG++L +H G+DTV LKG GF G VK G T+IE Sbjct: 63 PVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGEGFKRIAEEGQRVKVGDTVIE 122 Query: 122 FDIASIQAAGYQTITPVIVTRNGEADPEVVCRYGSVTAGK 161 FD+ ++ T+TPV+++ E E++ GSVT G+ Sbjct: 123 FDLPQLEEKAKSTLTPVVISNMDEI-KELIKLSGSVTVGE 161 >ECs3289 [G] COG2190 Phosphotransferase system IIA components Length = 169 Score = 126 bits (317), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 65/160 (40%), Positives = 97/160 (60%), Gaps = 1/160 (0%) Query: 2 MFSKWKSKKEAQPSKITTLEILAPVSGQAVSLTGVPDETFSGGHMGQGIAIEPSEGKLIA 61 +F K KS T+EI+AP+SG+ V++ VPD F+ +G GIAI+P+ K++A Sbjct: 3 LFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPTGNKMVA 62 Query: 62 PFDGKIAHVVKSSHAVIIEHSSGLQLLLHIGMDTVSLKGSGFISHIASGDEVKAGQTLIE 121 P DG I + +++HA IE SG++L +H G+DTV LKG GF G VK G T+IE Sbjct: 63 PVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGEGFKRIAEEGQRVKVGDTVIE 122 Query: 122 FDIASIQAAGYQTITPVIVTRNGEADPEVVCRYGSVTAGK 161 FD+ ++ T+TPV+++ E E++ GSVT G+ Sbjct: 123 FDLPQLEEKAKSTLTPVVISNMDEI-KELIKLSGSVTVGE 161 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.132 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,576,113 Number of extensions: 493711 Number of successful extensions: 1933 Number of sequences better than 1.0e-05: 49 Number of HSP's gapped: 1928 Number of HSP's successfully gapped: 49 Length of query: 171 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 72 Effective length of database: 26,960,365 Effective search space: 1941146280 Effective search space used: 1941146280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 112 (47.8 bits)