BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0518.0006.p (274 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1593 [G] COG0406 Fructose-2,6-bisphosphatase 87 3e-17 DR0278 [G] COG0406 Fructose-2,6-bisphosphatase 82 7e-16 CAC1385 [G] COG0406 Fructose-2,6-bisphosphatase 79 8e-15 RSc0499 [G] COG0406 Fructose-2,6-bisphosphatase 73 4e-13 CAC3021 [G] COG0406 Fructose-2,6-bisphosphatase 72 8e-13 >BH1593 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 86.7 bits (213), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 13/169 (7%), Positives = 26/169 (15%), Gaps = 17/169 (10%) Query: 75 LELVLIRHGHTLWNKERRYLGSTDLALLPESRXXXXXXXQ--PELAGSFWRVYCSDMRRC 132 TL + A ++A +Y R Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVA----EMYPVPPRPE 60 Query: 133 RETLELIAPALLQNAIYDSRLREMSFGAWEGHTYEQLQYLPQYRSWIDDPAASTPPGGEA 192 + L ++ + + +D Sbjct: 61 TQGLTETYVGNWLAKH--GSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSL 118 Query: 193 WSEFTGRVEHFVSGLLQAAAAVPGLKNSSHAGPLRVLVVTHGGIIRQLL 241 T ++ + Q T LL Sbjct: 119 KRLQTDYLDLYQVHWPQRPTN-----CFGK----LGYSWTDSAPAVSLL 158 >DR0278 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 82.4 bits (202), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 9/163 (5%), Positives = 32/163 (19%), Gaps = 9/163 (5%) Query: 71 SGLVLELVLIRHGHTLWNKERRYLGSTDLALLPESRXXXXXXXQPELAG-SFWRVYCSDM 129 + + +++ + + + V Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGIN-LIDVAEMYPVPPRPE 60 Query: 130 RRCRETLELIAPALLQNAIYDSRLREMSFGAWEGHTYEQLQYLPQYRSWIDDPAASTPPG 189 + + +L S + ++ Q + A Sbjct: 61 TQGLTETYVGNWL--AKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSL 118 Query: 190 GEAWSEFTGRVEHFVSGLLQAAAAVPG--LKNSSHAGPLRVLV 230 +++ + Q G + + + P L+ Sbjct: 119 KRLQTDYLDLYQVH---WPQRPTNCFGKLGYSWTDSAPAVSLL 158 >CAC1385 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 79.0 bits (193), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 5/175 (2%), Positives = 22/175 (12%), Gaps = 19/175 (10%) Query: 74 VLELVLIRHGHTLWNKERRYLGSTDLALLPESRXXXXXXXQPELAGSFWRVYCSDMRRCR 133 + + + + + ++ + R Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 134 ETLELIAPALLQNAIYDSRLREMSFGAWEGHTYEQL--QYLPQYRSWIDDPAASTPPGGE 191 + + +D Sbjct: 61 TQGLTETYV---GNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDS 117 Query: 192 AWSEFTGRVEHFVSGLLQAAAAVPGLKNSSHAGPLRVLVVTHGGIIRQLLARTME 246 T ++ + P + A ++ Sbjct: 118 LKRLQTDYLDLYQVHW-------PQRPTN-------CFGKLGYSWTDSAPAVSLL 158 >RSc0499 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 73.2 bits (178), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 3/167 (1%), Positives = 22/167 (13%), Gaps = 15/167 (8%) Query: 74 VLELVLIRHGHTLWNKERRYLGSTDLALLPESRXXXXXXXQPELAGSFWRVYCSDMRRCR 133 + ++ + + + + + Sbjct: 14 STLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWL 73 Query: 134 ETLELIAPALLQNAIYDSRLREMSFGAWEGHTYEQL--QYLPQYRSWIDD-PAASTPPGG 190 ++ + + + + + L + Sbjct: 74 AKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHW 133 Query: 191 EAWSEFTGRVEHFVSGLLQAAAAVPGLKNSSHAGPLRVLVVTHGGII 237 + +A L + Sbjct: 134 PQRPTNCFGK---LGYSWTDSAPAVS---------LLDTLDALAEYQ 168 >CAC3021 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 72.4 bits (176), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 11/168 (6%), Positives = 26/168 (15%), Gaps = 16/168 (9%) Query: 77 LVLIRHGHTLWNKERRYLGSTDLALLPESRXXXXXXXQPELAGSFWRVYCSDMRRCRETL 136 + + ++ + R Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSE-ADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 137 ELIAPALLQNAIYDSRLREMSFGAWEGHTYEQL--QYLPQYRSWIDDPAASTPPGGE-AW 193 A + SR + + G + Q + A Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 194 SEFTGRVEHFVSGLLQAAAAVPGLKNSSHAGPLRVLVVTHGGIIRQLL 241 H+ + ++ G L + L Sbjct: 124 DYLDLYQVHWP------------QRPTNCFGKLGYSWTDSAPAVSLLD 159 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,405,352 Number of extensions: 766385 Number of successful extensions: 1250 Number of sequences better than 1.0e-05: 51 Number of HSP's gapped: 1215 Number of HSP's successfully gapped: 52 Length of query: 274 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 169 Effective length of database: 26,268,361 Effective search space: 4439353009 Effective search space used: 4439353009 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)