BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0518.0004.p (333 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZddlB [M] COG1181 D-alanine-D-alanine ligase and related ATP-gra... 199 4e-51 ddlB [M] COG1181 D-alanine-D-alanine ligase and related ATP-gras... 199 4e-51 ZddlB [M] COG1181 D-alanine-D-alanine ligase and related ATP-gra... 199 4e-51 ECs0096 [M] COG1181 D-alanine-D-alanine ligase and related ATP-g... 199 4e-51 STM0130 [M] COG1181 D-alanine-D-alanine ligase and related ATP-g... 197 2e-50 >ZddlB [M] COG1181 D-alanine-D-alanine ligase and related ATP-grasp enzymes Length = 306 Score = 199 bits (507), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 127/305 (41%), Positives = 176/305 (57%), Gaps = 19/305 (6%) Query: 3 KVGVIMGGVSSEYEVSLNTGREMLKHLDRSKYEGVPVVIHSREELVDAVKGLDF--ALLA 60 K+ V++GG S+E EVSLN+G +L L + PV +E V +K + F +A Sbjct: 4 KIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPV--DPKEVDVTQLKSMGFQKVFIA 61 Query: 61 LHGAYGEDGTVQGTLETLGIPYSGSGVLASSLCMDKHLSKTIIRSKGVHTPDWLCLDRME 120 LHG GEDGT+QG LE +G+PY+GSGV+AS+L MDK SK + + G+ W+ L R+E Sbjct: 62 LHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKLLWQGAGLPVAPWVALTRVE 121 Query: 121 ------DLSPEAVEALGYPVMVKPNSGGSSIGMTKVNSSAELRSAVEKAFAADCSVLIEE 174 D + ALG PV+VKP+ GSS+GM+KV + L+ A+ AF D VLIE+ Sbjct: 122 FEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAENALQDALRLAFQHDEEVLIEK 181 Query: 175 YTEGREITCPILGGTLLPVIGIRSLGADWFDYSAKYELGGAEERVIQLPAEL----EERV 230 + G E T ILG +LP + I+ G ++DY AKY ++E PA L E + Sbjct: 182 WLSGPEFTVAILGEEILPSVRIQPSGT-FYDYEAKYL---SDETQYFCPAGLEASQEANL 237 Query: 231 HAAALACYQALKCTVYARVD-MLVKDGIPYVLEVNTLPGMTETSLLPRSALAAGYTFSGL 289 A L + L C + R+D ML DG Y+LE NT PGMT SL+P +A AG +FS L Sbjct: 238 QALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQL 297 Query: 290 LDEII 294 + I+ Sbjct: 298 VVRIL 302 >ddlB [M] COG1181 D-alanine-D-alanine ligase and related ATP-grasp enzymes Length = 306 Score = 199 bits (507), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 128/305 (41%), Positives = 175/305 (57%), Gaps = 19/305 (6%) Query: 3 KVGVIMGGVSSEYEVSLNTGREMLKHLDRSKYEGVPVVIHSREELVDAVKGLDF--ALLA 60 K+ V++GG S+E EVSLN+G +L L + PV +E V +K + F +A Sbjct: 4 KIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPV--DPKEVDVTQLKSMGFQKVFIA 61 Query: 61 LHGAYGEDGTVQGTLETLGIPYSGSGVLASSLCMDKHLSKTIIRSKGVHTPDWLCLDRME 120 LHG GEDGT+QG LE +G+PY+GSGV+AS+L MDK SK + + G+ W+ L R E Sbjct: 62 LHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKLLWQGAGLPVAPWVALTRAE 121 Query: 121 ------DLSPEAVEALGYPVMVKPNSGGSSIGMTKVNSSAELRSAVEKAFAADCSVLIEE 174 D + ALG PV+VKP+ GSS+GM+KV + L+ A+ AF D VLIE+ Sbjct: 122 FEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAENALQDALRLAFQHDEEVLIEK 181 Query: 175 YTEGREITCPILGGTLLPVIGIRSLGADWFDYSAKYELGGAEERVIQLPAEL----EERV 230 + G E T ILG +LP I I+ G ++DY AKY ++E PA L E + Sbjct: 182 WLSGPEFTVAILGEEILPSIRIQPSGT-FYDYEAKYL---SDETQYFCPAGLEASQEANL 237 Query: 231 HAAALACYQALKCTVYARVD-MLVKDGIPYVLEVNTLPGMTETSLLPRSALAAGYTFSGL 289 A L + L C + R+D ML DG Y+LE NT PGMT SL+P +A AG +FS L Sbjct: 238 QALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQL 297 Query: 290 LDEII 294 + I+ Sbjct: 298 VVRIL 302 Score = 199 bits (507), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 127/305 (41%), Positives = 176/305 (57%), Gaps = 19/305 (6%) Query: 3 KVGVIMGGVSSEYEVSLNTGREMLKHLDRSKYEGVPVVIHSREELVDAVKGLDF--ALLA 60 K+ V++GG S+E EVSLN+G +L L + PV +E V +K + F +A Sbjct: 4 KIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPV--DPKEVDVTQLKSMGFQKVFIA 61 Query: 61 LHGAYGEDGTVQGTLETLGIPYSGSGVLASSLCMDKHLSKTIIRSKGVHTPDWLCLDRME 120 LHG GEDGT+QG LE +G+PY+GSGV+AS+L MDK SK + + G+ W+ L R+E Sbjct: 62 LHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKLLWQGAGLPVAPWVALTRVE 121 Query: 121 ------DLSPEAVEALGYPVMVKPNSGGSSIGMTKVNSSAELRSAVEKAFAADCSVLIEE 174 D + ALG PV+VKP+ GSS+GM+KV + L+ A+ AF D VLIE+ Sbjct: 122 FEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAENALQDALRLAFQHDEEVLIEK 181 Query: 175 YTEGREITCPILGGTLLPVIGIRSLGADWFDYSAKYELGGAEERVIQLPAEL----EERV 230 + G E T ILG +LP + I+ G ++DY AKY ++E PA L E + Sbjct: 182 WLSGPEFTVAILGEEILPSVRIQPSGT-FYDYEAKYL---SDETQYFCPAGLEASQEANL 237 Query: 231 HAAALACYQALKCTVYARVD-MLVKDGIPYVLEVNTLPGMTETSLLPRSALAAGYTFSGL 289 A L + L C + R+D ML DG Y+LE NT PGMT SL+P +A AG +FS L Sbjct: 238 QALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQL 297 Query: 290 LDEII 294 + I+ Sbjct: 298 VVRIL 302 >ECs0096 [M] COG1181 D-alanine-D-alanine ligase and related ATP-grasp enzymes Length = 306 Score = 199 bits (507), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 127/305 (41%), Positives = 176/305 (57%), Gaps = 19/305 (6%) Query: 3 KVGVIMGGVSSEYEVSLNTGREMLKHLDRSKYEGVPVVIHSREELVDAVKGLDF--ALLA 60 K+ V++GG S+E EVSLN+G +L L + PV +E V +K + F +A Sbjct: 4 KIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPV--DPKEVDVTQLKSMGFQKVFIA 61 Query: 61 LHGAYGEDGTVQGTLETLGIPYSGSGVLASSLCMDKHLSKTIIRSKGVHTPDWLCLDRME 120 LHG GEDGT+QG LE +G+PY+GSGV+AS+L MDK SK + + G+ W+ L R+E Sbjct: 62 LHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKLLWQGAGLPVAPWVALTRVE 121 Query: 121 ------DLSPEAVEALGYPVMVKPNSGGSSIGMTKVNSSAELRSAVEKAFAADCSVLIEE 174 D + ALG PV+VKP+ GSS+GM+KV + L+ A+ AF D VLIE+ Sbjct: 122 FEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAENALQDALRLAFQHDEEVLIEK 181 Query: 175 YTEGREITCPILGGTLLPVIGIRSLGADWFDYSAKYELGGAEERVIQLPAEL----EERV 230 + G E T ILG +LP + I+ G ++DY AKY ++E PA L E + Sbjct: 182 WLSGPEFTVAILGEEILPSVRIQPSGT-FYDYEAKYL---SDETQYFCPAGLEASQEANL 237 Query: 231 HAAALACYQALKCTVYARVD-MLVKDGIPYVLEVNTLPGMTETSLLPRSALAAGYTFSGL 289 A L + L C + R+D ML DG Y+LE NT PGMT SL+P +A AG +FS L Sbjct: 238 QALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQL 297 Query: 290 LDEII 294 + I+ Sbjct: 298 VVRIL 302 >STM0130 [M] COG1181 D-alanine-D-alanine ligase and related ATP-grasp enzymes Length = 306 Score = 197 bits (501), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 130/305 (42%), Positives = 178/305 (58%), Gaps = 19/305 (6%) Query: 3 KVGVIMGGVSSEYEVSLNTGREMLKHLDRSKYEGVPVVIHSREELVDAVKGLDF--ALLA 60 K+ V++GG S+E +VSLN+G +L L PV +E V +K + F +A Sbjct: 4 KIAVLLGGTSAERDVSLNSGAAVLAGLREGGINAHPV--DPQEVDVAQLKAMGFQKVFIA 61 Query: 61 LHGAYGEDGTVQGTLETLGIPYSGSGVLASSLCMDKHLSKTIIRSKGVHTPDWLCLDRME 120 LHG GEDGT+QG LE LG+PY+GSGV+AS+L MDK SK + + G+ W+ L R E Sbjct: 62 LHGRGGEDGTLQGMLELLGLPYTGSGVMASALSMDKLRSKLLWQGAGLPVAPWVALTRAE 121 Query: 121 ---DLSPEA---VEALGYPVMVKPNSGGSSIGMTKVNSSAELRSAVEKAFAADCSVLIEE 174 LS E + ALG P++VKP+ GSS+GMTKV L+ A+ AF D +LIE+ Sbjct: 122 FEKGLSEEQKARISALGLPLIVKPSREGSSVGMTKVVEENALQGALSLAFQHDDEILIEK 181 Query: 175 YTEGREITCPILGGTLLPVIGIRSLGADWFDYSAKYELGGAEERVIQLPAELEERVHAA- 233 + G E T I+G +LP I I+ G ++DY AKY ++E PA L+ AA Sbjct: 182 WLCGPEFTVAIVGEEILPSIRIQPAGT-FYDYEAKYL---SDETQYFCPAGLDASQEAAL 237 Query: 234 ---ALACYQALKCTVYARVD-MLVKDGIPYVLEVNTLPGMTETSLLPRSALAAGYTFSGL 289 L ++AL CT + R+D ML DG Y+LE NT PGMT SL+P +A AG +FS L Sbjct: 238 QSLVLQAWKALGCTGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQL 297 Query: 290 LDEII 294 + I+ Sbjct: 298 VVRIL 302 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,251,420 Number of extensions: 1058420 Number of successful extensions: 5103 Number of sequences better than 1.0e-05: 33 Number of HSP's gapped: 5059 Number of HSP's successfully gapped: 33 Length of query: 333 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 226 Effective length of database: 26,037,693 Effective search space: 5884518618 Effective search space used: 5884518618 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)