BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0517.0005.p (523 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic ... 114 4e-25 BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic... 103 8e-22 BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic ... 101 3e-21 BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic... 69 2e-11 YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic... 54 8e-07 >BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 550 Score = 114 bits (285), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 123/493 (24%), Positives = 201/493 (40%), Gaps = 81/493 (16%) Query: 76 EEKFNVKIKPVKVTDASIASGE-VPDIFMLGDPANVTAYQNQGVLMNLDQNMLKEKMPEY 134 +E+FN +++ + ++SGE +PD+F + D A + G++M +D+ + P Sbjct: 87 DEQFNTQLRLM------MSSGEKLPDVFQVNDTAIKADLLSSGLVMPVDEAIETYASPRL 140 Query: 135 YADIEKNNALFQTVTIDDKLWAIPMFIDLKPYDLGMLWRKDWLDKVGITKAPETLDEFEK 194 E+ F T D + IP F D + R+DWLDK + + P TL+E E Sbjct: 141 KQLFEQFPQAFYPSTKDGVRYGIPRFSGGNGSDTLLWIRQDWLDKFDL-EPPTTLEELEH 199 Query: 195 AIYAFAQKDPDGNGQKDTYGLT-----------GTATSTWSSGFYSIFGAFG-VEPTMWH 242 + F +DP+ G+ DT G+T A S+W +FGAFG P W Sbjct: 200 IMDVFVNEDPNETGKNDTIGITFAAGDYGFRGGNIADSSW------VFGAFGDFVPGAWS 253 Query: 243 E-KNGEIMNGSVMPETKEALAKLRQWFADGVIDPEFITDTQDSYRKKLYNNRIGVIEEQI 301 + +G ++ GS+ P K+ LAK+ +W+ G + +F + ++ + R G++ Sbjct: 254 KGDDGSLVYGSIQPSMKDGLAKIHEWYEKGYLATDFAILSAGDAQESFISGRSGIMAAPP 313 Query: 302 AKGALPDAATVTEMKSLNPSAELVFTKNPKGPGGDGSWDWGVKSNFIVISNKVKDQPEKL 361 P + EM + P A + + GP G ++V S E + Sbjct: 314 WAHGYP----IGEMAQVVPDAIVKPFPHVSGPNGQAGRR---GEGYVVGSFLFNKDFEHM 366 Query: 362 NKLFEILRAESTDEETINMTSLGVKGTHWDFTESGATSGATQF---------KAGFEK-- 410 +K FE A I + G D+ E G G A FE+ Sbjct: 367 DKFFEYWDA-------IYGYTFGES----DYFEYGLFEGYDYVMEDGEPVYDHAQFEEIT 415 Query: 411 AEERDQAGIRLFSLGNITT---------QAYREKYSNPALN----------EAIKTYSSA 451 EE G R F N+ T + + E P + I+ + Sbjct: 416 GEEVVDPG-RYFLPTNVPTIPFEMYSLLEEFHETGREPETGYEAGLAGRDPDYIEAAAIV 474 Query: 452 PRQTDALLFSAL-----PSDGKYKNDLTSLMQKYFAQIISGEIPLEDFDKFVAEWKSRGG 506 RQ D + + P+ L + + F II G PL+ FD+FV EW+ GG Sbjct: 475 NRQNDLRIINEFNGPPTPTMVSRSEHLERMEDELFVDIIYGNKPLDAFDQFVEEWRQSGG 534 Query: 507 DELTKEANEMYQA 519 + +T+E NE Y++ Sbjct: 535 EAITEEVNEWYES 547 >BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 502 Score = 103 bits (256), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 117/478 (24%), Positives = 203/478 (42%), Gaps = 66/478 (13%) Query: 73 KWLEEKFNVKIKPVKVTDAS--------IASGEVPDIFMLGDPANVTAYQNQGVLMNLDQ 124 K LE+ +IK V D+S +ASGE+P ++ D ++ G + ++ Sbjct: 58 KALEKYTGKEIKVTWVPDSSYNDKFNIVMASGEMPHAIVIKD-------KSAGFIKSVKA 110 Query: 125 NMLKEKMP---EYYADIEKNNALFQTVTIDDKLWAIPMFIDLKPYDLGMLWRKDWLDKVG 181 E P +Y + + + + +++ +++ I DL M+ R DWL VG Sbjct: 111 GAFWELSPYLKDYKNLSQADEKILKNSSVNGEVYGIYRTRDL--IRACMIIRTDWLKNVG 168 Query: 182 ITKAPETLDEFEKAIYAFAQKDPDGNGQKDTYGLT-----GTATSTWSSGFYSIFGAFGV 236 + PETLD+F + + AF +KDPDGNG+ DTYG+ G + FGA Sbjct: 169 LD-MPETLDDFYEVLKAFKEKDPDGNGKDDTYGMVVPKWMGLGNGSPWDVLQIWFGA--- 224 Query: 237 EPTMWHEKNGEIMNGSVMPETKEALAKLRQWFADGVIDPEFITDTQDSYRKKLYNNRIGV 296 P + +NG+++ E +AL ++ + +G+I+ +F + + + GV Sbjct: 225 -PNRYGVENGKLIPDFTTKEYMDALTFFKKLYDEGLINKDFAVMDSAKWNDPVVKGKAGV 283 Query: 297 IEEQIAKGALPDAATVTEMKSLNPSAELVFTKNPKGPGGDGSWDWGVKSNFIVISNKVKD 356 I + ++ + +A +S ++V + +GP G ++ S I I Sbjct: 284 IVDTGSRASQIQSAMEEADESNKDIIDIVGS--LEGPNGKRTFPTSGYSGMITIPKSSVK 341 Query: 357 QPEKLNKLFEILRAESTDEETINMTSLGVKGTHWDFTESGATS-------------GATQ 403 ++L ++ L + D+E +T+ GVKG +++ + TS G Q Sbjct: 342 TEKELKEVLSFLD-KMNDKEAQILTNNGVKGRNYELKDGVFTSLEKNNKSLLYEHEGLAQ 400 Query: 404 FKAGFEKAEE--RDQAGIRLFSLGNITTQAYREKYSNPALNEAIKTYSSAPRQTDALLFS 461 F K+E DQ +I T+ + NPA + Y+ Q D ++ Sbjct: 401 FSMSIPKSEYYIEDQKTKLFQHRKDIITEGEKIAVFNPAESLVSDVYTQKGAQLDNIILD 460 Query: 462 ALPSDGKYKNDLTSLMQKYFAQIISGEIPLEDFDKFVAEWKSRGGDELTKEANEMYQA 519 A Q I GEI + FD V WK GG+EL K+ N++YQ+ Sbjct: 461 AR------------------TQFIIGEIDEKGFDDAVELWKKSGGNELMKDLNKLYQS 500 >BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 512 Score = 101 bits (252), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 114/500 (22%), Positives = 210/500 (42%), Gaps = 69/500 (13%) Query: 49 EAPMEITWGI---HLQAPGVVENSQVQKWLEEKFNVKIKPVKVTDA--------SIASGE 97 ++P+++ W + H Q P V K +EE N + + V DA ++ASG Sbjct: 49 DSPLDLDWMVILYHQQPP----KDTVIKEIEELTNTNLDLIWVPDAVKEDRVNAALASGN 104 Query: 98 VPDIFMLGDPANVTAYQNQGVLMNLDQNMLKEKMP---EYYADIEKNNALFQTVTIDDKL 154 +P + L D ++ VL E P E+ E N + + V++D K+ Sbjct: 105 LPKVITLQD------FRTSSVLNAFRSGQFWEIGPYLDEFPNLSEMNETILENVSVDGKI 158 Query: 155 WAIPMFIDLKPYDLGMLWRKDWLDKVGITKAPETLDEFEKAIYAFAQKDPDGNGQKDTYG 214 + I + + G++ R+DWL+ +G+ + P L+E + AF DP+G+G DT G Sbjct: 159 YGI--YRERPLSRQGVVIRQDWLENLGL-EMPTNLEELYEVARAFTYDDPNGSGADDTVG 215 Query: 215 LTGTATSTWSSGFYSIFGAFGVEPTMW-HEKNGEIMNGSVMPETKEALAKLRQWFADGVI 273 T + G + ++ P W + G++M + E E + ++ + +G+I Sbjct: 216 FTDRNDLIF--GAFKTLASYHGTPNEWGFDDEGKLMPEFMTDEYVETMNYMKSLYDEGLI 273 Query: 274 DPEFITDTQDSYRKKLYNNRIGVIEEQIAKGALPDAATVTE-MKSLNPSAELVFTKNPKG 332 + EF +++ ++ R G I G + DA + + K +NP E+ T G Sbjct: 274 NTEFPVTSKEQQQELFMTGRAG-----IYVGNMVDARNLRDKAKDINPEMEVAITNRIAG 328 Query: 333 PGG-DGSWDWGVKSNFIVISNKVKDQPEKLNKLFEILRAESTDEETINMTSLGVKGTHWD 391 P G D W G + I + +L ++ +E+ N+ + G++ H++ Sbjct: 329 PDGEDRVWAQGGHNGIFAIPKSSVETEAELKRILAFF-DRIAEEDVYNLMAYGIEDVHYE 387 Query: 392 FTESGATSGATQFKAGFEKAEER-DQAGIRLFSLGNITTQA--YREKYSNPAL------- 441 + FE+ EE D + L ++ + Y + Y +P Sbjct: 388 TIDGEY----------FERIEENLDDWAAEVQPLVSLVAREMNYLKAYGDPLYEEYERLT 437 Query: 442 ---NEAIKTYSSAPRQTDALLFSALPSDGKYKNDLTSLMQKYFAQIISGEIPLEDFDKFV 498 NE I T + P LFS P+ + +L ++ + I GE+ + F+ V Sbjct: 438 EDNNEIIVTNPAEP------LFS--PTYTERGTELQKIIDDATYKYILGELDEDGFEAEV 489 Query: 499 AEWKSRGGDELTKEANEMYQ 518 W + GG+ + +E NE Y+ Sbjct: 490 ERWLTNGGESIIEELNEDYE 509 >BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 440 Score = 69.3 bits (168), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 83/345 (24%), Positives = 161/345 (46%), Gaps = 34/345 (9%) Query: 59 HLQAPGVVENSQVQKWLEEKFNVKIKPVKVT----DASIASGEVPDIFMLGDPANVTAYQ 114 H Q P +++K + ++ P V +A++A+G +P I + D N + Sbjct: 42 HQQPPKDRAIKEIEKLTNTELDITWVPDAVKEDRLNAALAAGNLPQIVTIQDIKNSS--- 98 Query: 115 NQGVLMNLDQNMLKEKMPEY---YADIEKNNALF-QTVTIDDKLWAIPMFIDLKPYDLGM 170 +MN ++ + ++ +Y Y ++ K N L + VTID KL+ I + + G+ Sbjct: 99 ----VMNAFRSGMFWEIGDYIKDYPNLNKMNKLINKNVTIDGKLYGI--YRERPLSRQGI 152 Query: 171 LWRKDWLDKVGITKAPETLDEFEKAIYAFAQKDPDGNGQKDTYGLTGTATSTWSSGFYSI 230 + RKDWLD + + K P+TLDE + AF + DPD +G+ DT+GL + G + Sbjct: 153 VIRKDWLDNLNL-KTPKTLDELYEVAKAFTEDDPDKDGKDDTFGLADRNDLIY--GAFKT 209 Query: 231 FGAFGVEPTMWHEKNGEIMNGSVMPETKEALAKLRQWFADGVIDPEFITDTQDSYRKKLY 290 G++ PT W E G+ + E K+ + +++ +G ++ +F ++ ++ Sbjct: 210 IGSYEGMPTDWKESGGKFTPDFMTQEYKDTMNYMKKLRDNGYMNKDFPVTSKTQQQELFS 269 Query: 291 NNRIGVIEEQIAKGALPDAATVTEMKSLNPSAELVFTKNPKGPGG-DGSWDWGVKSN--- 346 + G I G + DA + + S + S +L KGP G + W G + Sbjct: 270 QGKAG-----IYIGNMVDAVNLRDHAS-DKSMKLEIINRIKGPDGKERVWASGGHNGVFA 323 Query: 347 FIVISNKVKDQPEKLNKLFEILRAESTDEETINMTSLGVKGTHWD 391 F S K + + +++ F+ + +E+ ++ + G+ G H++ Sbjct: 324 FPKTSVKTEAELKRILAFFDRI----AEEDVYSLMTYGIDGVHYN 364 >YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 437 Score = 53.5 bits (127), Expect = 8e-07, Method: Compositional matrix adjust. Identities = 59/225 (26%), Positives = 90/225 (40%), Gaps = 27/225 (12%) Query: 150 IDDKLWAIPMFIDLKPYDL-------GMLWRKDWLDKVGITKAPETLDEFEKAIYAFAQK 202 I D W +ID K Y + + RKDW +K+G+ + P+ + AF Sbjct: 119 IFDTAWKSTQYIDGKYYGVPTSAQTFALFVRKDWREKLGLEQ-PKNWQDISTLAKAFTFN 177 Query: 203 DPDGNGQKDTYG--LTGTATSTWSSGFYSIF----GAFGVEPTMWHEKNGEIMNGSVMPE 256 DPDGNG+ DTYG L G+ T ++S F S + G + P G+ + P Sbjct: 178 DPDGNGKNDTYGFILPGSTTRGYASWFISSYLWQAGGDFIRPA----GEGKFIASLEEPA 233 Query: 257 TKEALAKLRQWFADGVIDPEFITDTQDSYRKKLYNNRIGVIEEQIAKGALPDAATVTEMK 316 E L+ +R D + P I T + + G+ AL D TE Sbjct: 234 ATETLSFMRGMVCDKTVQPGAINATTADAIPSFRSGQSGMFFSGPYHIALFDKDPGTEAF 293 Query: 317 SLNPSAELVFTKNPKGPGGDGSWDWGVKSNFIVISNKVKDQPEKL 361 + P P GP G + G + F++ S++ K +K Sbjct: 294 EVIPP--------PTGPKGQATLAEGT-TVFMMKSSQKKAAAQKF 329 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.131 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,458,357 Number of extensions: 1645816 Number of successful extensions: 2490 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 2483 Number of HSP's successfully gapped: 7 Length of query: 523 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 412 Effective length of database: 25,576,357 Effective search space: 10537459084 Effective search space used: 10537459084 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 118 (50.1 bits)