BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0515.0033.p (263 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1273 [M] COG3475 LPS biosynthesis protein 104 2e-22 SP1274 [M] COG3475 LPS biosynthesis protein 94 2e-19 SP1367 [M] COG3475 LPS biosynthesis protein 74 3e-13 RC1054 [M] COG3475 LPS biosynthesis protein 52 7e-07 RC1055 [M] COG3475 LPS biosynthesis protein 50 4e-06 >SP1273 [M] COG3475 LPS biosynthesis protein Length = 267 Score = 104 bits (259), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 74/252 (29%), Positives = 129/252 (51%), Gaps = 20/252 (7%) Query: 15 LSPEDLRAVQLIQVEMLAEVDRICRLHHINYAMIGGTMLGAIRHKGYIPWDDDADIGLLR 74 L+ ED + ++L E+L +D +C+ H+INY + GT++GA+RH+G+IPWDDD D+ + R Sbjct: 4 LTVEDAKQIEL---EILDYIDTLCKKHNINYIINYGTLIGAVRHEGFIPWDDDIDLSMPR 60 Query: 75 EEYEKFRKVCETDLDHERFYFQDMRATPGYRWGYGKIRRKNTYFLREGQAHLPYDSGIFI 134 E+Y++F + + + ++ + Y + KI T + + + Y+SGIFI Sbjct: 61 EDYQRFINIFQK--EKSKYKLLSLETDKNYFNNFIKITDSTTKII-DTRNTKTYESGIFI 117 Query: 135 DIFPFDSVPDGKLAEKMHNLHCTIIRKILWSEVGKKTDKNPML----RYVFSLLSLIPLG 190 DIFP D D K+ + + L + + +S+ K+ +L R F LL L P+ Sbjct: 118 DIFPIDRFDDPKVIDTCYKLES--FKLLSFSKHKNIVYKDSLLKDWIRTAFWLL-LRPVS 174 Query: 191 --MVSNHLQHFAGKSNRKKYQRVRVL--TFPARDNVSEGRREWYEKTVDYKFEDLDLLGA 246 +N ++ K +R+ Q + + F ++ G ++KT+D FE+L L Sbjct: 175 PRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGT---FDKTIDLPFENLSLPAP 231 Query: 247 ESADAYLKHKFG 258 E D L +G Sbjct: 232 EKFDTILTQFYG 243 >SP1274 [M] COG3475 LPS biosynthesis protein Length = 269 Score = 94.0 bits (232), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 54/173 (31%), Positives = 90/173 (52%), Gaps = 4/173 (2%) Query: 21 RAVQLIQVEMLAEVDRICRLHHINYAMIGGTMLGAIRHKGYIPWDDDADIGLLREEYEKF 80 + ++ IQ+ +L +D C+ H I Y + GTMLGAIRHKG IPWDDD DI L RE+YE+ Sbjct: 7 KEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRHKGMIPWDDDIDISLYREDYERL 66 Query: 81 RKVCETDLDHERFYFQDMRATPGYRWGYGKIRRKNTYFLREGQAHLPYDSGIFIDIFPFD 140 K+ E + +H R+ + Y + I +T + + + +D+ +FID+FP D Sbjct: 67 LKIIEEE-NHPRYKVLSYDTSSWYFHNFASILDTST-VIEDHVKYKRHDTSLFIDVFPID 124 Query: 141 SVPDGKLAEKMHNLHCTIIRKILWSEVGKKTDKNPMLRYVFSLLSLIPLGMVS 193 D + +K + +R++ + + + + L+ L S L V+ Sbjct: 125 RFTDLSIVDKSYKY--VALRQLAYIKKSRAVHGDSKLKDFLRLCSWYALRFVN 175 >SP1367 [M] COG3475 LPS biosynthesis protein Length = 281 Score = 73.6 bits (179), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 64/248 (25%), Positives = 103/248 (41%), Gaps = 15/248 (6%) Query: 19 DLRAVQLIQVEMLAEVDRICRLHHINYAMIGGTMLGAIRHKGYIPWDDDADIGLLREEYE 78 DL+A+Q +EM C+ H + + GG +GA+R+KG+IPWDDD D + R++YE Sbjct: 6 DLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALRNKGFIPWDDDLDFFMPRKDYE 65 Query: 79 KFRKVCETDLDHERFYFQDMRATPGYRWGYGKIRRKNTYFLREGQAHLPYDSGIFIDIFP 138 K ++ D ER++ R + IR K T ++ Q L G+ +D+ P Sbjct: 66 KLAELWPRYAD-ERYFLSKSHKDFVDRNLFITIRDKKTTCIKPYQQDLDLPHGLALDVLP 124 Query: 139 FDSVPDGKLAEKMHNLHCTIIRKILWSEVGKKTDKNPMLRYVFSLLSLIPLGMVSNHLQH 198 D P AE+ + +I + ++ + + M S I LG+ L++ Sbjct: 125 LDYYPKNP-AERKKQVRWALIYSLFCAQTIPEKHGDLM-----KWGSRILLGLTPKSLRY 178 Query: 199 FAGKSNRKKYQRVRVLTFPARDNVSEGRREWYEKTVDYKFEDLDLLGAESA--------D 250 K K+ + + + G K FED L E D Sbjct: 179 RIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSFEDNLFLPFEGTEMPIPIGYD 238 Query: 251 AYLKHKFG 258 YL+ FG Sbjct: 239 VYLRTAFG 246 >RC1054 [M] COG3475 LPS biosynthesis protein Length = 250 Score = 52.4 bits (124), Expect = 7e-07, Method: Compositional matrix adjust. Identities = 27/93 (29%), Positives = 52/93 (55%), Gaps = 10/93 (10%) Query: 21 RAVQLIQVEMLAEVDRICRLHHINYAMIGGTMLGAIRHKGYIPWDDDADIGLLREEYEKF 80 +A+ L Q ++ + + ++INY + GT+LGA+RH+G IP+DDD DIG++ E+ + Sbjct: 54 KAIALYQ--LMKDTHELLGKNNINYWIDSGTLLGAVRHQGIIPFDDDLDIGIMHEDEIRL 111 Query: 81 RKVCETDLDHERFYFQDMRATPGYRWGYGKIRR 113 +++ F+ + T Y+ Y ++ Sbjct: 112 QQILPQ--------FEQLGYTVSYKRTYNICKK 136 >RC1055 [M] COG3475 LPS biosynthesis protein Length = 266 Score = 50.1 bits (118), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 22/63 (34%), Positives = 42/63 (66%), Gaps = 2/63 (3%) Query: 21 RAVQLIQVEMLAEVDRICRLHHINYAMIGGTMLGAIRHKGYIPWDDDADIGLLREEYEKF 80 +A+ L Q ++ + + ++INY + GT+LGA+RH+G IP+DDD DI ++ E+ + Sbjct: 54 KAIALYQ--LMKDTHELLGKNNINYWINSGTLLGAVRHQGIIPFDDDLDISIMHEDEIRL 111 Query: 81 RKV 83 +++ Sbjct: 112 QQI 114 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.141 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,857,367 Number of extensions: 891527 Number of successful extensions: 1088 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 1084 Number of HSP's successfully gapped: 7 Length of query: 263 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 159 Effective length of database: 26,383,695 Effective search space: 4195007505 Effective search space used: 4195007505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)