BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0515.0012.p (275 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3604 [S] COG1284 Uncharacterized conserved protein 256 4e-68 BS_yvjA [S] COG1284 Uncharacterized conserved protein 244 8e-65 CAC0848 [S] COG1284 Uncharacterized conserved protein 239 5e-63 lin2652 [S] COG1284 Uncharacterized conserved protein 232 5e-61 SPy2209 [S] COG1284 Uncharacterized conserved protein 162 5e-40 >BH3604 [S] COG1284 Uncharacterized conserved protein Length = 293 Score = 256 bits (653), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 137/256 (53%), Positives = 180/256 (70%), Gaps = 1/256 (0%) Query: 16 AFNLFFLPNRIASGGVSGLSVLAEAWLGAEPAFTQWALNIPLFILGVVILGK-QYGLRSL 74 AFNLF LPN++ASGGVSG+S+L LG EPA+ QWA NIPLF+LGV +LG +YG+++L Sbjct: 37 AFNLFLLPNQVASGGVSGISILINYTLGLEPAYVQWAFNIPLFLLGVWLLGGLKYGIKAL 96 Query: 75 LGSFVLPLFIFLTKDGPVPTTNPLLASIYXXXXXXXXXXXXXXXXXSTGGLSILAQIIQK 134 +G+ LPL + LTKD P T +PLL +I+ STGG + AQI+ K Sbjct: 97 IGTSFLPLVVLLTKDLPPATLDPLLGAIFGGMGVGLGLGIVFRARASTGGTDLAAQIVHK 156 Query: 135 ITGFSFSLSVVLLDGTVITLAAFVLGMEQAMYALIGLFVTGKVINALEVGFSTTKVAYII 194 TG S + V ++DG ++ AAF+ G+E A+YALI LFVTGK I+ +++G KVA II Sbjct: 157 YTGLSVGMCVFMIDGLIVMSAAFLFGIEYALYALIALFVTGKTIDLVQIGVGYAKVALII 216 Query: 195 SDQTEEISQAILNDLDRGLTKLNAQGGYTGDSRTVLMVVVGQNEITRLKALVRSVDPGAF 254 S+Q EE+ + IL D+DRG+TKL GGYT R VLM VVGQ+E+T+LK +V+S+DP AF Sbjct: 217 SNQQEEVRKGILQDIDRGVTKLVGYGGYTNQERPVLMCVVGQSEVTKLKQIVQSIDPQAF 276 Query: 255 VIITEAHEVLGEGFKR 270 VI+T A EVLGEGFKR Sbjct: 277 VIVTSATEVLGEGFKR 292 >BS_yvjA [S] COG1284 Uncharacterized conserved protein Length = 281 Score = 244 bits (624), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 117/255 (45%), Positives = 176/255 (69%), Gaps = 1/255 (0%) Query: 16 AFNLFFLPNRIASGGVSGLSVLAEAWLGAEPAFTQWALNIPLFILGVVILGKQYGLRSLL 75 +FN+F LPN+IA+GGVSG+S + +++ G E A+ QW +NIPLFI GV++LG ++GL++L Sbjct: 27 SFNVFLLPNKIAAGGVSGISTILQSY-GFEAAYVQWIINIPLFIAGVILLGGKFGLKTLA 85 Query: 76 GSFVLPLFIFLTKDGPVPTTNPLLASIYXXXXXXXXXXXXXXXXXSTGGLSILAQIIQKI 135 GS LPL +FLT+D T + LLA+I+ STGG ++ AQII K Sbjct: 86 GSVFLPLVVFLTRDIQPATHHELLAAIFGGVGIGIGIGIVYLGKGSTGGTALAAQIIHKY 145 Query: 136 TGFSFSLSVVLLDGTVITLAAFVLGMEQAMYALIGLFVTGKVINALEVGFSTTKVAYIIS 195 +G S + ++DG ++ A V +EQ +YA++G++V+ K I+ ++VGF+ +K+A II+ Sbjct: 146 SGLSLGKCLAIIDGMIVVTAMIVFNIEQGLYAMLGVYVSSKTIDVVQVGFNRSKMALIIT 205 Query: 196 DQTEEISQAILNDLDRGLTKLNAQGGYTGDSRTVLMVVVGQNEITRLKALVRSVDPGAFV 255 Q + + +A+L +DRG+TK++A GGYT D R +LM VVGQ E T+LK +V+ +D AFV Sbjct: 206 KQEQAVKEAVLQKIDRGVTKISAVGGYTDDDRPILMCVVGQTEFTKLKQIVKQIDESAFV 265 Query: 256 IITEAHEVLGEGFKR 270 I+ +A EVLGEGFKR Sbjct: 266 IVADASEVLGEGFKR 280 >CAC0848 [S] COG1284 Uncharacterized conserved protein Length = 292 Score = 239 bits (609), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 122/253 (48%), Positives = 166/253 (65%) Query: 17 FNLFFLPNRIASGGVSGLSVLAEAWLGAEPAFTQWALNIPLFILGVVILGKQYGLRSLLG 76 FNLF P++IASGG+ GLS+L E EPA TQW NIP+F LG+ LGK++ L+S LG Sbjct: 34 FNLFLSPSKIASGGMVGLSLLIEQLFHIEPAITQWVGNIPIFFLGLFFLGKRFTLKSALG 93 Query: 77 SFVLPLFIFLTKDGPVPTTNPLLASIYXXXXXXXXXXXXXXXXXSTGGLSILAQIIQKIT 136 + +LPLFI +TK+ P T+N LLASIY STGG SI+A I K T Sbjct: 94 TVILPLFILITKNLPPITSNALLASIYGGVLSGIGLGLVFKGNGSTGGTSIIASIFNKFT 153 Query: 137 GFSFSLSVVLLDGTVITLAAFVLGMEQAMYALIGLFVTGKVINALEVGFSTTKVAYIISD 196 G S S +D VI + F+ E+A+YA++ LF+ GKVI+ +++GF ++ VA++ISD Sbjct: 154 GVSIGNSQAFIDAVVIIASGFIFNAEKALYAILSLFIIGKVIDLVQLGFKSSIVAFVISD 213 Query: 197 QTEEISQAILNDLDRGLTKLNAQGGYTGDSRTVLMVVVGQNEITRLKALVRSVDPGAFVI 256 + E+I Q I+ +LDRG+TKL+ GGYT + +TVLMVV+ E+ + K LV D AFVI Sbjct: 214 KAEDIRQGIIEELDRGITKLSGCGGYTDEEKTVLMVVMEHTEVNKFKKLVYEKDKNAFVI 273 Query: 257 ITEAHEVLGEGFK 269 I+ HEVLG+GF Sbjct: 274 ISNTHEVLGQGFN 286 >lin2652 [S] COG1284 Uncharacterized conserved protein Length = 287 Score = 232 bits (591), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 111/253 (43%), Positives = 164/253 (64%) Query: 16 AFNLFFLPNRIASGGVSGLSVLAEAWLGAEPAFTQWALNIPLFILGVVILGKQYGLRSLL 75 AFN+ LPN +ASGGVSG+S + G PAF QWA NIPLF+ G+ LG Q+GL++ + Sbjct: 33 AFNVLLLPNHVASGGVSGISTIINYLTGWNPAFIQWAFNIPLFLAGLFFLGYQFGLKTFV 92 Query: 76 GSFVLPLFIFLTKDGPVPTTNPLLASIYXXXXXXXXXXXXXXXXXSTGGLSILAQIIQKI 135 G+ +LP F++LT+ T PL+A+++ STGG + AQI+ K Sbjct: 93 GTMLLPFFVYLTQGLAPWTHEPLVAALFGGVLVGMGLGIVFRGKASTGGTDVAAQILHKY 152 Query: 136 TGFSFSLSVVLLDGTVITLAAFVLGMEQAMYALIGLFVTGKVINALEVGFSTTKVAYIIS 195 + + + V L+DG V+ A FV +E +YALIGLF T K I+ ++VG + +K +IIS Sbjct: 153 SHLTLGICVALIDGFVVISAMFVFDIESGLYALIGLFATSKTIDLVQVGLNQSKTVFIIS 212 Query: 196 DQTEEISQAILNDLDRGLTKLNAQGGYTGDSRTVLMVVVGQNEITRLKALVRSVDPGAFV 255 + E I QAIL +DRG+T+L+A+GGYT D + +L+ V+ Q+E +RLK +++ +DP AFV Sbjct: 213 ENQEAIRQAILFKIDRGITRLSAKGGYTEDDKQILLCVIAQSEFSRLKEVIKEIDPEAFV 272 Query: 256 IITEAHEVLGEGF 268 ++ A EV+GEGF Sbjct: 273 VVMSASEVMGEGF 285 >SPy2209 [S] COG1284 Uncharacterized conserved protein Length = 290 Score = 162 bits (410), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 94/261 (36%), Positives = 144/261 (55%), Gaps = 6/261 (2%) Query: 17 FNLFFLPNRIASGGVSGLSVLAEAWLGAEPAFTQWALNIPLFILGVVILGKQYGLRSLLG 76 FN F+ N IASGG+ G++V+ +A G P+ A NIPL ++ LGKQ +++L G Sbjct: 22 FNTMFVDNHIASGGMVGIAVVIKALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYG 81 Query: 77 SFVLPLFIFLTKDGPVPTTNPLLASIYXXXXXXXXXXXXXXXXXSTGGLSILAQIIQKIT 136 S++ P+ I T P T N LLA+I+ STGG IL QI+ K + Sbjct: 82 SWIYPIAIRSTNSLPTLTHNQLLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYS 141 Query: 137 GFSFSLSVVLLDGTVITLAAFVLGMEQAMYALIGLFVTGKVINALEVGFSTTKVAYIISD 196 S +++ ++DG + + L + MY+ IGLFV G VI+ +E GF ++K IIS Sbjct: 142 PLSLGVAMTIVDGISVLMGFIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISK 201 Query: 197 QTEEISQAILNDLDRGLTKLNAQGGYTGDSRTVLMVVVGQNEITRLKALVRSVDPGAFVI 256 + I + I +DRG+TKL +GGYT + +LM +V +E+ L+ + +D AF++ Sbjct: 202 DYQAIREYITTVMDRGVTKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIV 261 Query: 257 ITEAHEVLGEGF------KRE 271 + A +V+G GF KRE Sbjct: 262 VMPAAQVMGRGFSLTKQYKRE 282 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.140 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,413,543 Number of extensions: 678522 Number of successful extensions: 2112 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 2069 Number of HSP's successfully gapped: 30 Length of query: 275 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 170 Effective length of database: 26,268,361 Effective search space: 4465621370 Effective search space used: 4465621370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)