BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0515.0003.p (471 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl2457 [E] COG0165 Argininosuccinate lyase 471 e-132 mlr3506 [E] COG0165 Argininosuccinate lyase 460 e-129 SMc00725 [E] COG0165 Argininosuccinate lyase 457 e-128 SPBC1539.03c [E] COG0165 Argininosuccinate lyase 413 e-115 SPBC1773.14 [E] COG0165 Argininosuccinate lyase 411 e-114 >AGl2457 [E] COG0165 Argininosuccinate lyase Length = 320 Score = 471 bits (1212), Expect = e-132, Method: Compositional matrix adjust. Identities = 0/449 (0%), Positives = 0/449 (0%), Gaps = 1/449 (0%) Query: 2 SKLWGGRFTKGTNKLVEEYTASIGFDKVLAEEDVQGSLAHVTMLGKCGILPQEDVETIKN 61 KL+ ++ V + + + + Sbjct: 31 PHDGTFETQLPGLKLIRCSETTLPMPVVYEPTLCLVAQGRKQAVLGQTSFVYDPSSYLVA 90 Query: 62 GLNKVLGKVRAGEIVFSVADEDIHMNIEKNLIEEIGPVGGKLHTGRSRNDQVATDMHLYL 121 ++ + L + + H + + + Sbjct: 91 SVDLPVMGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQHAPINGPIGLLLNKVDEG 150 Query: 122 RNRVVELVALLHELQEALIGQAKDNVDTIVPGYTHLQRAQPILFAHHLLAYVSMFRRDAE 181 + L + + + I+ + A+V+ R Sbjct: 151 IIDAASRLLALLDTPRDIDALSPLAKREILYRLITGVGGAALRQIVQAGAHVNQIARSIV 210 Query: 182 RLTDSYKRINVLPLGAGALAGTTFPIDRHFVAEQLGFDGVYENSLDAVSDRDFIVEFLAN 241 + ++ + GA + HF A + L R ++ + Sbjct: 211 WIRKHFRETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRTQLRLQEARRLMLSEGLD 270 Query: 242 AALVMTHLSRLSEELVLWSSTEFSFVELDDAFCTGSSIMPQKKNPDVPELVRGKTGRVYG 301 Sbjct: 271 330 Query: 302 NLIGLLTVLKSLPLAYNKDMQEDKEGMFDTVATLTGALQLFAPMISTMKVNKGRMREAVN 361 Sbjct: 331 390 Query: 362 TDFSNATDIADFLVGK-GLPFRQAHEVIGKTVLYCINEGKFLLDLTLEEFKQFSPLFDEQ 420 Sbjct: 391 450 Query: 421 IYAVLQPEAVVNARNVYGGTATVQVKAAI 449 Sbjct: 451 479 >mlr3506 [E] COG0165 Argininosuccinate lyase Length = 466 Score = 460 bits (1184), Expect = e-129, Method: Compositional matrix adjust. Identities = 222/459 (48%), Positives = 305/459 (66%), Gaps = 1/459 (0%) Query: 2 SKLWGGRFTKGTNKLVEEYTASIGFDKVLAEEDVQGSLAHVTMLGKCGILPQEDVETIKN 61 +++WGGRF G ++E ASI FD+ L +D++GS+AH ML + GI+ D E I + Sbjct: 8 NQMWGGRFASGPAAIMEAINASISFDRKLYAQDIRGSIAHSEMLAQTGIISTADQEKIAH 67 Query: 62 GLNKVLGKVRAGEIVFSVADEDIHMNIEKNLIEEIGPVGGKLHTGRSRNDQVATDMHLYL 121 GLN +L ++ AG FS EDIHMN+E L + IG G+LHT RSRNDQVA D+ L++ Sbjct: 68 GLNTILKEIEAGSFEFSTRLEDIHMNVEARLADLIGSAAGRLHTARSRNDQVAVDLRLWV 127 Query: 122 RNRVVELVALLHELQEALIGQAKDNVDTIVPGYTHLQRAQPILFAHHLLAYVSMFRRDAE 181 ++ + L L AL+ +A+++ T++PG+TH+Q AQP+ F HH +AYV MF RD Sbjct: 128 KDECFRVAEALKGLITALLARAEEHAATVMPGFTHMQAAQPVTFGHHCMAYVEMFARDLS 187 Query: 182 RLTDSYKRINVLPLGAGALAGTTFPIDRHFVAEQLGFDGVYENSLDAVSDRDFIVEFLAN 241 R+ D+ +R++ PLGA ALAGT+FPIDRH A+ LGF NSLD+VSDRDF ++FLA Sbjct: 188 RVRDAIERMDESPLGAAALAGTSFPIDRHRTAKALGFREPMRNSLDSVSDRDFALDFLAM 247 Query: 242 AALVMTHLSRLSEELVLWSSTEFSFVELDDAFCTGSSIMPQKKNPDVPELVRGKTGRVYG 301 AA+ THLSRL+EE+++WS+ +F F+ L D+F TGSSIMPQKKNPD ELVRGKTGRV G Sbjct: 248 AAICATHLSRLAEEIIIWSTPQFGFIRLSDSFSTGSSIMPQKKNPDAAELVRGKTGRVNG 307 Query: 302 NLIGLLTVLKSLPLAYNKDMQEDKEGMFDTVATLTGALQLFAPMISTMKVNKGRMREAVN 361 +L+GLLTV+K +PL Y KDMQEDKE +FD TL L M+S M VN M++A Sbjct: 308 HLVGLLTVMKGMPLTYGKDMQEDKESVFDAAETLDLMLAAMTGMVSDMTVNAAAMKKAAG 367 Query: 362 TDFSNATDIADFLVGK-GLPFRQAHEVIGKTVLYCINEGKFLLDLTLEEFKQFSPLFDEQ 420 + + ATD+AD+LV GLPFR+AH V G+ V + L L+LE+ + +P Sbjct: 368 SGHATATDLADWLVRTLGLPFREAHHVTGRAVALAEEKKVSLEKLSLEDLQSINPGITAD 427 Query: 421 IYAVLQPEAVVNARNVYGGTATVQVKAAIERAEAALNEA 459 I++VL + V +R +GGTA +V+ I + L +A Sbjct: 428 IFSVLAVQNSVKSRTSFGGTAPSEVRKQIRYWKKRLAKA 466 >SMc00725 [E] COG0165 Argininosuccinate lyase Length = 467 Score = 457 bits (1176), Expect = e-128, Method: Compositional matrix adjust. Identities = 223/449 (49%), Positives = 301/449 (67%), Gaps = 1/449 (0%) Query: 2 SKLWGGRFTKGTNKLVEEYTASIGFDKVLAEEDVQGSLAHVTMLGKCGILPQEDVETIKN 61 +++WGGRF G + ++EE ASIGFDK L +D++GS+AH TML GI+ ED + I + Sbjct: 12 NQMWGGRFASGPDAIMEEINASIGFDKKLYAQDIRGSIAHATMLAHQGIISAEDKDKIVH 71 Query: 62 GLNKVLGKVRAGEIVFSVADEDIHMNIEKNLIEEIGPVGGKLHTGRSRNDQVATDMHLYL 121 GL+ +L ++ +G FS EDIHMNIE L IGP G+LHT RSRNDQVA D L++ Sbjct: 72 GLDTILSEIDSGAFEFSRRLEDIHMNIEARLAALIGPAAGRLHTARSRNDQVALDFRLWV 131 Query: 122 RNRVVELVALLHELQEALIGQAKDNVDTIVPGYTHLQRAQPILFAHHLLAYVSMFRRDAE 181 + + + L L A + +A+++ +T++PG+THLQ AQP+ F HH +AYV MF RD Sbjct: 132 KEELQKTEKALTGLIAAFLDRAEEHAETVMPGFTHLQTAQPVTFGHHCMAYVEMFGRDRA 191 Query: 182 RLTDSYKRINVLPLGAGALAGTTFPIDRHFVAEQLGFDGVYENSLDAVSDRDFIVEFLAN 241 R+ + + ++ P+GA ALAGT FPIDRH A+ LGF NS+D VSDRDF +EFL+ Sbjct: 192 RVRHAIEHMDESPIGAAALAGTGFPIDRHMTAKALGFREPTRNSIDTVSDRDFALEFLSI 251 Query: 242 AALVMTHLSRLSEELVLWSSTEFSFVELDDAFCTGSSIMPQKKNPDVPELVRGKTGRVYG 301 AA+ THLSRL+EE+V+WS+ +F F+ L DAF TGSSIMPQKKNPD ELVR KTGRV G Sbjct: 252 AAIAATHLSRLAEEIVIWSTPQFGFIRLSDAFSTGSSIMPQKKNPDAAELVRAKTGRVNG 311 Query: 302 NLIGLLTVLKSLPLAYNKDMQEDKEGMFDTVATLTGALQLFAPMISTMKVNKGRMREAVN 361 +L+ LLTV+K LPLAY+KDMQEDKE +FD +L A+ MI + + RM+ A Sbjct: 312 SLVALLTVMKGLPLAYSKDMQEDKEQVFDAAESLELAIAAMTGMIRDVTIRADRMKAAAG 371 Query: 362 TDFSNATDIADFLVGK-GLPFRQAHEVIGKTVLYCINEGKFLLDLTLEEFKQFSPLFDEQ 420 + +S ATD+AD+LV + GLPFR AH V G+ V +G L DL L E + + ++ Sbjct: 372 SGYSTATDLADWLVREAGLPFRDAHHVTGRVVALAEQKGCDLADLPLAELQAINSAITDK 431 Query: 421 IYAVLQPEAVVNARNVYGGTATVQVKAAI 449 +Y VL EA V +R +GGTA +V+ I Sbjct: 432 VYGVLTVEASVASRTSFGGTAPSEVRKQI 460 >SPBC1539.03c [E] COG0165 Argininosuccinate lyase Length = 460 Score = 413 bits (1061), Expect = e-115, Method: Compositional matrix adjust. Identities = 203/455 (44%), Positives = 302/455 (66%), Gaps = 2/455 (0%) Query: 3 KLWGGRFTKGTNKLVEEYTASIGFDKVLAEEDVQGSLAHVTMLGKCGILPQEDVETIKNG 62 KLWGGRFT T+ L+ Y SI +DK + D+ GS A+ L + GI+ ++++ + +G Sbjct: 6 KLWGGRFTGATDPLMTAYNESIHYDKRMFNADIDGSKAYAKALEQRGIISADELDKMLDG 65 Query: 63 LNKVLGKVRAGEIVFSVADEDIHMNIEKNLIEEIGP-VGGKLHTGRSRNDQVATDMHLYL 121 L V + + + +DEDIH E+ L E IG + GKLHTGRSRNDQV TDM L+L Sbjct: 66 LEAVRDEWKTNKFTLQPSDEDIHTANERRLGEIIGTGIAGKLHTGRSRNDQVTTDMRLWL 125 Query: 122 RNRVVELVALLHELQEALIGQAKDNVDTIVPGYTHLQRAQPILFAHHLLAYVSMFRRDAE 181 R+ + + L L + + +A+ ++D I+PGYTHLQRAQPI ++H LL++ D + Sbjct: 126 RDELDIIQKSLVGLLQVFVARAESDLDIIMPGYTHLQRAQPIRWSHFLLSHAFSILNDLD 185 Query: 182 RLTDSYKRINVLPLGAGALAGTTFPIDRHFVAEQLGFDGVYENSLDAVSDRDFIVEFLAN 241 RL Y R+N LPLGAGALAG F +DR F+ ++LGF+GV NS++AV+DRD+++EF+ Sbjct: 186 RLKQIYSRVNRLPLGAGALAGNPFAVDRKFLQKELGFEGVIMNSMNAVADRDYVIEFMFW 245 Query: 242 AALVMTHLSRLSEELVLWSSTEFSFVELDDAFCTGSSIMPQKKNPDVPELVRGKTGRVYG 301 A++VMTH+SRL+E+L+++S++EF+FV L DA+ TGSSIMPQKKNPD EL+RGK+GRV+G Sbjct: 246 ASMVMTHISRLAEDLIIYSTSEFNFVTLSDAYSTGSSIMPQKKNPDSLELLRGKSGRVFG 305 Query: 302 NLIGLLTVLKSLPLAYNKDMQEDKEGMFDTVATLTGALQLFAPMISTMKVNKGRMREAVN 361 N++G + +K +P YNKD+QEDKE +FD+ T+ ++Q+ ++ST+ VN + +++ Sbjct: 306 NMMGFMVSVKGIPSTYNKDLQEDKEPLFDSSKTVLDSIQILTGVLSTLTVNPENIAKSLT 365 Query: 362 TDFSNATDIADFLVGKGLPFRQAHEVIGKTVLYCINEGKFLLDLTLEEFKQFSPLFDEQI 421 D ATD+A++LV KG+PFR+ H + G V + L++E+FK PL++E + Sbjct: 366 ADML-ATDLAEYLVRKGVPFRETHHISGSAVRMAEERNTTIDKLSVEDFKSLHPLYEEDV 424 Query: 422 YAVLQPEAVVNARNVYGGTATVQVKAAIERAEAAL 456 V E+ V R GGTA V I+ + AL Sbjct: 425 ADVFNYESSVEKRCAIGGTAKSCVLEQIQTIKKAL 459 >SPBC1773.14 [E] COG0165 Argininosuccinate lyase Length = 461 Score = 411 bits (1057), Expect = e-114, Method: Compositional matrix adjust. Identities = 202/454 (44%), Positives = 295/454 (64%), Gaps = 1/454 (0%) Query: 3 KLWGGRFTKGTNKLVEEYTASIGFDKVLAEEDVQGSLAHVTMLGKCGILPQEDVETIKNG 62 KLWGGRF+ T+ L+ E+ SI K + EEDV GS+A+ L + ++ +E++ +I G Sbjct: 8 KLWGGRFSGATDPLMAEFNKSIYSGKEMCEEDVIGSMAYAKALCQKNVISEEELNSILKG 67 Query: 63 LNKVLGKVRAGEIVFSVADEDIHMNIEKNLIEEIGPVGGKLHTGRSRNDQVATDMHLYLR 122 L ++ + +G+ V +DED+H E+ L E IG V GKLHTGRSRNDQV TD+ L+L Sbjct: 68 LEQIQREWNSGQFVLEPSDEDVHTANERRLTEIIGDVAGKLHTGRSRNDQVTTDLRLWLC 127 Query: 123 NRVVELVALLHELQEALIGQAKDNVDTIVPGYTHLQRAQPILFAHHLLAYVSMFRRDAER 182 ++ E+ + L + +A+ +D I+ GYTHLQRAQP+ ++H L+++ D R Sbjct: 128 RKIKEVEVYVINLLKVFTNRAEMEIDVIMSGYTHLQRAQPVRWSHFLMSHALPLLGDLGR 187 Query: 183 LTDSYKRINVLPLGAGALAGTTFPIDRHFVAEQLGFDGVYENSLDAVSDRDFIVEFLANA 242 L Y R++ LPLGAGALAG F +DR F+ ++LGF+G+ NS++AV DRDF++EF+ A Sbjct: 188 LRQLYTRVSQLPLGAGALAGNPFNVDREFLRKELGFEGIIMNSMNAVGDRDFVIEFMFWA 247 Query: 243 ALVMTHLSRLSEELVLWSSTEFSFVELDDAFCTGSSIMPQKKNPDVPELVRGKTGRVYGN 302 +VM H+SR +E+L+++SS+EF FV L DA+ TGSSIMPQKKNPD EL+RGK+GRV G+ Sbjct: 248 GMVMLHISRFAEDLIIYSSSEFGFVTLSDAYSTGSSIMPQKKNPDSLELLRGKSGRVLGD 307 Query: 303 LIGLLTVLKSLPLAYNKDMQEDKEGMFDTVATLTGALQLFAPMISTMKVNKGRMREAVNT 362 +IGL+ +K P YNKD+QEDKE +FD T++ +LQ+ ++ST+ +N ++ E++ Sbjct: 308 MIGLMITVKGTPTTYNKDLQEDKEPLFDAFKTVSDSLQILTGVVSTLTINPTKIAESLTP 367 Query: 363 DFSNATDIADFLVGKGLPFRQAHEVIGKTVLYCINEGKFLLDLTLEEFKQFSPLFDEQIY 422 D ATD+A++LV KGLPFRQ H + G V L L++ + + PLFDE + Sbjct: 368 DLL-ATDLAEYLVRKGLPFRQTHHISGSAVRMAEERNTTLDKLSVSDLQSLHPLFDEDVS 426 Query: 423 AVLQPEAVVNARNVYGGTATVQVKAAIERAEAAL 456 V E V R GGTA V+ IE +A+ Sbjct: 427 KVFNYEESVEKRCSIGGTAKHCVQEQIEHIRSAI 460 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,046,857 Number of extensions: 1507804 Number of successful extensions: 3865 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 3842 Number of HSP's successfully gapped: 28 Length of query: 471 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 361 Effective length of database: 25,691,691 Effective search space: 9274700451 Effective search space used: 9274700451 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)