BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0515.0001.p (261 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc3637 [R] COG0627 Predicted esterase 69 6e-12 ZyaiM [R] COG0627 Predicted esterase 62 1e-09 yaiM [R] COG0627 Predicted esterase 62 1e-09 Cgl2815_1 [R] COG0627 Predicted esterase 58 1e-08 Cgl2817 [R] COG0627 Predicted esterase 55 9e-08 >AGc3637 [R] COG0627 Predicted esterase Length = 301 Score = 69.3 bits (168), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 8/120 (6%), Positives = 24/120 (20%), Gaps = 10/120 (8%) Query: 43 PTLYLLHGLSDDDSIWLRRTSIERYV-------AQMGIAVVMPQVHRSFYTDMAE---GG 92 ++ + + +R + + + + + R D Sbjct: 54 GAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLR 113 Query: 93 RYWTFISEELPALARSFFPLSPKREDTFVAGLSMGGYGAVKLGLRKPDTFAAAASLSGAL 152 + + + F+A + AA + L Sbjct: 114 EGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHL 173 >ZyaiM [R] COG0627 Predicted esterase Length = 277 Score = 61.6 bits (148), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 44/165 (26%), Positives = 73/165 (44%), Gaps = 35/165 (21%) Query: 7 KFYSDVLGLSTSMTVILPQQTTTQIGMSNVTKGELHPTLYLLHGLSDDDSIWLRRTSIER 66 + YS L ++ V LP + E P LY L GL+ ++ ++ ++ ++R Sbjct: 18 RHYSQSLKCEMNVGVYLPPKAAN----------EKLPVLYWLSGLTCNEQNFITKSGMQR 67 Query: 67 YVAQMGIAVVMP-------QVHRSFYTDMAEGG---------------RYWTFISEELPA 104 Y A+ I VV P V + D+ +G + + +I ELP Sbjct: 68 YAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPD 127 Query: 105 LARSFFPLSPKREDTFVAGLSMGGYGAVKLGLRKPDTFAAAASLS 149 L FP + K+ ++G SMGG GA+ L LR PD + + ++ S Sbjct: 128 LVMHHFPATAKKS---ISGHSMGGLGALVLALRNPDEYVSVSAFS 169 >yaiM [R] COG0627 Predicted esterase Length = 277 Score = 61.6 bits (148), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 44/165 (26%), Positives = 73/165 (44%), Gaps = 35/165 (21%) Query: 7 KFYSDVLGLSTSMTVILPQQTTTQIGMSNVTKGELHPTLYLLHGLSDDDSIWLRRTSIER 66 + YS L ++ V LP + E P LY L GL+ ++ ++ ++ ++R Sbjct: 18 RHYSQSLKCEMNVGVYLPPKAAN----------EKLPVLYWLSGLTCNEQNFITKSGMQR 67 Query: 67 YVAQMGIAVVMP-------QVHRSFYTDMAEGG---------------RYWTFISEELPA 104 Y A+ I VV P V + D+ +G + + +I ELP Sbjct: 68 YAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPD 127 Query: 105 LARSFFPLSPKREDTFVAGLSMGGYGAVKLGLRKPDTFAAAASLS 149 L FP + K+ ++G SMGG GA+ L LR PD + + ++ S Sbjct: 128 LVMHHFPATAKKS---ISGHSMGGLGALVLALRNPDEYVSVSAFS 169 >Cgl2815_1 [R] COG0627 Predicted esterase Length = 402 Score = 58.2 bits (139), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 67/243 (27%), Positives = 94/243 (38%), Gaps = 49/243 (20%) Query: 43 PTLYLLHGLS--DDDSIWLRRTSIERYVAQMGIAVVMP-QVHRSFYTDMA---EGGRY-W 95 P ++ L GL ++ S W T+IE+Y A VV+P SFY+D G Y W Sbjct: 139 PEIWALDGLRAIEEQSGWTIETNIEQYYADKNAIVVLPVGGESSFYSDWEGPNNGKNYQW 198 Query: 96 -TFISEEL-PALARSFFPLSPKREDTFVAGLSMGGYGAVKLGLRKPDTFAAAASLSGALD 153 TF+++EL P L + F D + G+SMGG AV + PD F S SG LD Sbjct: 199 ETFLTQELAPILDKGFR----SNTDRAITGISMGGTAAVNIATHHPDMFKFVGSFSGYLD 254 Query: 154 MAHHFMNPEDPAR--------KSPEYERVFGKQSIENTPDDLLYLLKEVDRSKGPKPLLY 205 M A + + V ++ EN P VD+ KG + Sbjct: 255 TTSAGMPIAISAALADAGGYDANAMWGPVGSERWQENDPK------SNVDKLKGKTIYVS 308 Query: 206 QCCGTEDFLYEN---------------------NQTFRKACAKTSLSLTYEEGP-GEHEW 243 G +DF E +QTF ++ + + P G H W Sbjct: 309 SGNGADDFGKEGSVAIGPANAAGVGLEVISRMTSQTFVDRASQAGVEVVASFRPSGVHSW 368 Query: 244 GYW 246 YW Sbjct: 369 EYW 371 >Cgl2817 [R] COG0627 Predicted esterase Length = 341 Score = 55.5 bits (132), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 43/122 (35%), Positives = 59/122 (48%), Gaps = 15/122 (12%) Query: 45 LYLLHGL--SDDDSIWLRRTSIERYVAQMGIAVVMP-QVHRSFYTDMAEGGR-------- 93 LYLL G+ ++ + WL T+ R A I +VMP SFY D Sbjct: 89 LYLLDGMRATEYSNAWLVDTNAARLYAPNNITLVMPVGGAGSFYADWNSQASLSSSDPVI 148 Query: 94 -YW-TFISEELPALARSFFPLSPKREDTFVAGLSMGGYGAVKLGLRKPDTFAAAASLSGA 151 W TF+++ELPA F ++ R + + GLSMGG A+ L + PD F A S SG Sbjct: 149 YMWETFLTQELPAYLEQNFGVA--RNNNSIGGLSMGGTAALNLAAKHPDQFRQAMSWSGY 206 Query: 152 LD 153 L+ Sbjct: 207 LN 208 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,630,845 Number of extensions: 859299 Number of successful extensions: 1328 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 1333 Number of HSP's successfully gapped: 21 Length of query: 261 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 157 Effective length of database: 26,383,695 Effective search space: 4142240115 Effective search space used: 4142240115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)