BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0019.p (489 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1894 [G] COG0366 Glycosidases 361 1e-99 AGpT251 [G] COG0366 Glycosidases 303 4e-82 ZycjMm [G] COG0366 Glycosidases 157 5e-38 ycjM [G] COG0366 Glycosidases 155 2e-37 ZycjMm [G] COG0366 Glycosidases 155 2e-37 >SP1894 [G] COG0366 Glycosidases Length = 480 Score = 361 bits (927), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 193/490 (39%), Positives = 284/490 (57%), Gaps = 13/490 (2%) Query: 1 MAIHNQVQLITYPDSLGGSLQTLNHVLDEHFSDIFKGGIHILPPFPSSGDRGFAPLTYFE 60 M I N+ LITY DSLG +L+ L L+EHF D GG+H+LP FPS+ DRGFAP+ Y E Sbjct: 1 MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAI-GGVHLLPFFPSTVDRGFAPVDYDE 59 Query: 61 IEPAFGAWEDIRAIGAKHDVLLDVMVNHISRQSEYFQDFLRQGRRSPYADLFLTLDKIWA 120 ++ AFG WED++ +G K+ ++ D M+NHISRQS+Y++D+ + S + LFL DK W Sbjct: 60 VDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKHEASEFKALFLNWDKFWP 119 Query: 121 GGQPVQADIDKMFLRRPLPYSTFTIEETGEEEKVWTTFGKTDPSEQIDLDIHSDKVKRLL 180 +P Q+D+D ++ R+ + E G E +W TFG+ EQIDLD+ + + Sbjct: 120 ENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGE----EQIDLDVTKEVTMEFI 175 Query: 181 ADIFENFRNNNVKIVRLDAVGYIIKKLGTSCFFVEPEIYGFLDWIKEVTDSLDIELLPEV 240 ++ +N ++RLDA Y +KKL T+ FFVEP+I+ LD ++++ ELLPE+ Sbjct: 176 RKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLDKVRDIAAEYGTELLPEI 235 Query: 241 HSHYSIQYKLAEHGFWIYDFILPYRILDTLVNKNAEALKEYLTTRPAKQFTMLDCHDGIP 300 H HYSIQ+K+A+H +++YDF LP L TL + E L ++L P KQFT LD HDGI Sbjct: 236 HEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKWLKMSPMKQFTTLDTHDGIG 295 Query: 301 VKPDLDGLIDTKEARRLVDTCLERGSNLSLIVSDEHKAE-DGFDVHQVRCSYYSVLNSDD 359 V D+ ++ +E + + G+N+ S AE + D++Q+ +YYS L DD Sbjct: 296 V-VDVKDILTDEEIDYASNELYKVGANVKRKYS---SAEYNNLDIYQINSTYYSALGDDD 351 Query: 360 DAYLAARAIQFFAPGIPQVYYVGLLAGENDXXXXXXXXXXXXINRHNFSIDEIEQALGKS 419 Y AR IQ FAPGIPQ+YYVGLLAG+ND INRH +S +EI + + + Sbjct: 352 VKYFLARLIQAFAPGIPQIYYVGLLAGKNDLKLLEETKEGRNINRHYYSNEEIAKEVQRP 411 Query: 420 AVQRLLALIRFRNEYGAFN--GDFRVLEAASNEVRLEWTKGDLRCTLYIDLSTNRTVIDY 477 V+ LL L FRN AF+ G + ++ + ++ D T +++ Sbjct: 412 VVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQNKDKSVTAVVEIDLQNQTYRV 471 Query: 478 VETEGMSVQF 487 +E G+ V F Sbjct: 472 IEN-GVEVSF 480 >AGpT251 [G] COG0366 Glycosidases Length = 35 Score = 303 bits (776), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 0/462 (0%), Positives = 0/462 (0%), Gaps = 19/462 (4%) Query: 23 LNHVLDEHFSDIFKGGIHILPPF-PSSG-DRGFAPLTYFEIEPAFGAWEDIRAIGAKHDV 80 + ++ S + L + Sbjct: 1 MMMLIGRLSSRAG-CRLKPLTSIVKLTWR 59 Query: 81 LLDVMVNHISRQSEYFQDFLRQGRRSPYADLFLTLDKIWAGGQPVQADIDKMFLRRP-LP 139 Sbjct: 60 - 118 Query: 140 YSTFTIEETGEEEKVWTTFGKTDPSEQIDLDIHSDKVKRLLADIFENFRNNNVKIVRLDA 199 Sbjct: 119 ----- 173 Query: 200 VGYIIKKLGTSCFFVEPEIYGFLDWIKEVTDSLDIELLPEVHSHYSIQYKLAEHGFWIYD 259 Sbjct: 174 - 232 Query: 260 FILPYRILDTLVNKNAEALKEYLTTRPAKQFTMLDCHDGIPV------KPDLDGLIDTKE 313 Sbjct: 233 292 Query: 314 ARRLVDTCLERGSNLSLIVSDEHKAEDGFDVHQVRCSYYSVLNSDDDAYLAARAIQFFAP 373 Sbjct: 293 -- 350 Query: 374 GIPQVYYVGLLAGENDXXXXXXXXXXXXINRHNFSIDEIEQALGKSAVQRLLALIRFRNE 433 Sbjct: 351 410 Query: 434 YGAFNGDFRVLEAASNEVRLEWTKGDLRCTLYIDLSTNRTVI 475 Sbjct: 411 452 >ZycjMm [G] COG0366 Glycosidases Length = 568 Score = 157 bits (396), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 25/509 (4%), Positives = 80/509 (15%), Gaps = 57/509 (11%) Query: 7 VQLITYPDSLGGS----LQTLNHVLDEHFSDIFKGGIHILPPFPSSGDRGFAPLTYFEIE 62 + + N + + F + F F P + + Sbjct: 49 RKKHWDESDVVLITYADQFHSNDLKPLPTFNQF-YHQWLQSIFSHVHLLPFYPWSSDDGF 107 Query: 63 PAFGAWEDIRAIGAKHDVLLDVMVNHISRQSEYFQDFLRQGRRSPYADLFLTLDKIWAGG 122 + G D+ +H+ + L Sbjct: 108 SVIDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKS--EWFKNYLQQHPGF----- 160 Query: 123 QPVQADIDKMFLRRPLPYSTFTIEETGEEEKVWTTFGKTDPSEQIDLDIHSDKVKRLLAD 182 + + + L T + + D++ Sbjct: 161 ---EDFFIAVDPQTDLSAVTRPRALPLLTPFQMRDHS----TRHLWTTFSDDQIDLNYRS 213 Query: 183 IFENFRNNNVKIVRLDAVGYIIKKLGTSCFFVEPEIYGFLDWIKEVTDSLDIELLPEVHS 242 +V + L ++ + E + K + + + + Sbjct: 214 PEVLLAMVDVLLCYLAKGAEYVRLDAVGFMWKE-PGTSCIHLEKTHLIIKLLRSIIDNVA 272 Query: 243 HYSIQYKLAEHG------FWIYDFILPYRILDTLVNKNAEALKEY-----LTTRPAKQFT 291 ++ ++ + + + + L Sbjct: 273 PGTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTL 332 Query: 292 MLDCHDGIPVKPDLDGLIDTKEARRLVDTCLERGSNLSLIVSDEHKAEDGFDVHQVRCSY 351 I R L+ + + + + + + Sbjct: 333 PSSNTTWFNFLA-SHDGIGLNPLRGLLPESEILEL----VEALQQEGALVNWKNNPDGTR 387 Query: 352 YSVLNSDDDA-------YLAARAIQFFAPGIPQVYYVGLLAGENDXXXXXXXXXXXXINR 404 + F + + + + Sbjct: 388 SPYEINVTYMDALSRRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEK 447 Query: 405 HNFSIDEIEQALGKSA---------VQRLLALIRFRNEYGAFNGDFRVLEAASNEVRLEW 455 ++ + R +N Sbjct: 448 LGYNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLRR-SHNEFHPDNNFTIDTI 506 Query: 456 TKGDLRC-TLYIDLSTNRTVIDYVETEGM 483 +R D + Sbjct: 507 NSSVMRIPRSNADGNCL---TGLFNVSKN 532 >ycjM [G] COG0366 Glycosidases Length = 568 Score = 155 bits (391), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 132/515 (25%), Positives = 228/515 (44%), Gaps = 54/515 (10%) Query: 7 VQLITYPDSLGGS----LQTLNHVLDEHFSDIFKGGIHILPPFPSSGDRGFAPLTYFEIE 62 V LITY D + L T N + IF +H+LP +P S D GF+ + Y ++ Sbjct: 58 VVLITYADQFHSNDLKPLPTFNQFYHQWLQSIF-SHVHLLPFYPWSSDDGFSVIDYHQVA 116 Query: 63 PAFGAWEDIRAIGAKHDVLLDVMVNHISRQSEYFQDFLRQGRRSPYADLFLTLDKIWAGG 122 G W+DI+ +G ++ D + NH+S +SE+F+++L+Q + D F+ +D Sbjct: 117 SEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQ--HPGFEDFFIAVDP----- 169 Query: 123 QPVQADIDKMFLRRPLPYSTFTIEETGEEEKVWTTFGKTDPSEQIDLDIHSDKVKRLLAD 182 Q D+ + R LP T +WTTF +QIDL+ S +V + D Sbjct: 170 ---QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFS----DDQIDLNYRSPEVLLAMVD 222 Query: 183 IFENFRNNNVKIVRLDAVGYIIKKLGTSCFFVEPEIYGFLDWIKEVTDSLDIELLPEVHS 242 + + + VRLDAVG++ K+ GTSC +E + + + ++ + D++ + + Sbjct: 223 VLLCYLAKGAEYVRLDAVGFMWKEPGTSCIHLE-KTHLIIKLLRSIIDNVAPGTVIITET 281 Query: 243 HYSIQYKLAEHG------FWIYDFILPYRILDTLVNKNAEALKEY-----LTTRPAKQFT 291 + + +A G +Y F LP +L + +N EAL + L + F Sbjct: 282 NVPHKDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFN 341 Query: 292 MLDCHDGIPVKPDLDGLIDTKEARRLVDTCLERGSNLSLIVSDEHKAEDGFDVHQVRCSY 351 L HDGI + P L GL+ E LV+ + G+ +V+ ++ + +++ +Y Sbjct: 342 FLASHDGIGLNP-LRGLLPESEILELVEALQQEGA----LVNWKNNPDGTRSPYEINVTY 396 Query: 352 YSVLNSDDDA-------YLAARAIQFFAPGIPQVYYVGLLAGENDXXXXXXXXXXXXINR 404 L+ + + ++ A AI PG+P +Y +L ND INR Sbjct: 397 MDALSRRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINR 456 Query: 405 HNFSIDEIEQALGKSA---------VQRLLALIRFRNEYGAFNGDFRVLEAASNEVRLEW 455 + EI + L A + RL+ L R NE+ N +F + S+ +R+ Sbjct: 457 KKYHSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDN-NFTIDTINSSVMRIPR 515 Query: 456 TKGDLRC-TLYIDLSTNRTVIDYVETEGMSVQFEV 489 + D C T ++S N ++ G + EV Sbjct: 516 SNADGNCLTGLFNVSKNIQHVNITNLHGRDLISEV 550 Score = 155 bits (391), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 132/515 (25%), Positives = 228/515 (44%), Gaps = 54/515 (10%) Query: 7 VQLITYPDSLGGS----LQTLNHVLDEHFSDIFKGGIHILPPFPSSGDRGFAPLTYFEIE 62 V LITY D + L T N + IF +H+LP +P S D GF+ + Y ++ Sbjct: 58 VVLITYADQFHSNDLKPLPTFNQFYHQWLQSIF-SHVHLLPFYPWSSDDGFSVIDYHQVA 116 Query: 63 PAFGAWEDIRAIGAKHDVLLDVMVNHISRQSEYFQDFLRQGRRSPYADLFLTLDKIWAGG 122 G W+DI+ +G ++ D + NH+S +SE+F+++L+Q + D F+ +D Sbjct: 117 SEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQ--HPGFEDFFIAVDP----- 169 Query: 123 QPVQADIDKMFLRRPLPYSTFTIEETGEEEKVWTTFGKTDPSEQIDLDIHSDKVKRLLAD 182 Q D+ + R LP T +WTTF +QIDL+ S +V + D Sbjct: 170 ---QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFS----DDQIDLNYRSPEVLLAMVD 222 Query: 183 IFENFRNNNVKIVRLDAVGYIIKKLGTSCFFVEPEIYGFLDWIKEVTDSLDIELLPEVHS 242 + + + VRLDAVG++ K+ GTSC +E + + + ++ + D++ + + Sbjct: 223 VLLCYLAKGAEYVRLDAVGFMWKEPGTSCIHLE-KTHLIIKLLRSIIDNVAPGTVIITET 281 Query: 243 HYSIQYKLAEHG------FWIYDFILPYRILDTLVNKNAEALKEY-----LTTRPAKQFT 291 + + +A G +Y F LP +L + +N EAL + L + F Sbjct: 282 NVPHKDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFN 341 Query: 292 MLDCHDGIPVKPDLDGLIDTKEARRLVDTCLERGSNLSLIVSDEHKAEDGFDVHQVRCSY 351 L HDGI + P L GL+ E LV+ + G+ +V+ ++ + +++ +Y Sbjct: 342 FLASHDGIGLNP-LRGLLPESEILELVEALQQEGA----LVNWKNNPDGTRSPYEINVTY 396 Query: 352 YSVLNSDDDA-------YLAARAIQFFAPGIPQVYYVGLLAGENDXXXXXXXXXXXXINR 404 L+ + + ++ A AI PG+P +Y +L ND INR Sbjct: 397 MDALSRRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINR 456 Query: 405 HNFSIDEIEQALGKSA---------VQRLLALIRFRNEYGAFNGDFRVLEAASNEVRLEW 455 + EI + L A + RL+ L R NE+ N +F + S+ +R+ Sbjct: 457 KKYHSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDN-NFTIDTINSSVMRIPR 515 Query: 456 TKGDLRC-TLYIDLSTNRTVIDYVETEGMSVQFEV 489 + D C T ++S N ++ G + EV Sbjct: 516 SNADGNCLTGLFNVSKNIQHVNITNLHGRDLISEV 550 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.140 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,125,488 Number of extensions: 1591168 Number of successful extensions: 6680 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 6689 Number of HSP's successfully gapped: 12 Length of query: 489 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 379 Effective length of database: 25,691,691 Effective search space: 9737150889 Effective search space used: 9737150889 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)