BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0011.p (296 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1742 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneurami... 183 3e-46 BS_dapA [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuram... 164 2e-40 BMEI1301 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneura... 152 7e-37 yjhH [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuramina... 150 2e-36 BH2399 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneurami... 150 2e-36 >BH1742 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 295 Score = 183 bits (464), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 102/285 (35%), Positives = 161/285 (56%), Gaps = 3/285 (1%) Query: 2 LEVKGIIPAMVTPLDQREEIDEKALRQMVNRYIDAGVHGLFCLGTNGEFFNLSFEEKLTV 61 + + I+ AMVTP +++++++ +A Q+VN I G L GT GE L+ +EK+ + Sbjct: 1 MNIGRIVTAMVTPFNEQDQVNLEATCQLVNELIANGSDALVVGGTTGESPTLTTDEKMAL 60 Query: 62 AQIVIHEARGRVPVYAGAGCAGTGETRRLAQRLEQAGADALSVITPYFLTYTQPELQYHF 121 V+ A GRVPV AG G T + L++++E G D + ++TPY+ +Q L HF Sbjct: 61 FHTVVEAAGGRVPVIAGTGSNNTRASIELSKKVEDLGVDGIMLVTPYYNKPSQEGLFQHF 120 Query: 122 EAVAGETSLPLVLYTIPARTGNTLSVQTVAKLAELPNVVGIKDSSGSFDNILQLIDHTPE 181 +A++ TSLP++LY IP R+G L +T +LAEL N+V IK++SG+ D I ++I+H+P Sbjct: 121 KAISESTSLPIMLYNIPGRSGVNLDAETTLRLAELRNIVSIKEASGNLDQIAEIIEHSPA 180 Query: 182 SFSVLAGTDSLILSTLMXXXXXXXXXXXNIFPSTVVAIYEHWQCGEYEQAEQAQRVLRDL 241 FSV +G DSL L L +I + + + + + G+ E+A R L L Sbjct: 181 DFSVYSGDDSLTLPLLSIGGTGVVSVASHIIGNEMQEMVQLFLSGQVERAAAIHRKLLPL 240 Query: 242 RAAFALGTLPSVLKAVLDEIGLPAGPARRPVAPLTPQALEEVRRM 286 + P+ +KA L+ GLP G R P+ PLT EE R++ Sbjct: 241 MKTLFMTPNPTAVKAALELKGLPVGHVRLPLVPLTA---EEQRQL 282 >BS_dapA [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 290 Score = 164 bits (415), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 91/268 (33%), Positives = 146/268 (54%), Gaps = 1/268 (0%) Query: 10 AMVTPLDQREEIDEKALRQMVNRYIDAGVHGLFCLGTNGEFFNLSFEEKLTVAQIVIHEA 69 AM+TP D + +D + L +++ + G L GT GE LS EEK+ + + + E Sbjct: 9 AMITPFDNKGNVDFQKLSTLIDYLLKNGTDSLVVAGTTGESPTLSTEEKIALFEYTVKEV 68 Query: 70 RGRVPVYAGAGCAGTGETRRLAQRLEQAGADALSVITPYFLTYTQPELQYHFEAVAGETS 129 GRVPV AG G T ++ +L ++ E+AG DA+ ++TPY+ +Q + HF+A+A ETS Sbjct: 69 NGRVPVIAGTGSNNTKDSIKLTKKAEEAGVDAVMLVTPYYNKPSQEGMYQHFKAIAAETS 128 Query: 130 LPLVLYTIPARTGNTLSVQTVAKL-AELPNVVGIKDSSGSFDNILQLIDHTPESFSVLAG 188 LP++LY +P RT +L+ +T +L A++PNVV IK++SG + I ++I TPE F V +G Sbjct: 129 LPVMLYNVPGRTVASLAPETTIRLAADIPNVVAIKEASGDLEAITKIIAETPEDFYVYSG 188 Query: 189 TDSLILSTLMXXXXXXXXXXXNIFPSTVVAIYEHWQCGEYEQAEQAQRVLRDLRAAFALG 248 D+L L L +I + + + +++ G+ A + L + Sbjct: 189 DDALTLPILSVGGRGVVSVASHIAGTDMQQMIKNYTNGQTANAALIHQKLLPIMKELFKA 248 Query: 249 TLPSVLKAVLDEIGLPAGPARRPVAPLT 276 P+ +K L GL G R P+ PLT Sbjct: 249 PNPAPVKTALQLRGLDVGSVRLPLVPLT 276 >BMEI1301 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 307 Score = 152 bits (383), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 100/281 (35%), Positives = 138/281 (49%), Gaps = 1/281 (0%) Query: 4 VKGIIPAMVTPLDQREEIDEKALRQMVNRYIDAGVHGLFCLGTNGEFFNLSFEEKLTVAQ 63 +KG I A+VTP D+ DEKA R VN I+ G GL +GT GE LS +E V + Sbjct: 16 LKGSITALVTPFDREGAFDEKAFRAFVNWQIEEGTKGLVPVGTTGETPTLSHDEHKRVIE 75 Query: 64 IVIHEARGRVPVYAGAGCAGTGETRRLAQRLEQAGADALSVITPYFLTYTQPELQYHFEA 123 + I A GRVPV AGAG T E LAQ E+AGADA+ V+TPY+ Q L HF Sbjct: 76 VCIEVAAGRVPVIAGAGSNNTVEAIELAQHAEKAGADAVLVVTPYYNKPNQRGLYEHFSR 135 Query: 124 VAGETSLPLVLYTIPARTGNTLSVQTVAKLA-ELPNVVGIKDSSGSFDNILQLIDHTPES 182 VA S+PLV+Y IP R+ ++ +T+ L + N+VG+KD++G + + + + Sbjct: 136 VARSISIPLVIYNIPGRSIIDMTPETMGALVRDCKNIVGVKDATGKIERVSEQRAICGKE 195 Query: 183 FSVLAGTDSLILSTLMXXXXXXXXXXXNIFPSTVVAIYEHWQCGEYEQAEQAQRVLRDLR 242 F L+G D+ L NI P E Q G + +A + Q L L Sbjct: 196 FIQLSGEDATALGFNAHGGVGCISVTSNIAPRLCAEFQEACQAGNFAKALELQDRLMPLH 255 Query: 243 AAFALGTLPSVLKAVLDEIGLPAGPARRPVAPLTPQALEEV 283 A L PS K L +G R P+ + E++ Sbjct: 256 KALFLEPNPSGPKYALSRLGRIENVLRSPMVTIEAATAEKI 296 >yjhH [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 319 Score = 150 bits (380), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 89/269 (33%), Positives = 139/269 (51%), Gaps = 5/269 (1%) Query: 1 MLEVKGIIPAMVTPLDQREEIDEKALRQMVNRYIDAGVHGLFCLGTNGEFFNLSFEEKLT 60 M + GIIP + + + +D+KA+R++ + I+ GV GLF LGT GEF ++ +++ Sbjct: 19 MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYLGTGGEFSQMNTAQRMA 78 Query: 61 VAQIVIHEARGRVPVYAGAGCAGTGETRRLAQRLEQAGADALSVITPYFLTYTQPELQYH 120 +A+ + GRVPV G G T E +LAQ + GAD + I PY+ L + Sbjct: 79 LAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGIVAINPYYWKVAPRNLDDY 138 Query: 121 FEAVAGETSLPLVLYTIPARTGNTLSVQTVAKLA-ELPNVVGIK---DSSGSFDNILQLI 176 ++ +A +LP++LY P TG L+ +TV +LA + N+VGIK DS G ++ + Sbjct: 139 YQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENIVGIKDTIDSVGHLRTMINTV 198 Query: 177 DHTPESFSVLAGTDSLILSTLMXXXXXXXXXXXNIFPSTVVAIYEHWQCGEYEQAEQAQR 236 SFSV G D +L+T++ N P V IY W+ G+ A + Sbjct: 199 KSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPELSVGIYRAWREGDLATAATLNK 258 Query: 237 VLRDLRAAFALGT-LPSVLKAVLDEIGLP 264 L L A +AL T S++K + +GLP Sbjct: 259 KLLQLPAIYALETPFVSLIKYSMQCVGLP 287 >BH2399 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 292 Score = 150 bits (379), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 84/281 (29%), Positives = 144/281 (51%) Query: 7 IIPAMVTPLDQREEIDEKALRQMVNRYIDAGVHGLFCLGTNGEFFNLSFEEKLTVAQIVI 66 ++ AMVTP D + +D + + ++V + G GL GT GE L EEKL + + V+ Sbjct: 6 VLTAMVTPFDAKGNVDLEKVERLVEFLLANGTEGLVVAGTTGESPTLREEEKLALFEKVV 65 Query: 67 HEARGRVPVYAGAGCAGTGETRRLAQRLEQAGADALSVITPYFLTYTQPELQYHFEAVAG 126 RVPV AG G T + +L ++ G D + +TPY+ +Q + HF A+A Sbjct: 66 TVVNKRVPVIAGTGSNNTYVSAQLTKKATALGVDGIMAVTPYYNKPSQEGMYAHFAAIAE 125 Query: 127 ETSLPLVLYTIPARTGNTLSVQTVAKLAELPNVVGIKDSSGSFDNILQLIDHTPESFSVL 186 T LP++LY IP+R+ LS +T+ KL+ +PN+ +K+++G D+++ +I+ TP++F + Sbjct: 126 ATHLPVMLYNIPSRSVVNLSAETIVKLSYIPNITSLKEANGDLDHMVSVIEQTPDTFHLY 185 Query: 187 AGTDSLILSTLMXXXXXXXXXXXNIFPSTVVAIYEHWQCGEYEQAEQAQRVLRDLRAAFA 246 +G DSL L L +I + + ++ G+ E A R L + Sbjct: 186 SGDDSLTLPALSIGAKGVVSVASHIIGPEMQQMMMAFRGGDVEHAANLHRKLLPIMKGLF 245 Query: 247 LGTLPSVLKAVLDEIGLPAGPARRPVAPLTPQALEEVRRMV 287 P+ +KA L G G R P+ P T + + ++ ++ Sbjct: 246 TAPNPTCVKAALQIKGFDTGGVRLPLVPPTSEQRQHLQFLI 286 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,902,920 Number of extensions: 810358 Number of successful extensions: 4010 Number of sequences better than 1.0e-05: 78 Number of HSP's gapped: 3937 Number of HSP's successfully gapped: 78 Length of query: 296 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 190 Effective length of database: 26,153,027 Effective search space: 4969075130 Effective search space used: 4969075130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)