BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0007.p (385 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGpA199 [C] COG1454 Alcohol dehydrogenase, class IV 197 2e-50 ZyiaY [C] COG1454 Alcohol dehydrogenase, class IV 190 3e-48 ECs4466 [C] COG1454 Alcohol dehydrogenase, class IV 190 3e-48 PA1991 [C] COG1454 Alcohol dehydrogenase, class IV 188 1e-47 STM2973 [C] COG1454 Alcohol dehydrogenase, class IV 187 3e-47 >AGpA199 [C] COG1454 Alcohol dehydrogenase, class IV Length = 435 Score = 197 bits (502), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 21/382 (5%), Positives = 61/382 (15%), Gaps = 2/382 (0%) Query: 4 YFFQTARSIIAGCGSLERIVEHLQKLGSFESVLLVSQPFLQSSGTLDEVTNLLKSGGIAV 63 + ++ + S Q + + Sbjct: 19 KPNLARQGGDMAIAKQIEATIGQKEDAGGKIARHASLLSQQLQELRTRMYPPKSEKSLRP 78 Query: 64 HWNTDVQPEPTIAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVREMLG 123 +V +I + V + ++ + + + Sbjct: 79 FLTNEVSKLTSIPDSTLKLMSSEGRGPVPGRLENNHRVYTLAQINELREFFASQKPSEAL 138 Query: 124 TDLIPNPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXXXG 183 L + + + R Sbjct: 139 RFLPRRREGEHLQVVAIANFKGGSAKTTTCVHLAHY-LALQGYRVLALDLDPQASLSALF 197 Query: 184 LPRDITASTGMDAYTHALESFIS-NKANRFSDMFALESIRNISASMLQAYHHGNDRKARE 242 + + AL + + R ++ L+ + ++ Sbjct: 198 GAQPEFDVGANETIYAALRYDDAERRPIRDIIRKTYFDGIDLIPGNLEVMEYEHETPRVL 257 Query: 243 DMLIGSMYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERLA 302 GS + V + G + V L Sbjct: 258 AQKSGSGAIFFERLKLALSEVEQDYDIVILDTPPSLGFLTLSAIYAATSMIITVHPAMLD 317 Query: 303 LVAGAMGLQTGAGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQVTR 362 + + + L Q+ + + + + + Sbjct: 318 VASMSQFLLMMGDLISVLNESGAQLDQDFIRYLVTRHDPNDAPQSQVVAMMRHLFGSDVL 377 Query: 363 LMGNNPKAMTLDDIGAVYGKLF 384 L A Sbjct: 378 LPTLIESTAVEAAGLAKRSIYE 399 >ZyiaY [C] COG1454 Alcohol dehydrogenase, class IV Length = 382 Score = 190 bits (483), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 123/378 (32%), Positives = 187/378 (49%), Gaps = 6/378 (1%) Query: 5 FFQTARSIIAGCGSLERIVEHLQKLGSFESVLLVSQPFLQSSGTLDEVTNLLKSGGIAVH 64 FF + ++I G SL + + G F L+V+ L G +V L+ I Sbjct: 6 FFIPSVNVI-GADSLTDAMNMMADYG-FTRTLIVTDNMLTKLGMAGDVQKALEERNIFSV 63 Query: 65 WNTDVQPEPTIAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVREMLGT 124 QP PT + + E CD++I +GGGS D K ++++ N +R+ G Sbjct: 64 IYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHDCAKGIALVAANGGDIRDYEGV 123 Query: 125 DLIPNPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXXXGL 184 D P +P + I TT+GT SE+T I+T +K+ +V ++ G+ Sbjct: 124 DRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIKMAIVDKHVTPLLSVNDSSLMIGM 183 Query: 185 PRDITASTGMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKAREDM 244 P+ +TA+TGMDA THA+E+++S A +D AL+++ I+ ++ A G++ KARE M Sbjct: 184 PKSLTAATGMDALTHAIEAYVSIAATPITDACALKAVTMIAENLPLAVEDGSNAKAREAM 243 Query: 245 LIGSMYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERLALV 304 GMA A VHA+A+ +GG +++PHGV N++LLPHV FN V RL Sbjct: 244 AYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPHGVCNAVLLPHVQVFNSKVAAARLRDC 303 Query: 305 AGAMGLQ-TGAGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQVTRL 363 A AMG+ TG AE I IR +++ IP L ED LA + + Sbjct: 304 AAAMGVNVTGKNDAEGAEACINAIRELAKKVDIPAGLRDLNVKEEDFA--VLATNALKDA 361 Query: 364 MG-NNPKAMTLDDIGAVY 380 G NP T ++I A+Y Sbjct: 362 CGFTNPIQATHEEIVAIY 379 >ECs4466 [C] COG1454 Alcohol dehydrogenase, class IV Length = 383 Score = 190 bits (483), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 124/378 (32%), Positives = 188/378 (49%), Gaps = 6/378 (1%) Query: 5 FFQTARSIIAGCGSLERIVEHLQKLGSFESVLLVSQPFLQSSGTLDEVTNLLKSGGIAVH 64 FF + ++I G SL + + G F L+V+ L G +V L+ I Sbjct: 6 FFIPSVNVI-GADSLTDAMNMMADYG-FTRTLIVTDSMLTKLGMAGDVQKALEERNIFSV 63 Query: 65 WNTDVQPEPTIAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVREMLGT 124 QP PT + + E CD++I +GGGS D K ++++ N +R+ G Sbjct: 64 IYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHDCAKGIALVAANGGDIRDYEGV 123 Query: 125 DLIPNPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXXXGL 184 D P +P + I TT+GT SE+T I+T +K+ +V ++ G+ Sbjct: 124 DRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIKMAIVDKHVTPLLSVNDSSLMIGM 183 Query: 185 PRDITASTGMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKAREDM 244 P+ +TA+TGMDA THA+E+++S A +D AL+++ I+ ++ A G++ KARE M Sbjct: 184 PKSLTAATGMDALTHAIEAYVSIAATPITDACALKAVTMIAENLPLAVEDGSNAKAREAM 243 Query: 245 LIGSMYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERLALV 304 GMA A VHA+A+ +GG +++PHGV N++LLPHV FN V RL Sbjct: 244 AYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPHGVCNAVLLPHVQVFNSKVAAARLRDC 303 Query: 305 AGAMGLQ-TGAGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQVTRL 363 A AMG+ TG AE I IR +++ IP L ED LAA+ + Sbjct: 304 AAAMGVNVTGKNDAEGAEACINAIRELAKKVDIPAGLRDLNVKEEDFA--VLAANALKDA 361 Query: 364 MG-NNPKAMTLDDIGAVY 380 G NP T ++I A+Y Sbjct: 362 CGFTNPIQATHEEIVAIY 379 >PA1991 [C] COG1454 Alcohol dehydrogenase, class IV Length = 387 Score = 188 bits (477), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 118/379 (31%), Positives = 196/379 (51%), Gaps = 17/379 (4%) Query: 12 IIAGCGSLERIVEHLQKLGSFESVLLVSQPFLQSSGTLDEVTNLLKSGGIAVHWNTDVQP 71 II G G + +++ G+ VLLVS P + ++G + ++ L+ GI TDV P Sbjct: 16 IIFGAGCRHSVANYVKTFGA-RKVLLVSDPGVLAAGWVADIQASLEGQGIDYCLYTDVSP 74 Query: 72 EPTIAQL---EELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVREMLGTDLIP 128 P + ++ E Y + C+ I+ +GGGS +D K + ++ + S+ E G D + Sbjct: 75 NPRVDEVMLGAEFYRSEG---CNVIVAVGGGSPMDCAKGIGIVAAHGRSILEFEGVDRLR 131 Query: 129 NPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXXXGLPRDI 188 P P +LIPTT+GT ++V+ I++ +E +K VVS+ + + Sbjct: 132 VPSPPLILIPTTAGTSADVSQFVIISNQDERMKFSVVSKAVVPDVSLIDPETTLTMDPFL 191 Query: 189 TASTGMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKAREDMLIGS 248 +A TG+DA HA+E+F+S +D ALE++R I +++Q + D RE +++GS Sbjct: 192 SACTGIDALVHAIEAFVSTGHGPLTDPHALEAMRLIDGNLVQMIANPGDIALREKIMLGS 251 Query: 249 MYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERLALVA--- 305 M G+A + A AVHA+++ +GG +PHG+ N+ L+ HV+ FN+ ER ++A Sbjct: 252 MQAGLAFSNAILGAVHAMSHSLGGYLDLPHGLCNAALVEHVVAFNYSAAPERFKVIAETF 311 Query: 306 --GAMGLQTGAGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQVTRL 363 GL G RQR +H+I + + + L L G DIP LS A Q + Sbjct: 312 GIDCRGLTQGQVRQRLVDHLIA----FKHAVGFHESLALHGVGSSDIPFLSRHAMQDPCI 367 Query: 364 MGNNPKAMTLDDIGAVYGK 382 + NP+ + D+ VYG+ Sbjct: 368 L-TNPRESSQRDVEVVYGE 385 >STM2973 [C] COG1454 Alcohol dehydrogenase, class IV Length = 382 Score = 187 bits (475), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 116/369 (31%), Positives = 189/369 (51%), Gaps = 7/369 (1%) Query: 15 GCGSLERIVEHLQKLGSFESVLLVSQPFLQSSGTLDEVTNLLKSGGIAVHWNTDVQPEPT 74 G G+++ + + + + G + L+V+ L G +D+VT+ + + G+A V P PT Sbjct: 14 GRGAVDALTDEVTRRG-YHKALIVTDKTLVQCGVVDKVTSRMDAAGLAWEIYAGVIPNPT 72 Query: 75 IAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNS--LSVREMLGTDLIPNPGI 132 I+ ++E + D +I IGGGS D K + ++ N VR + G P + Sbjct: 73 ISVVQEGLKVFTQSGADYLIAIGGGSPQDTCKAIGIISNNPEFADVRSLEGLSPTRKPSV 132 Query: 133 PTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXXXGLPRDITAST 192 P + IPTT+GT +EVT N ++T E+ K V + G+P + A+T Sbjct: 133 PIMAIPTTAGTAAEVTINYVITDEEKRRKFVCVDPHDIPQAAFIDADMMDGMPPALKAAT 192 Query: 193 GMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKAREDMLIGSMYGG 252 G+DA THA+E +I+ A +D +++I I+ ++ A +++A E M +G G Sbjct: 193 GVDALTHAIEGYITRAAWALTDALHIKAIEIIAGALRGAV--AGEKEAGEAMALGQYVAG 250 Query: 253 MALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERLALVAGAMGLQT 312 M + G VH +A+P+G ++ PHGVAN++LLPHVM FN E+ +A AMG++ Sbjct: 251 MGFSNVGLGLVHGMAHPLGAFYNTPHGVANAILLPHVMRFNAGSTNEKFRDIARAMGVKV 310 Query: 313 -GAGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQVTRLMGNNPKAM 371 G + +E + R++ IP L G +EDIP L+ AA G NP+ Sbjct: 311 EGLSLEEARNAAVEAVFTLNRDVGIPLHLRDVGVRKEDIPALAQAAFDDV-CTGGNPREA 369 Query: 372 TLDDIGAVY 380 +L DI +Y Sbjct: 370 SLADIVELY 378 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,972,121 Number of extensions: 1043381 Number of successful extensions: 2340 Number of sequences better than 1.0e-05: 67 Number of HSP's gapped: 2178 Number of HSP's successfully gapped: 67 Length of query: 385 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 277 Effective length of database: 25,922,359 Effective search space: 7180493443 Effective search space used: 7180493443 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)