BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0003.p (428 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2057 [S] COG0586 Uncharacterized membrane-associated protein 138 2e-32 BS_ybfM [S] COG0586 Uncharacterized membrane-associated protein 138 2e-32 BS_yngC [S] COG0586 Uncharacterized membrane-associated protein 98 2e-20 all4799 [S] COG0586 Uncharacterized membrane-associated protein 91 4e-18 slr0509 [S] COG0586 Uncharacterized membrane-associated protein 89 1e-17 >BH2057 [S] COG0586 Uncharacterized membrane-associated protein Length = 200 Score = 138 bits (347), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 63/161 (39%), Positives = 108/161 (67%) Query: 1 LDILTHLLEHYGYGLIFLFLCLEMLALPLPGEMLMSYIGLSVYEQKLSWMLCIVSAGTGV 60 +++ LL YGY + L L ++ LP+P E+L++ +G + K+S++L ++++ G Sbjct: 1 MELFEFLLIKYGYIALILILTGGIVGLPIPDEVLLTLVGYYISLGKMSYVLALLASFLGA 60 Query: 61 LLGVTLSYWVGYRLGRPFIARYGARIHLTEERIVKMNRWFEKYGDKLLFVAYFIPGVRHI 120 LG+++SY++G +LG PF+ +YG + H++ ++I F KYG LLF+ YF+PGVRH+ Sbjct: 61 TLGISISYFLGTKLGLPFLMKYGPKFHISHQKIDHAQALFRKYGPILLFIGYFLPGVRHV 120 Query: 121 TGYFCGVTRMPFRRYALFAYSGAIFWVSLFISLGKVLGPKW 161 TGY G+++ FR ++L+AY+GA W +FI+LG +G KW Sbjct: 121 TGYLSGLSKCKFRTFSLYAYTGAFIWCLVFITLGHEIGNKW 161 >BS_ybfM [S] COG0586 Uncharacterized membrane-associated protein Length = 468 Score = 138 bits (347), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 12/158 (7%), Positives = 25/158 (15%) Query: 1 LDILTHLLEHYGYGLIFLFLCLEMLALPLPGEMLMSYIGLSVYEQKLSWMLCIVSAGTGV 60 + + + + +A+P T Sbjct: 1 MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGGIYYWQRERDRKDVTDG 60 Query: 61 LLGVTLSYWVGYRLGRPFIARYGARIHLTEERIVKMNRWFEKYGDKLLFVAYFIPGVRHI 120 T + F + Y A + + E F + Sbjct: 61 DKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAENGDSSHPNEIAFSKSNKAY 120 Query: 121 TGYFCGVTRMPFRRYALFAYSGAIFWVSLFISLGKVLG 158 + G A + W Sbjct: 121 DEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQT 158 >BS_yngC [S] COG0586 Uncharacterized membrane-associated protein Length = 198 Score = 98.2 bits (243), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 56/190 (29%), Positives = 100/190 (52%), Gaps = 5/190 (2%) Query: 2 DILTHLLEHYGYGLIFLFLCLEMLALPLPGEMLMSYIGLSVYEQKLSWMLCIVSAGTGVL 61 +ILT L + GY I + L +E++ P E++++Y G V E + ++ I++ G Sbjct: 7 EILT-WLTNMGYAGIAIGLMIEII----PSEIVLAYGGYMVSEGTIGFIGAIIAGVIGGT 61 Query: 62 LGVTLSYWVGYRLGRPFIARYGARIHLTEERIVKMNRWFEKYGDKLLFVAYFIPGVRHIT 121 + YW+G GRPF+ +YG + + + I WF+KYG ++F A FIP VRH Sbjct: 62 IAQIFIYWIGRYGGRPFLDKYGKYLLIKKHHIDMSENWFQKYGAGVVFSARFIPVVRHAI 121 Query: 122 GYFCGVTRMPFRRYALFAYSGAIFWVSLFISLGKVLGPKWEQYHSTVNRYLIIFTVVSIL 181 G+ RMPF ++ + I W LF+ LG LG +W+ + Y +++++ Sbjct: 122 SIPAGIARMPFLKFVVLTVLAIIPWSILFVYLGIQLGSQWDDVENIAGTYTTPIMILAVV 181 Query: 182 LTAVIYLYRR 191 + A+ ++ ++ Sbjct: 182 VIALYFVIKK 191 >all4799 [S] COG0586 Uncharacterized membrane-associated protein Length = 209 Score = 90.9 bits (224), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 58/192 (30%), Positives = 95/192 (49%), Gaps = 10/192 (5%) Query: 1 LDILTHLLEHYGYGLIFLFLCLEMLALPLPGEMLMSYIGLSVYEQKLSWMLCIVSAGTGV 60 L+ + YGY IFL + LE L LP+PGE + G +LS+ L + A TG Sbjct: 8 LENIQEFAHQYGYWAIFLGILLENLGLPIPGETVTLVGGFLAGSDELSYWLVLGVAVTGA 67 Query: 61 LLGVTLSYWVGYRLGRPFIARYGARIHLTEERIVKMNRWFEKYGDKLLFVAYFIPGVRHI 120 ++G YW+G G PF+ + G ++EE+++ + F + K +F F +R Sbjct: 68 VIGANCGYWIGKLGGWPFLLQIGKIFRISEEKLLNIKEQFSQNAAKAVFFGRFFALLRIF 127 Query: 121 TGYFCGVTRMPFRRYALFAYSGAIFWVSLFISL----GKVLGPKWEQYHSTVNRYLIIFT 176 G+ MPF ++ + +GA W SL ++L GKV+ EQ + V + F Sbjct: 128 AAPLAGIAEMPFGKFFAYNLAGATAWASLMVTLAFFAGKVI--SLEQLVAWVGQ----FA 181 Query: 177 VVSILLTAVIYL 188 +V++L+ A + L Sbjct: 182 IVAVLILAALIL 193 >slr0509 [S] COG0586 Uncharacterized membrane-associated protein Length = 205 Score = 89.0 bits (219), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 59/190 (31%), Positives = 92/190 (48%), Gaps = 8/190 (4%) Query: 4 LTHLLEHYGYGLIFLFLCLEMLALPLPGEMLMSYIGLSVYEQKLSWMLCIVSAGTGVLLG 63 + ++ GYG I L + LE L P+P E++M G +V + KL IV+ G +LG Sbjct: 9 IPQVMNQLGYGGISLLMFLENLFPPIPSELIMPLAGFAVAQGKLELFPAIVAGIIGTILG 68 Query: 64 VTLSY----WVGYRLGRPFIARYGARIHLTEERIVKMNRWFEKYGDKLLFVAYFIPGVRH 119 Y WV RYG I L + I K N WF +YG + +F +PG+R Sbjct: 69 AYPWYYIGKWVSEERLEQLADRYGKWIGLDAKDIHKSNVWFGRYGHQSVFFGRLVPGIRT 128 Query: 120 ITGYFCGVTRMPFRRYALFAYSGAIFWVSLFISLGKVLGPKW---EQYHSTVNRYLIIFT 176 I GV M + L++ G WV+ S G LG + EQY V++ +++ + Sbjct: 129 IVSLPAGVNAMGLISFTLYSLGGISLWVTFLASAGYKLGDHYELVEQYLGPVSK-IVLVS 187 Query: 177 VVSILLTAVI 186 +V+IL+ ++ Sbjct: 188 IVAILVLLIV 197 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.332 0.147 0.471 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,643,144 Number of extensions: 1366290 Number of successful extensions: 4930 Number of sequences better than 1.0e-05: 73 Number of HSP's gapped: 4903 Number of HSP's successfully gapped: 73 Length of query: 428 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 319 Effective length of database: 25,807,025 Effective search space: 8232440975 Effective search space used: 8232440975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 117 (49.7 bits)